Mapping Luminous Blue Compact Galaxies with VIRUS-P: morphology, line ratios and kinematics

[abridged] We carry out an integral field spectroscopy (IFS) study of a sample of luminous BCGs, with the aim to probe the morphology, kinematics, dust extinction and excitation mechanisms of their warm interstellar medium (ISM). IFS data for five luminous BCGs were obtained using VIRUS-P, the prototype instrument for the Visible Integral Field Replicable Unit Spectrograph, attached to the 2.7m Harlan J. Smith Telescope at the McDonald Observatory. VIRUS-P consists of a square array of 247 optical fibers, which covers a 109"x109" field of view, with a spatial sampling of 4.2" and a 0.3 filling factor. We observed in the 3550-5850 Angstrom spectral range, with a resolution of 5 A FWHM. From these data we built two-dimensional maps of the continuum and the most prominent emission-lines ([OII]3727, Hgamma, Hbeta and [OIII]5007), and investigate the morphology of diagnostic emission-line ratios and the extinction patterns in the ISM as well as stellar and gas kinematics. Additionally, from integrated spectra we infer total line fluxes and luminosity-weighted extinction coefficients and gas-phase metallicities. All galaxies exhibit an overall regular morphology in the stellar continuum, while their warm ISM morphology is more complex: in II Zw 33 and Mrk 314, the star-forming regions are aligned along a chain-structure; Haro 1, NGC 4670 and III Zw 102 display several salient features, such as extended gaseous filaments and bubbles. A significant intrinsic absorption by dust is present in all galaxies, the most extreme case being III Zw 102. Our data reveal a manifold of kinematical patterns, from overall regular gas and stellar rotation to complex velocity fields produced by structurally and kinematically distinct components.Comment: Accepted for publication in A&A. 16 pages, 10 figure

Multi-seeded multi-mode formation of embedded clusters in the RMC: Structured star formation toward the south-east boundary

The Rosette Molecular Complex contains embedded clusters with diverse properties and origins. We have previously explored the shell mode of formation in the north (Regions A & B) and the massive concentrations in the ridge (Region C). Here, we explore star formation towards the south of the complex, Region D, based on data from the spatially complete 2 Micron All Sky Survey. We find that stars are forming prolifically throughout this region in a highly structured mode with both clusters and loose aggregates detected. The most prominent cluster (Region D1) lies in the north-center. This cluster is over 20 pc to the south of the Monoceros ridge, the interface of the emerging young OB cluster NGC 2244 with its ambient molecular clouds. In addition, there are several branches stemming from AFGL 961 in Region C and extending to the south-east boundary of the cloud. We invoke a tree model to interpret this pattern, corresponding to probable tracks of abrupt turbulent excitation and subsequent decay. Alternatively, we discuss gravoturbulent collapse scenarios based on numerical simulations. Relative stellar ages and gas flow directions will differentiate between these mechanisms.Comment: 9 figures, the 4th of a series of paper

Mid-IR emission of galaxies in the Virgo cluster and in the Coma supercluster.IV. The nature of the dust heating sources

We study the relationship between the mid-IR (5-18 $\mu$m) emission of late-type galaxies and various other star formation tracers in order to investigate the nature of the dust heating sources in this spectral domain. The analysis is carried out using a sample of 123 normal, late-type, nearby galaxies with available data at several frequencies. The mid-IR luminosity (normalized to the H-band luminosity) correlates better with the far-IR luminosity than with more direct tracers of the young stellar population such as the H$\alpha$ and the UV luminosity. The comparison of resolved images reveals a remarkable similarity in the H$\alpha$ and mid-IR morphologies, with prominent HII regions at both frequencies. The mid-IR images, however, show in addition a diffuse emission not associated with HII regions nor with the diffuse H$\alpha$ emission. This evidence indicates that the stellar population responsible for the heating of dust emitting in the mid-IR is similar to that heating big grains emitting in the far-IR, including relatively evolved stars responsible for the non-ionizing radiation. The scatter in the mid-IR vs. H$\alpha$, UV and far-IR luminosity relation is mostly due to metallicity effects, with metal-poor objects having a lower mid-IR emission per unit star formation rate than metal-rich galaxies. Our analysis indicates that the mid-IR luminosity is not an optimal star formation tracer in normal, late-type galaxies.Comment: accepted for publication on A&

