54 research outputs found
Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment
BACKGROUND: Extra-cellular shear force is an important environmental parameter that is significant both medically and in the space environment. Escherichia coli cells grown in a low-shear modeled microgravity (LSMMG) environment produced in a high aspect rotating vessel (HARV) were subjected to transcriptional and physiological analysis. RESULTS: Aerobic LSMMG cultures were grown in rich (LB) and minimal (MOPS + glucose) medium with a normal gravity vector HARV control. Reproducible changes in transcription were seen, but no specific LSMMG responsive genes were identified. Instead, absence of shear and a randomized gravity vector appears to cause local extra-cellular environmental changes, which elicit reproducible cellular responses. In minimal media, the majority of the significantly up- or down-regulated genes of known function were associated with the cell envelope. In rich medium, most LSMMG down-regulated genes were involved in translation. No observable changes in post-culture stress responses and antibiotic sensitivity were seen in cells immediately after exposure to LSMMG. Comparison with earlier studies of Salmonella enterica serovar Typhimurium conducted under similar growth conditions, revealed essentially no similarity in the genes that were significantly up- or down-regulated. CONCLUSION: Comparison of these results to previous studies suggests that different organisms may dramatically differ in their responses to medically significant low-shear and space environments. Depending on their specific response, some organisms, such as Salmonella, may become preadapted in a manner that predisposes them to increased virulence
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Lake heatwaves under climate change
Lake ecosystems, and the organisms that live within them, are vulnerable to temperature change1,2,3,4,5, including the increased occurrence of thermal extremes6. However, very little is known about lake heatwaves—periods of extreme warm lake surface water temperature—and how they may change under global warming. Here we use satellite observations and a numerical model to investigate changes in lake heatwaves for hundreds of lakes worldwide from 1901 to 2099. We show that lake heatwaves will become hotter and longer by the end of the twenty-first century. For the high-greenhouse-gas-emission scenario (Representative Concentration Pathway (RCP) 8.5), the average intensity of lake heatwaves, defined relative to the historical period (1970 to 1999), will increase from 3.7 ± 0.1 to 5.4 ± 0.8 degrees Celsius and their average duration will increase dramatically from 7.7 ± 0.4 to 95.5 ± 35.3 days. In the low-greenhouse-gas-emission RCP 2.6 scenario, heatwave intensity and duration will increase to 4.0 ± 0.2 degrees Celsius and 27.0 ± 7.6 days, respectively. Surface heatwaves are longer-lasting but less intense in deeper lakes (up to 60 metres deep) than in shallower lakes during both historic and future periods. As lakes warm during the twenty-first century7,8, their heatwaves will begin to extend across multiple seasons, with some lakes reaching a permanent heatwave state. Lake heatwaves are likely to exacerbate the adverse effects of long-term warming in lakes and exert widespread influence on their physical structure and chemical properties. Lake heatwaves could alter species composition by pushing aquatic species and ecosystems to the limits of their resilience. This in turn could threaten lake biodiversity9 and the key ecological and economic benefits that lakes provide to society
Breed Locally, Disperse Globally: Fine-Scale Genetic Structure Despite Landscape-Scale Panmixia in a Fire- Specialist
Abstract An exciting advance in the understanding of metapopulation dynamics has been the investigation of how populations respond to ephemeral patches that go 'extinct' during the lifetime of an individual. Previous research has shown that this scenario leads to genetic homogenization across large spatial scales. However, little is known about fine-scale genetic structuring or how this changes over time in ephemeral patches. We predicted that species that specialize on ephemeral habitats will delay dispersal to exploit natal habitat patches while resources are plentiful and thus display fine-scale structure. To investigate this idea, we evaluated the effect of frequent colonization of ephemeral habitats on the fine-scale genetic structure of a fire specialist, the black-backed woodpecker (Picoides arcticus) and found a pattern of fine-scale genetic structure. We then tested for differences in spatial structure between sexes and detected a pattern consistent with male-biased dispersal. We also detected a temporal increase in relatedness among individuals within newly burned forest patches. Our results indicate that specialist species that outlive their ephemeral patches can accrue significant fine-scale spatial structure that does not necessarily affect spatial structure at larger scales. This highlights the importance of both spatial and temporal scale considerations in both sampling and data interpretation of molecular genetic results. Citation: Pierson JC, Allendorf FW, Drapeau P, Schwartz MK (2013) Breed Locally, Disperse Globally: Fine-Scale Genetic Structure Despite Landscape-Scale Panmixia in a Fire-Specialist. PLoS ONE 8(6): e67248
Drivers of phytoplankton responses to summer wind events in a stratified lake: A modeling study
Extreme wind events affect lake phytoplankton by deepening the mixed layer and increasing internal nutrient loading. Both increases and decreases in phytoplankton concentration after strong wind events have been observed, but the precise mechanisms driving these responses remain poorly understood or quantified. We coupled a one-dimensional physical model to a biogeochemical model to investigate the factors regulating short-term phytoplankton responses to summer wind events, now and under expected warmer future conditions. We simulated physical, chemical, and biological dynamics in Lake Erken, Sweden, and found that strong wind could increase or decrease the phytoplankton concentration in the euphotic zone 1 week after the event, depending on antecedent lake physical and chemical conditions. Wind had little effect on phytoplankton concentration if the mixed layer was deep prior to wind exposure. Higher incoming shortwave radiation and hypolimnetic nutrient concentration boosted phytoplankton concentration, whereas higher surface water temperatures decreased concentrations after wind events. Medium-intensity wind events resulted in more phytoplankton than high-intensity wind. Simulations under a future climate scenario did not show marked differences in the way wind events affect phytoplankton concentration. These findings help to better understand how wind impacts vary as a function of local environmental conditions and how climate warming and changing extreme weather dynamics will affect lake ecosystems
