Absolute measurement of the nitrogen fluorescence yield in air between 300 and 430 nm

The nitrogen fluorescence induced in air is used to detect ultra-high energy cosmic rays and to measure their energy. The precise knowledge of the absolute fluorescence yield is the key quantity to improve the accuracy on the cosmic ray energy. The total yield has been measured in dry air using a 90Sr source and a [300-430 nm] filter. The fluorescence yield in air is 4.23 $\pm$ 0.20 photons per meter when normalized to 760 mmHg, 15 degrees C and with an electron energy of 0.85 MeV. This result is consistent with previous experiments made at various energies, but with an accuracy improved by a factor of about 3. For the first time, the absolute continuous spectrum of nitrogen excited by 90Sr electrons has also been measured with a spectrometer. Details of this experiment are given in one of the author's PhD thesis [32].Comment: accepted for publication in NIM

Composition of fish communities in macrotidal salt marshes of the Mont Saint-Michel bay (France)

At least 100 fish species are known to be present in the intertidal areas (estuaries, mudflats and salt marshes) of Mont Saint-Michel Bay. These and other comparable shallow marine coastal waters, such as estuaries and lagoons, play a nursery role for many fish species. However, in Europe little attention has been paid to the value of tidal salt marshes for fishes. Between March 1996 and April 1999, 120 tides were sampled in a tidal creek. A total of 31 species were caught. This community was largely dominated by mullets (Liza ramada represent 87% of the total biomass) and sand gobies(Pomatoschistus minutus and P. lozanoi represent 82% of the total numbers). These species and also Gasterosteus aculeatus, Syngnathus rostellatus, Dicentrarchus labrax, Mugil spp., Liza aurata and Sprattus sprattus were the most frequent species (>50% of monthly frequency of occurrence). In Europe, salt marshes and their creeks are flooded only during high spring tides. So, fishes only invade this environment during short immersion periods, and no species can be considered as marsh resident. But, the salt marsh was colonized by fish every time the tide reached the creek, and during the short time of flood, dominant fishes fed actively and exploited the high productivity. Nevertheless, this study shows that there is little interannual variation in the fish community and there are three ‘ seasons ’ in the fish fauna of the marsh. Marine straggler and marine estuarine dependent species colonize marshes between spring (recruitment period in the bay) and autumn before returning into deeper adjacent waters. Estuarine fishes are present all year round with maximum abundances in the end of summer. The presence of fishes confirms that this kind of wetland plays an important trophic and nursery role for these species. Differences in densities and stages distribution of these species into Mont Saint-Michel systems (tidal mudflats, estuaries and tidal salt marshes) can reduce the trophic competition

Marked length spectrum rigidity for Anosov surfaces

Let $\Sigma$ be a smooth closed oriented surface of genus $\geq 2$. We prove that two metrics on $\Sigma$ with same marked length spectrum and Anosov geodesic flow are isometric via an isometry isotopic to the identity. The proof combines microlocal tools with the geometry of complex curves.Comment: v2: We correct and simplify the proof of Lemma 3.11. v3: Added a corollary on the centralizer of Anosov geodesic flows. 19 pages, 1 figur

Le marais doux endigué de Bourgneuf-Machecoul (Pays de Loire) Premier éléments de connaissance du peuplement piscicole. Relation ichtyofaune-habitat et problèmes majeurs de gestion (Maroc)