An Aromatic Inventory of the Local Volume

Using infrared photometry from the Spitzer Space Telescope, we perform the first inventory of aromatic feature emission (AFE, but also commonly referred to as PAH emission) for a statistically complete sample of star-forming galaxies in the local volume. The photometric methodology involved is calibrated and demonstrated to recover the aromatic fraction of the IRAC 8 micron flux with a standard deviation of 6% for a training set of 40 SINGS galaxies (ranging from stellar to dust dominated) with both suitable mid-infrared Spitzer IRS spectra and equivalent photometry. A potential factor of two improvement could be realized with suitable 5.5 and 10 micron photometry, such as what may be provided in the future by JWST. The resulting technique is then applied to mid-infrared photometry for the 258 galaxies from the Local Volume Legacy (LVL) survey, a large sample dominated in number by low-luminosity dwarf galaxies for which obtaining comparable mid-infrared spectroscopy is not feasible. We find the total LVL luminosity due to five strong aromatic features in the 8 micron complex to be 2.47E10 solar luminosities with a mean volume density of 8.8E6 solar luminosities per cubic Megaparsec. Twenty-four of the LVL galaxies, corresponding to a luminosity cut at M = -18.22 in the B band, account for 90% of the aromatic luminosity. Using oxygen abundances compiled from the literature for 129 of the 258 LVL galaxies, we find a correlation between metallicity and the aromatic to total infrared emission ratio but not the aromatic to total 8 micron dust emission ratio. A possible explanation is that metallicity plays a role in the abundance of aromatic molecules relative to the total dust content, but other factors such as star formation and/or the local radiation field affect the excitation of those molecules.Comment: ApJ in press; 29 pages, 14 figures, 3 tables; emulateapj forma

β-Elemene Piperazine Derivatives Induce Apoptosis in Human Leukemia Cells through Downregulation of c-FLIP and Generation of ROS

β-Elemene is an active component of the herb medicine Curcuma Wenyujin with reported antitumor activity. To improve its antitumor ability, five novel piperazine derivatives of β-elemene, 13-(3-methyl-1-piperazinyl)-β-elemene (DX1), 13-(cis-3,5-dimethyl-1-piperazinyl)-β-elemene (DX2), 13-(4-ethyl-1-piperazinyl)-β-elemene (DX3), 13-(4-isopropyl-1-piperazinyl)-β-elemene (DX4) and 13-piperazinyl-β-elemene (DX5), were synthesized. The antiproliferative and apoptotic effects of these derivatives were determined in human leukemia HL-60, NB4, K562 and HP100-1 cells. DX1, DX2 and DX5, which contain a secondary amino moiety, were more active in inhibiting cell growth and in inducing apoptosis than DX3 and DX4. The apoptosis induction ability of DX1 was associated with the generation of hydrogen peroxide (H2O2), a decrease of mitochondrial membrane potential (MMP), and the activation of caspase-8. Pretreatment with the antioxidants N-acetylcysteine and catalase completely blocked DX1-induced H2O2 production, but only partially its activation of caspase-8 and induction of apoptosis. HL-60 cells were more sensitive than its H2O2-resistant subclone HP100-1 cells to DX1-induced apoptosis. The activation of caspase-8 by these compounds was correlated with the decrease in the levels of cellular FLICE-inhibitory protein (c-FLIP). The proteasome inhibitor MG-132 augmented the decrease in c-FLIP levels and apoptosis induced by these derivatives. FADD- and caspase-8-deficient Jurkat subclones have a decreased response to DX1-induced apoptosis. Our data indicate that these novel β-elemene piperazine derivatives induce apoptosis through the decrease in c-FLIP levels and the production of H2O2 which leads to activation of both death receptor- and mitochondrial-mediated apoptotic pathways

