Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food

In this study, the primers and the probe were designed, and a completely new real-time polymerase chain reaction (PCR) protocol for detecting the invA gene of Salmonella in food was optimised and validated in-house. The inclusivity test used 76 different Salmonella isolates with no false-negative results. The exclusivity was tested using 45 non-Salmonella microorganisms with no false-positive results. The method was also successfully applied while examining five different artificially contaminated food categories. The results were compared to the standard method (ISO 6579-1) and two previously validated real-time PCR methods. The developed assay is sensitive and specific for rapidly detecting Salmonella in food

Geographical and temporal distribution of SARS-CoV-2 clades in the WHO European Region, January to June 2020

We show the distribution of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) genetic clades over time and between countries and outline potential genomic surveillance objectives. We applied three genomic nomenclature systems to all sequence data from the World Health Organization European Region available until 10 July 2020. We highlight the importance of real-time sequencing and data dissemination in a pandemic situation, compare the nomenclatures and lay a foundation for future European genomic surveillance of SARS-CoV-2

Geographical and temporal distribution of SARS-CoV-2 clades in the WHO European Region, January to June 2020

We show the distribution of SARS-CoV-2 genetic clades over time and between countries and outline potential genomic surveillance objectives. We applied three available genomic nomenclature systems for SARS-CoV-2 to all sequence data from the WHO European Region available during the COVID-19 pandemic until 10 July 2020. We highlight the importance of real-time sequencing and data dissemination in a pandemic situation. We provide a comparison of the nomenclatures and lay a foundation for future European genomic surveillance of SARS-CoV-2.Peer reviewe

Rhabdomyolysis and acute renal failure in a child with parainfluenza type 1 infection

We present a rare case of para-influenza type 1 virus-induced rhabdomyolysis, complicated by acute renal failure (ARF). The child underwent continuous venovenous haemofiltration and has shown full clinical and biochemical recovery. ARF due to rhabdomyolysis in para-influenza type 1 infection in a child has, to the best of our knowledge, not been previously reported

Response of a Carbonate Platform to the Cenomanian-Turonian Drowning and OAE 2: A Case Study from the Adriatic Platform (Dalmatia, Croatia)

Global perturbations during the Cenomanian–Turonian boundary (CTB) interval and the Oceanic Anoxic Event 2 (OAE 2) represent one of the most extensively studied past environmental changes. To explore the response of various carbonate-platform depositional systems to such major environmental perturbations, strata of the intra-Tethyan Adriatic carbonate platform (sensu stricto) from the island of Bracˇ (Adriatic Sea, Croatia) provide excellent exposures and a previously well-established Upper Cretaceous lithostratigraphic framework. Within this context, this study integrated lithostratigraphy, biostratigraphy, and chemostratigraphy to describe a drowned-platform succession underlain and overlain by peritidal carbonates. Carbon-isotope stratigraphy of this succession revealed a shift towards positive d13C values that reached +4 to +5% VPDB, and represent the CTB interval excursion plateau. We observed variations in thickness of the drowned-platform successions and explained them by three superimposed mechanisms: (1) diachronous drowning of platform relief; (2) intra-platform redeposition of parts of the successions by various mass-gravity transport processes (indicating enhanced instability due to increasing accommodation space related to the late Cenomanian platform drowning and synsedimentary tectonics); and (3) migration of major carbonate factories during the recovery of shallow-platform environments. The results indicate that the CTB interval event caused unusual increase in accommodation space on the carbonate platform enabling open-marine influence and synsedimentary redeposition. However, widespread organic-rich black shales reported from coeval strata of other regions have not been documented from the platform-top successions to date, and were probably accumulated in deeper (anoxic) settings below the rising sea level