Preparation and characterization of yttria stabilized zirconia (YSZ) films deposited by dip-coating on LSM-YSZ substrate with different proportions

In this work, sol-gel/dip-coating process for obtaining yttria stabilized zirconia (YSZ, ZrO2–8% Y2O3) films deposited onto LSM-YSZ (lanthanum strontium manganite (LSM, La0.7Sr0.3MnO3) mixed with YSZ) with different proportions (20/80, 50/50, 80/20) was investigated. The films were deposited on substrate varying the number of layers deposited. LSM powders were obtained by the combustion method using metal nitrates and urea and YSZ was commercial. LSM-YSZ composite powders were obtained by the solid state method, through ball-milling of a mixture of LSM-YSZ (mass ratio) powder for 4 h with 500 rpm using ZrO2 balls as milling media. For LSMYSZ substrate a study of sintering temperature was performed, where the optimum sintering temperature for each LSM-YSZ studied proportion was obtained. According to our results, it was found that 1100 ºC is the optimum sintering temperature for 20/80, 950 ºC is the optimum sintering temperature for 50/50 and 900 ºC is the optimum sintering temperature for 80/20 because of the good phase formation. The films were characterized by X-ray Diffraction (XRD) and Scanning Electron Microscopy (SEM). The films showed LSM, YSZ, SrZrO3 and La2O3 phases. Crack-free, homogeneous and well adhered films were obtained with a thickness between 3 and 38 μm. In this work, YSZ films with appropriate thickness were obtained for application as SOFC electrolyte

REPRODUÇÃO FEMININA E SAÚDE SOB OS OLHARES DE MULHERES SEM FILHOS

RESUMO Trata-se de estudo qualitativo, na metodologia história oral temática, fundamentado na sociologia compreensiva do cotidiano, que tem por objetivo compreender a influência da reprodução feminina na construção da identidade de mulheres sem filhos, considerando o olhar sociopolítico da saúde sobre o corpo feminino. Participaram 19 mulheres sem filhos, maiores de 18 anos, em idade reprodutiva nas décadas de 1950 a 2010, que conheçam ou tenham sido atendidas pelas políticas públicas de saúde. Foram escolhidas pela técnica de snowball. A coleta de dados foi feita por meio de entrevistas semiestruturadas. Utilizou-se a análise de narrativas para tratamento dos dados. Os resultados apresentam uma mudança no papel feminino e no ideal de mulher-mãe. O corpo feminino assume novas formas de subjetivação, dissociando reprodução da sexualidade, e a maternidade aparece como uma escolha. Essas mudanças exigem a implementação ou efetivação de políticas públicas Já existentes que atendam às mulheres em outras perspectivas que não apenas nos aspectos reprodutivos ou ciclo gravídico-puerperal

O tratamento sucessório dado ao cônjuge e ao companheiro à luz do princípio da isonomia

No presente artigo, abordar-se-á a situação do companheiro no direito sucessório, bem como uma possível inconstitucionalidade do tratamento dado ao mesmo, o que feriria o princípio da isonomia. O Código Civil de 2002, bem como as demais legislações aplicáveis à espécie, trouxe algumas inovações e transformações na seara do direito sucessório, contudo, não deu à união estável o mesmo valor jurídico do casamento. Neste sentido, será analisado o tratamento dado ao companheiro no direito sucessório, para tanto, o método abordado será basicamente a análise bibliográfica, que hoje conta com vasto acervo, visto que o tema é contemporâneo

An engineered protein antagonist of K-Ras/B-Raf interaction

Ras is at the hub of signal transduction pathways controlling cell proliferation and survival. Its mutants, present in about 30% of human cancers, are major drivers of oncogenesis and render tumors unresponsive to standard therapies. Here we report the engineering of a protein scaffold for preferential binding to K-Ras G12D. This is the first reported inhibitor to achieve nanomolar affinity while exhibiting specificity for mutant over wild type (WT) K-Ras. Crystal structures of the protein R11.1.6 in complex with K-Ras WT and K-Ras G12D offer insight into the structural basis for specificity, highlighting differences in the switch I conformation as the major defining element in the higher affinity interaction. R11.1.6 directly blocks interaction with Raf and reduces signaling through the Raf/MEK/ERK pathway. Our results support greater consideration of the state of switch I and provide a novel tool to study Ras biology. Most importantly, this work makes an unprecedented contribution to Ras research in inhibitor development strategy by revealing details of a targetable binding surface. Unlike the polar interfaces found for Ras/effector interactions, the K-Ras/R11.1.6 complex reveals an extensive hydrophobic interface that can serve as a template to advance the development of high affinity, non-covalent inhibitors of K-Ras oncogenic mutants.National Institutes of Health (U.S.) (Grant 5-R01-CA096504-15

PARASITOS EM RÉPTEIS DO NÚCLEO DE CONSERVAÇÃO E REABILITAÇÃO DE ANIMAIS SILVESTRES DE UMA UNIVERSIDADE PÚBLICA-BRASIL

The medical clinic for reptiles has grown in recent years, whether for attending free-living animals in rehabilitation centers or for pets. The knowledge of the parasitic fauna of the Reptilia Class is important for the identification of pathogenic agents and effective therapy. Fecal samples were collected from 35 animals of the Orders Crocodylia (N=3), Squamata (N=3) and Testudines (N=29) attended from 2013 to 2018 at the Center for Conservation and Rehabilitation of Wild Animals (PRESERVAS) of the Universidade Federal do Rio Grande do Sul (UFRGS). Fecal samples were processed by the methods of Willis, Lutz and Sheather at the Laboratory of Helminthology at UFRGS. The percentage of positive samples was 17.14% (6/35): three Trachemys dorbogni (Duméril & Bibron 1835) presenting Serpinema spp., Physaloptera spp. and Strongyloidea, respectively; two Caiman latirostris (Daudin, 1802) with Strongyloides spp. and Augusticaecum spp., respectively, and Iguana iguana (Linnaeus, 1758) with Eimeria spp. This study expands the list of parasites in reptiles from the state of Rio Grande do Sul.La clínica médica para reptiles ha crecido en los últimos años, ya sea para atender animales de vida libre en centros de rehabilitación o para mascotas. El conocimiento de la fauna parasitaria de la clase Reptilia es importante para la identificación de agentes patógenos y una terapia eficaz. Se recolectaron muestras fecales de 35 animales de los Órdenes Crocodylia (N = 3), Squamata (N = 3) y Testudines (N = 29) atendidos de 2013 a 2018 en el Centro de Conservación y Rehabilitación de Animales Silvestres (PRESERVAS) de la Universidade Federal do Rio Grande do Sul (UFRGS). Las muestras fecales fueron procesadas por los métodos de Willis, Lutz y Sheather en el Laboratorio de Helmintología de la UFRGS. El porcentaje de muestras positivas fue del 17,14% (6/35): tres Trachemys dorbogni (Duméril & Bibron 1835) presentaron Serpinema spp., Physaloptera spp. y Strongyloidea, respectivamente; dos Caiman latirostris (Daudin, 1802) con Strongyloides spp. y Augusticaecum spp., respectivamente, e Iguana iguana (Linnaeus, 1758) con Eimeria spp. Este estudio amplía la lista de parásitos en reptiles del estado de Rio Grande do Sul.A clínica médica de répteis tem crescido nos últimos anos, seja pelo atendimento de animais de vida livre em centros de reabilitação ou pela aquisição como pets. O conhecimento da fauna parasitária da Classe Reptilia é importante para identificação de agentes patogênicos e terapêutica efetiva. Foram coletadas amostras fecais de 35 animais das Ordens Crocodylia (N= 3), Squamata (N=3) e Testudines (N=29) atendidos de 2013 à 2018 no Núcleo de Conservação e Reabilitação de Animais Silvestres (PRESERVAS) da Universidade Federal do Rio Grande do Sul (UFRGS). Amostras fecais foram processadas pelos métodos de Willis, Lutz e Sheather no Laboratório de Helmintologia da UFRGS. A porcentagem de amostras positivas foi de 17, 14 % (6/35): três Trachemys dorbogni (Duméril & Bibron 1835) apresentando Serpinema spp., Physaloptera spp. e Strongyloidea, respectivamente; dois Caiman latirostris (Daudin, 1802) com Strongyloides spp. e Augusticaecum spp., respectivamente é uma Iguana iguana (Linnaeus, 1758) com Eimeria spp. Este estudo amplia a lista de parasitos em répteis do estado do Rio Grande do Sul

