Can Intercropping Increase Climate Resilience of Smallholder Dryland Cropping Systems? Insights from Experimentation and Modelling

With the potential threat of more frequent climate extremes putting semi-arid crop production in jeopardy, there is a need to establish more resilient cropping systems. Intercropping is often practised by farmers in such regions, but to what extent and how can it be a viable option for future conditions? As field testing complex adaptation strategies in well-controlled environments is often difficult, we opted for a different approach: combining experimentation and modelling. A field trial was run in semi-arid India over a two-year period (2015 & 2016) in the dry season. These trials tested a split-plot designed experiment with four replicates, assessing the performance of sole versus intercropped stands, with two densities (30 cm & 60 cm between row spacing), and three drip irrigation treatments (severe stress, partial stress, and well-watered). Under low rainfall conditions, results showed that total grain yields were in-line with the irrigation treatments applied. Intercropping pearl millet led to a significantly lower total grain yield in comparison to the sole equivalent. Pearl millet achieved 1.1 t/ha when intercropped and 2.5 t/ha when grown as a sole crop. Cowpea yielded 0.8 t/ha when intercropped, and 0.8 t/ha as a sole crop. From this study, we can conclude that even when temperatures exceed 43 C crops produce reasonable yields when irrigated. In terms of pearl millet production, sole as opposed to intercrop cultivation could be more suitable. Such experiments during the dry season are arguably an opportunity for testing cropping strategies under extreme but real-world conditions. Subsequently, we used the above-described detailed data in conjunction with the agroecosystem model APSIM. Linking such experimental data with models is important to evaluate models that can be applied to explain and quantify the performance of such systems. Model performance was satisfactory and eproduced the effects of density, irrigation and year on the variables chosen. Plant height proved to be crucial for model evaluation. Simulation experiments were conducted to further evaluate plant densities, as well as genetic traits. Our combined approach is capable of improving intercropping strategies, through understanding the processes that determine interactions between specific environments and management practices

Stromal-driven and Amyloid β-dependent induction of neutrophil extracellular traps modulates tumor growth

Abstract: Tumors consist of cancer cells and a network of non-cancerous stroma. Cancer-associated fibroblasts (CAF) are known to support tumorigenesis, and are emerging as immune modulators. Neutrophils release histone-bound nuclear DNA and cytotoxic granules as extracellular traps (NET). Here we show that CAFs induce NET formation within the tumor and systemically in the blood and bone marrow. These tumor-induced NETs (t-NETs) are driven by a ROS-mediated pathway dependent on CAF-derived Amyloid β, a peptide implicated in both neurodegenerative and inflammatory disorders. Inhibition of NETosis in murine tumors skews neutrophils to an anti-tumor phenotype, preventing tumor growth; reciprocally, t-NETs enhance CAF activation. Mirroring observations in mice, CAFs are detected juxtaposed to NETs in human melanoma and pancreatic adenocarcinoma, and show elevated amyloid and β-Secretase expression which correlates with poor prognosis. In summary, we report that CAFs drive NETosis to support cancer progression, identifying Amyloid β as the protagonist and potential therapeutic target

Species difference in ANP32A underlies influenza A virus polymerase host restriction.

Influenza pandemics occur unpredictably when zoonotic influenza viruses with novel antigenicity acquire the ability to transmit amongst humans. Host range breaches are limited by incompatibilities between avian virus components and the human host. Barriers include receptor preference, virion stability and poor activity of the avian virus RNA-dependent RNA polymerase in human cells. Mutants of the heterotrimeric viral polymerase components, particularly PB2 protein, are selected during mammalian adaptation, but their mode of action is unknown. We show that a species-specific difference in host protein ANP32A accounts for the suboptimal function of avian virus polymerase in mammalian cells. Avian ANP32A possesses an additional 33 amino acids between the leucine-rich repeats and carboxy-terminal low-complexity acidic region domains. In mammalian cells, avian ANP32A rescued the suboptimal function of avian virus polymerase to levels similar to mammalian-adapted polymerase. Deletion of the avian-specific sequence from chicken ANP32A abrogated this activity, whereas its insertion into human ANP32A, or closely related ANP32B, supported avian virus polymerase function. Substitutions, such as PB2(E627K), were rapidly selected upon infection of humans with avian H5N1 or H7N9 influenza viruses, adapting the viral polymerase for the shorter mammalian ANP32A. Thus ANP32A represents an essential host partner co-opted to support influenza virus replication and is a candidate host target for novel antivirals

Modelling long term effects of cropping and managements systems on soil organic matter, C/N dynamics and crop growth

While simulation of cropping systems over a few years might reflect well the short term effects of management and cultivation, long term effects on soil properties and their consequences for crop growth and matter fluxes are not captured. Especially the effect on soil carbon sequestration/depletion is addressed by this task. Simulations of an ensemble of crop models are performed as transient runs over a period of 120 year using observed weather from three stations in Czech Republic (1961-2010) and transient long time climate change scenarios (2011-2080) from five GCM of the CMIP5 ensemble to assess the effect of different cropping and management systems on carbon sequestration, matter fluxes and crop production in an integrative way. Two cropping systems are regarded comprising two times winter wheat, silage maize, spring barley and oilseed rape. Crop rotations differ regarding their organic input from crop residues, nitrogen fertilization and implementation of catch crops. Models are applied for two soil types with different water holding capacity. Cultivation and nutrient management is adapted using management rules related to weather and soil conditions. Data of phenology and crop yield from the region of the regarded crops were provided to calibrate the models for crops of the rotations. Twelve models were calibrated in this first step. For the transient long term runs results of four models were submitted so far. Outputs are crop yields, nitrogen uptake, soil water and mineral nitrogen contents, as well as water and nitrogen fluxes to the atmosphere and groundwater. Changes in the carbon stocks and the consequences for nitrogen mineralisation, N fertilization and emissions also considered.