Vital function of PRELI and essential requirement of its LEA motif

Proteins containing the late embryogenesis abundant (LEA) motif comprise a conserved family, postulated to act as cell protectors. However, their function and mechanisms of action remain unclear. Here we show that PRELI, a mammalian LEA-containing homolog of yeast Ups1p, can associate with dynamin-like GTPase Optic Atrophy-1 (OPA1) and contribute to the maintenance of mitochondrial morphology. Accordingly, PRELI can uphold mitochondrial membrane potential (ΔΨm) and enhance respiratory chain (RC) function, shown by its capacity to induce complex-I/NADH dehydrogenase and ATP synthase expression, increase oxygen consumption and reduce reactive oxygen species (ROS) production. PRELI can also inhibit cell death induced by STS, TNF-α or UV irradiation. Moreover, in vitro and in vivo dominant-negative overexpression of mutant PRELI/LEA− (lacking the LEA motif) and transient in vitro PRELI-specific knockdown can render lymphocytes vulnerable to apoptosis, cause mouse embryo lethality and revert the resistance of lymphoma cells to induced death. Collectively, these data support the long-presumed notion of LEA protein-dependent mechanisms of cytoprotection and suggest that PRELI interacts with OPA1 to maintain mitochondria structures intact, sustain balanced ion−/proton+ gradients, promote oxidative phosphorylation reactions, regulate pro- and antiapoptotic protein traffic and enable cell responses to induced death. These findings may help to understand how bioenergetics is mechanistically connected with cell survival cues

ISM Properties in Low-Metallicity Environments III. The Dust Spectral Energy Distributions of II Zw 40, He 2-10 and NGC 1140

We present new 450 and 850 micron SCUBA data and 1.3 mm MAMBO data of the dwarf galaxies II Zw 40, He 2-10 and NGC 1140. Additional ISOCAM, IRAS as well as ground based data are used to construct the observed mid-infrared to millimeter spectral energy distribution of these galaxies. These spectral energy distributions are modeled in a self-consistent way, as was achieved with NGC 1569 (Galliano et al., 2003), synthesizing both the global stellar radiation field and the dust emission, with further constraints provided by the photoionisation of the gas. Our study shows that low-metallicity galaxies have very different dust properties compared to the Galaxy. Our main results are: (i) a paucity of PAHs which are likely destroyed by the hard penetrating radiation field, (ii) a very small (3-4 nm) average size of grains, consistent with the fragmentation and erosion of dust particles by the numerous shocks, (iii) a significant millimetre excess in the dust spectral energy distribution which can be explained by the presence of ubiquitous very cold dust (T=5-9 K) accounting for 40 to 80 % of the total dust mass, probably distributed in small clumps. We derive a range of gas-to-dust mass ratios between 300 and 2000, larger than the Galactic values and dust-to-metals ratios of 1/30 to 1/2. The modeled dust size distributions are used to synthesize an extinction curve for each galaxy. The UV slopes of the extinction curves resemble that observed in some regions in the Large Magellanic Cloud. The 2175 angstrom bumps of the modeled extinction curves are weaker than that of the Galaxy, except in the case of II Zw 40, where we are unable to accurately constrain the 2175 angstrom bump carrier.Comment: A&A, 19 pages, 31 figure

Quantum transport and utilization of free energy in protein α\alpha-helices

The essential biological processes that sustain life are catalyzed by protein nano-engines, which maintain living systems in far-from-equilibrium ordered states. To investigate energetic processes in proteins, we have analyzed the system of generalized Davydov equations that govern the quantum dynamics of multiple amide I exciton quanta propagating along the hydrogen-bonded peptide groups in $\alpha$-helices. Computational simulations have confirmed the generation of moving Davydov solitons by applied pulses of amide I energy for protein $\alpha$-helices of varying length. The stability and mobility of these solitons depended on the uniformity of dipole-dipole coupling between amide I oscillators, and the isotropy of the exciton-phonon interaction. Davydov solitons were also able to quantum tunnel through massive barriers, or to quantum interfere at collision sites. The results presented here support a nontrivial role of quantum effects in biological systems that lies beyond the mechanistic support of covalent bonds as binding agents of macromolecular structures. Quantum tunneling and interference of Davydov solitons provide catalytically active macromolecular protein complexes with a physical mechanism allowing highly efficient transport, delivery, and utilization of free energy, besides the evolutionary mandate of biological order that supports the existence of such genuine quantum phenomena, and may indeed demarcate the quantum boundaries of life.Comment: 40 pages, 20 figure

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data