Molecular docking studies of natural compounds of naringin on enzymes involved in the urea cycle pathway in hyperammonemia

Purpose: To investigate the anti-hyperammonemic activity of naringin by molecular docking via in silico studies.Methods: Urea cycle proteins were docked to the natural compound naringin as well as a standard drug, sodium benzoate. Hydrogen bonds and binding energy were obtained using Catalytic Site Atlas and Cast P Finder Software Tool.Results: There were six urea cycle enzymes, including N-acetyl glutamate synthase, carbamoyl phosphate synthase I, ornithine transcarbamylase, argininosuccinate synthase, argininosuccinate lyase and arginase I. On evaluating protein interactions with naringin, which is dynamically  connected to the urea cycle pathway with hyperammonemia, naringin showed more hydrogen bonds and also produced higher binding energy when compared to the standard drug, sodium benzoate.Conclusion: The results of the molecular docking study show that naringin interacts with urea cycle enzymes with more hydrogen bonds and higher bonding energy than the standard drug, sodium benzoate. This supports the hypothesis that naringin can prevent experimental hyperammonemia. Keywords: Naringin, Sodium benzoate, Hyperammonemia, Urea cycle enzymes, In silico studies &nbsp

ANTIHYPERGLYCEMIC AND ANTIOXIDANT ACTIVITY OF PRUNETIN IN STREPTOZOTOCIN-INDUCED DIABETIC NEPHROTOXIC RATS

In the present study, we evaluated the protective impacts of Prunetin (PRU; 5-hydroxy-3-(4-hydroxyphenyl)-7-methoxy-4H-chromen-4-one / 5,4'-dihydroxy-7-methoxyisoflavone) altering plasma lipid peroxides and antioxidant in streptozotocin (STZ)-induced diabetic nephrotoxic (DN) rats. Wistar albino rats were given a single intraperitoneal (i.p) dosage of 45 mg/kg of STZ to cause diabetic nephropathy, which was then treated with Group-III, an effective dose of prunetin of 80 mg/kg. We examined the levels of body weight, blood glucose, insulin, lipid peroxidation products, and antioxidants in experimental rats. In STZ -induced diabetic rats, we observed an increased blood glucose level and reduced levels of insulin as compared to control rats. Whereas prunetin administration to diabetic nephrotoxic-induced rats resulted in significantly lower blood glucose level and increased the insulin levels as compared to DN rats. In diabetic nephrotoxic rats, prunetin treatment enhanced the antioxidant enzymes activity in the liver and renal tissues. Overall, the finding suggests that prunetin have significant an antioxidant and hypoglycemic activity in STZ-induced Wistar rats by scavenging free radicals thereby reducing the risk of diabetic complecations

Green Synthesised Silver Nanoparticles Using <i>Anoectochilus elatus</i> Leaf Extract: Characterisation and Evaluation of Antioxidant, Anti-Inflammatory, Antidiabetic, and Antimicrobial Activities

The present study investigates the green synthesis of silver nanoparticles was carried out using a leaf extract of Anoectochilus elatus (Ae-AgNPs). The synthesised Ae-AgNPs were characterised using different analytical techniques like UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR), and scanning electron microscopy (SEM) with energy-dispersive X-ray analysis (EDX). Additionally, in vitro activities were investigated, and they possess antioxidant, anti-inflammatory, antidiabetic, and antimicrobial properties. The UV-Vis spectra exhibited characteristic absorption peaks at approximately 480 nm. FTIR identified functional groups of the Ae-AgNPs. The crystalline structure of the Ae-AgNPs was verified via XRD analysis. SEM studies revealed that the nanoparticles exhibited a spherical morphology. The fabrication of Ae-AgNPs was established by the EDX spectrum, which exhibited prominent signals of silver atoms. The Ae-AgNPs show potent antioxidant, anti-inflammatory, and antidiabetic activity compared to standard drugs. In addition, Ae-AgNPs demonstrated the most significant zone of Inhibition. This study affirms the superior biological capability of Ae-AgNPs for target drug delivery and their potential for usage in biomedical research and therapeutics

Cytotoxic and genotoxic properties of silver nanoparticles synthesized by ethanolic extract of Salacia chinensis

International audienceIn this study, in vitro and in vivo methods were used to evaluate the cytotoxicity and genotoxicity properties of silver nanoparticles (Ag-NPs) made from a crude ethanolic extract of Salacia chinensis. The test Ag-NPs had no cytotoxicity on the fibroblast cell line at a concentration of 100 μg/mL, according to the MTT assay results. The Chinese hamster ovary (CHO) cell line treated with varied concentrations of test Ag-NPs, with a maximum concentration of 200 μg/mL, did not exhibit any appreciable genotoxic activity, either by comparing the results with positive controls of genotoxicity caused by Methyl methane sulfonate and Benzo (a) pyrene at the concentration of 20 μg/mL, the lack of genotoxicity was established. An in vivo study in Swiss albino mice using various concentrations (250, 500, and 1000 mg/kg) of test Ag-NPs, which were compared with positive controls, further confirmed this in vitro result pattern. Contrary to the genotoxicity caused by the positive control, mouse bone marrow micronucleus testing findings revealed the absence of genotoxicity. These findings imply that at the measured doses, the Ag-NPs produced from the crude ethanolic extract of Salacia chinensis do not exhibit any cytotoxicity or genotoxicity