Improvements to the "sket bottle": a simple manual device for sampling small crustaceans from marine caves and other cryptic habitats

Dark littoral submarine caves can act as enclaves of the deep aphotic zone in shallow coastal areas, and their survey has revealed the existence of a very particular fauna of specialized and poorly known organisms among which crustaceans are particularly well represented. In these particular habitats, the use of conventional sampling techniques, such as hand nets, is often not recommended since they disturb bottom sediments causing hazardous situations to scientific divers. The use of baited traps, while technically possible, is not. always practical is such remote habitats. The present work describes a simple and inexpensive manual device that can be operated by divers ill submarine caves and other cryptic habitats to recurrently catch small motile organisms such as mysid crustaceans, caridean shrimps, or even gobiid fishes. This small suction bottle derived and improved from the original "Sket bottle" design considerably reduces the risks of disturbing the cave's bottom sediment and can be easily operated using a single hand. The described sampling device can also be easily used outside caves, in a variety of particular habitats, e.g., rubble filled bottoms, branching coral reefs, cracks, and small holes on rocky surfaces, in which small motile organisms usually escape from traditional sampling gears, e.g., fishnets and traps, or simply go unnoticed by researchers during sampling.info:eu-repo/semantics/publishedVersio

Brought more than twice: the complex introduction history of the red swamp crayfish into Europe

One of the biggest challenges in understanding and managing biological invasions is the identification of the routes of introduction. This information is often incomplete because of unnoticed, unreported and, sometimes, illegal translocations. Reports on the introduction of the red swamp crayfish (Procambarus clarkii) into Europe describe that it was introduced for the first time to southern Spain (1973 and 1974) from Louisiana; from there, it rapidly spread throughout several European countries. While other importation events and pathways of introduction have been suggested in the literature, there is no evidence that these led to wild populations in Europe. Our present study suggests two additional introduction routes into Europe from non-European areas where the red swamp crayfish had previously been reported. By using mtDNA, we found a shared haplotype between the Lake Naivasha in Kenya and Western Europe, as well as another between either western United States or Asia, and Malta. These findings support historical reports found in the literature for the former case and also point towards pet trade as driver of new introductions for the latter, thus highlighting the complex introduction history of the red swamp crayfish populations in Europe.Andalusian Government [RNM-936]PICS [PIC2015FR4, PICS07360

Comparative feeding rates of native and invasive ascidians

Ascidians have a recent history of species introductions globally, often with strong ecological impacts. Comparisons of per capita effects of invaders and comparable natives are useful to assess such impacts. Here, we explore ingestion rates (IR) and clearance rates (CR) of Ciona intestinalis and Ciona robusta, co-occurring native and non-native ascidians, respectively, from Brittany, France. IR was positively related to food concentration, with the invader responding more strongly to increasing food concentration. CR also differed by species, with the invader demonstrating higher values. C. robusta exhibited a higher functional response (Type I) than did C. intestinalis (Type II). Relative impact measured using seasonal abundance and IR revealed that C. robusta has a much greater impact than C. intestinalis at all food concentrations tested, though the former has a constrained distribution which limits its regional impact. Nevertheless, when abundant, we expect C. robusta to exert a greater impact on algal foods

Invasion of fluvial ecosystems by red swamp crayfish, Procambarus clarkii

Trabajo presentado en el International Symposium on Conservation of Native European Freshwater Crayfish, celebrado en Olot (Girona) del 23 al 25 de septiembre de 2015.Biological invasions are one of the biggest threats to biodiversity and conservation of native species, playing a key role in global change. To try to control them, it is important to understand two types of phenomena: mechanisms that allow a species to successfully establish in a new environment, and the ecological, evolutionary and socio-economic consequences of an invasion. We will address both types of phenomena using a multidisciplinary approach and multi-scale research, employing as study model an invasive species of global importance, which has invaded many inland waters: the red swamp crayfish, Procambarus clarkii. In its native area, this species mainly lives in marshes; nevertheless, it has been able to invade a new environment, streams, in invaded areas (in our case: West Andalusia in South Western Spain). Since its introduction over 40 years ago, this invasion is causing serious damage to native species like the European freshwater crayfish, Austropotamobius pallipes. We have five main objectives in this study: (1) to compare the genetic diversity of invasive populations with the native area, identifying patterns of introduction, propagation and gene flow; (2) to determine what mechanisms (at the level of gene expression in different tissues) allow P. clarkii to adapt to new conditions or environmental stress: identify genes and loci responsible for local adaptation; (3) to explore the ecological effects of P. clarkii in streams, in terms of structure and ecosystem functioning, and interactions with populations of native crayfish (A. pallipes) and native amphibians and fishes. To do this, we will compare the ecological effects of stream populations (which have potentially evolved in response to the new environment) with those of founding populations from marshes; (4) to compare the ecological effects of P. clarkii with those of A. pallipes and determine whether the invasive species is occupying the same ecological niche as the native species which has displaced; and (5) to examine the prevalence of Aphanomyces astaci in the red swamp crayfish, given that this invasive species is a chronic carrier of the crayfish plague, aphanomycosis, causing the death of European freshwater crayfish; and that of the fungus that causes chytridiomycosis in amphibians. In this project, we expect to find mechanisms that allow the red swamp crayfish to successfully establish in a new environment and the consequences that this entails for native species.Peer reviewe

