Amplified singlet oxygen generation in metallated-porphyrin doped conjugated polymer nanoparticles

We report on the mechanism and efficiencies of singlet oxygen O2(1Δg) generation of nanoparticles (NP) of the conjugated polymer (CP) poly(9,9-dioctylfluorene-alt-benzothiadiazole) (F8BT) doped with platinum octaethylporphyrin (PtOEP) suspended in water. A detailed study of the photophysics of these NP, using stationary and time-resolved absorption and emission techniques, indicates that O2(1Δg) is generated by the triplet excited state of F8BT and not by that of PtOEP, as previously observed for other porphyrin doped CP NP. O2(1Δg) quantum yields (ΦΔ) were measured by quantifying the characteristic phosphorescence of O2(1Δg) in the NIR region (∼1268 nm). It was found that incorporation of relatively small amounts of PtOEP to F8BT NP results in a significant increase of ΦΔ. NP containing 10% PtOEP (w/w) show a ΦΔ ∼ 0.24, which is 3 times larger than that observed for undoped F8BT NP, and larger than the reported for most water-soluble porphyrins. ΦΔ were also calculated from the oxidation rates (v0) of 3-[10-(2-carboxyethyl)anthracen-9-yl]propanoic acid (ADPA), a well-known chemical O2(1Δg) trap. Unexpectedly, this method was found to significantly overestimate the ΦΔ values due to the adsorption of ADPA on the surface of NP. The ADPA/NP adsorption process was characterized using a simple adsorption model yielding an (average) equilibrium constant of ∼8 × 103 M−1 and an (average) number of NP-binding sites of ∼14000. These results necessarily caution about the use of ADPA as a probe to evaluate ΦΔ in these NP systems. In addition, the interaction of F8BT NP with other anionic, cationic and zwitterionic dyes (dissolved in water) was studied. It was found that even at nano-molar concentrations all the dyes efficiently adsorb on the NP surface. This general and simple self-assembly strategy can be used to prepare superficially-dye-doped CP NP with potentially interesting technological applications.Fil: Spada, Ramiro Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Macor, Lorena Paola. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hernández, Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ponzio, Rodrigo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Ibarra, Luis Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Lorente, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Chesta, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Palacios, Rodrigo Emiliano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Catechol-Containing acrylic poly(ionic liquid) hydrogels as bioinspired Filters for Water decontamination

Mussel inspired catechol containing materials have currently drawn great attention as biomaterials, adhesives, surface coatings and in bioelectronics, among other applications. In this work, we mimicked the ability of mussels as water filtration systems to adsorb organic and inorganic contaminants. For this purpose, the synthesis of biomimetic hydrogels by co-polymerization of a new ionic monomer, dopamine methacrylic acid salt (iDA) with a series of water soluble methacrylate monomers was performed using visible light photopolymerization. The iDA ionic monomer is highly water soluble as compared to previous reported monomers containing catechol groups. This allows its incorporation into different acrylic hydrogels in concentrations up to 50 % mol of monomers containing catechol groups, leading to functional materials with variable morphology and swelling properties. The hydrogels displayed to be highly effective for the removal of heavy metals such as As(V) and Cr(VI) with very good effectiveness compared to other commonly employed natural sorbents, such as clays. Additionally, these poly(ionic liquid) hydrogels containing catechol groups were evaluated in the removal also of other pollutants such as charged organic dyes. Preliminarily results demonstrate the versatility of these materials that combine catechol and ionic chemistry for the adsorption of a wide variety of water pollutants.Fil: Gallastegui, Antonela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Porcarelli, Luca. Polymat University Of The Basque ; EspañaFil: Palacios, Rodrigo Emiliano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Soulé Gómez, María Lorena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Mecerreyes Molero, David. Polymat University Of The Basque ; Españ

Iron oxide incorporated conjugated polymer nanoparticles for simultaneous use in magnetic resonance and fluorescent imaging of brain tumors

