Verso un sistema energetico italiano basato sulle fonti rinnovabili. Prima parte: analisi introduttiva, problematiche e scenari propedeutici

Scopo di questo lavoro è valutare la generale fattibilità di un sistema energetico italiano completamente basato su fonti energetiche rinnovabili. In particolare, si sono volute quantificare le dimensioni necessarie per l’apporto di fotovoltaico ed eolico,e le problematiche legate alla intermittenza di queste fonti, sia su scala circadiana che annuale. Si è confrontato quindi un ipotetico profilo di produzione, basato sui dati reali di produttività degli impianti esistenti per ciascuna delle 8760 ore che compongono un anno, con il profilo di consumo derivato, con alcune assunzioni, da quello reale del 2019.Il confronto è basato su un modello e su assunzioni differenti in relazione alle possibili strategie per affrontare il problema.Il lavoro non èquindi un piano energetico o il progetto di un sistema energetico reale, ma solamente uno strumento per fornire indicazioni quantitative sulle dimensioni attese e sui problemi che questo sistema dovrà affrontare

Development of a New Tool for 3D Modeling for Regenerative Medicine

The effectiveness of therapeutic treatment based on regenerative medicine for degenerative diseases (i.e., neurodegenerative or cardiac diseases) requires tools allowing the visualization and analysis of the three-dimensional (3D) distribution of target drugs within the tissue. Here, we present a new computational procedure able to overcome the limitations of visual analysis emerging by the examination of a molecular signal within images of serial tissue/organ sections by using the conventional techniques. Together with the 3D anatomical reconstitution of the tissue/organ, our framework allows the detection of signals of different origins (e.g., marked generic molecules, colorimetric, or fluorimetric substrates for enzymes; microRNA; recombinant protein). Remarkably, the application does not require the employment of specific tracking reagents for the imaging analysis. We report two different representative applications: the first shows the reconstruction of a 3D model of mouse brain with the analysis of the distribution of the β-Galactosidase, the second shows the reconstruction of a 3D mouse heart with the measurement of the cardiac volume

A Bird\u27s-Eye View of the Multiple Biochemical Mechanisms that Propel Pathology of Alzheimer\u27s Disease: Recent Advances and Mechanistic Perspectives on How to Halt the Disease Progression Targeting Multiple Pathways.

Neurons consume the highest amount of oxygen, depend on oxidative metabolism for energy, and survive for the lifetime of an individual. Therefore, neurons are vulnerable to death caused by oxidative-stress, accumulation of damaged and dysfunctional proteins and organelles. There is an exponential increase in the number of patients diagnosed with neurodegenerative diseases such as Alzheimer’s (AD) as the number of elderly increases exponentially. Development of AD pathology is a complex phenomenon characterized by neuronal death, accumulation of extracellular amyloid-β plaques and neurofibrillary tangles, and most importantly loss of memory and cognition. These pathologies are most likely caused by mechanisms including oxidative stress, mitochondrial dysfunction/stress, accumulation of misfolded proteins, and defective organelles due to impaired proteasome and autophagy mechanisms. Currently, there are no effective treatments to halt the progression of this disease. In order to treat this complex disease with multiple biochemical pathways involved, a complex treatment regimen targeting different mechanisms should be investigated. Furthermore, as AD is a progressive disease-causing morbidity over many years, any chemo-modulator for treatment must be used over long period of time. Therefore, treatments must be safe and non-interfering with other processes. Ideally, a treatment like medicinal food or a supplement that can be taken regularly without any side effect capable of reducing oxidative stress, stabilizing mitochondria, activating autophagy or proteasome, and increasing energy levels of neurons would be the best solution. This review summarizes progress in research on different mechanisms of AD development and some of the potential therapeutic development strategies targeting the aforementioned pathologies

GT2007-28063 NUMERICAL ASSESSMENT OF SCAP: A PASSIVE SYSTEM FOR PREVENTING THERMOACOUSTIC OSCILLATIONS IN GAS TURBINE ANNULAR COMBUSTORS

