'Bunchy top symptom' of papaya in Cuba: new insights

Papaya is an important export crop in Cuba. A disease named Bunchy top symptom (BTS) was first observed in 2003 from Villa Clara province, but is now widespread throughout the country. A nested PCR assay with 16S rDNA phytoplasma primers P1/P7 and R16F2n/R2 was used to index more than 2200 papaya plants, weeds and insect samples collected between November 2005 and June 2006. RFLP patterns for all amplicons were identical with HaeIII, RsaI and AluI enzymes. No rickettsia-like bacteria were found in any of the samples. Phytoplasma rDNA was amplified from 1449 (89.7%) papaya plants with BTS symptoms. Phytoplasma rDNA was also detected in 331 apparently healthy papayas, and other plant species: Anoda acerifolia (57), Euphorbia heterophylla (73), Malvastrum coromandelianum (41) and Rynchosia minima (37) and 60/75 batches of Empoasca papayae. Sequences from the phytoplasma in papaya (DQ868531), A. acerifolia (DQ286950); E. heterophylla (DQ286951); M. coromandelianum (DQ286952); R. minima (DQ868533) and the leafhopper E. papayae (DQ868532) all showed 99% similarity to the phytoplasma associated with polygala phyllody, PPhy, (AY787140) belonging to the 16SrII group, ‘Candidatus Phytoplasma aurantifolia’. The discovery of the BTS phytoplasma in weed species and a putative vector Empoasca papayae suggests, that these plants and the leafhopper may have role in the spread of this disease

Confusing the extragalactic neutrino flux limit with a neutrino propagation limit

We study the possible suppression of the extragalactic neutrino flux due to a nonstandard interaction during its propagation. In particular, we study neutrino interaction with an ultra-light scalar field dark matter. It is shown that the extragalactic neutrino flux may be suppressed by such an interaction, leading to a new mechanism to reduce the ultra-high energy neutrino flux. We study both the cases of non-self-conjugate as well as self-conjugate dark matter. In the first case, the suppression is independent of the neutrino and dark matter masses. We conclude that care must be taken when explaining limits on the neutrino flux through source acceleration mechanisms only, since there could be other mechanisms for the reduction of the neutrino flux.Comment: 15 pages, 4 figures. Important changes implemented. Abstract modified. Conclusions remain. To be published in JCA

Single-shot d-scan technique for ultrashort laser pulse characterization using transverse second-harmonic generation in random nonlinear crystals

We demonstrate a novel dispersion-scan (d-scan) scheme for single-shot temporal characterization of ultrashort laser pulses. The novelty of this method relies on the use of a highly dispersive crystal featuring antiparallel nonlinear domains with a random distribution and size. This crystal, capable of generating a transverse second-harmonic signal, acts simultaneously as the dispersive element and the nonlinear medium of the d-scan device. The resulting in-line architecture makes the technique very simple and robust, allowing the acquisition of single-shot d-scan traces in real time. The retrieved pulses are in very good agreement with independent frequency-resolved optical grating measurements. We also apply the new single-shot d-scan to a terawatt-class laser equipped with a programmable pulse shaper, obtaining an excellent agreement between the applied and the d-scan retrieved dispersions

Interfacing Neurons with Nanostructured Electrodes Modulates Synaptic Circuit Features

Understanding neural physiopathology requires advances in nanotechnology-based interfaces, engineered to monitor the functional state of mammalian nervous cells. Such interfaces typically contain nanometer-size features for stimulation and recording as in cell-non-invasive extracellular microelectrode arrays. In such devices, it turns crucial to understand specific interactions of neural cells with physicochemical features of electrodes, which could be designed to optimize performance. Herein, versatile flexible nanostructured electrodes covered by arrays of metallic nanowires are fabricated and used to investigate the role of chemical composition and nanotopography on rat brain cells in vitro. By using Au and Ni as exemplary materials, nanostructure and chemical composition are demonstrated to play major roles in the interaction of neural cells with electrodes. Nanostructured devices are interfaced to rat embryonic cortical cells and postnatal hippocampal neurons forming synaptic circuits. It is shown that Au-based electrodes behave similarly to controls. Contrarily, Ni-based nanostructured electrodes increase cell survival, boost neuronal differentiation, and reduce glial cells with respect to flat counterparts. Nonetheless, Au-based electrodes perform superiorly compared to Ni-based ones. Under electrical stimulation, Au-based nanostructured substrates evoke intracellular calcium dynamics compatible with neural networks activation. These studies highlight the opportunity for these electrodes to excite a silent neural network by direct neuronal membranes depolarization

Bounds on the tau and muon neutrino vector and axial vector charge radius

A Majorana neutrino is characterized by just one flavor diagonal electromagnetic form factor: the anapole moment, that in the static limit corresponds to the axial vector charge radius . Experimental information on this quantity is scarce, especially in the case of the tau neutrino. We present a comprehensive analysis of the available data on the single photon production process $e^+ e^- -> \nu \bar\nu \gamma$ off Z-resonance, and we discuss the constraints that these measurements can set on for the tau neutrino. We also derive limits for the Dirac case, when the presence of a vector charge radius is allowed. Finally, we comment on additional experimental data on $\nu_\mu$ scattering from the NuTeV, E734, CCFR and CHARM-II collaborations, and estimate the limits implied for and for the muon neutrino.Comment: 20 pages, 2 eps figures. CCFR data included in the analysis. Conclusion unchange

