The impact of multidisciplinary team management on outcome of hepatic resection in liver-limited colorectal metastases

Hepatic resection is the gold standard treatment for patients affected by liver-limited colorectal metastases. Reports addressing the impact of multidisciplinary team (MDT) evaluation on survival are controversial. The aim of this study was to evaluate the benefit of MDT management in these patients in our Institution experience. The objective of the analysis was to compare survivals of patients managed within our MDT (MDT cohort) to those of patients referred to surgery from other hospitals without MDT discussion (non-MDT cohort). Of the 523 patients, 229 were included in the MDT cohort and 294 in the non-MDT cohort. No difference between the two groups was found in terms of median overall survival (52.5 vs 53.6 months; HR 1.13; 95% CI, 0.88–1.45; p = 0.344). In the MDT cohort there was a higher number of metastases (4.5 vs 2.7; p < 0.0001). The median duration of chemotherapy was lower in MDT patients (8 vs 10 cycles; p < 0.001). Post-operative morbidity was lower in the MDT cohort (6.2 vs 21.5%; p < 0.001). One hundred and ninety-seven patients in each group were matched by propensity score and no significant difference was observed between the two groups in terms of OS and DFS. Our study does not demonstrate a survival benefit from MDT management, but it allows surgery to patients with a more advanced disease. MDT assessment reduces the median duration of chemotherapy and post-operative morbidities

Cyclin D1 promotes neurogenesis in the developing spinal cord in a cell cycle-independent manner

Neural stem and progenitor cells undergo an important transition from proliferation to differentiation in the G1 phase of the cell cycle. The mechanisms coordinating this transition are incompletely understood. Cyclin D proteins promote proliferation in G1 and typically are down-regulated before differentiation. Here we show that motoneuron progenitors in the embryonic spinal cord persistently express Cyclin D1 during the initial phase of differentiation, while down-regulating Cyclin D2. Loss-of-function and gain-offunction experiments indicate that Cyclin D1 (but not D2) promotes neurogenesis in vivo, a role that can be dissociated from its cell cycle function. Moreover, reexpression of Cyclin D1 can restore neurogenic capacity to D2-expressing glial-restricted progenitors. The neurogenic function of Cyclin D1 appears to be mediated, directly or indirectly, by Hes6, a proneurogenic basic helic-loop-helix transcription factor. These data identify a cell cycle-independent function for Cyclin D1 in promoting neuronal differentiation, along with a potential genetic pathway through which this function is exerted

Co-ordination of cell cycle and differentiation in the developing nervous system

During embryonic development, cells must divide to produce appropriate numbers, but later must exit the cell cycle to allow differentiation. How these processes of proliferation and differentiation are co-ordinated during embryonic development has been poorly understood until recently. However, a number of studies have now given an insight into how the cell cycle machinery, including cyclins, CDKs (cyclin-dependent kinases), CDK inhibitors and other cell cycle regulators directly influence mechanisms that control cell fate and differentiation. Conversely, examples are emerging of transcriptional regulators that are better known for their role in driving the differentiated phenotype, which also play complementary roles in controlling cell cycle progression. The present review will summarise our current understanding of the mechanisms co-ordinating the cell cycle and differentiation in the developing nervous system, where these links have been, perhaps, most extensively studied

Microalgae Schizochytrium sp. in diets for Piau juvenilles

Todos os textos, informa??es e resultados apresentados s?o de inteira responsabilidade dos autores.O DHA ? um ?cido importante da s?rie ?mega 3, fundamental para a forma??o do tecido nervoso e visual em humanos, e confere tamb?m uma boa forma??o da massa encef?lica. O presente trabalho teve o objetivo de avaliar dietas contendo a inclus?o da alga Schizochytrium sp. no desempenho produtivo de juvenis de piau.O experimento foi conduzido no Laborat?rio de Aquicultura e Ecologia Aqu?tica do Departamento de Zootecnia da Universidade Federal dos Vales do Jequitinhonha e Mucuri, em Diamantina MG. Foram utilizados 300 juvenis de Piau (Leporinus friderici) com peso e comprimento total m?dio de 11,8 e 9,68 g respectivamente . Os peixes passaram por sete dias de adapta??o e foram alimentados com a ra??o do tratamento controle. . O experimento foi realizado em um delineamento inteiramente casualizado, com cinco tratamentos (0, 10, 20, 30, e 40 g de Schizochytrium sp/kg-1)e quatro repeti??es. Sete juvenis foram estocados por aqu?rio, totalizando 20 parcelas experimentais. Avaliou-se aos 60 dias de experimento par?metros de desempenho produtivo. Foram mensurados: comprimento padr?o (cm), comprimento total (cm), ganho de peso (g), consumo de ra??o (g), convers?o alimentar (g/g)?, biomassa final (g), e sobreviv?ncia (%). As vari?veis de desempenho apresentaram melhores resultados de acordo com o aumento do n?vel de inclus?o da alga Schizochytrium sp em juvenis de piau. Conlcui-se com este estudo que juvenis de piau alimentados com 40g de Schizochytrium sp.kg-1 apresentam melhor desempenho produtivo e sobreviv?ncia.DHA is an important acid of the omega-3 series, essential for the formation of the nervous and visual tissue in humans, and also gives a good formation of brain matter. This study aimed to evaluate diets with the inclusion of algae Schizochytrium sp. In productive performance of piau juveniles.. Experiment was conducted at the Aquaculture Laboratory of Aquatic Ecology of the Department of Animal Science, of the Federal University of the Jequitinhonha and Mucuri Valleys, in Diamantina MG. . Three hundred Piau (Leporinus friderici) juvenile, with body weight and length means of 11.8, and 9.68 g respectively. Fish have undergone seven days of adaptation and were fed with the control ration treatment. The experiment was conducted in a completely randomized design, with five treatments (0, 10, 20, 30, and 40 g of Schizochytrium sp. Kg-1) and four replications. Seven juveniles were stocked per aquarium, totaling 20 experimental plots. It was evaluated at 60 days of the experiment For the productive performance were measured: standard length (cm), total length (cm), weight gain (g), feed intake (g), feed conversion (g / g) ? , final biomass (g) EC (%) and survival (%). The performance variables showed greater results in accordance with the increase in the level of inclusionof Schizochytrium sp in piau juveniles diets. It follows that piau juveniles fed with 40g Schizochytrium sp.kg-1 have greater growth performance and survival

