TRY plant trait database - enhanced coverage and open access

Plant traits-the morphological, anatomical, physiological, biochemical and phenological characteristics of plants-determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait-based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits-almost complete coverage for 'plant growth form'. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait-environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives

Height and timing of growth spurt during puberty in young people living with vertically acquired HIV in Europe and Thailand.

OBJECTIVE: The aim of this study was to describe growth during puberty in young people with vertically acquired HIV. DESIGN: Pooled data from 12 paediatric HIV cohorts in Europe and Thailand. METHODS: One thousand and ninety-four children initiating a nonnucleoside reverse transcriptase inhibitor or boosted protease inhibitor based regimen aged 1-10 years were included. Super Imposition by Translation And Rotation (SITAR) models described growth from age 8 years using three parameters (average height, timing and shape of the growth spurt), dependent on age and height-for-age z-score (HAZ) (WHO references) at antiretroviral therapy (ART) initiation. Multivariate regression explored characteristics associated with these three parameters. RESULTS: At ART initiation, median age and HAZ was 6.4 [interquartile range (IQR): 2.8, 9.0] years and -1.2 (IQR: -2.3 to -0.2), respectively. Median follow-up was 9.1 (IQR: 6.9, 11.4) years. In girls, older age and lower HAZ at ART initiation were independently associated with a growth spurt which occurred 0.41 (95% confidence interval 0.20-0.62) years later in children starting ART age 6 to 10 years compared with 1 to 2 years and 1.50 (1.21-1.78) years later in those starting with HAZ less than -3 compared with HAZ at least -1. Later growth spurts in girls resulted in continued height growth into later adolescence. In boys starting ART with HAZ less than -1, growth spurts were later in children starting ART in the oldest age group, but for HAZ at least -1, there was no association with age. Girls and boys who initiated ART with HAZ at least -1 maintained a similar height to the WHO reference mean. CONCLUSION: Stunting at ART initiation was associated with later growth spurts in girls. Children with HAZ at least -1 at ART initiation grew in height at the level expected in HIV negative children of a comparable age

Relationship between functional fitness, medication costs and mood in elderly people

Objective: to verify if functional fitness (FF) is associated with the annual cost of medication consumption and mood states (MSt) in elderly people. Methods: a cross-sectional study with 229 elderly people aged 65 years or more at Santa Casa de Misericórdia de Coimbra, Portugal. Seniors with physical and psychological limitations were excluded, as well as those using medication that limits performance on the tests. The Senior Fitness Test was used to evaluate FF, and the Profile of Mood States - Short Form to evaluate the MSt. The statistical analysis was based on Mancova, with adjustment for age, for comparison between men and women, and adjustment for sex, for comparison between cardiorespiratory fitness quintiles. The association between the variables under study was made with partial correlation, controlling for the effects of age, sex and body mass index. Results: an inverse correlation between cardiorespiratory fitness and the annual cost of medication consumption was found (p < 0.01). FF is also inversely associated with MSt (p < 0.05). Comparisons between cardiorespiratory fitness quintiles showed higher medication consumption costs in seniors with lower aerobic endurance, as well as higher deterioration in MSt (p < 0.01). Conclusion: elderly people with better FF and, specifically, better cardiorespiratory fitness present lower medication consumption costs and a more positive MSt

Detectability of shape deformation in short-period exoplanets

Context Short-period planets are influenced by the extreme tidal forces of their parent stars. These forces deform the planets causing them to attain nonspherical shapes. The nonspherical shapes, modeled here as triaxial ellipsoids, can have an impact on the observed transit light-curves and the parameters derived for these planets. Aims We investigate the detectability of tidal deformation in short-period planets from their transit light curves and the instrumental precision needed. We also aim to show how detecting planet deformation allows us to obtain an observational estimate of the second fluid Love number from the light curve, which provides valuable information about the internal structure of the planet. Methods We adopted a model to calculate the shape of a planet due to the external potentials acting on it and used this model to modify the ellc transit tool. We used the modified ellc to generate the transit light curve for a deformed planet. Our model is parameterized by the Love number; therefore, for a given light curve we can derive the value of the Love number that best matches the observations. Results We simulated the known cases of WASP-103b and WASP-121b which are expected to be highly deformed. Our analyses show that instrumental precision ≤50 ppm min−1 is required to reliably estimate the Love number and detect tidal deformation. This precision can be achieved for WASP-103b in ∼40 transits using the Hubble Space Telescope and in ∼300 transits using the forthcoming CHEOPS instrument. However, fewer transits will be required for short-period planets that may be found around bright stars in the TESS and PLATO survey missions. The unprecedented precisions expected from PLATO and JWST will permit the detection of shape deformation with a single transit observation. However, the effects of instrumental and astrophysical noise must be considered as they can increase the number of transits required to reach the 50 ppm min−1 detection limit. We also show that improper modeling of limb darkening can act to bury signals related to the shape of the planet, thereby leading us to infer sphericity for a deformed planet. Accurate determination of the limb darkening coefficients is therefore required to confirm planet deformation

