The influence of the cylindrical shape of the nucleosomes and H1 defects on properties of chromatin

We present a model improving the two-angle model for interphase chromatin (E2A model). This model takes into account the cylindrical shape of the histone octamers, the H1 histones in front of the nucleosomes and the vertical distance $d$ between the in and outgoing DNA strands. Factoring these chromatin features in, one gets essential changes in the chromatin phase diagram: Not only the shape of the excluded-volume borderline changes but also the vertical distance $d$ has a dramatic influence on the forbidden area. Furthermore, we examined the influence of H1 defects on the properties of the chromatin fiber. Thus we present two possible strategies for chromatin compaction: The use of very dense states in the phase diagram in the gaps in the excluded volume borderline or missing H1 histones which can lead to very compact fibers. The chromatin fiber might use both of these mechanisms to compact itself at least locally. Line densities computed within the model coincident with the experimental values

Ovine Enzootic Abortion (OEA): a comparison of antibody responses in vaccinated and naturally-infected swiss sheep over a two year period

Background Prevention and control of ovine enzootic abortion (OEA) can be achieved by application of a live vaccine. In this study, five sheep flocks with different vaccination and infection status were serologically tested using a competitive enzyme-linked immunosorbent assay (cELISA) specific for Chlamydophila (Cp.) abortus over a two-year time period. Results Sheep in Flock A with recent OEA history had high antibody values after vaccination similar to Flock C with natural Cp. abortus infections. In contrast, OEA serology negative sheep (Flock E) showed individual animal-specific immunoreactions after vaccination. Antibody levels of vaccinated ewes in Flock B ranged from negative to positive two and three years after vaccination, respectively. Positive antibody values in the negative control Flock D (without OEA or vaccination) are probably due to asymptomatic intestinal infections with Cp. abortus. Excretion of the attenuated strain of Cp. abortus used in the live vaccine through the eye was not observed in vaccinated animals of Flock E. Conclusion The findings of our study indicate that, using serology, no distinction can be made between vaccinated and naturally infected sheep. As a result, confirmation of a negative OEA status in vaccinated animals by serology cannot be determined

Development of a GEM-TPC prototype

The use of GEM foils for the amplification stage of a TPC instead of a con- ventional MWPC allows one to bypass the necessity of gating, as the backdrift is suppressed thanks to the asymmetric field configuration. This way, a novel continuously running TPC, which represents one option for the PANDA central tracker, can be realized. A medium sized prototype with a diameter of 300 mm and a length of 600 mm will be tested inside the FOPI spectrometer at GSI using a carbon or lithium beam at intermediate energies (E = 1-3AGeV). This detector test under realistic experimental conditions should allow us to verify the spatial resolution for single tracks and the reconstruction capability for displaced vertexes. A series of physics measurement implying pion beams is scheduled with the FOPI spectrometer together with the GEM-TPC as well.Comment: 5 pages, 4 figures, Proceedings for 11th ICATTP conference in como (italy

Flow and retreat of the Late Quaternary Pine Island-Thwaites palaeo-ice stream, West Antarctica

Multibeam swath bathymetry and sub-bottom profiler data are used to establish constraints on the flow and retreat history of a major palaeo-ice stream that carried the combined discharge from the parts of the West Antarctic Ice Sheet now occupied by the Pine Island and Thwaites glacier basins. Sets of highly elongated bedforms show that, at the last glacial maximum, the route of the Pine Island-Thwaites palaeo-ice stream arced north-northeast following a prominent cross-shelf trough. In this area, the grounding line advanced to within similar to 68 km of, and probably reached, the shelf edge. Minimum ice thickness is estimated at 715 m on the outer shelf, and we estimate a minimum ice discharge of similar to 108 km(3) yr(-1) assuming velocities similar to today's Pine Island glacier (similar to 2.5 km yr(-1)). Additional bed forms observed in a trough northwest of Pine Island Bay likely formed via diachronous ice flows across the outer shelf and demonstrate switching ice stream behavior. The "style" of ice retreat is also evident in five grounding zone wedges, which suggest episodic deglaciation characterized by halts in grounding line migration up-trough. Stillstands occurred in association with changes in ice bed gradient, and phases of inferred rapid retreat correlate to higher bed slopes, supporting theoretical studies that show bed geometry as a control on ice margin recession. However, estimates that individual wedges could have formed within several centuries still imply a relatively rapid overall retreat. Our findings show that the ice stream channeled a substantial fraction of West Antarctica's discharge in the past, just as the Pine Island and Thwaites glaciers do today

Monte Carlo Simulations indicate that Chromati: Nanostructure is accessible by Light Microscopy

A long controversy exists about the structure of chromatin. Theoretically, this structure could be resolved by scattering experiments if one determines the scattering function - or equivalently the pair distribution function - of the nucleosomes. Unfortunately, scattering experiments with live cells are very difficult and limited to only a couple of nucleosomes

Hadron structure at low Q^2

This review deals with the structure of hadrons, strongly interacting many-body systems consisting of quarks and gluons. These systems have a size of about 1 fm, which shows up in scattering experiments at low momentum transfers $Q$ in the GeV region. At this scale the running coupling constant of Quantum Chromodynamics (QCD), the established theory of the strong interactions, becomes divergent. It is therefore highly intriguing to explore this theory in the realm of its strong interaction regime. However, the quarks and gluons can not be resolved at the GeV scale but have to be studied through their manifestations in the bound many-body systems, for instance pions, nucleons and their resonances. The review starts with a short overview of QCD at low momentum transfer and a summary of the theoretical apparatus describing the interaction of hadrons with electrons and photons. In the following sections we present the experimental results for the most significant observables studied with the electromagnetic probe: form factors, polarizabilities, excitation spectra, and sum rules. These experimental findings are compared and interpreted with various theoretical approaches to QCD, such as phenomenological models with quarks and pions, dispersion relations as a means to connect observables from different experiments, and, directly based on the QCD lagrangian, chiral perturbation theory and lattice gauge theory.Comment: 55 pages, 47 figures, revie

Atmosphere-ocean-ice interactions in the Amundsen Sea Embayment, West Antarctica

Over recent decades outlet glaciers of the Amundsen Sea Embayment (ASE), West Antarctica, have accelerated, thinned and retreated, and are now contributing approximately 10% to global sea level rise. All the ASE glaciers flow into ice shelves, and it is the thinning of these since the 1970s, and their ungrounding from “pinning points” that is widely held to be responsible for triggering the glaciers’ decline. These changes have been linked to the inflow of warm Circumpolar Deep Water (CDW) onto the ASE's continental shelf. CDW delivery is highly variable, and is closely related to the regional atmospheric circulation. The ASE is south of the Amundsen Sea Low (ASL), which has a large variability and which has deepened in recent decades. The ASL is influenced by the phase of the Southern Annular Mode, along with tropical climate variability. It is not currently possible to simulate such complex atmosphere-ocean-ice interactions in models, hampering prediction of future change. The current retreat could mark the beginning of an unstable phase of the ASE glaciers that, if continued, will result in collapse of the West Antarctic Ice Sheet, but numerical ice-sheet models currently lack the predictive power to answer this question. It is equally possible that the recent retreat will be short-lived and that the ASE will find a new stable state. Progress is hindered by incomplete knowledge of bed topography in the vicinity of the grounding line. Furthermore, a number of key processes are still missing or poorly represented in models of ice-flow