Relação entre a condição corporal e fisiológica do lagostim vermelho da Louisiana (Procambarus clarkii) e a degradação ambiental do meio

O lagostim vermelho da Louisiana, Procambarus clarkii (Girard 1852), encontra-se amplamente distribuído por todo o Mundo e em locais com diversos níveis de qualidade ambiental. Neste trabalho, pretendeu-se avaliar a resposta fisiológica de P. clarkii a diferentes condições de degradação ambiental, através da condição corporal, índice hepatossomático e coloração do hepatopâncreas. A sobrevivência e resposta fisiológica de P. clarkii sujeito a condições de hipoxia foram avaliadas em condições laboratoriais. Adicionalmente analisaram-se indivíduos da mesma espécie, provenientes de diversos locais com diferentes condições ambientais. Verificou-se que condições de hipoxia moderada (3-5mg/L) não afetam a taxa de sobrevivência, o crescimento e a condição corporal e hepatossomática de P. clarkii. Indivíduos provenientes de massas de água com elevada degradação apresentam significativamente maior dimensão e condição corporal. O Índice hepatossomático assim como a cor do hepatopâncreas apresentam correlação com modificações hidro-morfológicas causadas por ação humana. Os resultados obtidos enfatizam a elevada tolerância de P. clarkii a condições ambientais de elevado constrangimento assim como demostram que esta espécie beneficia de condições ambientais degradadas; ABSTRACT: The Louisiana red crayfish, Procambarus clarkii (Girard 1852), is widely distributed throughout the world, being found in habitats with various levels of environmental quality. This study, aims to evaluate the physiological response of P. clarkii to environmental degradation through the body condition and hepatosomatic indexes and the color of the hepatopancreas. The survival and physiological response of P. clarkii under hypoxia was assessed in laboratory conditions. Additionally individuals of P. clarkii from several habitats with different environmental conditions were analyzed. It was found that conditions of moderate hypoxia (3-5mg / L) did not affect survival, growth and body and hepatossomatic condition of P. clarkii. Moreover, individuals from degraded water bodies presented significantly higher body size and condition the hepatosomatic index and the color of the hepatopancreas correlate with hydro-morphological changes caused by human action. This study demonstrates and emphasizes the great adaptability of this species to a wide range of environmental conditions as well as a high resistance to habitat degradation constrains

Seminário de Dissertação (2024)

Página da disciplina de Seminário de Dissertação (MPPP, UFPE, 2022) Lista de participantes == https://docs.google.com/spreadsheets/d/1mrULe1y04yPxHUBaF50jhaM1OY8QYJ3zva4N4yvm198/edit#gid=

Delayed colorectal cancer care during covid-19 pandemic (decor-19). Global perspective from an international survey

Background The widespread nature of coronavirus disease 2019 (COVID-19) has been unprecedented. We sought to analyze its global impact with a survey on colorectal cancer (CRC) care during the pandemic. Methods The impact of COVID-19 on preoperative assessment, elective surgery, and postoperative management of CRC patients was explored by a 35-item survey, which was distributed worldwide to members of surgical societies with an interest in CRC care. Respondents were divided into two comparator groups: 1) ‘delay’ group: CRC care affected by the pandemic; 2) ‘no delay’ group: unaltered CRC practice. Results A total of 1,051 respondents from 84 countries completed the survey. No substantial differences in demographics were found between the ‘delay’ (745, 70.9%) and ‘no delay’ (306, 29.1%) groups. Suspension of multidisciplinary team meetings, staff members quarantined or relocated to COVID-19 units, units fully dedicated to COVID-19 care, personal protective equipment not readily available were factors significantly associated to delays in endoscopy, radiology, surgery, histopathology and prolonged chemoradiation therapy-to-surgery intervals. In the ‘delay’ group, 48.9% of respondents reported a change in the initial surgical plan and 26.3% reported a shift from elective to urgent operations. Recovery of CRC care was associated with the status of the outbreak. Practicing in COVID-free units, no change in operative slots and staff members not relocated to COVID-19 units were statistically associated with unaltered CRC care in the ‘no delay’ group, while the geographical distribution was not. Conclusions Global changes in diagnostic and therapeutic CRC practices were evident. Changes were associated with differences in health-care delivery systems, hospital’s preparedness, resources availability, and local COVID-19 prevalence rather than geographical factors. Strategic planning is required to optimize CRC care

Characterisation of microbial attack on archaeological bone

As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones. © 2003 Elsevier Science Ltd. All rights reserved

Transverse momentum spectra of charged particles in proton–proton collisions at √s=900 GeV with ALICE at the LHC

The inclusive charged particle transverse momentum distribution is measured in proton–proton collisions at s=900 GeV at the LHC using the ALICE detector. The measurement is performed in the central pseudorapidity region (|η|<0.8) over the transverse momentum range 0.15<pT<10 GeV/c. The correlation between transverse momentum and particle multiplicity is also studied. Results are presented for inelastic (INEL) and non-single-diffractive (NSD) events. The average transverse momentum for |η|<0.8 is 〈pT〉INEL=0.483±0.001 (stat.)±0.007 (syst.) GeV/c and 〈pT〉NSD=0.489±0.001 (stat.)±0.007 (syst.) GeV/c, respectively. The data exhibit a slightly larger 〈pT〉 than measurements in wider pseudorapidity intervals. The results are compared to simulations with the Monte Carlo event generators PYTHIA and PHOJET

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field