Drivers of phytoplankton responses to summer wind events in a stratified lake: A modeling study
Extreme wind events affect lake phytoplankton by deepening the mixed layer and increasing internal nutrient loading. Both increases and decreases in phytoplankton concentration after strong wind events have been observed, but the precise mechanisms driving these responses remain poorly understood or quantified. We coupled a one-dimensional physical model to a biogeochemical model to investigate the factors regulating short-term phytoplankton responses to summer wind events, now and under expected warmer future conditions. We simulated physical, chemical, and biological dynamics in Lake Erken, Sweden, and found that strong wind could increase or decrease the phytoplankton concentration in the euphotic zone 1 week after the event, depending on antecedent lake physical and chemical conditions. Wind had little effect on phytoplankton concentration if the mixed layer was deep prior to wind exposure. Higher incoming shortwave radiation and hypolimnetic nutrient concentration boosted phytoplankton concentration, whereas higher surface water temperatures decreased concentrations after wind events. Medium-intensity wind events resulted in more phytoplankton than high-intensity wind. Simulations under a future climate scenario did not show marked differences in the way wind events affect phytoplankton concentration. These findings help to better understand how wind impacts vary as a function of local environmental conditions and how climate warming and changing extreme weather dynamics will affect lake ecosystems
High adult mortality in disease-challenged frog populations increases vulnerability to drought
Pathogen emergence can drive major changes in host population demography, with implications for population dynamics and sensitivity to environmental fluctuations. The amphibian disease chytridiomycosis, caused by infection with the fungal pathogen Batrachochytrium dendrobatidis (Bd), is implicated in the severe decline of over 200 amphibian species. In species that have declined but not become extinct, Bd persists and can cause substantial ongoing mortality. High rates of mortality associated with Bd may drive major changes in host demography, but this process is poorly understood. Here, we compared population age structure of Bd-infected populations, Bd-free populations, and museum specimens collected prior to Bd emergence for the endangered Australian frog, Litoria verreauxii alpina (alpine tree frog). We then used population simulations to investigate how pathogen-associated demographic shifts affect the ability of populations to persist in stochastic environments. We found that Bd-infected populations have a severely truncated age structure associated with very high rates of annual adult mortality. Near-complete annual adult turnover in Bd-infected populations means that individuals breed once, compared with Bd-free populations where adults may breed across multiple years. Our simulations showed that truncated age structure erodes the capacity of populations to withstand periodic recruitment failure; a common challenge for species reproducing in uncertain environments. We document previously undescribed demographic shifts associated with a globally emerging pathogen and demonstrate how these shifts alter host ecology. Truncation of age structure associated with Bd effectively reduces host niche width, and can help explain the contraction of L. v. alpina to perennial waterbodies where the risk of drought-induced recruitment failure is low. Reduced capacity to tolerate other sources of mortality may explain variation in decline severity among other chytridiomycosis-challenged species and highlights the potential to mitigate disease impacts through minimising other sources of mortality
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Citizen science shows systematic changes in the temperature difference between air and inland waters with global warming
Citizen science projects have a long history in ecological studies. The research usefulness of such projects is dependent on applying simple and standardized methods. Here, we conducted a citizen science project that involved more than 3500 Swedish high school students to examine the temperature difference between surface water and the overlying air (Tw-Ta) as a proxy for sensible heat flux (QH). If QH is directed upward, corresponding to positive Tw-Ta, it can enhance CO2 and CH4 emissions from inland waters, thereby contributing to increased greenhouse gas concentrations in the atmosphere. The students found mostly negative Tw-Ta across small ponds, lakes, streams/rivers and the sea shore (i.e. downward QH), with Tw-Ta becoming increasingly negative with increasing Ta. Further examination of Tw-Ta using high-frequency temperature data from inland waters across the globe confirmed that Tw-Ta is linearly related to Ta. Using the longest available high-frequency temperature time series from Lake Erken, Sweden, we found a rapid increase in the occasions of negative Tw-Ta with increasing annual mean Ta since 1989. From these results, we can expect that ongoing and projected global warming will result in increasingly negative Tw-Ta, thereby reducing CO2 and CH4 transfer velocities from inland waters into the atmosphere
The Allen Telescope Array: The First Widefield, Panchromatic, Snapshot Radio Camera for Radio Astronomy and SETI
The first 42 elements of the Allen Telescope Array (ATA-42) are beginning to
deliver data at the Hat Creek Radio Observatory in Northern California.
