A 3D mechanical model of the early mammalian embryo

The early development of the mammalian embryo leads to the formation of a structure composed by an outer layer of polarized cells surrounding an inner mass of nonpolarized cells. Experimental biology has shown that this organization results from changes in cell polarity, cell shape and intercellular contacts at the 8 and 16-cell stages. In order to examine how the physical properties of embryo cells (adhesion, cortical tension) influence the organization of the cells within the embryo, our team has developed a 3D mechanical model of the dividing early embryo, based on cellular Potts models. In this paper we will present the principles of our simulations, the methodology used and we will show that a very simple mechanical model can reproduce the main structural features (geometry, cell arrangement) of the mammalian embryo during its early developmental stages, up to the 16-cell stage

Active Control of Silicon Nanotweezers Detects Enzymatic Reaction at the Molecular Level

International audienceThis work achieved the control of micromachined tweezers for the enhancement of the sensing of DNA molecules and related enzymatic reactions. The mechanical stiffness of the silicon nanotweezers is decreased by feedback design and the sensitivity of the system is drastically improved

Closed-loop Control of Silicon Nanotweezers for Improvement of Sensitivity to Mechanical Stiffness Measurement and Bio-Sensing on DNA Molecules

International audienceIn this work we show that implementation of closed loop control to silicon nanotweezers improves the sensitivity of the tool for mechanical characterizations of biological molecules. Micromachined tweezers have already been used for the characterizations of mechanical properties of DNA molecules as well as for the sensing of enzymatic reactions on DNA bundle. However the resolution of the experiments does not allow the sensing on single molecules. Hereafter we show theoretically and experimentally that, reducing the resonance frequency of the system by the implementation of a state feedback, the sensitivity to stiffness variation is enhanced. Such improvement leads to better resolution for detection of enzymatic reactions on DNA

Bistable Cell Fate Specification as a Result of Stochastic Fluctuations and Collective Spatial Cell Behaviour

BACKGROUND: In culture, isogenic mammalian cells typically display enduring phenotypic heterogeneity that arises from fluctuations of gene expression and other intracellular processes. This diversity is not just simple noise but has biological relevance by generating plasticity. Noise driven plasticity was suggested to be a stem cell-specific feature. RESULTS: Here we show that the phenotypes of proliferating tissue progenitor cells such as primary mononuclear muscle cells can also spontaneously fluctuate between different states characterized by the either high or low expression of the muscle-specific cell surface molecule CD56 and by the corresponding high or low capacity to form myotubes. Although this capacity is a cell-intrinsic property, the cells switch their phenotype under the constraints imposed by the highly heterogeneous microenvironment created by their own collective movement. The resulting heterogeneous cell population is characterized by a dynamic equilibrium between "high CD56" and "low CD56" phenotype cells with distinct spatial distribution. Computer simulations reveal that this complex dynamic is consistent with a context-dependent noise driven bistable model where local microenvironment acts on the cellular state by encouraging the cell to fluctuate between the phenotypes until the low noise state is found. CONCLUSIONS: These observations suggest that phenotypic fluctuations may be a general feature of any non-terminally differentiated cell. The cellular microenvironment created by the cells themselves contributes actively and continuously to the generation of fluctuations depending on their phenotype. As a result, the cell phenotype is determined by the joint action of the cell-intrinsic fluctuations and by collective cell-to-cell interactions

Slitless spectrophotometry with forward modelling: principles and application to atmospheric transmission measurement

In the next decade, many optical surveys will aim to tackle the question of dark energy nature, measuring its equation of state parameter at the permil level. This requires trusting the photometric calibration of the survey with a precision never reached so far, controlling many sources of systematic uncertainties. The measurement of the on-site atmospheric transmission for each exposure, or on average for each season or for the full survey, can help reach the permil precision for magnitudes. This work aims at proving the ability to use slitless spectroscopy for standard star spectrophotometry and its use to monitor on-site atmospheric transmission as needed, for example, by the Vera C. Rubin Observatory Legacy Survey of Space and Time supernova cosmology program. We fully deal with the case of a disperser in the filter wheel, which is the configuration chosen in the Rubin Auxiliary Telescope. The theoretical basis of slitless spectrophotometry is at the heart of our forward model approach to extract spectroscopic information from slitless data. We developed a publicly available software called Spectractor (https://github.com/LSSTDESC/Spectractor) that implements each ingredient of the model and finally performs a fit of a spectrogram model directly on image data to get the spectrum. We show on simulations that our model allows us to understand the structure of spectrophotometric exposures. We also demonstrate its use on real data, solving specific issues and illustrating how our procedure allows the improvement of the model describing the data. Finally, we discuss how this approach can be used to directly extract atmospheric transmission parameters from data and thus provide the base for on-site atmosphere monitoring. We show the efficiency of the procedure on simulations and test it on the limited data set available.Comment: 30 pages, 36 figures, submitted to Astronomy and Astrophysic

De 20 000 à 18 000 BP en Quercy : apports de la séquence du Cuzoul de Vers à la compréhension de l'évolution des comportements socio-économiques entre Solutréen récent et Badegoulien

