Detecting Steps Walking at very Low Speeds Combining Outlier Detection, Transition Matrices and Autoencoders from Acceleration Patterns

In this paper, we develop and validate a new algorithm to detect steps while walking at speeds between 30 and 40 steps per minute based on the data sensed from a single tri-axial accelerometer. The algorithm concatenates three consecutive phases. First, an outlier detection is performed on the sensed data based on the Mahalanobis distance to pre-detect candidate points in the acceleration time series that may contain a ground contact segment of data while walking. Second, the acceleration segment around the pre-detected point is used to calculate the transition matrix in order to capture the time dependencies. Finally, autoencoders, trained with data segments containing ground contact transition matrices from acceleration series from labeled steps are used to reconstruct the computed transition matrices at each pre-detected point. A similarity index is used to assess if the pre-selected point contains a true step in the 30-40 steps per minute speed range. Our experimental results, based on a database from three different participants performing similar activities to the target one, are able to achieve a recall = 0.88 with precision = 0.50 improving the results when directly applying the autoencoders to acceleration patterns (recall = 0.77 with precision = 0.50)

Thermodynamic Analysis and Process System Comparison of the Exhaust Gas Recirculated, Steam Injected and Humidified Micro Gas Turbine

Stringent environmental emission regulations and continuing efforts to reduce carbon dioxide (CO2) from the energy sector, in the context of global warming, have promoted interest to improve the efficiency of power generation systems whilst reducing emissions. Further, this has led to the development of innovative gas turbine systems which either result in higher electrical efficiency or the reduction of CO2 emissions. Micro gas turbines are one of the secure, economical and environmentally viable options for power and heat generation. Here, a Turbec T100 micro gas turbine (MGT) is simulated using Aspen HYSYS® V8.4 and validated through experimental data. Due to the consistency and robustness of the steady state model developed, it is further extended to three different innovative cycles: (i) an exhaust gas recirculated (EGR) cycle, in which part of the exhaust gas is dried and re-circulated to the MGT inlet; (ii) a steam injected (STIG) cycle, and (iii) a humid air turbine (HAT) cycle. The steam and hot water are generated through the exhaust of the recuperator for the STIG and HAT cycle, respectively. Further, the steam is directly injected into the recuperator for power augmentation, while for the HAT cycle; the compressed air is saturated with water in the humid tower before entering the recuperator. This study evaluates the impact of the EGR ratio, steam to air ratio, and water to air ratio on the performance and efficiency of the system. The comparative potential for each innovative cycle is assessed by thermodynamic properties estimation of process parameters through the models developed to better understand the behavior of each cycle. The thermodynamic assessment indicates that CO2 enrichment occurs for the three innovative cycles. Further, the results indicate that the electrical efficiency increases for the STIG and HAT cycle while it decreases for the EGR cycle. In conclusion, the innovative cycles indicates the possibilities to improve the system performance and efficiency

miR-10a is aberrantly overexpressed in Nucleophosmin1 mutated acute myeloid leukaemia and its suppression induces cell death

<p>Abstract</p> <p>Background</p> <p>Acute myeloid leukaemia (AML) with nucleophosmin-1 (<it>NPM1</it>) mutation is a major subtype of AML. The <it>NPM1 </it>mutation induces a myeloproliferative disorder, but evidence indicates that other insults are necessary for the development of AML. We utilised microRNA microarrays and functional assays to determine if microRNA dysregulation could be involved in the pathogenesis of in <it>NPM1 </it>mutated (<it>NPM1^mut</it>)-AML.</p> <p>Results</p> <p>We used a stringent locked nucleic acid (LNA) based microRNA microarray platform to profile bone marrow samples of patients with normal karyotype AML. A panel of five microRNAs dichotomised AML patients according to their <it>NPM1 </it>mutational status. miR-10a, let-7b and let-7c were significantly over-expressed, while miR-130a and miR-335 were under-expressed in <it>NPM1^mut</it>-AML when compared to <it>NPM1^wildtype</it>-AML. Of these, miR-10a is the most differentially expressed in <it>NPM1^mut</it>-AML versus <it>NPM1^wildtype</it>-AML (> 10 fold higher as confirmed by qRT-PCR). To investigate the functions of miR-10a, the OCI-AML3 cell line was utilised, which is the only commercially available cell line bearing <it>NPM1^mut</it>. OCI-AML3 cells were firstly demonstrated to have a similarly high miR-10a expression to primary <it>NPM1^mut</it>-AML patient samples. Inhibition of miR-10a expression by miRCURY LNA Inhibitors (Exiqon) in these cells resulted in increased cell death as assessed by MTS, cell cycle and Annexin-V assays and reduced clonogenic capacity, indicative of an involvement in leukaemic cell survival. <it>In silico </it>filtering of bioinformatically predicted targets of miR-10a identified a number of potential mRNA targets with annotated functions in haematopoiesis, cell growth and apoptosis. Lucferase reporter assays confirmed a number of these putative tumorogenic genes that are miR-10a suppressible including <it>KLF4 </it>and <it>RB1CC1</it>. This provides a potential mechanism for the pathogenic role of miR-10a in <it>NPM1^mut</it>-AML.</p> <p>Conclusions</p> <p>This study provides, for the first time, <it>in vitro </it>evidence of a pro-survival role of miR-10a in <it>NPM1^mut</it>-AML, that it may contribute to the pathogenesis of <it>NPM1^mut</it>-AML and identifies putative tumorogenic targets.</p

