Whose Needs Does Service Serve? Complicating the Citizen Soldier Narrative

The growth of conscript militaries was integral to the creation of civil rights in European nation-states, which established militaries as a key site of claims-making. However, the United States military has diverged from these models, and most cases of inclusion or integration of social groups are not directly connected with claims-making. What has influenced the U.S. military’s responsiveness to pressures, both internal and external, and how has this changed over time? I employ a comparative historical approach to three cases—African-Americans, women, and non-heterosexuals—to unpack the U.S. military as a state institution and a site of claims-making. By incorporating elements unique to American institutions into existing models of militaries, I find that the U.S. military has become increasingly vulnerable to domestic political, international political, internal economic, and internal and external cultural pressures since the World War period. Despite its enormous economic and physical strength, the U.S. military is more responsive now than ever before to internal and external demands

Standing variation and new mutations both contribute to a fast response to selection for flowering time in maize inbreds

<p>Abstract</p> <p>Background</p> <p>In order to investigate the rate and limits of the response to selection from highly inbred genetic material and evaluate the respective contribution of standing variation and new mutations, we conducted a divergent selection experiment from maize inbred lines in open-field conditions during 7 years. Two maize commercial seed lots considered as inbred lines, <it>F</it>252 and <it>MBS</it>847, constituted two biological replicates of the experiment. In each replicate, we derived an Early and a Late population by selecting and selfing the earliest and the latest individuals, respectively, to produce the next generation.</p> <p>Results</p> <p>All populations, except the Early <it>MBS</it>847, responded to selection despite a short number of generations and a small effective population size. Part of the response can be attributed to standing genetic variation in the initial seed lot. Indeed, we identified one polymorphism initially segregating in the <it>F</it>252 seed lot at a candidate locus for flowering time, which explained 35% of the trait variation within the Late <it>F</it>252 population. However, the model that best explained our data takes into account both residual polymorphism in the initial seed lots and a constant input of heritable genetic variation by new (epi)mutations. Under this model, values of mutational heritability range from 0.013 to 0.025, and stand as an upper bound compare to what is reported in other species.</p> <p>Conclusions</p> <p>Our study reports a long-term divergent selection experiment for a complex trait, flowering time, conducted on maize in open-field conditions. Starting from a highly inbred material, we created within a few generations populations that strikingly differ from the initial seed lot for flowering time while preserving most of the phenotypic characteristics of the initial inbred. Such material is unique for studying the dynamics of the response to selection and its determinants. In addition to the fixation of a standing beneficial mutation associated with a large phenotypic effect, a constant input of genetic variance by new mutations has likely contributed to the response. We discuss our results in the context of the evolution and mutational dynamics of populations characterized by a small effective population size.</p

Genome Size and Transposable Element Content as Determined by High-Throughput Sequencing in Maize and Zea luxurians

The genome of maize (Zea mays ssp. mays) consists mostly of transposable elements (TEs) and varies in size among lines. This variation extends to other species in the genus Zea: although maize and Zea luxurians diverged only ∼140,000 years ago, their genomes differ in size by ∼50%. We used paired-end Illumina sequencing to evaluate the potential contribution of TEs to the genome size difference between these two species. We aligned the reads both to a filtered gene set and to an exemplar database of unique repeats representing 1,514 TE families; ∼85% of reads mapped against TE repeats in both species. The relative contribution of TE families to the B73 genome was highly correlated with previous estimates, suggesting that reliable estimates of TE content can be obtained from short high-throughput sequencing reads, even at low coverage. Because we used paired-end reads, we could assess whether a TE was near a gene by determining if one paired read mapped to a TE and the second read mapped to a gene. Using this method, Class 2 DNA elements were found significantly more often in genic regions than Class 1 RNA elements, but Class 1 elements were found more often near other TEs. Overall, we found that both Class 1 and 2 TE families account for ∼70% of the genome size difference between B73 and luxurians. Interestingly, the relative abundance of TE families was conserved between species (r = 0.97), suggesting genome-wide control of TE content rather than family-specific effects

QTL Mapping Combined With Comparative Analyses Identified Candidate Genes for Reduced Shattering in Setaria italica

