A semantically adaptable integrated visualization and natural exploration of multi-scale biomedical data

The exploration of biomedical data which involves heterogeneous sources coming from different spatial scales and medical domains is a challenging topic in current research. In this work, we combine efforts regarding multi-scale visualization, multimodal interaction and knowledge formalization for the exploration of multi-scale biomedical data. The knowledge formalization stores and organizes the information sources, the integrated visualization captures all relevant information for the domain expertise of the user and the multimodal interaction provides a natural exploration. We present a concrete example of use of the proposed exploratory system designed for a biologist investigating multi-scale pathologies.This work was supported from the EU Marie Curie ITN MultiScaleHuman (FP7-PEOPLE-2011-ITN, Grant agreement no.: 289897). The authors would like to thank all the partners for providing biomedical data sets.info:eu-repo/semantics/publishedVersio

Verfahren zum Steuern eines Fahrzeugs, Vorrichtung zum Erzeugen von Steuersignalen für ein Fahrzeug und Fahrzeug

EP2881829A2 [DE] Ein Verfahren zum Steuern eines Fahrzeugs von einer aktuellen Position in die Naehe einer Zielposition umfasst das Bestimmen (S102) des Befahrungsrisikos einer Mehrzahl von Positionen in einer Umgebung des Fahrzeugs zu einem aktuellen Zeitpunkt und zu mehreren, dem aktuellen Zeitpunkt folgenden Zeitpunkten und das Bestimmen einer Trajektorie fuer das Fahrzeug, die die aktuelle Position und die Zielposition verbindet oder naeherungsweise verbindet, unter Beruecksichtigung von errechneten Befahrungsrisiken (etwa basierend auf Kollisionswahrscheinlichkeiten, Verkehrsregeln) sowie Fahrdynamik- und Komfortparametern. Das Fahrzeugs wird dann entlang der modifizierten Trajektorie gesteuert (S110)

Improved fluorescence assays to measure the defects associated with F508del-CFTR allow identification of new active compounds

BACKGROUND AND PURPOSE: Cystic fibrosis (CF) is a debilitating disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, which codes for a Cl-/HCO3 - channel. F508del, the most common CF-associated mutation, causes both gating and biogenesis defects in the CFTR protein. This paper describes the optimisation of two fluorescence assays, capable of measuring CFTR function and cellular localisation, and their use in a pilot drug screen. EXPERIMENTAL APPROACH: HEK293 cells expressing YFP-F508del-CFTR, in which halide sensitive YFP is tagged to the N-terminal of CFTR, were used to screen a small library of compounds based on the VX-770 scaffold. Cells expressing F508del-CFTR-pHTomato, in which a pH sensor is tagged to the fourth extracellular loop of CFTR, were used to measure CFTR plasma membrane exposure following chronic treatment with the novel potentiators. KEY RESULTS: Active compounds with efficacy ~50% of VX-770, micromolar potency, and structurally distinct from VX-770 were identified in the screen. The F508del-CFTR-pHTomato assay suggests that the hit compound MS131A, unlike VX-770, does not decrease membrane exposure of F508del-CFTR. CONCLUSIONS AND IMPLICATIONS: Negative influence on F508del-CFTR biogenesis/stability by most known potentiators requires membrane exposure to be monitored early during development of drugs targeting CFTR. Combined use of the two fluorescence assays described here provides a useful tool for the identification of improved potentiators and correctors. The assays could also prove useful for basic scientific investigation on F508del-CFTR, and other CF-causing mutations