Quality improvement of medical records in a teaching hospital

Introduction. The aim of this study was to evaluate the quality of the MR compilation in some Operative Units of the ?Azienda Ospedaliera Universitaria - II Università di Napoli? (AOU- SUN) - Italy, before and after an intervention of quality improve- ment, underlining the potential differences in the behaviour of dif- ferent specialists (physicians vs. surgeons). Methods. Two random samples of 660 MRs were reviewed. A four-step program was developed: 1) first assessment of the MR; 2) implementation of the MR quality, sending a letter with the purpose of the study, the results obtained in the first step from that ward, the guidelines to correctly fill out the MR; 3) follow-up step four months later; 4) comparison of the data before and after the distribution of the guidelines using indicators of completeness of all sections of MR, clarity of handwriting and presence and clar- ity of signature. Results. The main concerns were related to the signature of the duty physician (present in 2.0% and legible in only 15.4%), the presence of the letter of discharge (18.0%) and the clarity of the days of hospital stay (32.0%). After the intervention the improvement of the quality of compilation was modest and regarded mainly medical rather than surgical wards. Discussion and conclusions. The improvement was not satisfying since from a medical and a legal point of view the indicators should reach 100% of clarity and completeness. A further study is being carried out to improve the involvement of health care professional, so that such requirements will be perceived as a common goal, not as mere bureaucratic initiatives

Effects of pilates exercise programs in people with chronic low back pain: a systematic review

The Pilates method has recently become a fast-growing popular way of exercise recommended for healthy individuals and those engaged in rehabilitation. Several published studies have examined the effects of Pilates method in people with chronic low back pain (LBP).The objective of this study is to describe and provide an extensive overview of the scientific literature comparing the effectiveness of the Pilates method on pain and disability in patients with chronic nonspecific LBP. The study is based on the data from the following sources: MEDLINE-NLM, MEDLINE-EBSCO, Scopus Elsevier, Cochrane, DOAJ, SciELO, and PLOSONE.Original articles and systematic reviews of adults with chronic nonspecific LBP that evaluated pain and/or disability were included in this study; studies in which the primary treatment was based on Pilates method exercises compared with no treatment, minimal intervention, other types of intervention, or other types of exercises.The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) were adopted. The literature search included 7 electronic databases and the reference list of relevant systematic reviews and original articles to July 2014. Two independent investigators conducted the literature search and performed the synthesis as follows: Study Design; Sample (n); Disability measure; Intervention; and Main results.The searches identified a total of 128 articles. From these, 29 were considered eligible and were included in the analysis. The items were stratified as follows: Pilates method versus other kind of exercises (n = 6 trials) and Pilates method versus no treatment group or minimal intervention for short-term pain (n = 9 trials); the therapeutic effect of the Pilates method in randomized cohorts (n = 5); and analysis of reviews (n = 9).We found that there is a dearth of studies that clearly demonstrates the efficacy of a specific Pilates exercise program over another in the treatment of chronic pain. However, the consensus in the field suggests that Pilates method is more effective than minimal physical exercise intervention in reducing pain. These conclusions need to be supported by other proper investigations

Diagnosis and treatment of Chiari malformation type 1 in children: the International Consensus Document

