746 research outputs found
Substance P regulates puberty onset and fertility in the female mouse
Puberty is a tightly regulated process that leads to reproductive capacity. Kiss1 neurons are crucial in this process by stimulating GnRH, yet how Kiss1 neurons are regulated remains unknown. Substance P (SP), an important neuropeptide in pain perception, induces gonadotropin release in adult mice in a kisspeptin-dependent manner. Here, we assessed whether SP, through binding to its receptor NK1R (neurokinin 1 receptor), participates in the timing of puberty onset and fertility in the mouse. We observed that 1) selective NK1R agonists induce gonadotropin release in prepubertal females; 2) the expression of Tac1 (encoding SP) and Tacr1 (NK1R) in the arcuate nucleus is maximal before puberty, suggesting increased SP tone; 3) repeated exposure to NK1R agonists prepubertally advances puberty onset; and 4) female Tac1-/- mice display delayed puberty; moreover, 5) SP deficiency leads to subfertility in females, showing fewer corpora lutea and antral follicles and leading to decreased litter size. Thus, our findings support a role for SP in the stimulation of gonadotropins before puberty, acting via Kiss1 neurons to stimulate GnRH release, and its involvement in the attainment of full reproductive capabilities in female mice. Copyright © 2015 by the Endocrine Society
Faster Regular Expression Matching
Abstract. Regular expression matching is a key task (and often the computational bottleneck) in a variety of widely used software tools and applications, for instance, the unix grep and sed commands, scripting languages such as awk and perl, programs for analyzing massive data streams, etc. We show how to solve this ubiquitous task in linear space and O(nm(log log n)/(logn)3/2+n+m) time where m is the length of the expression and n the length of the string. This is the first improvement for the dominant O(nm / logn) term in Myers ’ O(nm / logn+ (n+m) logn) bound [JACM 1992]. We also get improved bounds for external memory.
Changes in hypothalamic expression of the Lin28/let-7 system and related MicroRNAs during postnatal maturation and after experimental manipulations of puberty
[Abstract] Lin28 and Lin28b are related RNA-binding proteins that inhibit the maturation of miRNAs of the let-7 family and participate in the control of cellular stemness and early embryonic development. Considerable interest has arisen recently concerning other physiological roles of the Lin28/let-7 axis, including its potential involvement in the control of puberty, as suggested by genome-wide association studies and functional genomics. We report herein the expression profiles of Lin28 and let-7 members in the rat hypothalamus during postnatal maturation and in selected models of altered puberty. The expression patterns of c-Myc (upstream positive regulator of Lin28), mir-145 (negative regulator of c-Myc), and mir-132 and mir-9 (putative miRNA repressors of Lin28, predicted by bioinformatic algorithms) were also explored. In male and female rats, Lin28, Lin28b, and c-Myc mRNAs displayed very high hypothalamic expression during the neonatal period, markedly decreased during the infantile-to-juvenile transition and reached minimal levels before/around puberty. A similar puberty-related decline was observed for Lin28b in monkey hypothalamus but not in the rat cortex, suggesting species conservation and tissue specificity. Conversely, let-7a, let-7b, mir-132, and mir-145, but not mir-9, showed opposite expression profiles. Perturbation of brain sex differentiation and puberty, by neonatal treatment with estrogen or androgen, altered the expression ratios of Lin28/let-7 at the time of puberty. Changes in the c-Myc/Lin28b/let-7 pathway were also detected in models of delayed puberty linked to early photoperiod manipulation and, to a lesser extent, postnatal underfeeding or chronic subnutrition. Altogether, our data are the first to document dramatic changes in the expression of the Lin28/let-7 axis in the rat hypothalamus during the postnatal maturation and after different manipulations that disturb puberty, thus suggesting the potential involvement of developmental changes in hypothalamic Lin28/let-7 expression in the mechanisms permitting/leading to puberty onset.Ministerio de Economia y Competitividad; BFU 2008-00984Ministerio de Economia y Competitividad; BFU 2011-25021Junta de Andalucía; P08-CVI-03788United States. National Institutes of Health HD025123-ARRAUnited States. National Science Foundation; IOS1121691Instituto de Salud Carlos III; PI10/00088Xunta de Galicia; IN845B-2010/187Xunta de Galicia; 10CSA916014P
Inflation, cold dark matter, and the central density problem
A problem with high central densities in dark halos has arisen in the context
of LCDM cosmologies with scale-invariant initial power spectra. Although n=1 is
often justified by appealing to the inflation scenario, inflationary models
with mild deviations from scale-invariance are not uncommon and models with
significant running of the spectral index are plausible. Even mild deviations
from scale-invariance can be important because halo collapse times and
densities depend on the relative amount of small-scale power. We choose several
popular models of inflation and work out the ramifications for galaxy central
densities. For each model, we calculate its COBE-normalized power spectrum and
deduce the implied halo densities using a semi-analytic method calibrated
against N-body simulations. We compare our predictions to a sample of dark
matter-dominated galaxies using a non-parametric measure of the density. While
standard n=1, LCDM halos are overdense by a factor of 6, several of our example
inflation+CDM models predict halo densities well within the range preferred by
observations. We also show how the presence of massive (0.5 eV) neutrinos may
help to alleviate the central density problem even with n=1. We conclude that
galaxy central densities may not be as problematic for the CDM paradigm as is
sometimes assumed: rather than telling us something about the nature of the
dark matter, galaxy rotation curves may be telling us something about inflation
and/or neutrinos. An important test of this idea will be an eventual consensus
on the value of sigma_8, the rms overdensity on the scale 8 h^-1 Mpc. Our
successful models have values of sigma_8 approximately 0.75, which is within
the range of recent determinations. Finally, models with n>1 (or sigma_8 > 1)
are highly disfavored.Comment: 13 pages, 6 figures. Minor changes made to reflect referee's
Comments, error in Eq. (18) corrected, references updated and corrected,
conclusions unchanged. Version accepted for publication in Phys. Rev. D,
scheduled for 15 August 200
Dark matter and Colliders searches in the MSSM
We study the complementarity between dark matter experiments (direct
detection and indirect detections) and accelerator facilities (the CERN LHC and
a TeV Linear Collider) in the framework of the
constrained Minimal Supersymmetric Standard Model (MSSM). We show how
non--universality in the scalar and gaugino sectors can affect the experimental
prospects to discover the supersymmetric particles. The future experiments will
cover a large part of the parameter space of the MSSM favored by WMAP
constraint on the relic density, but there still exist some regions beyond
reach for some extreme (fine tuned) values of the supersymmetric parameters.
Whereas the Focus Point region characterized by heavy scalars will be easily
probed by experiments searching for dark matter, the regions with heavy
gauginos and light sfermions will be accessible more easily by collider
experiments. More informations on both supersymmetry and astrophysics
parameters can be thus obtained by correlating the different signals.Comment: 25 pages, 10 figures, corrected typos and reference adde
Multiwavelength studies of MHD waves in the solar chromosphere: An overview of recent results
The chromosphere is a thin layer of the solar atmosphere that bridges the
relatively cool photosphere and the intensely heated transition region and
corona. Compressible and incompressible waves propagating through the
chromosphere can supply significant amounts of energy to the interface region
and corona. In recent years an abundance of high-resolution observations from
state-of-the-art facilities have provided new and exciting ways of
disentangling the characteristics of oscillatory phenomena propagating through
the dynamic chromosphere. Coupled with rapid advancements in
magnetohydrodynamic wave theory, we are now in an ideal position to thoroughly
investigate the role waves play in supplying energy to sustain chromospheric
and coronal heating. Here, we review the recent progress made in
characterising, categorising and interpreting oscillations manifesting in the
solar chromosphere, with an impetus placed on their intrinsic energetics.Comment: 48 pages, 25 figures, accepted into Space Science Review
Development of photocatalytic coatings for building materials with Bi2O3-ZnO nanoparticles
The aim of this study was to develop versatile coatings that can protect the stone surfaces of Ar-chitectural Heritage. Two different 3D media, namely superhydrophobic (SPHB) and hydrooleophobic (OHB), were utilized as host matrices for nanostructured photocatalysts (Bi2O3-ZnO 8/92). These photocatalysts were sensitive to visible light to enhance their efficiency when exposed to sunlight. To prevent the nanophotocatalyst from clumping together in the 3D media, non-ionic dispersant additives (Tween20, TritonX-100, and Brij35) were incorporated. The optimized suspensions were then applied to various substrates such as sandstone, limestone, and granite. The effectiveness of the coatings was assessed by evaluating the hydrophobicity, oleo-phobicity, and photocatalytic activity of the coated substrates. The Bi2O3-ZnO photocatalyst exhib-ited higher activity in the SPHB medium compared to the OHB medium. To simulate real-life con-ditions, the coated substrates were subjected to accelerated weathering tests to predict their dura-bility. Despite a significant reduction in their thickness, the coatings demonstrated sustained hy-drophobic efficiency and self-cleaning capability after the accelerated ageing tests
Update on the correlation of the highest energy cosmic rays with nearby extragalactic matter
Data collected by the Pierre Auger Observatory through 31 August 2007 showed
evidence for anisotropy in the arrival directions of cosmic rays above the
Greisen-Zatsepin-Kuz'min energy threshold, \nobreak{eV}. The
anisotropy was measured by the fraction of arrival directions that are less
than from the position of an active galactic nucleus within 75 Mpc
(using the V\'eron-Cetty and V\'eron catalog). An updated
measurement of this fraction is reported here using the arrival directions of
cosmic rays recorded above the same energy threshold through 31 December 2009.
The number of arrival directions has increased from 27 to 69, allowing a more
precise measurement. The correlating fraction is , compared
with expected for isotropic cosmic rays. This is down from the early
estimate of . The enlarged set of arrival directions is
examined also in relation to other populations of nearby extragalactic objects:
galaxies in the 2 Microns All Sky Survey and active galactic nuclei detected in
hard X-rays by the Swift Burst Alert Telescope. A celestial region around the
position of the radiogalaxy Cen A has the largest excess of arrival directions
relative to isotropic expectations. The 2-point autocorrelation function is
shown for the enlarged set of arrival directions and compared to the isotropic
expectation.Comment: Accepted for publication in Astroparticle Physics on 31 August 201
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