PLZF mutation alters mouse hematopoietic stem cell function and cell cycle progression

International audienceKey Points Inactivation of PLZF promotes phenotype of HSC aging. PLZF controls HSC cell cycle

Regulation of the positive transcriptional effect of PLZF through a non-canonical EZH2 activity

International audienceThe transcription factor PLZF (promyelocytic leukemia zinc finger protein) acts as an epigenetic regulator balancing self-renewal and differentiation of hematopoietic cells through binding to various chromatin-modifying factors. First described as a transcriptional repressor, PLZF is also associated with active transcription, although the molecular bases underlying the differences are unknown. Here, we reveal that in a hematopoietic cell line, PLZF is predominantly associated with transcribed genes. Additionally, we identify a new association between PLZF and the histone methyltransferase, EZH2 at the genomic level. We find that co-occupancy of PLZF and EZH2 on chromatin at PLZF target genes is not associated with SUZ12 or trimethylated lysine 27 of histone H3 (H3K27me3) but with the active histone mark H3K4me3 and active transcription. Removal of EZH2 leads to an increase of PLZF binding and increased gene expression. Our results suggest a new role of EZH2 in restricting PLZF positive transcriptional activity independently of its canonical PRC2 activity

Wireless ventilation control for large-scale systems: the mining industrial case

This paper describes a new industrial case on automation, for large scale systems with high environmental impact: the mining ventilation control systems. Ventilation control is essential for the operation of a mine in terms of safety (CO and NOx regulation) and energy optimization. We first discuss a novel regulation architecture, highlighting the interest for a model-based control approach and the use of distributed sensing capabilities thanks to a wireless sensor network (WSN). We propose a new model for underground ventilation. The main components of the system dynamics are described with time-delays, transmission errors, energy losses and concentration profiles. Two different modelbased control approaches, which can embody the complex dynamics of the system, are proposed. The first one resorts to a nonlinear model predictive control strategy (receding horizon) and aims to energy minimization thanks to a continuous operation of the fans. The second one, based on a hybrid description of the model and fans operation, provides automatic verification of the wireless control thanks to abstraction techniques. These control strategies are compared with simulations, in terms of regulation efficiency, energy consumption and need for computational capabilities. The industrial case description and control strategies open new vistas for the development of global system approaches that allow for the optimization of energy consumption of complex large-scale systems. Copyright c

Great African Rift Interdisciplinary Research Group 2022 Scientific meeting

Welcome to the GDR Rift Scientific Colloquium 2022 The GDR Rift is pleased to present this first scientific colloquium which took place from 22 to 24 November 2022, at the Villemanzy residence, in Lyon. This conference was open to all researchers, students, and staff involved in the GDR, wishing to address issues related to the Great African Rift in an interdisciplinary approach. This meeting was dedicated to the presentation of the latest scientific results in or on the Great African Rift during plenary sessions, but also allowed for exchange and discussion during targeted workshops in smaller groups. Presentations were given in English, and plenary sessions were broadcast on video

Great African Rift Interdisciplinary Research Group 2022 Scientific meeting

Welcome to the GDR Rift Scientific Colloquium 2022 The GDR Rift is pleased to present this first scientific colloquium which took place from 22 to 24 November 2022, at the Villemanzy residence, in Lyon. This conference was open to all researchers, students, and staff involved in the GDR, wishing to address issues related to the Great African Rift in an interdisciplinary approach. This meeting was dedicated to the presentation of the latest scientific results in or on the Great African Rift during plenary sessions, but also allowed for exchange and discussion during targeted workshops in smaller groups. Presentations were given in English, and plenary sessions were broadcast on video

Great African Rift Interdisciplinary Research Group 2022 Scientific meeting

Welcome to the GDR Rift Scientific Colloquium 2022 The GDR Rift is pleased to present this first scientific colloquium which took place from 22 to 24 November 2022, at the Villemanzy residence, in Lyon. This conference was open to all researchers, students, and staff involved in the GDR, wishing to address issues related to the Great African Rift in an interdisciplinary approach. This meeting was dedicated to the presentation of the latest scientific results in or on the Great African Rift during plenary sessions, but also allowed for exchange and discussion during targeted workshops in smaller groups. Presentations were given in English, and plenary sessions were broadcast on video

Fonctions, utilisations et représentations de l’espace dans les sépultures monumentales du Néolithique européen

De Newgrange en Irlande à Hal Saflieni à Malte ou Gavrinis en Bretagne, les tombeaux néolithiques sont connus pour leur caractère monumental. Loin d’être de simples “contenants” mortuaires, destinés uniquement à recueillir les restes corporels des défunts, ces tombes sont des architectures complexes et multiples, conçues pour accueillir des rites funéraires élaborés, impliquant plusieurs acteurs, plusieurs temps et plusieurs espaces cérémoniels. Pour l’archéologue, l’organisation spatiale de ces architectures et de leurs contenants constitue un véritable fil conducteur permettant de comprendre les pratiques funéraires et les croyances des sociétés néolithiques. Cet ouvrage résulte d’une rencontre scientifique organisée du 8 au 10 juin 2011 à la Maison Méditerranéenne des Sciences de l’Homme, à Aix-en-Provence, et rassemble les contributions d’une trentenaire de spécialistes européens (France, Grande-Bretagne, Irlande, Espagne, Italie, Pologne). Combinant études récentes et des synthèses régionales, il explore la configuration de l’architecture de ces tombes (chambres mégalithiques, hypogées, coffres sous tumulus ou cairns, tertres, etc.), l’organisation spatiale des dépôts funéraires et des décors pariétaux, et les relations entre les tombes et leur espace topographique naturel (le paysage). Ce tour d’horizon européen expose ainsi les différentes manières dont les sociétés néolithiques concevaient, construisaient et utilisaient l’espace des morts en Europe de 5000 à 2000 avant J.-C. Mais il offre également des pistes permettant d’interpréter ces choix. Quelles sont les fonctions des différents espaces architecturaux ? Quel est le rôle de l’espace naturel autour des tombes ? En quoi ces rapports particuliers à l’espace nous renseignent-ils sur les structures et pratiques sociales des populations néolithiques européennes, sur leurs conceptions symboliques et leurs représentations de l’espace funéraire ? From Newgrange in Ireland to Hal Saflieni in Malta or Gavrinis in Brittany, Neolithic tombs are well known for their monumental nature. Far from being simple mortuary “containers”, only aimed at receiving dead bodies, these tombs are complex and multiple architectures that were designed to host elaborate burial rituals involving several actors and several ceremonial times and spaces. For archaeologists, the spatial organisation of these architectures and their contents is a thread to investigate and understand the burial practices and beliefs of Neolithic communities. This volume results from a conference held at the Maison Méditerranéenne des Sciences de l’Homme in Aix-en-Provence on June 8th–10th 2011, and brings together contributions from 30 European specialists from France, UK, Ireland, Spain, Italy, and Poland. Combining recent fieldworks and regional surveys, it explores the configuration of tombs architecture (megalithic chambered tombs, rock-cut tombs, cists under tumulus or cairns, barrows, etc.), the spatial organisation of bodies, grave goods and imagery, and the relationships between the tombs and their landscape setting. This European overview shows the different ways Neolithic societies thought, built and used the space of the dead in Europe from 5000 to 2000 BC. But it also offers and discusses ideas about the meaning of these spatial choices. What are the functions of the different architectural spaces within tombs? What was the role of natural landscape features around the monuments? What spatial patterns in tomb construction and use can tell us about social structures and practices during the Neolithic, and about the symbolic conceptions of Neolithic societies and their representations of the space of the dead