From invasion to fish fodder: inclusion of the brown algae rugulopteryx okamurae in aquafeeds for european sea bass dicentrarchus labrax (L., 1758)

In keeping with the premises of Blue Circular Economy in the European Union, the present study explored the possibility of using the invasive brown alga Rugulopteryx okamurae in aquafeeds for European sea bass (Dicen-trarchus labrax). Assuming the raw algae biomass could negatively impact animal performance, four experi-mental formulations were prepared, by including macroalgae material at 5%, using crude (CR), enzymatically hydrolysed and fermented (EF), enzymatically hydrolysed (E), or fermented (F) R. okamurae biomass, which we tested against a control feed (CT). To evaluate the effects of the experimental diets, besides animal growth performance and biometric parameters, we devised a toolbox focused on the intestine and intestinal function: i) ex-vivo epithelial resistance and permeability in Ussing chambers; ii) microbiota composition through NGS; iii) expression profiles of selected markers for epithelial integrity, transport, metabolism, and immune response, by qPCR. Our results show differentiated allometric growth among diets, coupled with intestinal epithelium al-terations in permeability, integrity, and amino acid transport. Additionally, evidence of microbiota dysbiosis and contrasting immune responses between experimental diets, i.e. pro-inflammatory vs. anti-inflammatory, are also described. In conclusion, we believe that R. okamurae could be a suitable resource for aquafeeds for the European sea bass, although its use requires a pre-treatment before inclusion. Otherwise, while the fish still have a positive growth performance, the gastrointestinal tract pays a toll on the integrity, transport, and inflammatory processes.LA/P/0101/2020info:eu-repo/semantics/publishedVersio

Effect of selenium application on mineral macro- and micronutrients and antioxidant status in strawberries

The application of selenium (Se) as sodium selenite (Na2SeO3) at 0, 2, and 4 mg L-1 concentrations in nutrient solution to strawberry plants was evaluated. Selenium did not modify the dry weights of the roots, stems, leaves, and fruits, or the fresh weights of the stems and fruits. The 4 mg L-1 concentration caused decreases in the fresh weights of the roots and leaves and in the yield. The mineral content of different plant organs changed but was not adversely affected by Se applications, with the 2 mg L-1 treatment having a lower impact on mineral concentration variation, as well as temporary positive effects on the fruits’ antioxidant status. The fruit pH was not adversely affected by application of Se. Se application in nutrient solution proved to be an adequate technique to increase the Se content in all plant organs. Se concentration exhibited a differential distribution, with the highest levels in the roots, followed by the leaves and crowns; the fruits had the lowest levels, reaching an average concentration of 31.2 mg kg-1 of dry weight. By contrast, fruits from the untreated plants obtained an average concentration of only 6.35 mg kg-1, with no decreases in the concentrations of other mineral elements in treated plants

Low dietary inclusion of nutraceuticals from microalgae improves feed efficiency and modifies intermediary metabolisms in gilthead sea bream (Sparus aurata)

The aim of this work was to evaluate two functional feeds for the gilthead seabream, Sparus aurata, containing low inclusion of two microalgae-based products (LB-GREENboost, LBGb; and LB-GUThealth, LBGh). Fish (12-13 g) were fed for 13 weeks a control diet or one of the four diets supplemented with both products at 0.5% or 1%. LBGb and LBGh did not affect specific growth rate or survival, but increased feed efficiency by decreasing feed intake and enlarging the intestines. LBGb increased hepatosomatic index and reduced cortisol levels in plasma, while both products lowered plasma lactate. Extensive metabolite and metabolic enzyme profiling revealed that microalgae supplementations, especially 1% LBGh: (i) decrease plasma lactate and increase hepatic glycogen, (ii) reduce hepatic gluconeogenesis, (iii) enhance hepatic lipogenic activity and lipid secretion, (iv) led fish to double triglyceride content in muscle and to stimulate its lipid oxidative capacity, and (v) increase the content of monounsaturated fatty acids and the omega-3 alpha-linolenic acid in muscle. This study demonstrates that both microalgae-based products are suited to improve feed efficiency and orchestrate significant changes in the intermediary metabolism in gilthead seabream juveniles

