Decreased sexual risk behaviour after the diagnosis of HIV and initiation of antiretroviral treatment - a study of patients in Johannesburg

Objectives. An extended programme for free antiretroviral treatment (ART) of HIV was launched in South Africa in April 2004. It is essential to assess the effects on sexual risk behaviour. Design and setting. A questionnaire was distributed to patients on ART at Helen Joseph Hospital, Johannesburg, between 17 January and 22 February 2005. Results. The percentage of men who had sexual contacts outside their relationship decreased from 48% before HIV diagnosis to 11% after starting ART. Condom use with casual partners increased from 53% among the men and 46% among the women before the diagnosis of HIV to 87% and 81% respectively on ART. The majority of patients were tested for HIV because they presented with symptoms of illness. We noted no significant difference in disclosure rate after the start of ART. All participants were positive about the treatment and felt physically better. The majority of the patients experienced a better quality of life. Conclusions. The ART had an overall positive effect on health with no increase of sexual risk behaviour. Southern African Journal of HIV Medicine Vol. 7 (4) 2006: pp. 12-1

Hedgehog signaling is necessary and sufficient to mediate craniofacial plasticity in teleosts

Phenotypic plasticity, the ability of a single genotype to produce multiple phenotypes under different environmental conditions, is critical for the origins and maintenance of biodiversity; however, the genetic mechanisms underlying plasticity as well as how variation in those mechanisms can drive evolutionary change remain poorly understood. Here, we examine the cichlid feeding apparatus, an icon of both prodigious evolutionary divergence and adaptive phenotypic plasticity. We first provide a tissue-level mechanism for plasticity in craniofacial shape by measuring rates of bone deposition within functionally salient elements of the feeding apparatus in fishes forced to employ alternate foraging modes. We show that levels and patterns of phenotypic plasticity are distinct among closely related cichlid species, underscoring the evolutionary potential of this trait. Next, we demonstrate that hedgehog (Hh) signaling, which has been implicated in the evolutionary divergence of cichlid feeding architecture, is associated with environmentally induced rates of bone deposition. Finally, to demonstrate that Hh levels are the cause of the plastic response and not simply the consequence of producing more bone, we use transgenic zebrafish in which Hh levels could be experimentally manipulated under different foraging conditions. Notably, we find that the ability to modulate bone deposition rates in different environments is dampened when Hh levels are reduced, whereas the sensitivity of bone deposition to different mechanical demands increases with elevated Hh levels. These data advance a mechanistic understanding of phenotypic plasticity in the teleost feeding apparatus and in doing so contribute key insights into the origins of adaptive morphological radiations

Eruptions at Lone Star Geyser, Yellowstone National Park, USA: 1. Energetics and eruption dynamics

Author Posting. © American Geophysical Union, 2013. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Solid Earth 118 (2013): 4048–4062, doi:10.1002/jgrb.50251.Geysers provide a natural laboratory to study multiphase eruptive processes. We present results from a 4 day experiment at Lone Star Geyser in Yellowstone National Park, USA. We simultaneously measured water discharge, acoustic emissions, infrared intensity, and visible and infrared video to quantify the energetics and dynamics of eruptions, occurring approximately every 3 h. We define four phases in the eruption cycle (1) a 28±3 min phase with liquid and steam fountaining, with maximum jet velocities of 16–28 m s−1, steam mass fraction of less than ∼0.01. Intermittently choked flow and flow oscillations with periods increasing from 20 to 40 s are coincident with a decrease in jet velocity and an increase of steam fraction; (2) a 26±8 min posteruption relaxation phase with no discharge from the vent, infrared (IR), and acoustic power oscillations gliding between 30 and 40 s; (3) a 59±13 min recharge period during which the geyser is quiescent and progressively refills, and (4) a 69±14 min preplay period characterized by a series of 5–10 min long pulses of steam, small volumes of liquid water discharge, and 50–70 s flow oscillations. The erupted waters ascend from a 160–170°C reservoir, and the volume discharged during the entire eruptive cycle is 20.8±4.1 m3. Assuming isentropic expansion, we calculate a heat output from the geyser of 1.4–1.5 MW, which is <0.1% of the total heat output from Yellowstone Caldera.Support comes from NSF (L. Karlstrom, M. Manga), the USGS Volcano Hazards program (S. Hurwitz, F. Murphy, M.J.S. Johnston, and R.B. McCleskey), and WHOI (R. Sohn).2014-02-1

Late Miocene erosion and evolution of topography along the western slope of the Colorado Rockies