Conjugated polymer nanoparticles (CPNs) have emerged as advanced polymeric nanoplat-forms in biomedical applications by virtue of extraordinary properties including high fluorescence brightness, large absorption coefficients of one and two-photons, and excellent photostability and colloidal stability in water and physiological medium. In addition, low cytotoxicity, easy functional-ization, and the ability to modify CPN photochemical properties by the incorporation of dopants, convert them into excellent theranostic agents with multifunctionality for imaging and treatment. In this work, CPNs were designed and synthesized by incorporating a metal oxide magnetic core (Fe3 O4 and NiFe2 O4 nanoparticles, 5 nm) into their matrix during the nanoprecipitation method. This modification allowed the in vivo monitoring of nanoparticles in animal models using magnetic resonance imaging (MRI) and intravital fluorescence, techniques widely used for intracranial tumors evaluation. The modified CPNs were assessed in vivo in glioblastoma (GBM) bearing mice, both heterotopic and orthotopic developed models. Biodistribution studies were performed with MRI acquisitions and fluorescence images up to 24 h after the i.v. nanoparticles administration. The resulting IONP-doped CPNs were biocompatible in GBM tumor cells in vitro with an excellent cell incorporation depending on nanoparticle concentration exposure. IONP-doped CPNs were detected in tumor and excretory organs of the heterotopic GBM model after i.v. and i.t. injection. However, in the orthotopic GBM model, the size of the nanoparticles is probably hindering a higher effect on intratumorally T2-weighted images (T2 WI) signals and T2 values. The photodynamic therapy (PDT)—cytotoxicity of CPNs was not either affected by the IONPs incorporation into the nanoparticles.Fil: Arias Ramos, Nuria. Consejo Superior de Investigaciones Científicas; EspañaFil: Ibarra, Luis Exequiel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Serrano Torres, María. Consejo Superior de Investigaciones Científicas; EspañaFil: Yagüe, Balbino. Consejo Superior de Investigaciones Científicas; EspañaFil: Caverzan, Matias Daniel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Chesta, Carlos Alberto. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Palacios, Rodrigo Emiliano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: López Larrubia, Pilar. Consejo Superior de Investigaciones Científicas; Españ

Amplified singlet oxygen generation in metallated-porphyrin doped conjugated polymer nanoparticles

Abstract We report on the mechanism and efficiencies of singlet oxygen O2(1Δg) generation of nanoparticles (NP) of the conjugated polymer (CP) poly(9,9-dioctylfluorene- alt -benzothiadiazole) (F8BT) doped with platinum octaethylporphyrin (PtOEP) suspended in water. A detailed study of the photophysics of these NP, using stationary and time-resolved absorption and emission techniques, indicates that O2(1Δg) is generated by the triplet excited state of F8BT and not by that of PtOEP, as previously observed for other porphyrin doped CP NP. O2 (1Δg) quantum yields (ΦΔ) were measured by quantifying the characteristic phosphorescence of O2(1Δg) in the NIR region (∼1268 nm). It was found that incorporation of relatively small amounts of PtOEP to F8BT NP results in a significant increase of ΦΔ. NP containing 10% PtOEP (w/w) show a ΦΔ ∼ 0.24, which is 3 times larger than that observed for undoped F8BT NP, and larger than the reported for most water-soluble porphyrins. ΦΔ were also calculated from the oxidation rates ( v 0 ) of 3-[10-(2-carboxyethyl)anthracen-9-yl]propanoic acid (ADPA), a well-known chemical O2(1Δg) trap. Unexpectedly, this method was found to significantly overestimate the ΦΔ values due to the adsorption of ADPA on the surface of NP. The ADPA/NP adsorption process was characterized using a simple adsorption model yielding an (average) equilibrium constant of ∼8 × 103 M−1 and an (average) number of NP-binding sites of ∼14000. These results necessarily caution about the use of ADPA as a probe to evaluate ΦΔ in these NP systems. In addition, the interaction of F8BT NP with other anionic, cationic and zwitterionic dyes (dissolved in water) was studied. It was found that even at nano-molar concentrations all the dyes efficiently adsorb on the NP surface. This general and simple self-assembly strategy can be used to prepare superficially-dye-doped CP NP with potentially interesting technological applications.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicada

VIII Encuentro de Docentes e Investigadores en Historia del Diseño, la Arquitectura y la Ciudad

Acta de congresoLa conmemoración de los cien años de la Reforma Universitaria de 1918 se presentó como una ocasión propicia para debatir el rol de la historia, la teoría y la crítica en la formación y en la práctica profesional de diseñadores, arquitectos y urbanistas. En ese marco el VIII Encuentro de Docentes e Investigadores en Historia del Diseño, la Arquitectura y la Ciudad constituyó un espacio de intercambio y reflexión cuya realización ha sido posible gracias a la colaboración entre Facultades de Arquitectura, Urbanismo y Diseño de la Universidad Nacional y la Facultad de Arquitectura de la Universidad Católica de Córdoba, contando además con la activa participación de mayoría de las Facultades, Centros e Institutos de Historia de la Arquitectura del país y la región. Orientado en su convocatoria tanto a docentes como a estudiantes de Arquitectura y Diseño Industrial de todos los niveles de la FAUD-UNC promovió el debate de ideas a partir de experiencias concretas en instancias tales como mesas temáticas de carácter interdisciplinario, que adoptaron la modalidad de presentación de ponencias, entre otras actividades. En el ámbito de VIII Encuentro, desarrollado en la sede Ciudad Universitaria de Córdoba, se desplegaron numerosas posiciones sobre la enseñanza, la investigación y la formación en historia, teoría y crítica del diseño, la arquitectura y la ciudad; sumándose el aporte realizado a través de sus respectivas conferencias de Ana Clarisa Agüero, Bibiana Cicutti, Fernando Aliata y Alberto Petrina. El conjunto de ponencias que se publican en este Repositorio de la UNC son el resultado de dos intensas jornadas de exposiciones, cuyos contenidos han posibilitado actualizar viejos dilemas y promover nuevos debates. El evento recibió el apoyo de las autoridades de la FAUD-UNC, en especial de la Secretaría de Investigación y de la Biblioteca de nuestra casa, como así también de la Facultad de Arquitectura de la UCC; va para todos ellos un especial agradecimiento

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Heterogeneous Charge Mobility in Individual Conjugated Polyelectrolyte Nanoparticles Revealed by Two-Color Single Particle Spectroelectrochemistry Studies

The optoelectronic properties of conjugated polymers and conjugated polyelectrolytes (CPEs) depend on their chain conformation and packing. Correlations between emission color, charge mobility, and extent of aggregation in these materials have been previously established from bulk studies. Here we describe the preparation of stable nanoparticle suspensions of the CPE poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylenevinylene (MPS-PPV) where changes in the solvent composition enable tuning their emission spectra and quantum yield. By employing a newly developed color-sensitive single-molecule spectroelectrochemistry (SMS-EC) technique, the effect of chain conformation on the optoelectronic properties of MPS-PPV nanoparticles is monitored at the single particle level. Within a single particle the photoluminescence and redox response is chromatically correlated reflecting on the differing contributions that coiled and deaggregated vs extended and packed segments have on their optoelectronic properties. We also observe a heterogeneous response among nanoparticles to externally applied electrochemical potentials, which further correlates with their emission color (chain packing). We rationalize our observations on differential charge injection, energy and charge transport, and ion migration as a consequence of chain conformation, packing effects, and the presence of electrochemically reducible quenching sites. Our work provides a way to unravel the intrinsic heterogeneity of CPE materials to better understand the relationship between chain conformation and optoelectronic properties.Fil: Godin, Robert. McGill University; CanadáFil: Palacios, Rodrigo Emiliano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Cosa, Gonzalo. McGill University; Canad