Setaria (L.) P. Beauv is a genus of grasses that belongs to the Poaceae (grass) family, subfamily Panicoideae. Two members of the Setaria genus, Setaria italica (foxtail millet) and S. viridis (green foxtail), have been studied extensively over the past few years as model species for C4-photosynthesis and to facilitate genome studies in complex Panicoid bioenergy grasses. We exploited the available genetic and genomic resources for S. italica and its wild progenitor, S. viridis, to study the genetic basis of seed shattering. Reduced shattering is a key trait that underwent positive selection during domestication. Phenotyping of F2:3 and recombinant inbred line (RIL) populations generated from a cross between S. italica accession B100 and S. viridis accession A10 identified the presence of additive main effect quantitative trait loci (QTL) on chromosomes V and IX. As expected, enhanced seed shattering was contributed by the wild S. viridis. Comparative analyses pinpointed Sh1 and qSH1, two shattering genes previously identified in sorghum and rice, as potentially underlying the QTL on Setaria chromosomes IX and V, respectively. The Sh1 allele in S. italica was shown to carry a PIF/Harbinger MITE in exon 2, which gave rise to an alternatively spliced transcript that lacked exon 2. This MITE was universally present in S. italica accessions around the world and absent from the S. viridis germplasm tested, strongly suggesting a single origin of foxtail millet domestication. The qSH1 gene carried two MITEs in the 5′UTR. Presence of one or both MITEs was strongly associated with cultivated germplasm. If the MITE insertion(s) in qSH1 played a role in reducing shattering in S. italica accessions, selection for the variants likely occurred after the domestication of foxtail millet

New Insight into the History of Domesticated Apple: Secondary Contribution of the European Wild Apple to the Genome of Cultivated Varieties

The apple is the most common and culturally important fruit crop of temperate areas. The elucidation of its origin and domestication history is therefore of great interest. The wild Central Asian species Malus sieversii has previously been identified as the main contributor to the genome of the cultivated apple (Malus domestica), on the basis of morphological, molecular, and historical evidence. The possible contribution of other wild species present along the Silk Route running from Asia to Western Europe remains a matter of debate, particularly with respect to the contribution of the European wild apple. We used microsatellite markers and an unprecedented large sampling of five Malus species throughout Eurasia (839 accessions from China to Spain) to show that multiple species have contributed to the genetic makeup of domesticated apples. The wild European crabapple M. sylvestris, in particular, was a major secondary contributor. Bidirectional gene flow between the domesticated apple and the European crabapple resulted in the current M. domestica being genetically more closely related to this species than to its Central Asian progenitor, M. sieversii. We found no evidence of a domestication bottleneck or clonal population structure in apples, despite the use of vegetative propagation by grafting. We show that the evolution of domesticated apples occurred over a long time period and involved more than one wild species. Our results support the view that self-incompatibility, a long lifespan, and cultural practices such as selection from open-pollinated seeds have facilitated introgression from wild relatives and the maintenance of genetic variation during domestication. This combination of processes may account for the diversification of several long-lived perennial crops, yielding domestication patterns different from those observed for annual species

Superheroes and masterminds of plant domestication

AbstractDomestication is one of the most fundamental changes in the evolution of human societies. The geographical origins of domesticated plants are inferred from archaeology, ecology and genetic data. Scenarios vary among species and include single, diffuse or multiple independent domestications. Cultivated plants present a panel of traits, the “domestication syndrome” that distinguish them from their wild relatives. It encompasses yield-, food usage-, and cultivation-related traits. Most genes underlying those traits are “masterminds” affecting the regulation of gene networks. Phenotypic convergence of domestication traits across species or within species between independently domesticated forms rarely coincides with convergence at the gene level. We review here current data/models that propose a protracted transition model for domestication and investigate the impact of mating system, life cycle and gene flow on the pace of domestication. Finally, we discuss the cost of domestication, pointing to the importance of characterizing adaptive functional variation in wild resources