In the Colorado Rocky Mountains, the association of high topography and low seismic velocity in the underlying mantle suggests that recent changes in lithospheric buoyancy may have been associated with surface uplift of the range. This paper examines the relationships among late Cenozoic fl uvial incision, channel steepness, and mantle velocity domains along the western slope of the northern Colorado Rockies. New 40Ar/39Ar ages on basalts capping the Tertiary Browns Park Formation range from ca. 11 to 6 Ma and provide markers from which we reconstruct incision along the White, Yampa, and Little Snake rivers. The magnitude of post-10 Ma incision varies systematically from north to south, increasing from ~ 500 m along the Little Snake River to ~1500 m along the Colorado River. Spatial variations in the amount of late Cenozoic incision are matched by metrics of channel steepness; the upper Colorado River and its tributaries (e.g., Gunnison and Dolores rivers) are two to three times steeper than the Yampa and White rivers, and these variations are independent of both discharge and lithologic substrate. The coincidence of steep river profi les with deep incision suggests that the fl uvial systems are dynamically adjusting to an external forcing but is not readily explained by a putative increase in erosivity associated with late Cenozoic climate change. Rather, channel steepness correlates with the position of the channels relative to low-velocity mantle. We suggest that the history of late Miocene-present incision and channel adjustment refl ects long-wavelength tilting across the western slope of the Rocky Mountains

The laurentian record of neoproterozoic glaciation, tectonism, and eukaryotic evolution in Death Vally, California

Neoproterozoic strata in Death Valley, California contain eukaryotic microfossils and glacial deposits that have been used to assess the severity of putative Snowball Earth events and the biological response to extreme environmental change. These successions also contain evidence for syn-sedimentary faulting that has been related to the rifting of Rodinia, and in turn the tectonic context of the onset of Snowball Earth. These interpretations hinge on local geological relationships and both regional and global stratigraphic correlations. Here we present new geological mapping, measured stratigraphic sections, carbon and strontium isotope chemostratigraphy, and micropaleontology from the Neoproterozoic glacial deposits and bounding strata in Death Valley. These new data enable us to refine regional correlations both across Death Valley and throughout Laurentia, and construct a new age model for glaciogenic strata and microfossil assemblages. Particularly, our remapping of the Kingston Peak Formation in the Saddle Peak Hills and near the type locality shows for the first time that glacial deposits of both the Marinoan and Sturtian glaciations can be distinguished in southeastern Death Valley, and that beds containing vase-shaped microfossils are slump blocks derived from the underlying strata. These slump blocks are associated with multiple overlapping unconformities that developed during syn-sedimentary faulting, which is a common feature of Cyrogenian strata along the margin of Laurentia from California to Alaska. With these data, we conclude that all of the microfossils that have been described to date in Neoproterozoic strata of Death Valley predate the glaciations and do not bear on the severity, extent or duration of Neoproterozoic Snowball Earth events

Abandonment of Unaweep Canyon (1.4-0.8 Ma), western Colorado: Effects of stream capture and anomalously rapid Pleistocene river incision

Cosmogenic-burial and U-series dating, identification of fluvial terraces and lacustrine deposits, and river profile reconstructions show that capture of the Gunnison River by the Colorado River and abandonment of Unaweep Canyon (Colorado, USA) occurred between 1.4 and 0.8 Ma. This event led to a rapid pulse of incision unlike any documented in the Rocky Mountains. Following abandonment of Unaweep Canyon by the ancestral Gunnison River, a wave of incision propagated upvalley rapidly through Mancos Shale at rates of ̃90-440 km/m.y. The Gunnison River removed 400-500 km3 of erodible Mancos Shale and incised as deep as 360 m in 0.17-0.76 m.y. (incision rates of ̃470-2250 m/m.y.). Prior to canyon abandonment, long-term (ca. 11-1 Ma) Gunnison River incision averaged ̃100 m/m.y. The wave of incision also caused the subsequent capture of the Bostwick-Shinn Park River by the ancestral Uncompahgre River ca. 0.87-0.64 Ma, at a location ̃70 km upvalley from Unaweep Canyon. This event led to similarly rapid (to ̃500 m/m.y.) but localized river incision. As regional river incision progressed, the juxtaposition of resistant Precambrian bedrock and erodible Mancos Shale within watersheds favored the development of significant relief between adjacent stream segments, which led to stream piracy. The response of rivers to the abandonment of Unaweep Canyon illustrates how the mode and tempo of long-term fluvial incision are punctuated by short-term geomorphic events such as stream piracy. These shortterm events can trigger significant landscape changes, but the effects are more localized relative to regional climatically or tectonically driven events

