Persistent activation of NF-kappaB related to IkappaB's degradation profiles during early chemical hepatocarcinogenesis

BACKGROUND: To define the NF-kappaB activation in early stages of hepatocarcinogenesis and its IkappaB's degradation profiles in comparison to sole liver regeneration. METHODS: Western-blot and EMSA analyses were performed for the NF-kappaB activation. The transcriptional activity of NF-kappaB was determined by RT-PCR of the IkappaB-α mRNA. The IkappaB's degradation proteins were determined by Western-blot assay. RESULTS: We demonstrated the persistent activation of NF-kappaB during early stages of hepatocarcinogenesis, which reached maximal level 30 min after partial hepatectomy. The DNA binding and transcriptional activity of NF-kappaB, were sustained during early steps of hepatocarcinogenesis in comparison to only partial hepatectomy, which displayed a transitory NF-kappaB activation. In early stages of hepatocarconogenesis, the IkappaB-α degradation turned out to be acute and transitory, but the low levels of IkappaB-β persisted even 15 days after partial hepatectomy. Interestingly, IkappaB-β degradation is not induced after sole partial hepatectomy. CONCLUSION: We propose that during liver regeneration, the transitory stimulation of the transcription factor response, assures blockade of NF-kappaB until recovery of the total mass of the liver and the persistent NF-kappaB activation in early hepatocarcinogenesis may be due to IkappaB-β and IkappaB-α degradation, mainly IkappaB-β degradation, which contributes to gene transcription related to proliferation required for neoplasic progression

Cytotoxicity properties of PM2.5 collected in Wrocław agglomeration

WHO report published in May 2016 presents the cities with the highest concentrations of particulate matter fraction 10 μm and 2.5 μm on the earth. Report presents that many of Polish cities are on the top of polluted regions in European Union. One of the cities mentioned in the report is Wrocław. Wrocław is the urban and industrial agglomeration, where air pollutions comes from three main sources: low-emissions, industrial emissions and urban traffic. In recent years, reduction in annual average concentrations of particulate matter has been observed. However Wrocław is still one of the cities in Poland, which have problems with smog situations during winter season. This article presents results of researches conducted on the particulate matter fraction 2.5 μm collected in Wrocław agglomeration. Pollutions have been tested using PAN-I assay kit, which contains 4 single toxicity tests allows to check different parameters defining toxic effects on human cells. Cytotoxicity tests which are conducted are: LDH test to evaluate the integrity of cell membrane, XTT test to assess mitochondrial activity, NR test for the evaluation of the lysosomal activity and SRB test to evaluate the total protein content. Tests were performed on adenocarcinomic human alveolar basal epithelial cell line (A549), which allows to simulate conditions as close as real

Celecoxib enhances the detoxification of diethylnitrosamine in rat liver cancer

AIM: To study the effect of celecoxib (CXB) on diethylnitrosamine activation through the regulation of cytochrome P450 in a hepatocarcinogenesis model

Celecoxib activates Stat5 and restores or increases the expression of growth hormone-regulated genes in hepatocarcinogenesis

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