Le peuplement ichtyologique d'un marais littoral endigué, géré en eau douce, a été étudié sur une zone de 2 700 ha, située au nord du marais Breton-Vendéen (Loire-Atlantique, France). Le réseau hydraulique, qui représente près de 16 % de cette surface, se compose, d'environ 91 m de linéaire de fossés par ha (en tout 234 km) et de bassins présents uniquement dans la partie d'origine salicole du marais. Les hauteurs d'eau, l'envasement et le recouvrement par la végétation aquatique dépendent de la gestion humaine et sont très variables (moyennes respectives : 42 cm; 43 cm; 70 %). Cela se traduit par un morcellement spatial de l'habitat pour les poissons. La stratégie d'échantillonnage adoptée, qui tient compte de cette hétérogénéité, a permis de décrire un peuplement comportant 21 espèces. Dominé par les poissons-chats et par les anguilles, ce dernier est caractéristique de la zone à brèmes des cours d'eau. Les abondances sont relativement élevées (en moyenne 315 kg/ha et 11 460 poissons/ha), mais elles sont très hétérogènes. L'évolution qualitative et quantitative de la répartition spatio-temporelle est décrite à l'aide d'une analyse factorielle des correspondances portant sur 74 échantillons prélevés par pêche électrique entre 1987 et 1989. Bien que l'approche de ce milieu soit complexe et les références bibliographiques relativement rares, l'analyse des premières données permet d'ores et déjà d'identifier quelques problèmes de gestion ayant des répercussions directes sur le peuplement piscicole de cette zone.The dammed up marshes of the French Atlantic coast cover about 200 000 ha between River Vilaine and the « Bassin d'Arcachon ». Eighty eight % are managed with freshwater. They constitute original environments initially created for agriculture or for salt production, and they are now threatened by land abandonment within the next decade. Concurrently to aquaculture (in created or existing ponds), exploitation of the fish stocks in the ditchweb is likely to encourage a diversification of agricultural activities. Unfortunately, bibliographic analysis reveals the relative scarceness of research about sampling methods and qualitative or quantitative characteristics of these fish communities. This is quite surprising considering the importance of the ditchwed of this kind of environment outlined by several authors. In the Netherlands, BELTMANN (1984) assessed that there is a total of 400 000 km of ditches. In France, the littoral dyked marshes of the Atlantic coast couid comprise 20 000 km of ditches and about 24 000 ha of open water. The present work provides for the first data on the fish community of Bourgneuf marsh.The northern part of the marsh of Bourgneuf, 2 700 ha provided whith f reshwater, contains nearly every kind of landscapes found throughout the whole Breton-Vendéen marsh. The pattern of the ditch network strongly changes from a zone to another (fig.1) : presence of former salt pans in the western part, regular geometric shapes in the recently created polders next to the River Falleron, irregular ditchweb pattern in the eastern part. The average density of the ditch network is 91 m of ditches per ha, totalizing 234 km in the study area. The total surface of open water, composed of ditches and basins (former salt pans), covers 411 ha (over 15 % of the study site). Diversity of ditch types occurs at fine scales (<1 000 m2), they vary according to their widths (0,3 to 7 m), depths (average, 42 cm; SD, 20,4), thickness of silt layer (average, 43 cm; SD, 42) and their hydrophyte vegetation cover (average, 70 %; SD, 60 %). As a consequence of this heterogeneity, available habitats are scattered over the marsh (mosaïc distribution). A nested sampling (FRONTIER, 1983) was carried out to take into account this high heterogeneity : 5 sampling areas were selected randomly. In each one, 3 to 5 ditches were chosen according to their characteristics (see above). Sampling stations were delimited by 2 stop nets (5 mm mesh) settled 30 m apart, in order to avoid fish migration. Field work was conducted using « Heron » electric fishing material (see LAMARQUE et al., 1978). In each ditch-section, we carried out as many successive catches as necessary to apply the maximum likelihood weighted estimation method of CARLE and STRUB (1978). Nine to 19 stations were sampled at 5 periods, between 1987 and 1989. A total of 74 samples were collected.The fish community was composed of 21 species (table 1) and corresponded to the bream zone of Verneaux's classification (1977). The densities and biomass were quite high (on average 315 kg/ha and 11 460 fishes/ha) but very variable (0 to 2 120 kg/ ha and 0 to 39 300 fishes/ha). The catfish, Ictalurus nebulosus (170 kg/ha), the eel, Anguilla anguilla (47 kg/ha) and the tench, Tinca tinca (28 kg/ha), represented on average 77,5 % of the standing crop, but their spatial distribution was very irregular. These estimates are assumed to be reliable considering that the data used for the calculations were provided by a sampling design which permits to respect the basic assumptions of the removal method. (f) The population size could only change because of the fishings (no migration because of the stop nets; no recruitment/death because of the short duration of the fishing sequences). (ii) The standard sampling design permitted to reduce the variations of the catch probabilities between the successive removals. Several studies have shown that this removal method under-estimates by about 20 % the true size of the fish populations (e. g. BOHLIN and SUNDSTROM, 1977; MAHON, 1980). But they were based on Zippin's method, and the estimator of CARLE and STRUB (1978), that we used, was shown to be more robust (COWX, 1983; GERDEAUX, 1987). Nevertheless, we assume that the values presented in this paper provide for an approached information on the sizes of the studied fish populations.To assess the fish-habitat relationship, a correspondence analysis (fig. 2) was performed on the 74 samples X 17 species matrix (excluding the sticklebacks, Gaslerosteus aculeatus and Pungitius pungitius, which population size estimations failed because of their low catchabilities). Four groups of samples were ordinated according to their specific richness and the species they contained. Several habitat parameters were projected on F1-F2 factorial map (fig. 3). Hydrophyte cover, thickness of silt layer, water depth (fig. 3 and 4), which are directly controlled by human maintenance, appeared to be the major structuring habitat parameters for the fish community. In the deepest and less silted stations, the communities were rich (on average 11 species; group 4, fig. 2). Predators such as pike-perch, Stizostedion lucioperca, and perch, Perca fluviatilis, occurred, and cold water species were found, such as minnow, Phoxinus phoxinus, or chub, Leuciscus cephalus. When the silt layer was thicker and the water level was intermediate, the specific richness decreased (average, 6,2 species) and the community was either dominated by the cattish (group 2, fig. 2) or by the rudd, Scardinius erythrophtalmus (group 3, fig. 2), according to the importance of the aquatic vegetation cover. Habitats with thickest silt layers, shallowest waters and maximum aquatic vegetation cover contained the poorest communities (average 3,9 species) dominated by eel (group 1, fig. 2). There is also evidence that the diversity of the community has progressively decreased since 1987 (fig. 5). The most stenothermous species disappeared, and the importance of the catfish increased : it doubled between May 1987 and September 1989 (fig. 6). Although the eel is the species most adapted to this environment, we emphasize the diminution of its biomass (fig. 7). These phenomena could be partly due to the climate (cold winter in 1987, important swelling in January 1988 and 2 droughts in summers 1988 and 1989). But they are mainly caused by the water management policy which is intended to favour agriculture by keeping stable water levels (evacuation of swellings) and by preventing the freshwater part from the marine influence (collective sluice gates). This does mot permit an optimal breeding of the species that have to spawn on flooded meadows, neither a proper colonisation of the marsh by elvers