Malformació de Chiari 1; Nens; SiringomieliaMalformación de Chiari 1; Niños; SiringomieliaChiari 1 malformation; Children; SyringomyeliaBackground Chiari malformation type 1 (CM1) is a rare condition where agreed classification and treatment are still missing. The goal of this study is to achieve a consensus on the diagnosis and treatment of CM1 in children. Methods A multidisciplinary panel formulated 57 provisional statements based on a review of the literature. Thirty-four international experts (IE) participated in a Delphi study by independently rating each statement on a 4-point Likert scale (“strongly disagree,” “disagree,” “agree,” “strongly agree”). Statements that were endorsed (“agree” or “strongly agree”) by < 75% of raters were re-formulated, or new statements were added, and another Delphi round followed (up to a maximum of three). Results Thirty-five IE were contacted and 34 agreed to participate. A consensus was reached on 30/57 statements (52.6%) after round 1. Three statements were added, and one removed. After round 2, agreement was reached on 56/59 statements (94.9%). Finally, after round 3, which took place during the 2019 Chiari Consensus Conference (Milan, Italy), agreement was reached on 58/59 statements (98.3%) about four main sections (Definition and Classification, Planning, Surgery, Isolated Syringomyelia). Only one statement did not gain a consensus, which is the “definition of radiological failure 24 month post-surgery.” Conclusions The consensus document consists of 58 statements (24 on diagnosis, 34 on treatment), serving clinicians and researchers following children with CM1. There is a clear need for establishing an international network and registry and to promote collaborative studies to increase the evidence base and optimize the long-term care of this patient population.Open access funding provided by Università Cattolica del Sacro Cuore within the CRUI-CARE Agreement

Universal Quantum Computation with the Exchange Interaction

Experimental implementations of quantum computer architectures are now being investigated in many different physical settings. The full set of requirements that must be met to make quantum computing a reality in the laboratory [1] is daunting, involving capabilities well beyond the present state of the art. In this report we develop a significant simplification of these requirements that can be applied in many recent solid-state approaches, using quantum dots [2], and using donor-atom nuclear spins [3] or electron spins [4]. In these approaches, the basic two-qubit quantum gate is generated by a tunable Heisenberg interaction (the Hamiltonian is $H_{ij}=J(t){\vec S}_i\cdot{\vec S}_j$ between spins $i$ and $j$), while the one-qubit gates require the control of a local Zeeman field. Compared to the Heisenberg operation, the one-qubit operations are significantly slower and require substantially greater materials and device complexity, which may also contribute to increasing the decoherence rate. Here we introduce an explicit scheme in which the Heisenberg interaction alone suffices to exactly implement any quantum computer circuit, at a price of a factor of three in additional qubits and about a factor of ten in additional two-qubit operations. Even at this cost, the ability to eliminate the complexity of one-qubit operations should accelerate progress towards these solid-state implementations of quantum computation.Comment: revtex, 2 figures, this version appeared in Natur

Immunophenotype of Atypical Polypoid Adenomyoma of the Uterus: Diagnostic Value and Insight on Pathogenesis

Atypical polypoid adenomyoma (APA) is a rare uterine lesion constituted by atypical endometrioid glands, squamous morules, and myofibromatous stroma. We aimed to assess the immunophenotype of the 3 components of APA, with regard to its pathogenesis and its differential diagnosis. A systematic review was performed by searching electronic databases from their inception to January 2019 for immunohistochemical studies of APA. Thirteen studies with 145 APA cases were included. APA glands appeared analogous to atypical endometrial hyperplasia (endometrioid cytokeratins pattern, Ki67≤50%, common PTEN loss, and occasional mismatch repair deficiency); the prominent expression of hormone receptors and nuclear β-catenin suggest that APA may be a precursor of "copy number-low," CTNNB1-mutant endometrial cancers. Morules appeared as a peculiar type of hyperdifferentiation (low KI67, nuclear β-catenin+, CD10+, CDX2+, SATB2+, p63-, and p40-), analogous to morular metaplasia in other lesions and distinguishable immunohistochemically from both conventional squamous metaplasia and solid cancer growth. Stroma immunphenotype (low Ki67, α-smooth-muscle-actin+, h-caldesmon-, CD10-, or weak and patchy) suggested a derivation from a metaplasia of normal endometrial stroma. It was similar to that of nonatypical adenomyoma, and different from adenosarcoma (Ki67 increase and CD10+ in periglandular stroma) and myoinvasive endometrioid carcinoma (h-caldesmon+ in myometrium and periglandular fringe-like CD10 pattern)

Immune-mediated mechanisms influencing the efficacy of anticancer therapies

Conventional anticancer therapies, such as chemotherapy, radiotherapy, and targeted therapy, are designed to kill cancer cells. However, the efficacy of anticancer therapies is not only determined by their direct effects on cancer cells but also by off-target effects within the host immune system. Cytotoxic treatment regimens elicit several changes in immune-related parameters including the composition, phenotype, and function of immune cells. Here we discuss the impact of innate and adaptive immune cells on the success of anticancer therapy. In this context we examine the opportunities to exploit host immune responses to boost tumor clearing, and highlight the challenges facing the treatment of advanced metastatic disease