The Therapeutic Revolution of Conjugated Polymer Nanoparticles in Photodynamic Therapy and Photodynamic Inactivation

Compared to traditional anticancer and antimicrobial therapies, photodynamic therapy (PDT) and photodynamic inactivation (PDI) arise as improved treatment tools due to their highly effective, non-invasive and localized therapeutic action. These therapies simultaneously combine three elements: i) photosensitizer (PS), ii) light and iii) molecular oxygen to produce reactive oxygen species (ROS). These oxygen species can produce biomolecular damage that leads to eukaryotic and prokaryotic cell death. Moreover, nanotechnology has been used for light-mediated anticancer and antibacterial strategies to overcome inherent limitations of small molecule PSs, such as poor solubility in biological media, nontargeted delivery, and inefficient photoinduced generation of ROS. In this sense, conjugated polymer nanoparticles (CPNs) have emerged as advanced PSs used in PDT and PDI treatments. Conjugated polymers (CP) are organic macromolecules formed by a series of repetitive monomers concatenated together by a succession of single and double (or triple) bonds alternated along the chain. The polymer main chain has segments of variable length where the delocalization of the π electrons is preserved acting as “quasi-chromophores.” CPNs are formed by folding/collapsing of CP hydrophobic chains in a poor solvent (water) to form nanoaggregates. These nanoaggregates act as densely packed multichromophoric systems with exceptional light harvesting and (intraparticle) energy transfer capabilities which can lead to efficient photosensitized formation of ROS when effectively exploited. Additionally, CPNs have a number of properties which are highly desirable for PDT, PDI and theranostics applications, such as small size (10-50 nm) with narrow distribution, nearly null cytotoxicity, high fluorescence brightness, large absorption coefficients of one and twophotons, and easily tuned optical and photochemical properties by the incorporation of molecular dopants. A brief review of the literature shows that CPNs have been increasingly used as advanced PSs for cell labeling, anticancer treatment (PDT), and bacterial inactivation (PDI). This chapter aims to summarize recent advances, mainly from our laboratory, on the development of CPNs as advanced PSs for PDT and PDI applications.Fil: Ibarra, Luis Exequiel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Martinez, Sol Romina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Ponzio, Rodrigo Andrés. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Palacios, Rodrigo Emiliano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentin

Metronomic Photodynamic Therapy with Conjugated Polymer Nanoparticles in Glioblastoma Tumor Microenvironment

Alternative therapies such as photodynamic therapy (PDT) that combine light, oxygen and photosensitizers (PSs) have been proposed for glioblastoma (GBM) management to overcome conventional treatment issues. An important disadvantage of PDT using a high light irradiance (fluence rate) (cPDT) is the abrupt oxygen consumption that leads to resistance to the treatment. PDT metronomic regimens (mPDT) involving administering light at a low irradiation intensity over a relatively long period of time could be an alternative to circumvent the limitations of conventional PDT protocols. The main objective of the present work was to compare the effectiveness of PDT with an advanced PS based on conjugated polymer nanoparticles (CPN) developed by our group in two irradiation modalities: cPDT and mPDT. The in vitro evaluation was carried out based on cell viability, the impact on the macrophage population of the tumor microenvironment in co-culture conditions and the modulation of HIF-1α as an indirect indicator of oxygen consumption. mPDT regimens with CPNs resulted in more effective cell death, a lower activation of molecular pathways of therapeutic resistance and macrophage polarization towards an antitumoral phenotype. Additionally, mPDT was tested in a GBM heterotopic mouse model, confirming its good performance with promising tumor growth inhibition and apoptotic cell death induction.Fil: Caverzan, Matias Daniel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Oliveda, Paula Martina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Beaugé, Lucía. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Palacios, Rodrigo Emiliano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Chesta, Carlos Alberto. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Ibarra, Luis Exequiel. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentin