Strength and Durability of Hybrid Fibre Reinforced Binary Blend Geopolymer Concrete

This paper deals with an investigation on the influence of hybrid fibres on the strength and durability of binary blend geopolymer concrete. Geopolymer concrete was prepared using Fly ash and GGBS as source material and mix design was carried out as per the guidelines of Professor Rangan of Curtin University, Australia. Different fly ash-GGBS proportions namely 50-50%, 60-40%, 70-30%, 80-20% and 90-10% were considered. The tests on Geopolymer concrete reveal that the compressive strength increases as the percentage of GGBS increases up to 30%. Hence the combination of 70% fly ash and 30% GGBS was considered as a base material. In order to improve the engineering properties of the Geopolymer concrete, hybrid fibres which consist of metallic and non-metallic fibres were introduced in the base material. Metallic fibres considered was crimped steel fibres with different percentages of volume fraction viz. 0.5% and 1.0% and non-metallic fibres considered was basalt fibres with different percentages of volume fraction viz. 0.1%, 0.2% and 0.3%. Strength parameters such as compressive strength, split tensile strength, modulus of rupture and modulus of elasticity were obtained for various combinations of steel and basalt fibres. Similarly durability parameters which include permeability, water absorption, marine attack and sulphuric acid attack were also evaluated. The combination of fibres having 0.5% steel and 0.3% basalt gave better results with regard to both strength and durability of binary blend Geopolymer concrete

Cancer cells adapt FAM134B/BiP mediated ER-phagy to survive hypoxic stress

In the tumor microenvironment, cancer cells experience hypoxia resulting in the accumulation of misfolded/unfolded proteins largely in the endoplasmic reticulum (ER). Consequently, ER proteotoxicity elicits unfolded protein response (UPR) as an adaptive mechanism to resolve ER stress. In addition to canonical UPR, proteotoxicity also stimulates the selective, autophagy-dependent, removal of discrete ER domains loaded with misfolded proteins to further alleviate ER stress. These mechanisms can favor cancer cell growth, metastasis, and long-term survival. Our investigations reveal that during hypoxia-induced ER stress, the ER-phagy receptor FAM134B targets damaged portions of ER into autophagosomes to restore ER homeostasis in cancer cells. Loss of FAM134B in breast cancer cells results in increased ER stress and reduced cell proliferation. Mechanistically, upon sensing hypoxia-induced proteotoxic stress, the ER chaperone BiP forms a complex with FAM134B and promotes ER-phagy. To prove the translational implication of our mechanistic findings, we identified vitexin as a pharmacological agent that disrupts FAM134B-BiP complex, inhibits ER-phagy, and potently suppresses breast cancer progression in vivo

Behaviour of Hybrid Fibre-Reinforced Ternary Blend Geopolymer Concrete Beam-Column Joints under Reverse Cyclic Loading

Beam–column joints are extremely vulnerable to lateral and vertical loads in reinforced concrete (RC) structures. This insufficiency in joint performance can lead to the failure of the whole structure in the event of unforeseen seismic and wind loads. This experimental work was conducted to study the behaviour of ternary blend geopolymer concrete (TGPC) beam-column joints with the addition of hybrid fibres, viz., steel and polypropylene fibres, under reverse cyclic loads. Nine RC beam-column joints were prepared and tested under reverse cyclic loading to recreate the conditions during an earthquake. M55 grade TGPC was designed and used in this present study. The primary parameters studied in this experimental investigation were the volume fractions of steel fibres (0.5% and 1.0%) and polypropylene fibres, viz., 0.1 to 0.25%, with an increment of 0.05%. In this study, the properties of hybrid fibre-reinforced ternary blend geopolymer concrete (HTGPC) beam-column joints, such as their ductility, energy absorption capacity, initial crack load and peak load carrying capacity, were investigated. The test results imply that the hybridisation of fibres effectively enhances the joint performance of TGPC. Also, an effort was made to compare the shear strength of HTGPC beam-column connections with existing equations from the literature. As the available models did not match the actual test results, a method was performed to obtain the shear strength of HTGPC beam-column connections. The developed equation was found to compare convincingly with the experimental test results

