Global surface-ocean pCO2 and sea–air CO2 flux variability from an observation-driven ocean mixed-layer scheme

A temporally and spatially resolved estimate of the global surface-ocean CO₂ partial pressure field and the sea–air CO₂ flux is presented, obtained by fitting a simple data-driven diagnostic model of ocean mixed-layer biogeochemistry to surface-ocean CO₂ partial pressure data from the SOCAT v1.5 database. Results include seasonal, interannual, and short-term (daily) variations. In most regions, estimated seasonality is well constrained from the data, and compares well to the widely used monthly climatology by Takahashi et al. (2009). Comparison to independent data tentatively supports the slightly higher seasonal variations in our estimates in some areas. We also fitted the diagnostic model to atmospheric CO₂ data. The results of this are less robust, but in those areas where atmospheric signals are not strongly influenced by land flux variability, their seasonality is nevertheless consistent with the results based on surface-ocean data. From a comparison with an independent seasonal climatology of surface-ocean nutrient concentration, the diagnostic model is shown to capture relevant surface-ocean biogeochemical processes reasonably well. Estimated interannual variations will be presented and discussed in a companion paper

Female mate choice and male red coloration in a natural three-spined stickleback (Gasterosteus aculeatus) population

Under laboratory conditions, female three-spined sticklebacks (Gasterosteus aculeatus L.) show a mating preference for intensely red-colored males. We verified this female choice in the field by observing a freshwater stickleback population in its natural habitat. During the egg collection phase, individual courting males were localized with the aid of a dummy of a ripe female, caught and photographed under standardized conditions, and released. After males had stopped collecting eggs, we counted the number of eggs in the nests. The more intense a male's red breeding coloration, the more eggs he received. Simultaneous female choice experiments in the laboratory suggested that ripe females of this population preferred redder males. Breeding activities of the males in the field were clustered and seem to be synchronized within clusters. At one of the breeding sites, more intense red males were in better physical condition, but this was not the case at another site. Although several synchronized breeding cycles were observed, the majority of males seem to complete only one breeding cycl

Female preferences for single and combined traits in computer animated stickleback males

In many animal species, males have more than one secondary sexual character. Apart from theoretical considerations about the evolution of multiple traits, there are almost no empirical studies on female mate choice decisions based on combinations of traits as opposed to decisions based on single traits. Because three-spined sticklebacks are exceptionally well suited to be tested with computer animation technique, which itself fills the gap of adequate test paradigms for multiple traits, we tested female sticklebacks for their preferences for both single and combined male traits. We used virtual stickleback males that differed either in red throat coloration, courtship intensity, body size, or in combinations of these traits. The virtual male with increased redness was found to be preferred by females, whereas the male courting more intensely was not. The tests for combinations of traits revealed the more pronounced female preferences, the more traits were available to the females to judge male qualit

Hamilton-Jacobi formalism for Linearized Gravity

In this work we study the theory of linearized gravity via the Hamilton-Jacobi formalism. We make a brief review of this theory and its Lagrangian description, as well as a review of the Hamilton-Jacobi approach for singular systems. Then we apply this formalism to analyze the constraint structure of the linearized gravity in instant and front-form dynamics.Comment: To be published in Classical and Quantum Gravit

Tracer Measurements in Growing Sea Ice Support Convective Gravity Drainage Parameterizations

Gravity drainage is the dominant process redistributing solutes in growing sea ice. Modeling gravity drainage is therefore necessary to predict physical and biogeochemical variables in sea ice. We evaluate seven gravity drainage parameterizations, spanning the range of approaches in the literature, using tracer measurements in a sea ice growth experiment. Artificial sea ice is grown to around 17 cm thickness in a new experimental facility, the Roland von Glasow air‐sea‐ice chamber. We use NaCl (present in the water initially) and rhodamine (injected into the water after 10 cm of sea ice growth) as independent tracers of brine dynamics. We measure vertical profiles of bulk salinity in situ, as well as bulk salinity and rhodamine in discrete samples taken at the end of the experiment. Convective parameterizations that diagnose gravity drainage using Rayleigh numbers outperform a simpler convective parameterization and diffusive parameterizations when compared to observations. This study is the first to numerically model solutes decoupled from salinity using convective gravity drainage parameterizations. Our results show that (1) convective, Rayleigh number‐based parameterizations are our most accurate and precise tool for predicting sea ice bulk salinity; and (2) these parameterizations can be generalized to brine dynamics parameterizations, and hence can predict the dynamics of any solute in growing sea ic