Asignaturas de dobles grados

El objeto del presente estudio es transmitir nuestra experiencia en un fenómeno que aparece cada vez con mayor frecuencia en la universidad española, y que, con la puesta en marcha del Espacio Europeo de Educación Superior y su metodología, cuenta con más relevancia. Nos referimos a los dobles grados. Los dobles grados buscan ofrecer estudios más atractivos, tanto a los nuevos y futuros estudiantes como a los futuros empleadores y demandantes de esa mano de obra especialmente cualificada: las empresas. En el desarrollo de este artículo, abordaremos la casuística existente en este departamento con la participación de los distintos docentes que participan tanto en la docencia de las asignaturas denominadas “tradicionales” que son las que se imparten en la mayoría de los grados, sus experiencias docentes y el perfil de alumnado que participa en sus clases, junto con aquellos docentes que se enfrentan a las asignaturas denominadas de “dobles grados” y las características del alumnado, aspecto este último que está en función del tipo de doble grado, y de la especialización que persigue el alumno

Perfiles de valores interpersonales y análisis de conductas y actitudes sociales de adolescentes

The aims of this study are to identify the interpersonal value profiles and analyze whether there are differences with respect to behavior and social attitude in a sample made up of 885 Compulsory Secondary Education (ESO) students (14-17 years), of whom 49.8% (n = 441) were males and 50.2% (n = 444) were females. The results show the identification of three different interpersonal value profiles corresponding to different levels of antisocial and criminal behavior. Thus the ARL-BCB (high recognition and leadership – low conformity and benevolence) group had the most antisocial and criminal behavior, low levels of social sensitivity, conformity and helpfulness-collaboration, and high levels of dominance, aggressiveness-stubbornness and apathy-isolation. Points that need to be worked on in preventive intervention of antisocial and criminal behavior of students by improving their social attitudes are highlighted.; Los objetivos de este trabajo son identificar los diferentes perfiles de valores interpersonales y analizar si entre ellos, existen diferencias respecto a la conducta y actitud social. Todo ello, en una muestra formada por 885 alumnos de Educación Secundaria (14 a 17 años), de los cuales, el 49.8% (n= 441) son hombres y el 50.2% (n = 444) mujeres. Los resultados muestran la identificación de tres perfiles de valores interpersonales diferentes, correspondiéndose con diferentes niveles de conductas delictivas y antisociales. Así, el grupo con mayor presencia de ambas conductas, antisociales y delictivas, bajos niveles de sensibilidad social, conformidad y ayuda- colaboración, y altos niveles de dominancia, agresividad-terquedad y apatía-retraimiento es el ARL-BCB (alto reconocimiento y liderazgo -baja conformidad y benevolencia-). Finalmente, se ponen de relieve los aspectos a trabajar en la intervención preventiva de las conductas antisociales y delictivas del alumnado a través de la mejora de las actitudes sociales

Acceptance of living liver donation among medical students: A multicenter stratified study from Spain

AIM: To analyze the attitude of Spanish medical students toward living liver donation (LLD) and to establish which factors have an influence on this attitude. METHODS: STUDY TYPE: A sociological, interdisciplinary, multicenter and observational study. STUDY POPULATION: Medical students enrolled in Spain (n = 34000) in the university academic year 2010-2011. SAMPLE SIZE: A sample of 9598 students stratified by geographical area and academic year. Instrument used to measure attitude: A validated questionnaire (PCID-DVH RIOS) was self-administered and completed anonymously. Data collection procedure: Randomly selected medical schools. The questionnaire was applied to each academic year at compulsory sessions. STATISTICAL ANALYSIS: Student´s t test, ?(2) test and logistic regression analysis. RESULTS: The completion rate was 95.7% (n = 9275). 89% (n = 8258) were in favor of related LLD, and 32% (n = 2937) supported unrelated LLD. The following variables were associated with having a more favorable attitude: (1) age (P = 0.008); (2) sex (P < 0.001); (3) academic year (P < 0.001); (4) geographical area (P = 0.013); (5) believing in the possibility of needing a transplant oneself in the future (P < 0.001); (6) attitude toward deceased donation (P < 0.001); (7) attitude toward living kidney donation (P < 0.001); (8) acceptance of a donated liver segment from a family member if one were needed (P < 0.001); (9) having discussed the subject with one's family (P < 0.001) and friends (P < 0.001); (10) a partner's opinion about the subject (P < 0.001); (11) carrying out activities of an altruistic nature; and (12) fear of the possible mutilation of the body after donation (P < 0.001). CONCLUSION: Spanish medical students have a favorable attitude toward LLD