Essai de synthèse des travaux menés autour du gisement du Cuzoul de Vers (Lot)

A divergent heritage for complex organics in Isheyevo lithic clasts

Primitive meteorites are samples of asteroidal bodies that contain a high proportion of chemically complex organic matter (COM) including prebiotic molecules such as amino acids, which are thought to have been delivered to Earth via impacts during the early history of the Solar System. Thus, understanding the origin of COM, including their formation pathway(s) and environment(s), is critical to elucidate the origin of life on Earth as well as assessing the potential habitability of exoplanetary systems. The Isheyevo CH/CBb carbonaceous chondrite contains chondritic lithic clasts with variable enrichments in 15N believed to be of outer Solar System origin. Using transmission electron microscopy (TEM-EELS) and in situ isotope analyses (SIMS and NanoSIMS), we report on the structure of the organic matter as well as the bulk H and N isotope composition of Isheyevo lithic clasts. These data are complemented by electron microprobe analyses of the clast mineral chemistry and bulk Mg and Cr isotopes obtained by inductively coupled plasma and thermal ionization mass spectrometry, respectively (MC-ICPMS and TIMS). Weakly hydrated (A) clasts largely consist of Mg-rich anhydrous silicates with local hydrated veins composed of phyllosilicates, magnetite and globular and diffuse organic matter. Extensively hydrated clasts (H) are thoroughly hydrated and contain Fe-sulfides, sometimes clustered with organic matter, as well as magnetite and carbonates embedded in a phyllosilicate matrix. The A-clasts are characterized by a more 15N-rich bulk nitrogen isotope composition (δ15N = 200–650‰) relative to H-clasts (δ15N = 50–180‰) and contain extremely 15N-rich domains with δ15N 15N-rich domains show that the lithic clast diffuse organic matter is typically more 15N-rich than globular organic matter. The correlated δ15N values and C/N ratios of nanoglobules require the existence of multiple organic components, in agreement with the H isotope data. The combined H and N isotope data suggest that the organic precursors of the lithic clasts are defined by an extremely 15N-poor (similar to solar) and D-rich component for H-clasts, and a moderately 15N-rich and D-rich component for A-clasts. In contrast, the composition of the putative fluids is inferred to include D-poor but moderately to extremely 15N-rich H- and N-bearing components. The variable 15N enrichments in H- and A-clasts are associated with structural differences in the N bonding environments of their diffuse organic matter, which are dominated by amine groups in H-clasts and nitrile functional groups in A-clasts. We suggest that the isotopically divergent organic precursors in Isheyevo clasts may be similar to organic moieties in carbonaceous chondrites (CI, CM, CR) and thermally recalcitrant organic compounds in ordinary chondrites, respectively. The altering fluids, which are inferred to cause the 15N enrichments observed in the clasts, may be the result of accretion of variable abundances of NH3 and HCN ices. Finally, using bulk Mg and Cr isotope composition of clasts, we speculate on the accretion regions of the various primitive chondrites and components and the origin of the Solar System’s N and H isotope variability

SN 2009ip at late times - an interacting transient at+2 years

We present photometric and spectroscopic observations of the interacting transient SN 2009ip taken during the 2013 and 2014 observing seasons. We characterize the photometric evolution as a steady and smooth decline in all bands, with a decline rate that is slower than expected for a solely Co-56-powered supernova at late phases. No further outbursts or eruptions were seen over a two year period from 2012 December until 2014 December. SN 2009ip remains brighter than its historic minimum from pre-discovery images. Spectroscopically, SN 2009ip continues to be dominated by strong, narrow (less than or similar to 2000 km s(-1)) emission lines of H, He, Ca, and Fe. While we make tenuous detections of [Fe II] lambda 7155 and [O I] lambda lambda 6300, 6364 lines at the end of 2013 June and the start of 2013 October, respectively, we see no strong broad nebular emission lines that could point to a core-collapse origin. In general, the lines appear relatively symmetric, with the exception of our final spectrum in 2014 May, when we observe the appearance of a redshifted shoulder of emission at +550 km s(-1). The lines are not blueshifted, and we see no significant near-or mid-infrared excess. From the spectroscopic and photometric evolution of SN 2009ip until 820 d after the start of the 2012a event, we still see no conclusive evidence for core-collapse, although whether any such signs could be masked by ongoing interaction is unclear

Observation of Microlensing towards the Galactic Spiral Arms: EROS II 3 year survey

We present an analysis of the light curves of 9.1 million stars observed during three seasons by EROS (Experience de Recherche d'Objets Sombres), in the Galactic plane away from the bulge. Seven stars exhibit luminosity variations compatible with gravitational microlensing effects due to unseen objects. The corresponding optical depth, averaged over four directions, is tau = 0.45 +0.24 -0.11 x 10^-6. While this value is compatible with expectations from simple galactic models under reasonable assumptions on the target distances, we find an excess of events with short timescale towards the direction closest to the Galactic Centre