Caprin Controls Follicle Stem Cell Fate in the Drosophila Ovary

Adult stem cells must balance self-renewal and differentiation for tissue homeostasis. The Drosophila ovary has provided a wealth of information about the extrinsic niche signals and intrinsic molecular processes required to ensure appropriate germline stem cell renewal and differentiation. The factors controlling behavior of the more recently identified follicle stem cells of the ovary are less well-understood but equally important for fertility. Here we report that translational regulators play a critical role in controlling these cells. Specifically, the translational regulator Caprin (Capr) is required in the follicle stem cell lineage to ensure maintenance of this stem cell population and proper encapsulation of developing germ cells by follicle stem cell progeny. In addition, reduction of one copy of the gene fmr1, encoding the translational regulator Fragile X Mental Retardation Protein, exacerbates the Capr encapsulation phenotype, suggesting Capr and fmr1 are regulating a common process. Caprin was previously characterized in vertebrates as Cytoplasmic Activation/Proliferation-Associated Protein. Significantly, we find that loss of Caprin alters the dynamics of the cell cycle, and we present evidence that misregulation of CycB contributes to the disruption in behavior of follicle stem cell progeny. Our findings support the idea that translational regulators may provide a conserved mechanism for oversight of developmentally critical cell cycles such as those in stem cell populations

Re-cycling paradigms: cell cycle regulation in adult hippocampal neurogenesis and implications for depression

Since adult neurogenesis became a widely accepted phenomenon, much effort has been put in trying to understand the mechanisms involved in its regulation. In addition, the pathophysiology of several neuropsychiatric disorders, such as depression, has been associated with imbalances in adult hippocampal neurogenesis. These imbalances may ultimately reflect alterations at the cell cycle level, as a common mechanism through which intrinsic and extrinsic stimuli interact with the neurogenic niche properties. Thus, the comprehension of these regulatory mechanisms has become of major importance to disclose novel therapeutic targets. In this review, we first present a comprehensive view on the cell cycle components and mechanisms that were identified in the context of the homeostatic adult hippocampal neurogenic niche. Then, we focus on recent work regarding the cell cycle changes and signaling pathways that are responsible for the neurogenesis imbalances observed in neuropathological conditions, with a particular emphasis on depression

Duffy blood group gene polymorphisms among malaria vivax patients in four areas of the Brazilian Amazon region

<p>Abstract</p> <p>Background</p> <p>Duffy blood group polymorphisms are important in areas where <it>Plasmodium vivax </it>predominates, because this molecule acts as a receptor for this protozoan. In the present study, Duffy blood group genotyping in <it>P. vivax </it>malaria patients from four different Brazilian endemic areas is reported, exploring significant associations between blood group variants and susceptibility or resistance to malaria.</p> <p>Methods</p> <p>The <it>P. vivax </it>identification was determined by non-genotypic and genotypic screening tests. The Duffy blood group was genotyped by PCR/RFLP in 330 blood donors and 312 malaria patients from four Brazilian Amazon areas. In order to assess the variables significance and to obtain independence among the proportions, the Fisher's exact test was used.</p> <p>Results</p> <p>The data show a high frequency of the <it>FYA/FYB </it>genotype, followed by <it>FYB/FYB, FYA/FYA</it>, <it>FYA/FYB-33 </it>and <it>FYB/FYB-33</it>. Low frequencies were detected for the <it>FYA/FY</it>^<it>X</it>, <it>FYB/FY</it>^<it>X</it>, <it>FYX/FY</it>^{<it>X </it>}and <it>FYB-33/FYB-33 </it>genotypes. Negative Duffy genotype (<it>FYB-33/FYB-33</it>) was found in both groups: individuals infected and non-infected (blood donors). No individual carried the <it>FY</it>^<it>X</it><it>/FYB-33 </it>genotype. Some of the Duffy genotypes frequencies showed significant differences between donors and malaria patients.</p> <p>Conclusion</p> <p>The obtained data suggest that individuals with the <it>FYA/FYB </it>genotype have higher susceptibility to malaria. The presence of the <it>FYB-33 </it>allele may be a selective advantage in the population, reducing the rate of infection by <it>P. vivax </it>in this region. Additional efforts may contribute to better elucidate the physiopathologic differences in this parasite/host relationship in regions endemic for <it>P. vivax </it>malaria, in particular the Brazilian Amazon region.</p