Avoidance of Protein Fold Disruption in Natural Virus Recombinants

With the development of reliable recombination detection tools and an increasing number of available genome sequences, many studies have reported evidence of recombination in a wide range of virus genera. Recombination is apparently a major mechanism in virus evolution, allowing viruses to evolve more quickly by providing immediate direct access to many more areas of a sequence space than are accessible by mutation alone. Recombination has been widely described amongst the insect-transmitted plant viruses in the genus Begomovirus (family Geminiviridae), with potential recombination hot- and cold-spots also having been identified. Nevertheless, because very little is understood about either the biochemical predispositions of different genomic regions to recombine or what makes some recombinants more viable than others, the sources of the evolutionary and biochemical forces shaping distinctive recombination patterns observed in nature remain obscure. Here we present a detailed analysis of unique recombination events detectable in the DNA-A and DNA-A-like genome components of bipartite and monopartite begomoviruses. We demonstrate both that recombination breakpoint hot- and cold-spots are conserved between the two groups of viruses, and that patterns of sequence exchange amongst the genomes are obviously non-random. Using a computational technique designed to predict structural perturbations in chimaeric proteins, we demonstrate that observed recombination events tend to be less disruptive than sets of simulated ones. Purifying selection acting against natural recombinants expressing improperly folded chimaeric proteins is therefore a major determinant of natural recombination patterns in begomoviruses

