183 research outputs found
Brown Dwarfs in the Pleiades Cluster Confirmed by the Lithium Test
We present 10 m Keck spectra of the two Pleiades brown dwarfs Teide 1 and
Calar 3 showing a clear detection of the 670.8 nm Li resonance line. In Teide
1, we have also obtained evidence for the presence of the subordinate line at
812.6 nm. A high Li abundance (log N(Li) >= 2.5), consistent with little if any
depletion, is inferred from the observed lines. Since Pleiades brown dwarfs are
unable to burn Li the significant preservation of this fragile element confirms
the substellar nature of our two objects. Regardless of their age, their low
luminosities and Li content place Teide 1 and Calar 3 comfortably in the
genuine brown dwarf realm. Given the probable age of the Pleiades cluster,
their masses are estimated at 55 +- 15 Jupiter masses.Comment: 14 pages gzipped and uuencoded. Figures are included. Also available
at http://www.iac.es/. Accepted for publication in ApJ Letter
Rotational modulation of the photospheric and chromospheric activity in the young, single K2-dwarf PW And
High resolution echelle spectra of PW And (HD~1405) have been taken during
eight observing runs from 1999 to 2002. The detailed analysis of the spectra
allow us to determine its spectral type (K2V), mean heliocentric radial
velocity (V_hel = -11.15 km/s) rotational velocity (vsin{i} = 22.6 km/s), and
equivalent width of the lithium line 6707.8 AA (EW(LiI) = 273 mAA). The
kinematic (Galactic Velocity (U, V, W)) confirms its membership of the Local
Association moving group, in agreement with the age (30 to 80 Myrs) inferred
from the color magnitude diagram and the lithium equivalent width. Photospheric
activity (presence of cool spots that disturb the profiles of the photospheric
lines) has been detected as changes in the the bisectors of the cross
correlation function (CCF) resulting of cross-correlate the spectra of PW And
with the spectrum of a non active star of similar spectral type. These
variations of the CCF bisectors are related to the variations in the measured
radial velocities and are modulated with a period similar to the photometric
period of the star. At the same time, chromospheric activity has been analyzed,
using the spectral subtraction technique and simultaneous spectroscopic
observations of the H_alpha, H_beta, NaI D_1 and D_2$, HeI D_3, MgI b triplet,
CaII H&K, and CaII infrared triplet lines. A flare was observed during the last
observing run of 2001, showing an enhancement in the observed chromospheric
lines. A less powerful flare was observed on 2002 August 23. The variations of
the chromospheric activity indicators seem to be related to the photospheric
activity. A correlation between radial velocity, changes in the CCF bisectors
and equivalent width of different chromospheric lines is observed with a
different behaviour between epochs 1999, 2001 and 2002.Comment: Latex file with 20 pages, 21 figures tar'ed gzip'ed. Full postscript
(text, figures and tables) available at
http://www.ucm.es/info/Astrof/users/dmg/pub_dmg.html Accepted for publication
in: Astronomy & Astrophysics (A&A
X-rays in the Orion Nebula Cluster: Constraints on the origins of magnetic activity in pre-main sequence stars
A recent Chandra/ACIS observation of the Orion Nebula Cluster detected 1075
sources (Feigelson et al. 2002), providing a uniquely large and well-defined
sample to study the dependence of magnetic activity on bulk properties for
stars descending the Hayashi tracks. The following results are obtained: (1)
X-ray luminosities L_t in the 0.5-8 keV band are strongly correlated with
bolometric luminosity with = -3.8 for stars with masses 0.7<M<2
Mo, an order of magnitude below the main sequence saturation level; (2) the
X-ray emission drops rapidly below this level in some or all stars with 2<M<3
Mo; (3) the presence or absence of infrared circumstellar disks has no apparent
relation to X-ray levels; and (4) X-ray luminosities exhibit a slight rise as
rotational periods increase from 0.4 to 20 days. This last finding stands in
dramatic contrast to the strong anticorrelation between X-rays and period seen
in main sequence stars.
