Butyrate Esterase (Tributyrin) Spot Test, a Simple Method for Immediate Identification of Moraxella (Branhamella) catarrhalis

A total of 47 Moraxella (Branhamella) catarrhalis strains, 89 strains of Neisseria spp., and 82 strains of miscellaneous bacteria and yeasts were studied by using a fluorogenic spot method which detects butyrate esterase. A positive butyrate esterase spot test correctly differentiated M. catarrhalis from Neisseria spp., which had a negative butyrate esterase spot test reaction. The test is rapid, simple, and easy to perform. The butyrate esterase spot test was useful for direct identification of M. catarrhalis from primary cultures. However, false-positive reactions may occur with mixed cultures.Facultad de Ciencias Veterinaria

Butyrate Esterase (Tributyrin) Spot Test, a Simple Method for Immediate Identification of Moraxella (Branhamella) catarrhalis

A total of 47 Moraxella (Branhamella) catarrhalis strains, 89 strains of Neisseria spp., and 82 strains of miscellaneous bacteria and yeasts were studied by using a fluorogenic spot method which detects butyrate esterase. A positive butyrate esterase spot test correctly differentiated M. catarrhalis from Neisseria spp., which had a negative butyrate esterase spot test reaction. The test is rapid, simple, and easy to perform. The butyrate esterase spot test was useful for direct identification of M. catarrhalis from primary cultures. However, false-positive reactions may occur with mixed cultures.Facultad de Ciencias Veterinaria

Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry

La propagación vegetativa del cultivo de frutilla favorece la transmisión de patógenos sistémicos, como es el caso de los virus, que constituyen uno de los principales factores limitantes. Se han descripto más de 20 virus que infectan esta especie; el Strawberry mottle virus (SMoV) es uno de los más frecuentes y responsable de importantes pérdidas económicas. Debido a la falta de antisuero disponible comercialmente para un diagnóstico serológico, el SMoV es detectado fundamentalmente mediante reacción en cadena de la polimerasa con transcripción reversa (RT-PCR). En este estudio se desarrolló una sonda de hibridación molecular no radioactiva para su detección. Se sintetizó cDNA con cebadores específicos diseñados a partir de la región 3’ no codificante del genoma viral. El cDNA obtenido fue clonado, marcado y utilizado como sonda. Se evaluaron seis protocolos de extracción de ARN viral a partir de plantas infectadas, de los cuales el método de bromuro de cetiltrimetilamonio modificado (CTAB) fue el más eficiente. Se evaluaron hojas de diferentes estados fenológicos y pecíolos, y fueron las hojas viejas y los pecíolos los que mostraron mayor reacción.The vegetative propagation of strawberries favors transmission of systemicpathogens, such as viruses, which are one of the main yield-limiting factorsfor this crop. More than 20 viruses have been described as infecting thisspecies; one of the most frequent is the Strawberry mottle virus (SMoV), whichis responsible for significant economic losses. SMoV is usually detected byreverse transcription polymerase chain reaction (RT-PCR), given that serum isnot available for serological tests. In this study, a non-radioactive molecularprobe was developed for SMoV detection. The cDNA was synthesized byRT-PCR using specific primers designed from the 3?UTR region of the viralgenome. The cloned cDNA segment was labeled and used as a probe. Six RNAextraction protocols were evaluated, and the modified cetyltrimethylammoniumbromide (CTAB) method showed the highest sensitivity level. Leaves at differentphenological stages and petioles were evaluated; the highest reaction wasobserved in old leaves and in petioles.Fil: Asinari, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Cafrune, Eva Encarnacion. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Guzman, F. A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentin

‘Ca. Phytoplasma pruni’ and ‘Ca. Phytoplasma meliae’ are affecting plum in Argentina

We report for the first time the presence of phytoplasmas in association with Plum (Prunus domestica) yellowing in Argentina. Molecular analysis of 16S rDNA gene sequence (RFLP, phylogeny) reveals the existence of two different 16Sr-subgroups, 16SrIII-B and novel 16SrXIII-L. These results contribute to a better understanding of the diversity of phytoplasmas associated with Prunus genera in South America.Instituto de Patología VegetalFil: Bongiorno, Vanina Aylén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bongiorno, Vanina Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); ArgentinaFil: Alessio, Florencia Ivette. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Alessio, Florencia Ivette. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); ArgentinaFil: Curzel, Viviana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta. Agencia De Extensión Rural Perico; ArgentinaFil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina.Fil: Nome Docampo, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); ArgentinaFil: Fernandez, Franco Daniel . Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina.Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas.Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina

The interview in the context of research and the F.I.S

Este trabajo se inscribe en el marco de la Investigación denominada “Actualización del Test Frases Incompletas de Joseph M. Sacks”, que se realiza en la Facultad de Psicología de la U.N.L.P. En esta oportunidad abordaremos la Entrevista dentro del quehacer cualitativo como una herramienta eficaz para desentrañar significaciones elaboradas por los sujetos mediante sus discursos, relatos y experiencias. Uno de los inconvenientes que presenta este instrumento es en el proceso previo a la aplicación de la misma, momento en que se requiere unificar criterios en la construcción metodológica del dato, para poder ser aplicada por uno o más investigadores, sin alterar los objetivos del estudio. Resulta clave, por lo tanto, reflexionar en cómo elaborar una entrevista de manera correcta, sobre todo su guión, lo cual incluye las preguntas pertinentes al objeto de estudio, que el entrevistador va a usar. La consecuencia esperada es la obtención de contenidos veraces por el proceso en sí y la dinámica que permitirá la correlación con las respuestas obtenidas en las 60 frases incompletas que el sujeto debe completar contestando lo primero que se le ocurra.This work is part of the research called “Update of the Joseph M. Sacks Incomplete Phrases Test”, which is carried out at the Faculty of Psychology of the U.N.L.P. In this opportunity we will approach the Interview within the qualitative task as an effective tool to unravel meanings elaborated by the subjects through their speeches, stories and experiences. One of the drawbacks of this instrument is in the process prior to its application, at which time it is required to unify criteria in the methodological construction of the data, in order to be applied by one or more researchers, without altering the objectives of the study. It is key, therefore, to reflect on how to prepare an interview correctly, especially its script, which includes the pertinent questions to the object of study, which the interviewer is going to use. The expected consequence is the obtaining of truthful content through the process itself and the dynamics that will allow the correlation with the answers obtained in the 60 incomplete sentences that the subject must complete by answering the first thing that comes to mind.Facultad de Psicologí

Measurement of t(t)over-bar normalised multi-differential cross sections in pp collisions at root s=13 TeV, and simultaneous determination of the strong coupling strength, top quark pole mass, and parton distribution functions

Peer reviewe

MUSiC : a model-unspecific search for new physics in proton-proton collisions at root s=13TeV

Results of the Model Unspecific Search in CMS (MUSiC), using proton-proton collision data recorded at the LHC at a centre-of-mass energy of 13 TeV, corresponding to an integrated luminosity of 35.9 fb(-1), are presented. The MUSiC analysis searches for anomalies that could be signatures of physics beyond the standard model. The analysis is based on the comparison of observed data with the standard model prediction, as determined from simulation, in several hundred final states and multiple kinematic distributions. Events containing at least one electron or muon are classified based on their final state topology, and an automated search algorithm surveys the observed data for deviations from the prediction. The sensitivity of the search is validated using multiple methods. No significant deviations from the predictions have been observed. For a wide range of final state topologies, agreement is found between the data and the standard model simulation. This analysis complements dedicated search analyses by significantly expanding the range of final states covered using a model independent approach with the largest data set to date to probe phase space regions beyond the reach of previous general searches.Peer reviewe

Measurement of prompt open-charm production cross sections in proton-proton collisions at root s=13 TeV

The production cross sections for prompt open-charm mesons in proton-proton collisions at a center-of-mass energy of 13TeV are reported. The measurement is performed using a data sample collected by the CMS experiment corresponding to an integrated luminosity of 29 nb(-1). The differential production cross sections of the D*(+/-), D-+/-, and D-0 ((D) over bar (0)) mesons are presented in ranges of transverse momentum and pseudorapidity 4 < p(T) < 100 GeV and vertical bar eta vertical bar < 2.1, respectively. The results are compared to several theoretical calculations and to previous measurements.Peer reviewe

Search for new particles in events with energetic jets and large missing transverse momentum in proton-proton collisions at root s=13 TeV

A search is presented for new particles produced at the LHC in proton-proton collisions at root s = 13 TeV, using events with energetic jets and large missing transverse momentum. The analysis is based on a data sample corresponding to an integrated luminosity of 101 fb(-1), collected in 2017-2018 with the CMS detector. Machine learning techniques are used to define separate categories for events with narrow jets from initial-state radiation and events with large-radius jets consistent with a hadronic decay of a W or Z boson. A statistical combination is made with an earlier search based on a data sample of 36 fb(-1), collected in 2016. No significant excess of events is observed with respect to the standard model background expectation determined from control samples in data. The results are interpreted in terms of limits on the branching fraction of an invisible decay of the Higgs boson, as well as constraints on simplified models of dark matter, on first-generation scalar leptoquarks decaying to quarks and neutrinos, and on models with large extra dimensions. Several of the new limits, specifically for spin-1 dark matter mediators, pseudoscalar mediators, colored mediators, and leptoquarks, are the most restrictive to date.Peer reviewe

Measurement of the top quark forward-backward production asymmetry and the anomalous chromoelectric and chromomagnetic moments in pp collisions at √s = 13 TeV

Abstract The parton-level top quark (t) forward-backward asymmetry and the anomalous chromoelectric (d̂ t) and chromomagnetic (μ̂ t) moments have been measured using LHC pp collisions at a center-of-mass energy of 13 TeV, collected in the CMS detector in a data sample corresponding to an integrated luminosity of 35.9 fb−1. The linearized variable AFB(1) is used to approximate the asymmetry. Candidate t t ¯ events decaying to a muon or electron and jets in final states with low and high Lorentz boosts are selected and reconstructed using a fit of the kinematic distributions of the decay products to those expected for t t ¯ final states. The values found for the parameters are AFB(1)=0.048−0.087+0.095(stat)−0.029+0.020(syst),μ̂t=−0.024−0.009+0.013(stat)−0.011+0.016(syst), and a limit is placed on the magnitude of | d̂ t| &lt; 0.03 at 95% confidence level. [Figure not available: see fulltext.