Egypt’s 2011–2012 parliamentary elections: Voting for religious vs. secular democracy?

This study investigates whether individuals’ attitudes towards democracy and secular politics have any influence on voting behavior in Egypt. Based on data from a survey conducted immediately after the Egyptian parliamentary elections in January 2012, this study finds that Egyptians’ attitudes towards democratic governance were quite negative around the parliamentary elections, yet Egyptians still endorsed democracy as the ideal political system for their country. However, empirical findings suggest that support for democracy has a limited impact on electoral results. On the other hand, the main division in Egyptian society around the first free and fair parliamentary elections was the religious-secular cleavage. As people support secular politics more, they become significantly less likely to vote for Islamist parties. These results illustrate that preferences in regard to the type of democracy – either a liberal and secular or a religious democracy – were the main determinant of the historic 2012 elections in Egypt

Dysconnection in schizophrenia: from abnormal synaptic plasticity to failures of self-monitoring

Over the last 2 decades, a large number of neurophysiological and neuroimaging studies of patients with schizophrenia have furnished in vivo evidence for dysconnectivity, ie, abnormal functional integration of brain processes. While the evidence for dysconnectivity in schizophrenia is strong, its etiology, pathophysiological mechanisms, and significance for clinical symptoms are unclear. First, dysconnectivity could result from aberrant wiring of connections during development, from aberrant synaptic plasticity, or from both. Second, it is not clear how schizophrenic symptoms can be understood mechanistically as a consequence of dysconnectivity. Third, if dysconnectivity is the primary pathophysiology, and not just an epiphenomenon, then it should provide a mechanistic explanation for known empirical facts about schizophrenia. This article addresses these 3 issues in the framework of the dysconnection hypothesis. This theory postulates that the core pathology in schizophrenia resides in aberrant N-methyl-D-aspartate receptor (NMDAR)–mediated synaptic plasticity due to abnormal regulation of NMDARs by neuromodulatory transmitters like dopamine, serotonin, or acetylcholine. We argue that this neurobiological mechanism can explain failures of self-monitoring, leading to a mechanistic explanation for first-rank symptoms as pathognomonic features of schizophrenia, and may provide a basis for future diagnostic classifications with physiologically defined patient subgroups. Finally, we test the explanatory power of our theory against a list of empirical facts about schizophrenia

The Origin of Intraspecific Variation of Virulence in an Eukaryotic Immune Suppressive Parasite

Occurrence of intraspecific variation in parasite virulence, a prerequisite for coevolution of hosts and parasites, has largely been reported. However, surprisingly little is known of the molecular bases of this variation in eukaryotic parasites, with the exception of the antigenic variation used by immune-evading parasites of mammals. The present work aims to address this question in immune suppressive eukaryotic parasites. In Leptopilina boulardi, a parasitic wasp of Drosophila melanogaster, well-defined virulent and avirulent strains have been characterized. The success of virulent females is due to a major immune suppressive factor, LbGAP, a RacGAP protein present in the venom and injected into the host at oviposition. Here, we show that an homologous protein, named LbGAPy, is present in the venom of the avirulent strain. We then question whether the difference in virulence between strains originates from qualitative or quantitative differences in LbGAP and LbGAPy proteins. Results show that the recombinant LbGAPy protein has an in vitro GAP activity equivalent to that of recombinant LbGAP and similarly targets Drosophila Rac1 and Rac2 GTPases. In contrast, a much higher level of both mRNA and protein is found in venom-producing tissues of virulent parasitoids. The F1 offspring between virulent and avirulent strains show an intermediate level of LbGAP in their venom but a full success of parasitism. Interestingly, they express almost exclusively the virulent LbGAP allele in venom-producing tissues. Altogether, our results demonstrate that the major virulence factor in the wasp L. boulardi differs only quantitatively between virulent and avirulent strains, and suggest the existence of a threshold effect of this molecule on parasitoid virulence. We propose that regulation of gene expression might be a major mechanism at the origin of intraspecific variation of virulence in immune suppressive eukaryotic parasites. Understanding this variation would improve our knowledge of the mechanisms of transcriptional evolution currently under active investigation

Volunteered information on nature-based solutions — Dredging for data on deculverting

Much has been written about the potential contribution of citizen-science approaches to further urban environmental sustainability, and associated interventions such as nature-based solutions (NBS). Engagements between researchers and stakeholders relying on bottom-up information provision, for instance community mapping, are often purported to play a vital role in developing shared knowledge, achieving greater impact and stimulating innovation. However, relatively few studies within the realm of NBS have reported on experiences in using volunteered information, or their results. This reflects an important gap, not least because of the proliferation of proposals and bids that rely upon or integrate such methods into their approach. We report on experiences with gathering information using a ‘bottom-up’ map-based wiki tool, which effectively sought to crowd-source data, contributed by members of the public and professional stakeholders. As we approach the milestone of ‘10 years on’ from the inception of the website www.daylighting.org.uk, we reflect on our approach, the opportunities presented, constraints encountered, progress made and results delivered. This is contrasted with other resources and data-gathering projects having similar aims for different urban NBS. Findings are presented on the substantive issue of the uptake of deculverting as a particular form of NBS, including land-use contexts, scheme costs and achievement of stated objectives. Reflections are given on potential contributions of such methods in relation to other, more established approaches and new techniques in urban knowledge co-productio

Immunogenic and Antigenic Profiles of Nine Lactococcus garvieae Strains from Different Rainbow Trout Farms

The aims of this study were to determine differences with respect to immunogenic potency in the antigenic profiles of nine Lactococcus garvieae strains from Turkey, Spain, and England, to develop a bacterin, and to examine the immunological response of rainbow trout to the bacterin. The strains had identical Western blot patterns with 30, 37, 40, 46, 52, and 66 kDA mw protein bands. After culturing the bacteria, proteins were separated by SDS-PAGE and transferred to Immobilon membranes. The membranes were incubated with hyperimmune rabbit sera obtained by immunizing a rabbit against L. garvieae. One group of 50 fish was immunized with formalin- killed bacterin prepared from the most immunogenic strain of L. garvieae. A second group of 50 fish served as an unimmunized control. Four weeks after vaccination, both groups were chal- lenged intraperitoneally with a homologous strain. The protection rate of the bacterin was judged by the relative percent survival (RPS) of the groups. A significantly high level of protection was achieved in the vaccinated group (88.8% RPS)

Interleukin (IL) 5 levels and eosinophilia in patients with intestinal parasitic diseases

PubMed ID: 15534922Aim: Intestinal parasitic diseases are commonly accompanied with diarrhoeal symptoms and allergic reactions. Eosinophilia occurs as a result of IL-5 synthesized from Th2 cells during allergic reactions. IL-5 acts as a factor activating eosinophils. The aim of this study was to compare the IL-5 cytokine measurements in serum samples and cell cultures. And also to compare eosinophilia observed in helminth infections and protozoon infections accompanied with allergy. Methods: Twenty-three patients who presented with diarrhoeal symptoms and allergic complaints were tested positive for intestinal parasites, as well as 21 controls with allergic complaints who did not have any intestinal parasites were included in this study. TL-5 production in in vitro cell cultures prepared by using phytohemaglutinin (PHA) to stimulate peripheral blood mononuclear cells (PBMC) obtained from the blood samples taken from these patients were compared with the IL-5 level in serum. Furthermore, the IL-5 production in protozoon and helminth infections was also compared. Absolute eosinophil values in 1 mm3 of blood were calculated by means of peripheral smear in both groups within the scope of the study. Results: Parasites such as helminth detected in 15 (65.2%) and protozoon in 8 (34.8%) of the patients were included in this study. As regards the values of the sera in both patients with parasite infection and controls, the IL-5 production was found to be higher in the cell culture supernatant (P<0.001 and P<0.05). When the IL-5 level of the patients with helminth parasites was compared with that of those with protozoon, it was determined that the IL-5 level in serum was more significant in the patients with protozoon than in those with helminth (P<0.05). In the study group, the patients were found to have parasites, the percentage of eosinophil was 7.0% compared to 6.5% in the control group. Thus, there was no significant difference between the eosinophil values (P>0.05). Conclusion: It was found that IL-5 cytokine levels in serum samples from the patients with helminth and protozoon displayed more measurable values as compared to the IL-5 levels after stimulation with mitogen. It is concluded that IL-5 acts as a triggering factor in the toxiallergic complaints commonly seen in helminth and protozoon in infections. Copyright © 2004 by The WJG Press

The Effects of 201Tl Myocardial Perfusion Scintigraphy Studies on Oxidative Damage in Patients. (Efectos de los Estudios de Escintigrafía de Perfusión Miocárdica 201Tl Sobre el Daño Oxidativo en los Pacientes)