The absence of a strong X-ray/rotation relationship in PMS stars, and
particularly the high X-ray values seen in some very slowly rotating stars, is
a clear indication that the mechanisms of magnetic field generation differ from
those operating in main sequence stars. The most promising possibility is a
turbulent dynamo distributed throughout the deep convection zone, but other
models such as alpha-Omega dynamo with `supersaturation' or relic core fields
are not immediately excluded. The drop in magnetic activity in
intermediate-mass stars may reflect the presence of a significant radiative
core. The evidence does not support X-ray production in large-scale star-disk
magnetic fields.Comment: 51 pages, 8 figures. To appear in the Astrophysical Journa
New Insights into White-Light Flare Emission from Radiative-Hydrodynamic Modeling of a Chromospheric Condensation
(abridged) The heating mechanism at high densities during M dwarf flares is
poorly understood. Spectra of M dwarf flares in the optical and
near-ultraviolet wavelength regimes have revealed three continuum components
during the impulsive phase: 1) an energetically dominant blackbody component
with a color temperature of T 10,000 K in the blue-optical, 2) a smaller
amount of Balmer continuum emission in the near-ultraviolet at lambda 3646
Angstroms and 3) an apparent pseudo-continuum of blended high-order Balmer
lines. These properties are not reproduced by models that employ a typical
"solar-type" flare heating level in nonthermal electrons, and therefore our
understanding of these spectra is limited to a phenomenological interpretation.
We present a new 1D radiative-hydrodynamic model of an M dwarf flare from
precipitating nonthermal electrons with a large energy flux of erg
cm s. The simulation produces bright continuum emission from a
dense, hot chromospheric condensation. For the first time, the observed color
temperature and Balmer jump ratio are produced self-consistently in a
radiative-hydrodynamic flare model. We find that a T 10,000 K
blackbody-like continuum component and a small Balmer jump ratio result from
optically thick Balmer and Paschen recombination radiation, and thus the
properties of the flux spectrum are caused by blue light escaping over a larger
physical depth range compared to red and near-ultraviolet light. To model the
near-ultraviolet pseudo-continuum previously attributed to overlapping Balmer
lines, we include the extra Balmer continuum opacity from Landau-Zener
transitions that result from merged, high order energy levels of hydrogen in a
dense, partially ionized atmosphere. This reveals a new diagnostic of ambient
charge density in the densest regions of the atmosphere that are heated during
dMe and solar flares.Comment: 50 pages, 2 tables, 13 figures. Accepted for publication in the Solar
Physics Topical Issue, "Solar and Stellar Flares". Version 2 (June 22, 2015):
updated to include comments by Guest Editor. The final publication is
available at Springer via http://dx.doi.org/10.1007/s11207-015-0708-
Lithium and Halpha in stars and brown dwarfs of sigma Orionis
We present intermediate- and low-resolution optical spectra around Halpha and
LiI 6708 A for a sample of 25 low mass stars and 2 brown dwarfs with confirmed
membership in the pre-main sequence stellar sigma Orionis cluster. Our
observations are intended to investigate the age of the cluster. The spectral
types derived for our target sample are found to be in the range K6-M8.5, which
corresponds to a mass interval of roughly 1.2-0.02 Msun on the basis of
state-of-the-art evolutionary models. Radial velocities (except for one object)
are found to be consistent with membership in the Orion complex. All cluster
members show considerable Halpha emission and LiI in absorption, which is
typical of very young ages. We find that our pseudo-equivalent widths appear
rather dispersed (and intense in the case of Halpha) for objects cooler than
M3.5 spectral class, occurring at the approximate mass where low mass stars are
expected to become fully convective. The least massive brown dwarf in our
sample, SOri 45 (M8.5, ~0.02 Msun), displays variable Halpha emission and a
radial velocity that differs from the cluster mean velocity. Tentative
detection of forbidden lines in emission indicates that this brown dwarf may be
accreting mass from a surrounding disk. We also present recent computations of
LiI curves of growth for low gravities and for the temperature interval (about
4000-2600 K) of our sample. The comparison of our observations to these
computations allows us to infer that no lithium depletion has yet taken place
in sigma Orionis, and that the observed pseudo-equivalent widths are consistent
