Quenching of 1+1^+ excitations in the double giant resonance

The electromagnetic excitation of the two-phonon isovector giant dipole resonance in relativistic projectiles incident on heavy targets can be proceed via several intermediate $1^-$ one-phonon giant resonance states. In two step electric dipole transitions the population of $0^+$, $1^+$, and $2^+$ two-phonon states are possible. We calculate the amplitude distribution of $1^-$ excitations with an RPA formalism, and use it to calculate the electromagnetic excitation of two-phonon states in second order perturbation theory and coupled-channels. We show that a conspiracy between angular momentum coupling and the strength of the electromagnetic fields suppresses contributions of $1^+$ states to the total cross sections.Comment: 10 pages, 2 Postscript figures available upon reques

How to us Nile Red, a selective fluorescent stain for microalgal neutral lipids

The use of Nile Red for rapid monitoring of the neutral lipid content in microalgae has gained interest over the last decade, since neutral lipids are feedstock for renewable transportation fuel. In this review, we discuss the main considerations needed to make an NR protocol reliable for staining neutral lipids in microalgae. Cell wall permeability must be enhanced by using stain carriers: DMSO (5% v/v to 25% v/v), glycerol (0.1 to 0.125 mg/mL), or EDTA (3.0 to 3.8 mg/mL). Temperatures between 30 and 40 °C facilitate the diffusion of NR through the cell wall without incurring excess quenching. Good NR-lipid interaction requires using a low NR/cell ratio; the NR concentration must be between 0.25 μg/mL and 2.0 μg/mL, and the cell concentration > 5 × 104 cells/mL. In order to have the maximum and stable NR fluorescence, it is necessary to scan the excitation/emission wavelengths for up to a 40-min of incubation time. We outline a five-step method to customize the Nile Red protocol to a specific strain: 1) Evaluate the strain's suitability by checking for the presence of neutral lipid, 2) Select of the best excitation/emission wavelength, 3) Optimization of incubation time, stain carrier, dye concentration, and temperature, 4) Prepare single-strain algal cultures with different lipid contents to calibrate NR fluorescence with neutral-lipid content, and 5) Correlate NR fluorescence intensity to neutral lipid content for the same strain. Once the protocol is customized, the NR method allows for rapid and reliable monitoring of neutral lipid content of a microalgae strain.</p