Scientists and engineers are actively exploiting all of the flexibility
designed into this innovative instrument for simultaneously conducting surveys
of the astrophysical sky and conducting searches for distant technological
civilizations. This paper summarizes the design elements of the ATA, the cost
savings made possible by the use of COTS components, and the cost/performance
trades that eventually enabled this first snapshot radio camera. The
fundamental scientific program of this new telescope is varied and exciting;
some of the first astronomical results will be discussed.Comment: Special Issue of Proceedings of the IEEE: "Advances in Radio
Telescopes", Baars,J. Thompson,R., D'Addario, L., eds, 2009, in pres
The Allen Telescope Array Pi GHz Sky Survey I. Survey Description and Static Catalog Results for the Bootes Field
The Pi GHz Sky Survey (PiGSS) is a key project of the Allen Telescope Array.
PiGSS is a 3.1 GHz survey of radio continuum emission in the extragalactic sky
with an emphasis on synoptic observations that measure the static and
time-variable properties of the sky. During the 2.5-year campaign, PiGSS will
twice observe ~250,000 radio sources in the 10,000 deg^2 region of the sky with
b > 30 deg to an rms sensitivity of ~1 mJy. Additionally, sub-regions of the
sky will be observed multiple times to characterize variability on time scales
of days to years. We present here observations of a 10 deg^2 region in the
Bootes constellation overlapping the NOAO Deep Wide Field Survey field. The
PiGSS image was constructed from 75 daily observations distributed over a
4-month period and has an rms flux density between 200 and 250 microJy. This
represents a deeper image by a factor of 4 to 8 than we will achieve over the
entire 10,000 deg^2. We provide flux densities, source sizes, and spectral
indices for the 425 sources detected in the image. We identify ~100$ new flat
spectrum radio sources; we project that when completed PiGSS will identify 10^4
flat spectrum sources. We identify one source that is a possible transient
radio source. This survey provides new limits on faint radio transients and
variables with characteristic durations of months.Comment: Accepted for publication in ApJ; revision submitted with extraneous
figure remove
The Allen Telescope Array Twenty-centimeter Survey - A 690-Square-Degree, 12-Epoch Radio Dataset - I: Catalog and Long-Duration Transient Statistics
We present the Allen Telescope Array Twenty-centimeter Survey (ATATS), a
multi-epoch (12 visits), 690 square degree radio image and catalog at 1.4GHz.
The survey is designed to detect rare, very bright transients as well as to
verify the capabilities of the ATA to form large mosaics. The combined image
using data from all 12 ATATS epochs has RMS noise sigma = 3.94mJy / beam and
dynamic range 180, with a circular beam of 150 arcsec FWHM. It contains 4408
sources to a limiting sensitivity of S = 20 mJy / beam. We compare the catalog
generated from this 12-epoch combined image to the NRAO VLA Sky Survey (NVSS),
a legacy survey at the same frequency, and find that we can measure source
positions to better than ~20 arcsec. For sources above the ATATS completeness
limit, the median flux density is 97% of the median value for matched NVSS
sources, indicative of an accurate overall flux calibration. We examine the
effects of source confusion due to the effects of differing resolution between
ATATS and NVSS on our ability to compare flux densities. We detect no
transients at flux densities greater than 40 mJy in comparison with NVSS, and
place a 2-sigma upper limit on the transient rate for such sources of 0.004 per
square degree. These results suggest that the > 1 Jy transients reported by
Matsumura et al. (2009) may not be true transients, but rather variable sources
at their flux density threshold.Comment: 41 pages, 19 figures, ApJ accepted; corrected minor typo in Table
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