Defoliation responses of different grapevine cultivars to postharvest ethephon treatments

The defoliation responses of several grapevine cultivars to postharvest ethephon sprays at 0, 500, 2000, 5000 ppm were observed. Sprays were applied at the beginning of October to vines of Barbera, Carignane, Emerald Seedless, Ribier, Ruby Seedless, Thompson Seedless and Flame Tokay cultivars.The most effective concentration was 5000 ppm. The defoliation response varied greatly among the varieties. Barbera and Carignane were almost completely defoliated 20-30 d after treatment, whereas Flame Tokay showed no response. However, high concentrations of ethephon resulted in a considerable delay in shoot development the following spring.Défoliation induite en différents cépages par un traitement avec de l'éthéphon après la vendangeLa défoliation causée par des traitements d'éthephon (0, 500, 2000, 5000 ppm) a été étudiée sur différents cépages (V. vinifera).Les applications ont été faites le 10 octobre sur les cépages à raisin de table et de cuve ci-après: Barbera, Carignane; Emerald Seedless, Ribier, Ruby Seedless, Thompson Seedless et Flame Tokay. La concentration de 5000 ppm se révéla la plus efficace. La défoliation des plantes traitées a été très différente selon les cépages: Barbera et Carignane par exemple ont été presque complètement privés des feuilles après 20-30 d du traitement, tandis que le Flame Tokay ne donna qu'une très faible réponse.Il faut signaler en tout cas que les concentrations les plus fortes d'éthéphon ont causé un retard considerable dans le débourrement du printemps suivant

Effect of vine vigour of Vitis vinifera cv. Nebbiolo clones on wine acidity and quality

The grapevine cv. Nebbiolo grown in northern Italy produces high-quality red wines, of which Barolo and Barbaresco are the best known. During a clonal selection project, clones of this variety were assessed for their agronomical and enological value. Different degrees of vegetative vigour were found among them, and this was related to modifications of must and wine composition, with particular respect to the acidity. Over 4 years of observations, vigorous clones produced musts and wines of higher pH, regardless of the amount of titratable acidity. This was associated with a higher malic acid content in the juice and with a higher concentration of potassium in the wine. In addition, wines from vigorous clones showed an unbalanced ratio of colour components. They ranked at the lowest score in the sensory evaluation tests

Modifications in Chemical, Physical and Mechanical Properties of Nebbiolo (Vitis vinifera L.) Grape Berries Induced by Mixed Virus Infection

Modifications in grape quality parameters induced by mixed infection with GFLV and GFkV, GLRaV-1and GVA, and GLRaV-3 and GVA in three Nebbiolo clones were compared against healthy plants of thesame clones in two experimental vineyards in Piemonte, northwest Italy. The aim of the study was toevaluate the effect of virus infection on the mechanical properties of the berry skin and the whole berry asassessed by texture analysis tests, and on the amount and quality of berry skin phenols. Differences wereobserved in grapevine vigour, yield and juice composition, depending on the viral status of the plants. Theanthocyanin profile of the vines infected with GFV and GFkV and those infected with GLRaV-1 and GVAshowed a lower percentage of the more stable tri-substituted malvidin-3-glucoside and a higher percentageof cyanidin and peonidin-3-glucosides. Texture analysis showed that the viruses may increase berry-skinthickness and reduce phenol extractability. These effects carry practical implications for wine quality

The induction of α-helical structure in partially unfolded HypF-N does not affect its aggregation propensity

The conversion of proteins into structured fibrillar aggregates is a central problem in protein chemistry, biotechnology, biology and medicine. It is generally accepted that aggregation takes place from partially structured states of proteins. However, the role of the residual structure present in such conformational states is not yet understood. In particular, it is not yet clear as to whether the α-helical structure represents a productive or counteracting structural element for protein aggregation. We have addressed this issue by studying the aggregation of pH-unfolded HypF-N. It has previously been shown that the two native α-helices of HypF-N retain a partial α-helical structure in the pH-unfolded state and that these regions are also involved in the formation of the cross-β structure of the aggregates. We have introduced mutations in such stretches of the sequence, with the aim of increasing the α-helical structure in the key regions of the pH-unfolded state, while minimizing the changes of other factors known to influence protein aggregation, such as hydrophobicity, β-Sheet propensity, etc. The resulting HypF-N mutants have higher contents of α-helical structure at the site(s) of mutation in their pH-unfolded states, but such an increase does not correlate with a change of aggregation rate. The results suggest that stabilisation of α-helical structure in amyloidogenic regions of the sequence of highly dynamic states does not have remarkable effects on the rate of protein aggregation from such conformational states. Comparison with other protein systems indicate that the effect of increasing α-helical propensity can vary if the stabilised helices are in non-amyloidogenic stretches of initially unstructured peptides (accelerating effect), in amyloidogenic stretches of initially unstructured peptides (no effect) or in amyloidogenic stretches of initially stable helices (decelerating effect

Online Classification of Transient EMG Patterns for the Control of the Wrist and Hand in a Transradial Prosthesis