Variability and nature of the binary in the Red Rectangle Nebula

We present new observations of the central binary inside the Red Rectangle nebula. The detection of zinc in the optical spectrum confirms that the peculiar photospheric abundances are due to accretion of circumstellar gas. Grey brightness variations with the orbital period are observed. They are interpreted as being due to the variation of the scattering angle with orbital phase. The small orbital separation of the system is not compatible with previous normal evolution of the primary on the AGB. We point out the similarity of the orbital history of this and other similar systems with those of some close Barium stars and suggest that the nonzero eccentricity of the orbit is the result of tidal interaction with the circumbinary disk.Comment: 4 pages, 3 figures, A&A Letters accepte

The canonical structure of Podolsky's generalized electrodynamics on the Null-Plane

In this work we will develop the canonical structure of Podolsky's generalized electrodynamics on the null-plane. This theory has second-order derivatives in the Lagrangian function and requires a closer study for the definition of the momenta and canonical Hamiltonian of the system. On the null-plane the field equations also demand a different analysis of the initial-boundary value problem and proper conditions must be chosen on the null-planes. We will show that the constraint structure, based on Dirac formalism, presents a set of second-class constraints, which are exclusive of the analysis on the null-plane, and an expected set of first-class constraints that are generators of a U(1) group of gauge transformations. An inspection on the field equations will lead us to the generalized radiation gauge on the null-plane, and Dirac Brackets will be introduced considering the problem of uniqueness of these brackets under the chosen initial-boundary condition of the theory

Dominant negative phenotype of Bacillus thuringiensis Cry1Ab, Cry11Aa and Cry4Ba mutants suggest hetero-oligomer formation among different Cry toxins.

Background - Bacillus thuringiensis Cry toxins are used worldwide in the control of different insect pests important in agriculture or in human health. The Cry proteins are pore-forming toxins that affect the midgut cell of target insects. It was shown that non-toxic Cry1Ab helix a-4 mutants had a dominant negative (DN) phenotype inhibiting the toxicity of wildtype Cry1Ab when used in equimolar or sub-stoichiometric ratios (1:1, 0.5:1, mutant:wt) indicating that oligomer formation is a key step in toxicity of Cry toxins. Methodology/Principal Findings - The DN Cry1Ab-D136N/T143D mutant that is able to block toxicity of Cry1Ab toxin, was used to analyze its capacity to block the activity against Manduca sexta larvae of other Cry1 toxins, such as Cry1Aa, Cry1Ac, Cry1Ca, Cry1Da, Cry1Ea and Cry1Fa. Cry1Ab-DN mutant inhibited toxicity of Cry1Aa, Cry1Ac and Cry1Fa. In addition, we isolated mutants in helix a-4 of Cry4Ba and Cry11Aa, and demonstrate that Cry4Ba-E159K and Cry11Aa-V142D are inactive and completely block the toxicity against Aedes aegypti of both wildtype toxins, when used at sub-stoichiometric ratios, confirming a DN phenotype. As controls we analyzed Cry1Ab-R99A or Cry11Aa-E97A mutants that are located in helix a-3 and are affected in toxin oligomerization. These mutants do not show a DN phenotype but were able to block toxicity when used in 10:1 or 100:1 ratios (mutant:wt) probably by competition of binding with toxin receptors. Conclusions/Significance - We show that DN phenotype can be observed among different Cry toxins suggesting that may interact in vivo forming hetero-oligomers. The DN phenotype cannot be observed in mutants affected in oligomerization, suggesting that this step is important to inhibit toxicity of other toxin