How Has the COVID-19 Pandemic Affected the Way We Access and Interact with the Countryside and the Animals within It?

SIMPLE SUMMARY: In March 2020, the UK entered its first mandatory lockdown to reduce the spread of COVID-19. The restrictions associated with the lockdown led to changes in human behaviour, particularly in relation to exercise and accessing the outdoors. This study used an online survey of UK residents to explore these changes and to understand how people interacted with the natural world while they were outside, including interactions with wildlife and domestic animals. There was an increase in how often respondents went for walks outside during lockdown, compared to pre-lockdown levels, and this increase lasted beyond the end of the first lockdown. Interacting with animals was a common feature of walks. This most commonly involved watching wildlife or domestic animals, such as livestock or horses, but sometimes also involved physically interacting with them and/or feeding them which may have implications for their welfare, for example, if inappropriate food is provided. There is also a risk of humans transferring disease between the animals they have contact with or zoonotic disease transmission between the humans and animals. While is it positive that people are interacting with the natural world, it is important that this does not compromise animal wellbeing. ABSTRACT: There is growing evidence that the changes in human behaviour resulting from the COVID-19 pandemic have had positive and negative impacts on the natural world. This study used an online survey to explore how the first UK lockdown affected human exercising behaviour, with particular focus on the role of wild and domestic animals. The survey was completed by 308 respondents. There was a significant increase in the frequency that respondents went for walks outdoors during lockdown, in comparison to pre-lockdown levels (p ≤ 0.001), and this was sustained (albeit to a lesser extent) once lockdown ended (p = 0.005). Engaging with the natural world was an important feature of walks outside for 81% of respondents. A small proportion of respondents reported physically interacting with the animals they encountered and/or feeding them, which may have implications for their welfare. The findings suggest that those who value animal encounters during their time outside always seek these interactions, while those who do not, did not tend to change this behaviour following lockdown. Should the changes in human exercising behaviour be sustained, it is important to balance the benefits of walking outdoors for human health and wellbeing with the health and welfare of the animals they encounter

Nucleoside Analogue Reverse Transcriptase Inhibitors Differentially Inhibit Human LINE-1 Retrotransposition

Intact LINE-1 elements are the only retrotransposons encoded by the human genome known to be capable of autonomous replication. Numerous cases of genetic disease have been traced to gene disruptions caused by LINE-1 retrotransposition events in germ-line cells. In addition, genomic instability resulting from LINE-1 retrotransposition in somatic cells has been proposed as a contributing factor to oncogenesis and to cancer progression. LINE-1 element activity may also play a role in normal physiology. LINE-1 retrotransposition reporter assay, we evaluated the abilities of several antiretroviral compounds to inhibit LINE-1 retrotransposition. The nucleoside analogue reverse transcriptase inhibitors (nRTIs): stavudine, zidovudine, tenofovir disoproxil fumarate, and lamivudine all inhibited LINE-1 retrotransposition with varying degrees of potencies, while the non-nucleoside HIV-1 reverse transcriptase inhibitor nevirapine showed no effect.Our data demonstrates the ability for nRTIs to suppress LINE-1 retrotransposition. This is immediately applicable to studies aimed at examining potential roles for LINE-1 retrotransposition in physiological processes. In addition, our data raises novel safety considerations for nRTIs based on their potential to disrupt physiological processes involving LINE-1 retrotransposition