Invasión de ecosistemas fluviales por cangrejo rojo americano, Procambarus clarkii

Trabajo presentado en las Jornadas españolas sobre conservación del cangrejo de río autóctono, celebradas el 21 y 22 de septiembre de 2015.Las invasiones biológicas son una de las mayores amenazas para la biodiversidad y conservación de las especies autóctonas, jugando un papel clave en el cambio global. Para intentar controlarlas, es importante entender dos tipos de fenómenos: los mecanismos que permiten a una especie establecerse con éxito en un nuevo ambiente, y las consecuencias ecológicas, evolutivas y económicas de una invasión. Nosotros abordaremos ambos tipos de fenómenos usando un enfoque multidisciplinar y un estudio multiescala, empleando como modelo de estudio una de las especies invasoras con mayor importancia global, la cual ya ha invadido la mayoría de aguas continentales: el cangrejo rojo americano, Procambarus clarkii. En su área nativa, esta especie vive principalmente en marismas; sin embargo, en las áreas invadidas ha sido capaz de invadir un nuevo ambiente, los arroyos, (en nuestro caso: el oeste de Andalucía en el suroeste de España). Desde su introducción hace unos 40 años, esta invasión está causando importantes daños a las especies autóctonas como el cangrejo de río, Austropotamobius pallipes. En este estudio, nos planteamos los siguientes cinco objetivos: (1) comparar la diversidad genética de las poblaciones invasoras con las de las áreas nativas, identificando patrones de introducción, propagación y flujo génico; (2) determinar qué mecanismos (a nivel de expresión de genes en diferentes tejidos) permiten que P. clarkii se adapte a nuevas condiciones o estrés ambiental: identificando genes y loci responsables para la adaptación local; (3) explorar los efectos ecológicos de P. clarkii en arroyos, en términos de estructura y funcionamiento del ecosistema, además de las interacciones con poblaciones nativas, tanto de cangrejo de río (A. pallipes) como de anfibios y peces autóctonos. Para hacer esto, compararemos los efectos ecológicos de las poblaciones de arroyo (las cuales potencialmente han evolucionado en respuesta a nuevos ambientes) con aquellas de poblaciones fundadoras desde las marismas; (4) comparar los efectos ecológicos de P. clarkii con aquellos de A. pallipes y determinar si la especie invasora está ocupando el mismo nicho ecológico que la especie nativa a la cual ha desplazado; y (5) examinar la prevalencia de Aphanomyces astaci en el cangrejo rojo americano, dado que esta especie invasora es portadora de la peste del cangrejo, afanomicosis, causando la muerte del cangrejo de río autóctono; y también la del hongo que causa la quitridiomicosis en anfibios. Con este proyecto, pretendemos dilucidar los mecanismos que permiten al cangrejo rojo americano establecerse exitosamente en un nuevo ambiente y las consecuencias que esto conlleva para las especies autóctonas.Peer reviewe

Community-Level Responses to Iron Availability in Open Ocean Plankton Ecosystems

Predicting responses of plankton to variations in essential nutrients is hampered by limited in situ measurements, a poor understanding of community composition, and the lack of reference gene catalogs for key taxa. Iron is a key driver of plankton dynamics and, therefore, of global biogeochemical cycles and climate. To assess the impact of iron availability on plankton communities, we explored the comprehensive bio-oceanographic and bio-omics data sets from Tara Oceans in the context of the iron products from two state-of-the-art global scale biogeochemical models. We obtained novel information about adaptation and acclimation toward iron in a range of phytoplankton, including picocyanobacteria and diatoms, and identified whole subcommunities covarying with iron. Many of the observed global patterns were recapitulated in the Marquesas archipelago, where frequent plankton blooms are believed to be caused by natural iron fertilization, although they are not captured in large-scale biogeochemical models. This work provides a proof of concept that integrative analyses, spanning from genes to ecosystems and viruses to zooplankton, can disentangle the complexity of plankton communities and can lead to more accurate formulations of resource bioavailability in biogeochemical models, thus improving our understanding of plankton resilience in a changing environment

Population structure and life history of Hemimysis margalefi (Crustacea: Mysidacea), a "thermophilic" cave-dwelling species benefiting from the warming of the NW Mediterranean