Increased Brain Fatty Acid Uptake in Metabolic Syndrome

OBJECTIVE: To test whether brain fatty acid uptake is enhanced in obese subjects with metabolic syndrome (MS) and whether weight reduction modifies it. RESEARCH DESIGN AND METHODS: We measured brain fatty acid uptake in a group of 23 patients with MS and 7 age-matched healthy control subjects during fasting conditions using positron emission tomography (PET) with [(11)C]-palmitate and [(18)F]fluoro-6-thia-heptadecanoic acid ([(18)F]-FTHA). Sixteen MS subjects were restudied after 6 weeks of very low calorie diet intervention. RESULTS: At baseline, brain global fatty acid uptake derived from [(18)F]-FTHA was 50% higher in patients with MS compared with control subjects. The mean percentage increment was 130% in the white matter, 47% in the gray matter, and uniform across brain regions. In the MS group, the nonoxidized fraction measured using [(11)C]-palmitate was 86% higher. Brain fatty acid uptake measured with [(18)F]-FTHA-PET was associated with age, fasting serum insulin, and homeostasis model assessment (HOMA) index. Both total and nonoxidized fractions of fatty acid uptake were associated with BMI. Rapid weight reduction decreased brain fatty acid uptake by 17%. CONCLUSIONS: To our knowledge, this is the first study on humans to observe enhanced brain fatty acid uptake in patients with MS. Both fatty acid uptake and accumulation appear to be increased in MS patients and reversed by weight reduction

Contrasting changes in the abundance and diversity of North American bird assemblages from 1971 to 2010

This article is based upon work from COST Action ES1101 "Harmonising Global Biodiversity Modelling" (Harmbio), supported by COST (European Cooperation in Science and Technology).Although it is generally recognized that global biodiversity is declining, few studies have examined long-term changes in multiple biodiversity dimensions simultaneously. In this study we quantified and compared temporal changes in the abundance, taxonomic diversity, functional diversity and phylogenetic diversity of bird assemblages, using roadside monitoring data of the North American Breeding Bird Survey from 1971 to 2010. We calculated 12 abundance and diversity metrics based on five year average abundances of 519 species for each of 768 monitoring routes. We did this for all bird species together as well as for four sub-groups based on breeding habitat affinity (grassland, woodland, wetland and shrubland breeders). The majority of the biodiversity metrics increased or remained constant over the study period, whereas the overall abundance of birds showed a pronounced decrease, primarily driven by declines of the most abundant species. These results highlight how stable or even increasing metrics of taxonomic, functional or phylogenetic diversity may occur in parallel with substantial losses of individuals. We further found that patterns of change differed among the species sub-groups, with both abundance and diversity increasing for woodland birds and decreasing for grassland breeders. The contrasting changes between abundance and diversity and among the breeding habitat groups underscore the relevance of a multi-faceted approach to measuring biodiversity change. Our findings further stress the importance of monitoring the overall abundance of individuals in addition to metrics of taxonomic, functional or phylogenetic diversity, thus confirming the importance of population abundance as an essential biodiversity variable.Publisher PDFPeer reviewe

Author correction : a global database for metacommunity ecology, integrating species, traits, environment and space

Correction to: Scientific Data https://doi.org/10.1038/s41597-019-0344-7, published online 08 January 202

Measuring the ratio of the gas and dust emission radii of protoplanetary disks in the Lupus star-forming region

We perform a comprehensive demographic study of the CO extent relative to dust of the disk population in the Lupus clouds, in order to find indications of dust evolution and possible correlations with other properties. We increase up to 42 the number of disks of the region with measured CO and dust sizes ($R_{\mathrm{CO}}$, $R_{\mathrm{dust}}$) from observations with the Atacama Large Millimeter/submillimeter Array (ALMA). The sizes are obtained from modeling the ${^{12}}$CO $J = 2-1$ line emission and continuum emission at $\sim 0.89$ mm with an empirical function (Nuker profile or Gaussian function). The CO emission is more extended than the dust continuum, with a $R_{68\%}^{\mathrm{CO}}$/$R_{68\%}^{\mathrm{dust}}$ median value of 2.5, for the entire population and for a sub-sample with high completeness. 6 disks, around $15\%$ of the Lupus disk population have a size ratio above 4. Based on thermo-chemical modeling, this value can only be explained if the disk has undergone grain growth and radial drift. These disks do not have unusual properties in terms of stellar mass ($M_{\star}$), disk mass ($M_{\mathrm{disk}}$), CO and dust sizes ($R_{\mathrm{CO}}$, $R_{\mathrm{dust}}$), and mass accretion. We search for correlations between the size ratio and $M_{\star}$, $M_{\mathrm{disk}}$, $R_{\mathrm{CO}}$ and $R_{\mathrm{dust}}$: only a weak monotonic anti-correlation with the $R_{\mathrm{dust}}$ is found. The lack of strong correlations is remarkable and suggests that the bulk of the population may be in a similar evolutionary stage, independent of the stellar and disk properties. These results should be further investigated, since the optical depth difference between CO and dust continuum may play a role in the inferred size ratios. Lastly, the CO emission for the majority of the disks is consistent with optically thick emission and an average CO temperature of around 30 K.Comment: Accepted for publication on A&A, 14 pages of main text with 5 figures, and 11 pages of appendices A, B, C, D, E and F with 13 figure