Catálogo de las aves de la Región de Murcia (España)

La avifauna constituye uno de los componentes fundamentales y de mayor interés de conservación de la biodiversidad de la Región de Murcia (España). El catálogo que se presenta consta de un total de 339 especies agrupadas en 69 familias y 24 órdenes, de las cuales 78 se consideran principalmente invernantes, 51 migradoras, 41 estivales, 85 residentes, 82 ocasionales y 2 son exóticas naturalizadas. Para cada especie se describe sucintamente su estatus, abundancia y distribución en el territorio regional. Se aporta además un listado de 31 especies exóticas no naturalizadas. En comparación con el anterior catálogo de las aves murcianas, elaborado hace 30 años, el presente catálogo incorpora 71 nuevas especies, sin considerar las exóticas. Se discuten las posibles causas de este incremento, en el contexto de un escenario de cambio global, así como los cambios más significativos en la ornitofauna murciana. También se comentan los numerosos cambios producidos en relación con aspectos taxonómicos y la ordenación sistemática de especies

FabLab en Educación: propuesta formativa y práctica para la creación de recursos didácticos dirigida a la docencia del profesorado universitario

El proyecto de innovación docente FabLab (laboratorios de fabricación digital) en educación pretende dotar al profesorado de una serie de competencias tecnológicas y pedagógicas que le permita ofrecer al alumnado una formación mucho más rica, integradora de contenidos, inclusiva, y que facilite el empoderamiento del alumnado para el emprendimiento profesional. El objetivo principal del proyecto es promover un cambio pedagógico en el profesorado para que sea capaz de adaptarse a las exigencias de una sociedad cada vez más tecnológica y en la que se demanda unos tipos de competencias que claramente no pueden adquirirse en la Universidad si no se tiene acceso, mínimamente, a unos recursos y dispositivos tecnológicos. En este sentido, la creación del FabLab permite crear un espacio creativo y productivo relacionado con la tecnología que favorece el acceso a ella y la adquisición de unas competencias tecnológicas y didácticas que, por la salida profesional del alumnado, permitiría acercar las materias denominadas STEM a las chicas de las etapas educativas no universitarias para que continúen estudios superiores relacionadas con estas asignaturas. El proyecto se articula en torno a cuatro fases: formación y sensibilización, diseño y desarrollo de materiales docentes, implementación en el estudiantado y la evaluación. Para el desarrollo del mismo se ha necesitado dos cursos académicos en los que el profesorado ha recibido una formación tanto virtual como presencial por diferentes especialistas. Para la formación y asesoramiento se ha contado con dos profesores de centros de secundaria de Granada que ya manejan algunos de los recursos y dispositivos tecnológicos en sus aulas. Además, han recibido formación sobre la gamificación de la docencia y el diseño de insignias para cuando se las tengan que otorgar al alumnado. Se han conseguido realizar diversos proyectos tecnológicos utilizando la placa Arduino y los diferentes componentes y accesorios como, por ejemplo, un vehículo sigue líneas con sensores de proximidad y un ascensor de vehículos automatizado al detectar el vehículo en el interior. También se ha elaborado un material digital con Inkscape, GIMP y Tinkercad para la para la impresión en 3D de ficha para la gamificación de diversos juegos elaborados para el aprendizaje de, por ejemplo, los mitos griegos. Para la docencia habitual, la formación recibida, así como los dispositivos utilizados y los materiales realizados, son un elemento innovador que les permite al profesorado ofrecer unos recursos atractivos para los aprendizajes de los contenidos de las diferentes materias que imparten.The FabLab (digital fabrication laboratories) teaching innovation project in education aims to provide teachers with a series of technological and pedagogical skills that will enable them to offer students a much richer, more inclusive and inclusive education that integrates content and facilitates the empowerment of students for professional entrepreneurship. The main objective of the project is to promote a pedagogical change in the teaching staff so that they are able to adapt to the demands of an increasingly technological society that demands certain types of skills that clearly cannot be acquired at the University if they do not have access, minimally, to technological resources and devices. In this sense, the creation of the FabLab makes it possible to create a creative and productive space related to technology that favours access to it and the acquisition of technological and didactic skills that, due to the students' professional opportunities, would bring STEM subjects closer to girls in non-university educational stages so that they can continue higher studies related to these subjects. The project is structured around four phases: training and awareness-raising, design and development of teaching materials, implementation among students and evaluation. It has taken two academic years to develop the project, during which the teaching staff have received both virtual and face-to-face training from different specialists. Two teachers from secondary schools in Granada, who already use some of the technological resources and devices in their classrooms, have been involved in the training and assessment. In addition, they have received training on the gamification of teaching and the design of badges for when they have to award them to students. They have managed to carry out various technological projects using the Arduino board and the different components and accessories, such as a vehicle that follows lines with proximity sensors and an automated vehicle lift when it detects the vehicle inside. Digital material has also been developed with Inkscape, GIMP and Tinkercad for the 3D printing of cards for the gamification of various games developed for the learning of, for example, Greek myths. For regular teaching, the training received, as well as the devices used and the materials produced, are an innovative element that allows teachers to offer attractive resources for learning the contents of the different subjects they teach.Unidad de Calidad, Innovación Docente y Prospectiva de la Universidad de Granad