with a cluster initial lithium abundance close to the cosmic value. Hence, the
upper limit to the sigma Orionis cluster age can be set at 8 Myr, with a most
likely value around 2-4 Myr.Comment: 17 pages (figures included). Accepted for publication in A&
Transgene Expression Is Associated with Copy Number and Cytomegalovirus Promoter Methylation in Transgenic Pigs
Transgenic animals have been used for years to study gene function, produce important proteins, and generate models for the study of human diseases. However, inheritance and expression instability of the transgene in transgenic animals is a major limitation. Copy number and promoter methylation are known to regulate gene expression, but no report has systematically examined their effect on transgene expression. In the study, we generated two transgenic pigs by somatic cell nuclear transfer (SCNT) that express green fluorescent protein (GFP) driven by cytomegalovirus (CMV). Absolute quantitative real-time PCR and bisulfite sequencing were performed to determine transgene copy number and promoter methylation level. The correlation of transgene expression with copy number and promoter methylation was analyzed in individual development, fibroblast cells, various tissues, and offspring of the transgenic pigs. Our results demonstrate that transgene expression is associated with copy number and CMV promoter methylation in transgenic pigs
Systematic Reviews of Animal Experiments Demonstrate Poor Human Clinical and Toxicological Utility
The assumption that animal models are reasonably predictive of human outcomes provides the basis for their widespread use in toxicity testing and in biomedical research aimed at developing cures for human diseases. To investigate the validity of this assumption, the comprehensive Scopus biomedical bibliographic databases were searched for published systematic reviews of the human clinical or toxicological utility of animal experiments. In 20 reviews in which clinical utility was examined, the authors concluded that animal models were either significantly useful in contributing to the development of clinical interventions, or were substantially consistent with clinical outcomes, in only two cases, one of which was contentious. These included reviews of the clinical utility of experiments expected by ethics committees to lead to medical advances, of highly-cited experiments published in major journals, and of chimpanzee experiments — those involving the species considered most likely to be predictive of human outcomes. Seven additional reviews failed to clearly demonstrate utility in predicting human toxicological outcomes, such as carcinogenicity and teratogenicity. Consequently, animal data may not generally be assumed to be substantially useful for these purposes. Possible causes include interspecies differences, the distortion of outcomes arising from experimental environments and protocols, and the poor methodological quality of many animal experiments, which was evident in at least 11 reviews. No reviews existed in which the majority of animal experiments were of good methodological quality. Whilst the effects of some of these problems might be minimised with concerted effort (given their widespread prevalence), the limitations resulting from interspecies differences are likely to be technically and theoretically impossible to overcome. Non-animal models are generally required to pass formal scientific validation prior to their regulatory acceptance. In contrast, animal models are simply assumed to be predictive of human outcomes. These results demonstrate the invalidity of such assumptions. The consistent application of formal validation studies to all test models is clearly warranted, regardless of their animal, non-animal, historical, contemporary or possible future status. Likely benefits would include, the greater selection of models truly predictive of human outcomes, increased safety of people exposed to chemicals that have passed toxicity tests, increased efficiency during the development of human pharmaceuticals and other therapeutic interventions, and decreased wastage of animal, personnel and financial resources. The poor human clinical and toxicological utility of most animal models for which data exists, in conjunction with their generally substantial animal welfare and economic costs, justify a ban on animal models lacking scientific data clearly establishing their human predictivity or utility
Comparison of Internal Ribosome Entry Site (IRES) and Furin-2A (F2A) for Monoclonal Antibody Expression Level and Quality in CHO Cells
10.1371/journal.pone.0063247PLoS ONE85
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