Quantitative RT-PCR analysis of differentially expressed genes in Quercus suber in response to Phytophthora cinnamomi infection

cDNA-AFLP methodology was used to gain insight into gene fragments differentially present in the mRNA profiles of Quercus suber roots infected with zoospores of Phytophthora cinnamomi at different post challenge time points. Fifty-three transcript-derived fragments (TDFs) were identified and sequenced. Six candidate genes were selected based on their expression patterns and homology to genes known to play a role in defence. They encode a cinnamyl alcohol dehydrogenase2 (QsCAD2), a protein disulphide isomerase (QsPDI), a CC-NBS-LRR resistance protein (QsRPc), a thaumatin-like protein (QsTLP), a chitinase (QsCHI) and a 1,3-β-glucanase (QsGlu). Evaluation of the expression of these genes by quantitative polymerase chain reaction (qPCR) revealed that transcript levels of QsRPc, QsCHI, QsCAD2 and QsPDI increased during the first 24 h post-inoculation, while those of thaumatin-like protein decreased. No differential expression was observed for 1,3-β-glucanase (QsGlu).Four candidate reference genes, polymerase II (QsRPII), eukaryotic translation initiation factor 5A (QsEIF-5A), β-tubulin (QsTUB) and a medium subunit family protein of clathrin adaptor complexes (QsCACs) were assessed to determine the most stable internal references for qRT-PCR normalization in the Phytophthora-Q. suber pathosystem in root tissues. Those found to be more stable, QsRPII and QsCACs, were used as internal reference in the present work.Knowledge on the Quercus defence mechanisms against biotic stress is scarce. This study provides an insight into the gene profiling of a few important genes of Q. suber in response to P. cinnamomi infection contributing to the knowledge of the molecular interactions involving Quercus and root pathogens that can be useful in the future to understand the mechanisms underlying oak resistance to soil-borne oomycetes.Peer Reviewe

Novel colorimetric-electrochemical methods for selective identification and quantification of Scopolamine in forensic analysis using screen-printed graphite electrodes and Dragendorff reagent

In forensic investigations, the detection of Scopolamine, popularly known as Burundanga or Devil's Breath, is of significant interest due to its potential involvement in cases of attempted murder or suicide. Currently, no efficient screening methods exist for Scopolamine detection in such forensic contexts. This study presents a novel method combining screen-printed graphite electrodes (SPGE) with square-wave voltammetry (electrochemical step) and Dragendorff reagent (colorimetric step) to detect Scopolamine in drinks (gin, tonic water, whisky, and energy drinks) and biological samples (urine, saliva, and vitreous humor). The method provides two distinct analytical responses: a visible color change (from orange to yellow) via the colorimetric reaction, and the electrochemical behavior of Scopolamine in both anodic and cathodic scans, ensuring robust and accurate identification. For the first time, the electrochemical behavior of both redox processes of Scopolamine is investigated. The proposed method demonstrated a wide linear range (0.025-0.225 mg mL−1 for the oxidation and 0.025–0.175 mg mL⁻¹ for the reduction process) with a low limit of detection of 5.0 μg mL−1, making it suitable for forensic applications. Stability of the electrochemical response was studied with SPGE showing relative standard deviations (RSD) of less than 3 % for Ep and Ip across multiple electrodes (N = 3). Interference studies confirmed the method's high selectivity for Scopolamine detection. Additionally, Scopolamine was successfully identified in both beverage and biological samples with recoveries near 100 %, indicating the absence of matrix effects. The methodology using both electrochemical with a colorimetric approach presents a promising, rapid, and selective screening method for Scopolamine detection in forensic scenarios

Most microRNAs in the single-cell alga Chlamydomonas reinhardtii are produced by Dicer-like 3-mediated cleavage of introns and untranslated regions of coding RNAs.