Recombination in Eukaryotic Single Stranded DNA Viruses

Although single stranded (ss) DNA viruses that infect humans and their domesticated animals do not generally cause major diseases, the arthropod borne ssDNA viruses of plants do, and as a result seriously constrain food production in most temperate regions of the world. Besides the well known plant and animal-infecting ssDNA viruses, it has recently become apparent through metagenomic surveys of ssDNA molecules that there also exist large numbers of other diverse ssDNA viruses within almost all terrestrial and aquatic environments. The host ranges of these viruses probably span the tree of life and they are likely to be important components of global ecosystems. Various lines of evidence suggest that a pivotal evolutionary process during the generation of this global ssDNA virus diversity has probably been genetic recombination. High rates of homologous recombination, non-homologous recombination and genome component reassortment are known to occur within and between various different ssDNA virus species and we look here at the various roles that these different types of recombination may play, both in the day-to-day biology, and in the longer term evolution, of these viruses. We specifically focus on the ecological, biochemical and selective factors underlying patterns of genetic exchange detectable amongst the ssDNA viruses and discuss how these should all be considered when assessing the adaptive value of recombination during ssDNA virus evolution

Characterization Of Epoxy-Coated Oxide Films Using Acoustic Microscopy

An adhesive joint consisting of aluminum adherends bonded with an epoxy adhesive is composed of three main layers. The adherends are usually a few millimeters thick with a layer of epoxy adhesive between one and three hundred microns thick between them. The surfaces of the adherends are typically pre-treated to produce a thin film of porous aluminum oxide, which has a honeycomb-like structure. The epoxy adhesive may then penetrate into these honeycomb cells or pores. The resulting layer between the adhesive and adherend is therefore a micro-composite and it is typically of the order of one micron in thickness. The use of the surface pre-treatment is a major factor in increasing the durability of the adhesive joint when it is exposed to water. Additionally, joints which have been in use for some time, especially ones which have been subject to environmental attack, usually experience a failure along the plane of this film. Therefore, characterization of this epoxy/oxide interlayer is very important in understanding adhesive joints and how they are affected by environmental factors. Unfortunately, not much is known about their mechanical properties

Search for the disappearance of muon antineutrinos in the NuMI neutrino beam

We report constraints on muon antineutrino oscillation parameters that were obtained by using the two MINOS detectors to measure the 7% antineutrino component of the NuMI neutrino beam. In the Far Detector, we select 130 events in the charged-current muon antineutrino sample, compared to a prediction of 136.4 +/- 11.7(stat) ^{+10.2}_{-8.9}(syst) events under the assumption |dm2bar|=2.32x10^-3 eV^2, snthetabar=1.0. A fit to the two-flavor oscillation approximation constrains |dm2bar|&lt;3.37x10^-3 eV^2 at the 90% confidence level with snthetabar=1.0

Impact of sheep grazing on juvenile sea bass, Dicentrarchus labrax L., in tidal salt marshes

The diet of young of the year sea bass, Dicentrarchus labrax L., from sheep grazed and ungrazed tidal salt marshes were com-pared qualitatively and quantitatively in Mont Saint-Michel Bay. In areas without grazing pressure, the vegetation gradient changes from a pioneer Puccinellia maritima dominated community at the tidal ¯at boundaries through a Atriplex portulacoides dominated community in the middle of the marsh to a mature Elymus pungens dominated community at the landward edge. The A. portula-coides community is highly productive and provides important quantities of litter which provides a habitat and good supply to substain high densities of the detrivorous amphipod Orchestia gammarellus. In the grazed areas, the vegetation is replaced by P. maritima communities, a low productive grass plant, and food availability and habitat suitability are reduced for O. gammarellus. Juvenile sea bass colonise the salt marsh at ¯ood during 43% of the spring tides which inundate the salt marsh creeks. They forage inside the marsh and feed mainly on O. gammarellus in the ungrazed marshes. In grazed areas, this amphipod is replaced by other species and juvenile sea bass consume less food from the marsh. This illustrates a direct effect of a terrestrial herbivore on a coastal food web, and suggests that management of salt marsh is complex and promotion of one component of their biota could involve reductions in other species