Decoding human motor intentions by processing electrophysiological signals is a crucial, yet unsolved, challenge for the development of effective upper limb prostheses. Pattern recognition of continuous myoelectric (EMG) signals represents the state-of-art for multi-DoF prosthesis control. However, this approach relies on the unreliable assumption that repeatable muscular contractions produce repeatable patterns of steady-state EMGs. Here, we propose an approach for decoding wrist and hand movements by processing the signals associated with the onset of contraction (transient EMG). Specifically, we extend the concept of a transient EMG controller for the control of both wrist and hand, and tested it online. We assessed it with one transradial amputee and 15 non-amputees via the Target Achievement Control test. Non-amputees successfully completed 95% of the trials with a median completion time of 17 seconds, showing a significant learning trend (p < 0.001). The transradial amputee completed about the 80% of the trials with a median completion time of 26 seconds. Although the performance proved comparable with earlier studies, the long completion times suggest that the current controller is not yet clinically viable. However, taken collectively, our outcomes reinforce earlier hypothesis that the transient EMG could represent a viable alternative to steady-state pattern recognition approaches

Bridge deck flutter derivatives: efficient numerical evaluation exploiting their interdependence

Increasing the efficiency in the process to numerically compute the flutter derivatives of bridge deck sections is desirable to advance the application of CFD based aerodynamic design in industrial projects. In this article, a 2D unsteady Reynolds-averaged Navier-Stokes (URANS) approach adopting Menter׳s SST k-ω turbulence model is employed for computing the flutter derivatives and the static aerodynamic characteristics of two well known examples: a rectangular cylinder showing a completely reattached flow and the generic G1 section representative of streamlined deck sections. The analytical relationships between flutter derivatives reported in the literature are applied with the purpose of halving the number of required numerical simulations for computing the flutter derivatives. The solver of choice has been the open source code OpenFOAM. It has been found that the proposed methodology offers results which agree well with the experimental data and the accuracy of the estimated flutter derivatives is similar to the results reported in the literature where the complete set of numerical simulations has been performed for both heave and pitch degrees of freedom

Spintronic magnetic anisotropy

An attractive feature of magnetic adatoms and molecules for nanoscale applications is their superparamagnetism, the preferred alignment of their spin along an easy axis preventing undesired spin reversal. The underlying magnetic anisotropy barrier --a quadrupolar energy splitting-- is internally generated by spin-orbit interaction and can nowadays be probed by electronic transport. Here we predict that in a much broader class of quantum-dot systems with spin larger than one-half, superparamagnetism may arise without spin-orbit interaction: by attaching ferromagnets a spintronic exchange field of quadrupolar nature is generated locally. It can be observed in conductance measurements and surprisingly leads to enhanced spin filtering even in a state with zero average spin. Analogously to the spintronic dipolar exchange field, responsible for a local spin torque, the effect is susceptible to electric control and increases with tunnel coupling as well as with spin polarization.Comment: 6 pages with 4 figures + 26 pages of Supplementary Informatio

Proteome-wide observation of the phenomenon of life on the edge of solubility.

To function effectively proteins must avoid aberrant aggregation, and hence they are expected to be expressed at concentrations safely below their solubility limits. By analyzing proteome-wide mass spectrometry data of Caenorhabditis elegans, however, we show that the levels of about three-quarters of the nearly 4,000 proteins analyzed in adult animals are close to their intrinsic solubility limits, indeed exceeding them by about 10% on average. We next asked how aging and functional self-assembly influence these solubility limits. We found that despite the fact that the total quantity of proteins within the cellular environment remains approximately constant during aging, protein aggregation sharply increases between days 6 and 12 of adulthood, after the worms have reproduced, as individual proteins lose their stoichiometric balances and the cellular machinery that maintains solubility undergoes functional decline. These findings reveal that these proteins are highly prone to undergoing concentration-dependent phase separation, which on aging is rationalized in a decrease of their effective solubilities, in particular for proteins associated with translation, growth, reproduction, and the chaperone system

Proteome-wide observation of the phenomenon of life on the edge of solubility

To function effectively proteins must avoid aberrant aggregation, and hence they are expected to be expressed at concentrations safely below their solubility limits. By analyzing proteome-wide mass spectrometry data of Caenorhabditis elegans, however, we show that the levels of about three-quarters of the nearly 4, 000 proteins analyzed in adult animals are close to their intrinsic solubility limits, indeed exceeding them by about 10% on average. We next asked how aging and functional self-assembly influence these solubility limits. We found that despite the fact that the total quantity of proteins within the cellular environment remains approximately constant during aging, protein aggregation sharply increases between days 6 and 12 of adulthood, after the worms have reproduced, as individual proteins lose their stoichiometric balances and the cellular machinery that maintains solubility undergoes functional decline. These findings reveal that these proteins are highly prone to undergoing concentration-dependent phase separation, which on aging is rationalized in a decrease of their effective solubilities, in particular for proteins associated with translation, growth, reproduction, and the chaperone system