RIDGE, a tool tailored to detect gene flow barriers across species pairs

Abstract Characterizing the processes underlying reproductive isolation between diverging lineages is central to understanding speciation. Here, we present RIDGE – Reproductive Isolation Detection using Genomic polymorphisms – a tool tailored for quantifying gene flow barrier proportions and identifying the corresponding genomic regions. RIDGE relies on an Approximate Bayesian Computation with a model-averaging approach to accommodate diverse scenarios of lineage divergence. It captures heterogeneity in effective migration rate along the genome while accounting for variation in linked selection and recombination. The barrier detection test relies on numerous summary statistics to compute a Bayes factor, offering a robust statistical framework that facilitates cross-species comparisons. Simulations revealed that RIDGE is particularly efficient both at capturing signals of ongoing migration and at identifying barrier loci, including for recent divergence times (~0.1 2 N e generations). Applying RIDGE to four published crow datasets, we validated our tool by identifying a well-known large genomic region associated with mate choice patterns. We identified additional barrier loci between species pairs, which have shown, on the one hand, that depending on the biological, demographic, and selection contexts, different combinations of summary statistics are informative for the detection of signals. On the other hand, these analyses also highlight the value of our newly developed outlier statistics in challenging detection conditions

Population Structure and Its Effects on Patterns of Nucleotide Polymorphism in Teosinte (Zea mays ssp. parviglumis)

Surveys of nucleotide diversity in the wild ancestor of maize, Zea mays ssp. parviglumis, have revealed genomewide departures from the standard neutral equilibrium (NE) model. Here we investigate the degree to which population structure may account for the excess of rare polymorphisms frequently observed in species-wide samples. On the basis of sequence data from five nuclear and two chloroplast loci, we found significant population genetic structure among seven subpopulations from two geographic regions. Comparisons of estimates of population genetic parameters from species-wide samples and subpopulation-specific samples showed that population genetic subdivision influenced observed patterns of nucleotide polymorphism. In particular, Tajima's D was significantly higher (closer to zero) in subpopulation-specific samples relative to species-wide samples, and therefore more closely corresponded to NE expectations. In spite of these overall patterns, the extent to which levels and patterns of polymorphism within subpopulations differed from species-wide samples and NE expectations depended strongly on the geographic region (Jalisco vs. Balsas) from which subpopulations were sampled. This may be due to the demographic history of subpopulations in those regions. Overall, these results suggest that explicitly accounting for population structure may be important for studies examining the genetic basis of ecologically and agronomically important traits as well as for identifying loci that have been the targets of selection

raw AFLP/MSAP data for the MBS DSE

Raw AFLP/MSAP data recorded on each progenitor of the MBS DSE between generations G0 and G6

Molecular evolution accompanying functional divergence of duplicated genes along the plant starch biosynthesis pathway

Background: Starch is the main source of carbon storage in theArchaeplastida. The Starch Biosynthesis Pathway(SBP) emerged from cytosolic glycogen metabolism shortly after plastid endosymbiosis and was redirected to theplastid stroma during the green lineage divergence. The SBP is a complex network of genes, most of which aremembers of large multigene families. While some gene duplications occurred in theArchaeplastidaancestor, mostwere generated during the SBP redirection process, and the remaining few paralogs were generated throughcompartmentalization or tissue specialization during the evolution of the land plants. In the present study, wetested models of duplicated gene evolution in order to understand the evolutionary forces that have led to thedevelopment of SBP in angiosperms. We combined phylogenetic analyses and tests on the rates of evolution alongbranches emerging from major duplication events in six gene families encoding SBP enzymes.Results: We found evidence of positive selection along branches following cytosolic or plastidial specialization intwo starch phosphorylases and identified numerous residues that exhibited changes in volume, polarity or charge.Starch synthases, branching and debranching enzymes functional specializations were also accompanied byaccelerated evolution. However, none of the sites targeted by selection corresponded to known functionaldomains, catalytic or regulatory. Interestingly, among the 13 duplications tested, 7 exhibited evidence of positiveselection in both branches emerging from the duplication, 2 in only one branch, and 4 in none of the branches.Conclusions: The majority of duplications were followed by accelerated evolution targeting specific residues alongboth branches. This pattern was consistent with the optimization of the two sub-functions originally fulfilled by theancestral gene before duplication. Our results thereby provide strong support to the so-called“Escape fromAdaptive Conflict”(EAC) model. Because none of the residues targeted by selection occurred in characterizedfunctional domains, we propose that enzyme specialization has occurred through subtle changes in affinity, activityor interaction with other enzymes in complex formation, while the basic function defined by the catalytic domainhas been maintained