27 research outputs found
The participation of plant cell organelles in compatible and incompatible potato virus Y-tobacco and -potato plant interaction
Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants
Multicolor Fluorescence Imaging of Leaves-A Useful Tool for Visualizing Systemic Viral Infections in Plants â€
Robotized Thermal and Chlorophyll Fluorescence Imaging of Pepper Mild Mottle Virus Infection in Nicotiana benthamiana
PsbR, a Missing Link in the Assembly of the Oxygen-evolving Complex of Plant Photosystem II
Expression of the CMV-CP Gene in Synechocystis
Previous work has shown that the presence of excess coat protein (CP) of cucumber mosaic virus (CMV) in the chloroplasts was related with mosaic symptoms. However, whether these mosaic symptoms are directly induced by the interaction between CP and chloroplasts is unknown. To directly demonstrate the interaction between CP and the chloroplast, Synechocystis sp. PCC 6803 was used as the chloroplast model. The cDNA encoding the CMV-CP was cloned in a cyanobacterial shuttle vector (pKT-CP) and transferred to Synechocystis sp. PCC 6803. The CP was expressed in the cyanobacterium with the psbA promoter. The expression of CMV-CP hindered the growth of transgenic cyanobacterium cells and decreased its photosynthetic rate and the PS II activity. The transgenic cells showed increased fluorescence (F) from the phycobilisome terminal emitters and increased fluorescence (F) from PS II. The absorption spectra at room temperature showed the Chl and the phycocyanin absorption peak of the mutant strain significantly decreased. These results showed that CP may directly affect the cyanobacterium cells and decreased its photosynthesis, especially the PS II activity. These data might provide new evidence for mosaic symptoms being directly induced by the interaction between CP and chloroplasts