Transmitted drug resistance to antiretroviral drugs in Spain during the period 2019–2021

To evaluate the prevalence of transmitted drug resistance (TDR) to nucleoside and nonnucleoside reverse transcriptase inhibitors (NRTI, NNRTI), protease inhibitors (PI), and integrase strand transfer inhibitors (INSTI) in Spain during the period 2019-2021, as well as to evaluate transmitted clinically relevant resistance (TCRR) to antiretroviral drugs. Reverse transcriptase (RT), protease (Pro), and Integrase (IN) sequences from 1824 PLWH (people living with HIV) were studied. To evaluate TDR we investigated the prevalence of surveillance drug resistance mutations (SDRM). To evaluate TCRR (any resistance level >= 3), and for HIV subtyping we used the Stanford v.9.4.1 HIVDB Algorithm and an in-depth phylogenetic analysis. The prevalence of NRTI SDRMs was 3.8% (95% CI, 2.8%-4.6%), 6.1% (95% CI, 5.0%-7.3%) for NNRTI, 0.9% (95% CI, 0.5%-1.4%) for PI, and 0.2% (95% CI, 0.0%-0.9%) for INSTI. The prevalence of TCRR to NRTI was 2.1% (95% CI, 1.5%-2.9%), 11.8% for NNRTI, (95% CI, 10.3%-13.5%), 0.2% (95% CI, 0.1%-0.6%) for PI, and 2.5% (95% CI, 1.5%-4.1%) for INSTI. Most of the patients were infected by subtype B (79.8%), while the majority of non-Bs were CRF02_AG (n = 109, 6%). The prevalence of INSTI and PI resistance in Spain during the period 2019-2021 is low, while NRTI resistance is moderate, and NNRTI resistance is the highest. Our results support the use of integrase inhibitors as first-line treatment in Spain. Our findings highlight the importance of ongoing surveillance of TDR to antiretroviral drugs in PLWH particularly with regard to first-line antiretroviral therapy