The aim of this study was to investigate gamma radiation-induced oxidative damage in erythrocytes after 201Tl myocardial perfusion scintigraphy. Twenty patients (8 women and 12 men) who performed 201Tl myocardial perfusion scintigraphy were included in this study. The blood samples were taken from patients just before, 1 hour after and three hours after injection of the radiopharmaceutical. Malondialdehyde (MDA) and antioxidant enzymes such as glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase (CAT) levels were measured to evaluate the gamma radiation induced oxidative damage. The enzyme activities of SOD, GPX and CAT were decreased 1 hour after (p = 0.042, p = 0. 697 and p = 0.653 respectively) and 3 hours after ( p = 0.003, p = 0. 573 and p = 0.002 respectively) injection of the radiopharmaceutical. Malondialdehyde levels were increased 1 hour after (p = 0.10) and 3 hours after (p = 0.47) injection of the radiopharmaceutical. In this study, we found that radiation due to 201Tl myocardial perfusion scintigraphy decreased the erythrocyte antioxidant levels and increased MDA levels. (Efectos de los Estudios de Escintigrafía de Perfusión Miocárdica 201Tl Sobre el Daño Oxidativo en los Pacientes) El objetivo de este estudio fue investigar el daño oxidativo inducido por radiaciones gamma, sobre los eritrocitos luego de realizada una escintigrafía de perfusión miocárdica 201Tl. Veinte pacientes (8 mujeres y 12 hombres) a quienes se les realizó escintigrafía por perfusión miocárdica 201Tl, fueron incluidos en este estudio. Las muestras de sangre fueron tomadas de los pacientes antes, 1 hora más tarde, y tres horas después de inyectar el radiofármaco. Se midieron los niveles del malondialdehido (MDA) y las enzimas antioxidantes tales como la glutationa peroxidasa (GPX), superóxido dismutasa (SOD), y la catalasa (CAT), a fin de evaluar el daño oxidativo inducido por la radiación gamma. Las actividades de las enzimas SOD, GPX y CAT fueron disminuidas 1 horas después (p = 0.042, p = 0.697 y p = 0.653 respectivamente) y tres horas (p = 0.003, p = 0.573 y p = 0.002 respectivamente) tras la inyección del radiofármaco. Los niveles de malondialdehido fueron aumentados 1 hora después (p = 0.10) y tres horas después (p = 0.47) de la inyección del radiofármaco. En este estudio, hallamos que la radiación a causa de la escintigrafía de perfusión miocárdica 201Tl disminuyó los niveles antioxidantes del eritrocito y aumentó los niveles de MDA

Effect of functional pinealectomy on hippocampal lipid peroxidation, antioxidant enzymes and N-methyL-D-aspartate receptor subunits 2A and 2B in young and old rats

OBJECTIVES: To investigate the effects of pinealectomy on lipid peroxidation, antioxidant status and NMDA receptor subunits 2A and 2B concentrations in hippocampus

Effects of lisinopril on NMDA receptor subunits 2A and 2B levels in the hippocampus of rats with l-NAME-induced hypertension

PubMed: 22943192Hypertension is major risk factor leading to cerebrovascular pathologies. N-methyl d-aspartate receptors (NMDARs) and renin-angiotensin system are involved in neuronal plasticity, as well as cognitive functions in the hippocampus. In this study, we examined the effects of lisinopril, an ACE inhibitor, on the levels of hippocampal NMDAR subunits; NR2A and NR2B in l-NAME (N?-nitro-l-arginine Methyl Ester)-induced hypertensive rats. In addition, malondialdehyde (MDA) levels were measured as a marker for lipid peroxidation. Compared with the control group, the MDA level was significantly increased after 8 weeks in the l-NAME-treated group. Rats treated with lisinopril and l-NAME plus lisinopril were found to have significantly decreased hippocampal MDA levels. Regarding the hippocampal concentrations of NR2A and NR2B, there were no statistically significant differences between groups. We demonstrated that lisinopril treatment has no direct regulatory effect on the levels of NR2A and NR2B in the rat hippocampus. Our results showed that Lisinopril could act as an antioxidant agent against hypertension-induced oxidative stress in rat hippocampus. The findings support that the use of lisinopril may offer a good alternative in the treatment of hypertension by reducing not only blood pressure but also prevent hypertensive complications in the brain. © 2012 Informa Healthcare USA, Inc