CHILD DEVELOPMENT: COMPARISON BETWEEN CHILDREN WHO ATTEND OR DO NOT ATTEND PUBLIC DAYCARE CENTRES

Objective: To avaluate and compare the development of children attending public nursery school from their peers who remain only in the home environment. Methods: We performed an observational, cross-sectional study with 167 children aged between 11 and 57 months from Alto Vale do Jequitinhonha, Minas Gerais, Brazil. The subjects were divided into two groups (nursery and hom areas:e), homogeneous in age, gender, socioeconomic status, and maternal education. The development areas: personal-social, language, and fine and gross motor were evaluated through the Denver II test and the quality of environment of the five public nursery school ‘ through the Infant/Toddler Environment Rating Scale-Revised Edition (ITERS-R). We used the chi-square statistical test for comparison between groups. Results: There were no significant statistical differences between groups for the overall test result for Denver II, nor for the different areas of the test. The environments presented daycare quality between ‘inadequate’ and ‘minimal’. Thus, there was no difference between attending a low quality daycare or just staying in the home for child development. Conclusion: No difference was observed when comparing the development areas: personal-social, language, and gross and fine motor of child who attend public nursery school and their peers who remained only in the home environment. The daycare environments analysed showed quality considered ‘inadequate’, which may have influenced the results as the literature has pointed out the need for a quality childcare environment to positively influence child development

Prova de Trabalho de Parto Após uma Cesárea Anterior

Objetivo: estudar a validade da prova de trabalho de parto (PTP) em gestantes com uma cesárea anterior. Métodos: estudo retrospectivo, tipo coorte, incluindo 438 gestantes com uma cesárea anterior ao parto em estudo e seus 450 recém-nascidos (RN), divididas em dois grupos - com e sem PTP. O tamanho amostral mínimo foi de 121 gestantes/grupo. Considerou-se variável independente a PTP e as dependentes relacionaram-se à ocorrência de parto vaginal e à freqüência de complicações maternas e perinatais. Foram efetuadas análises uni e multivariada, respectivamente. A comparação entre as freqüências (%) foi analisada pelo teste do qui-quadrado (chi²) com significância de 5% e regressão logística com cálculo do odds ratio (OR) e do intervalo de confiança a 95% (IC95%). Resultados: a PTP associou-se a 59,2% de partos vaginais. Foi menos indicada nas gestantes com mais de 40 anos (2,7% vs 6,5%) e nas portadoras de doenças associadas e complicações da gravidez: síndromes hipertensivas (7,0%) e hemorragias de 3º trimestre (0,3%). A PTP não se relacionou às complicações maternas e perinatais. As gestantes que tiveram o parto por cesárea, independente da PTP, apresentaram maior risco de complicações puerperais (OR = 3,53; IC95% = 1,57-7,93). A taxa de mortalidade perinatal foi dependente do peso do RN e das malformações fetais e não se relacionou à PTP. Ao contrário, as complicações respiratórias foram mais freqüentes nos RN de mães não testadas quanto à PTP (OR = 1,92; IC95% = 1,20-3,07). Conclusões: os resultados comprovaram que a PTP em gestantes com uma cesárea anterior é estratégia segura - favoreceu o parto vaginal em 59,2% dos casos e não interferiu com a morbimortalidade materna e perinatal. Portanto, é recurso que deve ser estimulado.Purpose: to study trial of labor (TOL) for vaginal birth after one previous cesarean section. Methods: this is a retrospective cohort study that included 438 pregnant women with one previous cesarean section and their 450 newborns. They were divided into two groups - with and without TOL. The minimum sample size was 121 pregnant mothers per group. TOL was considered as an independent variable and vaginal birth and maternal and perinatal complication frequency as dependent variables. Both univariate and multivariate analyses were performed. The comparison of observed frequencies (%) was analyzed by the chi-squared test (chi²) with 5% significance, and linear regression from the odds ratio (OR) and confidence interval of 95% (CI95%). Results: TOL was used in 59.2% of vaginal deliveries. It was less used in women over 40 years (2.7% vs 6.7%) and in those with clinical or obstetrical diseases such as arterial hypertension (7.0%) and bleeding in the third trimester (0.3%). There was a higher risk for puerperal complications with cesarean deliveries (OR = 3.53, CI 95% = 1.57-7.93), independent of TOL. Perinatal mortality was dependent on neonatal weight and fetal malformations, not on TOL. Newborns from mothers not submitted to TOL were at a higher risk for developing breathing complications (OR = 1.92 CI 95% = 1.20-3.07). Conclusions: The results confirm that trial of labor after a previous cesarean section is a safe method - assisting vaginal delivery in 59.2% of births and not interfering with maternal and perinatal mortality. It is a treatment that should be stimulated