Dark submarine caves are an extreme and fragmented habitat in which mysids (Crustacea: Mysidacea) of the genus Hemimysis can be found. Hemimysis speluncola has long been the dominant mysid species of the NW Mediterranean caves, but with the recent warming of this region, its congener H. margalefi has replaced it. Nothing is known about the biology and ecology of H. margalefi and here, we provide the first information about its demographic structure and reproductive biology in a cave recently affected by the species shift. We conducted monthly sampling for 4 yr in the Jarre Island cave, near Marseilles (France), where a population of H. margalefi established itself in the late 1990s. Population dynamics were followed by monitoring length-frequency histograms and the influence of temperature on several life-history traits was investigated. H. margalefi reproduces all year round and the brood size depends on female size. Recruitment is discontinuous and occurs when post-nauplioid larvae are 1.3 to 2.0 mm long. Four annual and overlapping cohorts (about 6 mo long) underline more intensive periods of breeding. Females can produce up to 2 successive cohorts during their life. Whatever the maturity stage, females present larger sizes than males. Seasonal variations of water temperature have a strong influence on different life-history traits of H. margalefi, such as mean sizes of the different cohorts, length of intermoult stages and growth rate. Some population dynamics features of H. margalefi are a further indication that it is a species with an affinity for warm water, which has recently benefited from the warmer conditions in the NW Mediterranean, colonising new areas and replacing its congener H. speluncola in most of its former range

The Possible Failed Pre-Linnaean Introduction in the Mediterranean Sea: An Archival Case Study of the Brown Mussel Perna perna

International audienceMost species arriving from a donor to a recipient area do not succeed in establishing long-lasting self-sustaining populations. However, successful introductions are far better documented than those that failed, especially those occurring before or near the advent of the Linnaean binomial nomenclature. We report here an introduction from the mid-18th century (possibly in 1750 or 1751) of an exotic mussel transported as fouling on ship hulls from the western coast of Morocco (Atlantic Ocean) to the port of Marseilles (Mediterranean Sea). The exotic mussel, which survived several years, has been identified as probably being the brown mussel, Perna perna, a species with warm-water affinities, which much later became invasive in several areas of the world ocean. The documents of the 18th and early 19th century, which mentioned the event, held ‘the curious’ and ‘amateurs’, who harvested the mussels, responsible for its extirpation. More realistically, it is hypothesised that the mussel population did not survive the return of severe cold weather conditions, after a few relatively mild decades, in the context of the Little Ice Age (LIA). These conclusions were deduced from historical data and are therefore open to discussion

Compared stress tolerance to short-term exposure in native and invasive tunicates from the NE Atlantic: when the invader performs better

International audienceThe combined impact of invasive species and climate change threatens natural systems worldwide, often facilitating the expansion of harmful invasive species. It is imperative to understand the mechanisms behind why species become invasive and widespread. Traditionally, it is thought that invasive species have greater tolerances to a wider array of environmental conditions than natives. We, therefore, tested the hypothesis that invasive species are more tolerant to the effects of short-term exposure to temperature and salinity stress. Using unifactorial experiments, we compared the tolerances of two common fouling NE Atlantic ascidians, the native Ciona intestinalis and the invasive Styela clava, to increased temperature and decreased salinity. We measured lethal and behavioural responses affecting 50% of populations to give an indication of the tolerance limits for temperature (LT50) and salinity (EC50), and respiration rate to give an indication of the change in metabolic response. The invasive S. clava was more tolerant to increased stress (LT50 = 29.5 °C, EC50 = 19.5) compared with C. intestinalis (LT50 = 27.0 °C, EC50 = 22.7), whereas both species displayed similar metabolic responses observed through increased respiration rates. This study is among the first to experimentally determine limits for temperature and hyposalinity stress for either species and supports the hypothesis that the invader performs better under extreme conditions. Future environmental changes caused by events such as heat waves and climate change could push species to the edge of their physiological limits, potentially facilitating competitive shifts between native and invasive species

Local variation within marinas: Effects of pollutants and implications for invasive species

International audienceUrban structures like marinas are dominant features of our coasts, often hotspots for invasive species. The processes that govern the distribution of invasive species within and between marinas are not well understood. We therefore investigated the impacts of local-scale variability within and between marinas, analysing fouling communities at two zones (inner and outer) within three close marinas in accordance with pollutants recorded in the water and sediment. Communities varied between zones, however no significant differences in abundances of invasive species was recorded. The inner zones contained higher levels of copper and other pollutants and were correlated with lower biodiversity and abundances of many species in comparison to the outer zones. Only the native Ascidiella aspersa was found in greater abundances in the inner zones. This local-scale variability and how it impacts biodiversity is important for consideration for coastal managers in mitigating the build-up of pollutants and spread of invasive species