ABSTRACT ENEL operates a dozen combined cycle units whose V94.3A gas turbines are equipped with annular combustors. In such lean premixed gas turbines, particular operation conditions could trigger large pressure oscillations due to thermoacoustic instabilities. The ENEL Research unit is studying this phenomenon in order to find out methods which could avoid or mitigate such events. The use of effective numerical analysis techniques allowed us to investigate the realistic time evolution and behaviour of the acoustic fields associated with this phenomenon. KIEN, an in-house low diffusive URANS code capable of simulating 3D reactive flows, has been used in the Very Rough Grid approach. This approach permits the simulation, with a reasonable computational time, of quite long real transients with a computational domain extended over all the resonant volumes involved in the acoustic phenomenon. The V94.3A gas turbine model was set up with a full combustor 3D grid, going from the compressor outlet up to the turbine inlet, including both the annular plenum and the annular combustion chamber. The grid extends over the entire circular angle, including all the 24 premixed burners. Numerical runs were performed with the normal V94.3A combustor configuration, with input parameters set so as no oscillations develop in the standard ambient conditions. Wide pressure oscillations on the contrary are associated with the circumferential acoustic modes of the combustor, which have their onset and grow when winter ambient conditions are assumed. These results also confirmed that the sustaining mechanism is based on the equivalence ratio fluctuation of premix mixture and that plenum plays an important role in such mechanism. Based on these findings, a system for controlling the thermoacoustic oscillation has been conceived (Patent Pending), which acts on the plenum side of the combustor. This system, called SCAP (Segmentation of Combustor Annular Plenum), is based on the subdivision of the plenum annular volume by means of a few meridionally oriented walls. Repetition of KIEN runs with a SCAP configuration, in which a suitable number of segmentation walls were properly arranged in the annular plenum, demonstrated the effectiveness of this solution in preventing the development of wide thermoacoustic oscillations in the combustor

Biomechanical behaviour of native and sutured bronchi. An in-vitro study

BACKGROUND: Biomechanical behaviour evaluation of a suture is an important information for the surgeon to choose the best technique to perform. OBJECTIVE: To assess the biomechanical behavior of the native and mechanically sutured bronchi. METHODS: Ten bronchi were harvested from slaughtered pigs and then randomly separated in two groups, a control intact group and a sutured group where specimens were cut in half and sutured, to evaluate mechanical properties during a tensile test using a loading frame machine. In addition optoelectric motion tracking system was used to evaluate suture profile motion during the test. RESULTS: Significant differences (p < 0.05) were found between the two groups for the parameters investigated. The control group showed a higher maximal stress resistance and stiffness than the suture group, while elongation at rupture was increased in the sutured group. All the sutures broke in symmetric manner, as the mean of the side difference of the sutured specimens was 0.93 ± 0.80 mm at rupture. CONCLUSIONS: Biomechanical behaviour of native and sutured bronchi was evaluated, giving highly reproducible parameters regarding mechanical properties that may help clinicians and bioengineers to rationalize the choice for a particular suture material or suture technique, increasing surgical outcomes

www.mdpi.com/journal/jfb/ Review Mechanotransduction: Tuning Stem Cells Fate

Abstract: It is a general concern that the success of regenerative medicine-based applications is based on the ability to recapitulate the molecular events that allow stem cells to repair the damaged tissue/organ. To this end biomaterials are designed to display properties that, in a precise and physiological-like fashion, could drive stem cell fate both in vitro and in vivo. The rationale is that stem cells are highly sensitive to forces and that they may convert mechanical stimuli into a chemical response. In this review, we describe novelties on stem cells and biomaterials interactions with more focus on the implication of the mechanical stimulation named mechanotransduction

Efficient siRNA Delivery by the Cationic Liposome DOTAP in Human Hematopoietic Stem Cells Differentiating into Dendritic Cells

RNA interference technology is an ideal strategy to elucidate the mechanisms associated with human CD34+ hematopoietic stem cell differentiation into dendritic cells. Simple manipulations in vitro can unequivocally yield alloreactive or tolerogenic populations, suggesting key implications of biochemical players that might emerge as therapeutic targets for cancer or graft-versus-host disease. To knockdown proteins typically involved in the biology of dendritic cells, we employed an siRNA delivery system based on the cationic liposome DOTAP as the carrier. Freshly-isolated CD34+ cells were transfected with siRNA for cathepsin S with negligible cytotoxicity and transfection rates (>60%) comparable to the efficiency shown by lentiviral vectors. Further, cathepsin S knockdown was performed during both cell commitment and through the entire 14-day differentiation process with repeated transfection rounds that had no effect per se on cell development. Tested in parallel, other commercially-available chemical reagents failed to meet acceptable standards. In addition to safe and practical handling, a direct advantage of DOTAP over viral-mediated techniques is that transient silencing effects can be dynamically appraised through the recovery of targeted proteins. Thus, our findings identify DOTAP as an excellent reagent for gene silencing in resting and differentiating CD34+ cells, suggesting a potential for applications in related preclinical models