The use of radiotherapy in the treatment of oral cancer / O uso da radioterapia no tratamento do câncer oral

Most of the development of neoplasms has epithelial origin, and the most frequent for oral cancer is squamous cell carcinoma (SCC), of which one should be aware of the extrinsic and intrinsic factors that may be relevant in the development of precancerous lesions. The diagnosis of this type of cancer is made with clinical, histopathological and imaging exams. Thus, this ressearch was carried out with the objetive to address the treatment of oral cancer through radiotherapy, identifying different techniques for the use of radiation and establishing measures that help in the control of radiation beams in the face of the exposed pathology. This study had as source articles published in the electronic databases LILACS and PubMed, between 1996 and 2020 through the descriptors: “radiotherapy", "oral cancer”. Radiotherapy for oral cancer can be performed in an adjuvant or neoadjuvant way, providing radiation by teletherapy through techniques known as Conventional Radiotherapy, Conformational Radiotherapy, and Intensity-Modulated Radiation Therapy. Although radiotherapy is capable to eliminate tumor cells, abnormalities induced by radiation may appear inside the oral cavity, and these abnormalities can be minimized by controlling radiation doses distribution in addition to significant protection of adjacent healthy tissues through the use of intraoral devices, contributing to the improvement of the patient's quality of life. 

Consortium neuroscience of attention deficit/hyperactivity disorder and autism spectrum disorder:The ENIGMA adventure

International audienc

The IASLC Early Lung Imaging Confederation (ELIC) Open-Source Deep Learning and Quantitative Measurement Initiative.

BackgroundWith global adoption of CT lung cancer screening, there is increasing interest to use artificial intelligence (AI) deep learning methods to improve the clinical management process. To enable AI research using an open source, cloud-based, globally distributed, screening CT imaging dataset and computational environment that are compliant with the most stringent international privacy regulations that also protects the intellectual properties of researchers, the International Association of the Study of Lung Cancer (IASLC) sponsored development of the Early Lung Imaging Confederation (ELIC) resource in 2018. The objective of this report is to describe the updated capabilities of ELIC and illustrate how this resource can be utilized for clinically relevant AI research.MethodsIn this second Phase of the initiative, metadata and screening CT scans from two time points were collected from 100 screening participants in seven countries. An automated deep learning AI lung segmentation algorithm, automated quantitative emphysema metrics, and a quantitative lung nodule volume measurement algorithm were run on these scans.ResultsA total of 1,394 CTs were collected from 697 participants. The LAV950 quantitative emphysema metric was found to be potentially useful in distinguishing lung cancer from benign cases using a combined slice thickness ≥ 2.5 mm. Lung nodule volume change measurements had better sensitivity and specificity for classifying malignant from benign lung nodules when applied to solid lung nodules from high quality CT scans.ConclusionThese initial experiments demonstrated that ELIC can support deep learning AI and quantitative imaging analyses on diverse and globally distributed cloud-based datasets