Feedback between deformation and magmatism in the Lloyds River Fault Zone : an example of episodic fault reactivation in an accretionary setting, Newfoundland Appalachians

Author Posting. © American Geophysical Union, 2006. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Tectonics 25 (2006): TC4004, doi:10.1029/2005TC001789.The Lloyds River Fault Zone is a 10–15 km wide amphibolite-grade shear zone that formed during the Ordovician Taconic Orogeny. It separates ophiolites and arc–back-arc complexes formed in Iapetus from a peri-Laurentian microcontinent (Dashwoods microcontinent). The Lloyds River Fault Zone comprises three high-strain zones, dominantly composed of mylonitic amphibolites, separated by less deformed plutonic rocks. Structural, age and metamorphic data suggest the Lloyds River Fault Zone accommodated sinistral-oblique underthrusting of ophiolites underneath the Dashwoods microcontinent prior to 471 ± 5 Ma at 800°C and 6 kbar. Plutonic rocks within the Lloyds River Fault Zone comprise two suites dated at 464 ± 2 plus 462 ± 2 and 459 ± 3 Ma, respectively. The younger age of the plutons with respect to some of the amphibolites, evidence for magmatic deformation, and the elongate nature of the plutons parallel to the Lloyds River Fault Zone suggest they were emplaced within the fault zone during deformation. Both intrusive episodes triggered renewed deformation at high temperatures (770–750°C), illustrating the positive feedback between deformation and magmatism. Offshoots of the plutons intruded undeformed ophiolitic gabbros outside the Lloyds River Fault Zone. Deformation localized within the intrusive sheets, coeval with static contact metamorphism of the host gabbros, leading to the development of new, small-scale shear zones. This illustrates that channeling of plutons into shear zones and nucleation of shear zones in melt-rich zones may occur simultaneously within the same fault system.This research is funded by a scholarship from the Faculty of Graduate and Postdoctoral Studies, University of Ottawa, to C.J.L. and a NSERC grant to C.v.S in his position as Adjunct Professor at the University of Ottawa

Attenuation of Notch and Hedgehog Signaling Is Required for Fate Specification in the Spinal Cord

During the development of the spinal cord, proliferative neural progenitors differentiate into postmitotic neurons with distinct fates. How cells switch from progenitor states to differentiated fates is poorly understood. To address this question, we studied the differentiation of progenitors in the zebrafish spinal cord, focusing on the differentiation of Kolmer-Agduhr″ (KA″) interneurons from lateral floor plate (LFP) progenitors. In vivo cell tracking demonstrates that KA″ cells are generated from LFP progenitors by both symmetric and asymmetric cell divisions. A photoconvertible reporter of signaling history (PHRESH) reveals distinct temporal profiles of Hh response: LFP progenitors continuously respond to Hh, while KA″ cells lose Hh response upon differentiation. Hh signaling is required in LFP progenitors for KA″ fate specification, but prolonged Hh signaling interferes with KA″ differentiation. Notch signaling acts permissively to maintain LFP progenitor cells: activation of Notch signaling prevents differentiation, whereas inhibition of Notch signaling results in differentiation of ectopic KA″ cells. These results indicate that neural progenitors depend on Notch signaling to maintain Hh responsiveness and rely on Hh signaling to induce fate identity, whereas proper differentiation depends on the attenuation of both Notch and Hh signaling

The Genomics of Disulfide Bonding and Protein Stabilization in Thermophiles

Thermophilic organisms flourish in varied high-temperature environmental niches that are deadly to other organisms. Recently, genomic evidence has implicated a critical role for disulfide bonds in the structural stabilization of intracellular proteins from certain of these organisms, contrary to the conventional view that structural disulfide bonds are exclusively extracellular. Here both computational and structural data are presented to explore the occurrence of disulfide bonds as a protein-stabilization method across many thermophilic prokaryotes. Based on computational studies, disulfide-bond richness is found to be widespread, with thermophiles containing the highest levels. Interestingly, only a distinct subset of thermophiles exhibit this property. A computational search for proteins matching this target phylogenetic profile singles out a specific protein, known as protein disulfide oxidoreductase, as a potential key player in thermophilic intracellular disulfide-bond formation. Finally, biochemical support in the form of a new crystal structure of a thermophilic protein with three disulfide bonds is presented together with a survey of known structures from the literature. Together, the results provide insight into biochemical specialization and the diversity of methods employed by organisms to stabilize their proteins in exotic environments. The findings also motivate continued efforts to sequence genomes from divergent organisms