Influence of Steel Fibers on the Interfacial Shear Strength of Ternary Blend Geopolymer Concrete Composite

Sustainable development is a major issue confronting society today. Cement, a major constituent of concrete, is a key component of any infrastructure development. The major drawback of cement production is that it involves the emission of CO2, the predominant greenhouse gas causing global warming. The development of geopolymers has resulted in a decrease in cement production, as well as a reduction in CO2 emissions. During mass concrete production in the construction of very large structures, interfaces/joints are formed, which are potential failure sites of crack formation. Concrete may interface with other concrete of different strengths, or other construction materials, such as steel. To ensure the monolithic behavior of composite concrete structures, bond strength at the interface should be established. The monolithic behavior can be ensured by the usage of shear ties across the interface. However, an increase in the number of shear ties at the interface may reduce the construction efficiency. The present study aims to determine the interfacial shear strength of geopolymer concrete as a substrate, and high-strength concrete as an overlay, by adding 0.50%, 0.75%, and 1% crimped steel fibers, and two and three shear ties, at the interface of push-off specimens. It was found that three shear ties at the interface can be replaced by two shear ties and 0.75% crimped steel fibers. In addition, a method was proposed to predict the interface shear strength of the concrete composite, which was found to be comparable to the test results

Influence of Steel Fibers on the Interfacial Shear Strength of Ternary Blend Geopolymer Concrete Composite

Sustainable development is a major issue confronting society today. Cement, a major constituent of concrete, is a key component of any infrastructure development. The major drawback of cement production is that it involves the emission of CO2, the predominant greenhouse gas causing global warming. The development of geopolymers has resulted in a decrease in cement production, as well as a reduction in CO2 emissions. During mass concrete production in the construction of very large structures, interfaces/joints are formed, which are potential failure sites of crack formation. Concrete may interface with other concrete of different strengths, or other construction materials, such as steel. To ensure the monolithic behavior of composite concrete structures, bond strength at the interface should be established. The monolithic behavior can be ensured by the usage of shear ties across the interface. However, an increase in the number of shear ties at the interface may reduce the construction efficiency. The present study aims to determine the interfacial shear strength of geopolymer concrete as a substrate, and high-strength concrete as an overlay, by adding 0.50%, 0.75%, and 1% crimped steel fibers, and two and three shear ties, at the interface of push-off specimens. It was found that three shear ties at the interface can be replaced by two shear ties and 0.75% crimped steel fibers. In addition, a method was proposed to predict the interface shear strength of the concrete composite, which was found to be comparable to the test results

Cancer cells adapt FAM134B/BiP mediated ER-phagy to survive hypoxic stress

In the tumor microenvironment, cancer cells experience hypoxia resulting in the accumulation of misfolded/unfolded proteins largely in the endoplasmic reticulum (ER). Consequently, ER proteotoxicity elicits unfolded protein response (UPR) as an adaptive mechanism to resolve ER stress. In addition to canonical UPR, proteotoxicity also stimulates the selective, autophagy-dependent, removal of discrete ER domains loaded with misfolded proteins to further alleviate ER stress. These mechanisms can favor cancer cell growth, metastasis, and long-term survival. Our investigations reveal that during hypoxia-induced ER stress, the ER-phagy receptor FAM134B targets damaged portions of ER into autophagosomes to restore ER homeostasis in cancer cells. Loss of FAM134B in breast cancer cells results in increased ER stress and reduced cell proliferation. Mechanistically, upon sensing hypoxia-induced proteotoxic stress, the ER chaperone BiP forms a complex with FAM134B and promotes ER-phagy. To prove the translational implication of our mechanistic findings, we identified vitexin as a pharmacological agent that disrupts FAM134B-BiP complex, inhibits ER-phagy, and potently suppresses breast cancer progression in vivo