We describe here a forward genetic screen to investigate the biogenesis, mode of action, and biological function of miRNA-mediated RNA silencing in the model algal species,Chlamydomonas reinhardtii Among the mutants from this screen, there were three at Dicer-like 3 that failed to produce both miRNAs and siRNAs and others affecting diverse post-biogenesis stages of miRNA-mediated silencing. The DCL3-dependent siRNAs fell into several classes including transposon- and repeat-derived siRNAs as in higher plants. The DCL3-dependent miRNAs differ from those of higher plants, however, in that many of them are derived from mRNAs or from the introns of pre-mRNAs. Transcriptome analysis of the wild-type and dcl3 mutant strains revealed a further difference from higher plants in that the sRNAs are rarely negative switches of mRNA accumulation. The few transcripts that were more abundant in dcl3 mutant strains than in wild-type cells were not due to sRNA-targeted RNA degradation but to direct DCL3 cleavage of miRNA and siRNA precursor structures embedded in the untranslated (and translated) regions of the mRNAs. Our analysis reveals that the miRNA-mediated RNA silencing in C. reinhardtii differs from that of higher plants and informs about the evolution and function of this pathway in eukaryotes.Work in the Baulcombe laboratory is supported by the Balzan Prize award and the ERC Advanced Investigator Grant ERC-2013-AdG 340642 TRIBE. AAV was supported by a Marie-Curie fellowship (PIEF-GA-2010-276037). BYC was supported by an EMBL long-term postdoctoral fellowship and a Sir Henry Wellcome Fellowship (096082). DCB is the Royal Society Edward Penley Abraham Research Professor.This is the final version of the article. It first appeared from Cold Spring Harbor Laboratory Press via https://doi.org/10.1101/gr.199703.11

Cuidadores informais de idosos em pós-operatório de cirurgia de fêmur proximal: prevenção de novas quedas

The objectives of this study were to investigate the sociodemographic characteristics of informal caregivers of elderly persons who had undergone surgery for hip fractures caused by a fall, explore the level of caregiver's knowledge regarding fall prevention, and assess the relationship between this knowledge and the use of preventative measures in practice. This investigation consists of a cross-sectional study using nonprobability sampling methods conducted over a period of 12 months and involving 89 caregivers. The majority of caregivers were female (76.4%) and sons or daughters of the patients (64%). Environmental modification was the predominant preventative measure used by caregivers (88.2%). 58.1% of caregivers believed it was possible to prevent falls in the elderly and there was a significant association (p = 0,002) between believing it was possible to prevent falls and carrying out modifications in the home and/or to the daily routine of the older person. Informal caregivers with wide or partial knowledge of fall prevention put preventative measures into practice. These findings demonstrate that the number of falls among older persons could be significantly reduced if health care programmes widened their actions to include the guiding principles of the WHO falls prevention model.Os objetivos foram caracterizar sociodemograficamente os cuidadores informais de idosos vítimas de queda seguida por fratura de fêmur proximal e verificar o conhecimento mínimo que possuíam acerca da prevenção de novas quedas, assim como caracterizar a relação entre esse conhecimento e o emprego de medidas preventivas. Trata-se de estudo transversal, com amostragem intencional, realizado em 12 meses, incluindo 89 cuidadores. Predominaram cuidadores do sexo feminino (76,4%) e filhas(os) (64%). A modificação ambiental foi a medida preventiva predominante apontada por eles (88,2%). Houve associação significativa (p = 0,002), entre os 58,1% dos cuidadores que achavam ser possível prevenir quedas e os relatos sobre mudanças na casa e/ou rotina do idoso. Observou-se que cuidadores informais que apresentavam conhecimento sobre prevenção de quedas em idosos, mesmo que incompletos, empregavam medidas de prevenção para novos eventos. Esses achados sinalizam que o número de quedas entre idosos pode ser reduzido significantemente se os programas de atenção à saúde ampliarem suas ações apoiando-as no modelo de prevenção de quedas da Organização Mundial de Saúde.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista (Unesp), Departamento de Enfermagem, Faculdade de Medicina de Botucatu (FMB), Distrito de Rubião Júnior snº, Centro. 18618-970, Botucatu SP BrasilUniversidade Estadual Paulista (Unesp), Departamento de Cirurgia e Ortopedia, FBM, Unesp.Universidade Estadual Paulista (Unesp), Departamento de Enfermagem, Faculdade de Medicina de Botucatu (FMB), Distrito de Rubião Júnior snº, Centro. 18618-970, Botucatu SP BrasilUniversidade Estadual Paulista (Unesp), Departamento de Cirurgia e Ortopedia, FBM, Unesp

Quality of life of caregivers for patients of cerebrovascular accidents: association of (socio-demographic) characteristics and burden

OBJECTIVE Investigating the association between quality of life with socio-demographic characteristics and the burden of caregivers for individuals with cerebrovascular accident sequelae. METHOD A descriptive, cross-sectional study with a sample composed of 136 caregivers. For data collection, a semi-structured questionnaire, the Barthel, Burden Interview and Short-Form-36 scales were used. Correlation analysis, t-Student test and F-test were used for the analysis in order to compare averages. RESULTS Significant averages in quality of life were demonstrated in association with female caregivers and those over 60 years in the field 'functional capacity,' and in the domains of 'mental health' and 'vitality' for those with higher income. Regarding burden association, the highlighted areas were 'functional capacity,' 'physical aspects,' 'emotional aspects' and 'pain.' CONCLUSION The creation of public policies and social support to effectively reduce the burden on caregivers is a necessity