Modeling the multi-functionality of African savanna landscapes under global change

Various recent publications have indicated that accelerated global change and its negative impacts on terrestrial ecosystems in Southern Africa urgently demand quantitative assessment and modelling of a range of ecosystem services on which rural communities depend. Information is needed on how these Ecosystem Services (ES) can be enhanced through sustainable land management interventions and enabling policies. Yet, it has also been claimed that, to date, the required system analyses, data and tools to quantify important interactions between biophysical and socio-economic components, their resilience and ability to contribute to livelihood needs do not exist. We disagree, but acknowledge that building an appropriate integrative modelling framework for assessing the multi-functionality of savanna landscapes is challenging. Yet, in this Letter-to-the-Editor, we show that a number of suitable modelling components and required data already exist and can be mobilized and integrated with emerging data and tools to provide answers to problem-driven questions posed by stakeholders on land management and policy issues.German Federal Ministry of Education and Researchhttps://onlinelibrary.wiley.com/journal/1099145xhj2022Zoology and Entomolog

The genetic architecture of the human cerebral cortex

The cerebral cortex underlies our complex cognitive capabilities, yet little is known about the specific genetic loci that influence human cortical structure. To identify genetic variants that affect cortical structure, we conducted a genome-wide association meta-analysis of brain magnetic resonance imaging data from 51,665 individuals. We analyzed the surface area and average thickness of the whole cortex and 34 regions with known functional specializations. We identified 199 significant loci and found significant enrichment for loci influencing total surface area within regulatory elements that are active during prenatal cortical development, supporting the radial unit hypothesis. Loci that affect regional surface area cluster near genes in Wnt signaling pathways, which influence progenitor expansion and areal identity. Variation in cortical structure is genetically correlated with cognitive function, Parkinson's disease, insomnia, depression, neuroticism, and attention deficit hyperactivity disorder

Effects of Anacetrapib in Patients with Atherosclerotic Vascular Disease

BACKGROUND: Patients with atherosclerotic vascular disease remain at high risk for cardiovascular events despite effective statin-based treatment of low-density lipoprotein (LDL) cholesterol levels. The inhibition of cholesteryl ester transfer protein (CETP) by anacetrapib reduces LDL cholesterol levels and increases high-density lipoprotein (HDL) cholesterol levels. However, trials of other CETP inhibitors have shown neutral or adverse effects on cardiovascular outcomes. METHODS: We conducted a randomized, double-blind, placebo-controlled trial involving 30,449 adults with atherosclerotic vascular disease who were receiving intensive atorvastatin therapy and who had a mean LDL cholesterol level of 61 mg per deciliter (1.58 mmol per liter), a mean non-HDL cholesterol level of 92 mg per deciliter (2.38 mmol per liter), and a mean HDL cholesterol level of 40 mg per deciliter (1.03 mmol per liter). The patients were assigned to receive either 100 mg of anacetrapib once daily (15,225 patients) or matching placebo (15,224 patients). The primary outcome was the first major coronary event, a composite of coronary death, myocardial infarction, or coronary revascularization. RESULTS: During the median follow-up period of 4.1 years, the primary outcome occurred in significantly fewer patients in the anacetrapib group than in the placebo group (1640 of 15,225 patients [10.8%] vs. 1803 of 15,224 patients [11.8%]; rate ratio, 0.91; 95% confidence interval, 0.85 to 0.97; P=0.004). The relative difference in risk was similar across multiple prespecified subgroups. At the trial midpoint, the mean level of HDL cholesterol was higher by 43 mg per deciliter (1.12 mmol per liter) in the anacetrapib group than in the placebo group (a relative difference of 104%), and the mean level of non-HDL cholesterol was lower by 17 mg per deciliter (0.44 mmol per liter), a relative difference of -18%. There were no significant between-group differences in the risk of death, cancer, or other serious adverse events. CONCLUSIONS: Among patients with atherosclerotic vascular disease who were receiving intensive statin therapy, the use of anacetrapib resulted in a lower incidence of major coronary events than the use of placebo. (Funded by Merck and others; Current Controlled Trials number, ISRCTN48678192 ; ClinicalTrials.gov number, NCT01252953 ; and EudraCT number, 2010-023467-18 .)

A novel approach to tele-echocardiography across the Pacific.

Telecardiology provides remote delayed interpretation of echocardiographic images through a store and forward program between the interpreting center, Tripler Army Medical Center, Honolulu Hawaii, and the image acquisition center, Guam Naval Hospital, Guam USA. This routine store and forward system has inherent delay, limiting application for management of acute medical conditions. In this case report we describe a novel methodology for real-time echocardiograpic interpretation methodology integrated methodology with the eICU system (VISICU Inc., Baltimore MD). This case report demonstrates the feasibility of a clinically relevant remote real-time echocardiographic interpretation strategy, utilizing commonly available equipment