205 research outputs found
RNA Interference Is Responsible for Reduction of Transgene Expression after Sleeping Beauty Transposase Mediated Somatic Integration
Integrating non-viral vectors based on transposable elements are widely used for genetically engineering mammalian cells in functional genomics and therapeutic gene transfer. For the Sleeping Beauty (SB) transposase system it was demonstrated that convergent transcription driven by the SB transposase inverted repeats (IRs) in eukaryotic cells occurs after somatic integration. This could lead to formation of double-stranded RNAs potentially presenting targets for the RNA interference (RNAi) machinery and subsequently resulting into silencing of the transgene. Therefore, we aimed at investigating transgene expression upon transposition under RNA interference knockdown conditions.
To establish RNAi knockdown cell lines we took advantage of the P19 protein, which is derived from the tomato bushy stunt virus. P19 binds and inhibits 21 nucleotides long, small-interfering RNAs and was shown to sufficiently suppress RNAi. We found that transgene expression upon SB mediated transposition was enhanced, resulting into a 3.2-fold increased amount of colony forming units (CFU) after transposition. In contrast, if the transgene cassette is insulated from the influence of chromosomal position effects by the chicken-derived cHS4 insulating sequences or when applying the Forg Prince transposon system, that displays only negligible transcriptional activity, similar numbers of CFUs were obtained.
In summary, we provide evidence for the first time that after somatic integration transposon derived transgene expression is regulated by the endogenous RNAi machinery. In the future this finding will help to further improve the molecular design of the SB transposase vector system
Plant growth retardation and conserved miRNAs are correlated to hibiscus chlorotic ringspot virus infection
10.1371/journal.pone.0085476PLoS ONE812-POLN
Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance
Crown gall disease of grapevine induced by Agrobacterium vitis or Agrobacterium tumefaciens causes serious
economic losses in viticulture. To establish crown gall-resistant lines, somatic proembryos of Vitis
berlandieri × V. rupestris cv. 'Richter 110' rootstock were transformed with an oncogene-silencing transgene
based on iaaM and ipt oncogene sequences from octopine-type, tumor-inducing (Ti) plasmid pTiA6. Twentyone
transgenic lines were selected, and their transgenic nature was confirmed by polymerase chain reaction
(PCR). These lines were inoculated with two A. tumefaciens and three A. vitis strains. Eight lines showed
resistance to octopine-type A. tumefaciens A348. Resistance correlated with the expression of the silencing
genes. However, oncogene silencing was mostly sequence specific because these lines did not abolish
tumorigenesis by A. vitis strains or nopaline-type A. tumefaciens C58
Plasma miRNA as Biomarkers for Assessment of Total-Body Radiation Exposure Dosimetry
The risk of radiation exposure, due to accidental or malicious release of ionizing radiation, is a major public health concern. Biomarkers that can rapidly identify severely-irradiated individuals requiring prompt medical treatment in mass-casualty incidents are urgently needed. Stable blood or plasma-based biomarkers are attractive because of the ease for sample collection. We tested the hypothesis that plasma miRNA expression profiles can accurately reflect prior radiation exposure. We demonstrated using a murine model that plasma miRNA expression signatures could distinguish mice that received total body irradiation doses of 0.5 Gy, 2 Gy, and 10 Gy (at 6 h or 24 h post radiation) with accuracy, sensitivity, and specificity of above 90%. Taken together, these data demonstrate that plasma miRNA profiles can be highly predictive of different levels of radiation exposure. Thus, plasma-based biomarkers can be used to assess radiation exposure after mass-casualty incidents, and it may provide a valuable tool in developing and implementing effective countermeasures
Compact groups with a dense free abelian subgroup
The compact groups having a dense infinite cyclic subgroup (known as monothetic compact groups) have been studied by many authors for their relevance and nice applications. In this paper we describe in full details the compact groups with a dense free abelian subgroup and we describe the minimum rank of such a subgroup of . Surprisingly, it is either finite or coincides with the density character of .
Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes
Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration
Mycorrhiza-induced resistance: more than the sum of its parts?
Plants can develop an enhanced defensive capacity in response to infection by arbuscular mycorrhizal fungi (AMF). This ‘mycorrhiza-induced resistance’ (MIR) provides systemic protection against a wide range of attackers and shares characteristics with systemic acquired resistance (SAR) after pathogen infection and induced systemic resistance (ISR) following root colonisation by non-pathogenic rhizobacteria. It is commonly assumed that fungal stimulation of the plant immune system is solely responsible for MIR. In this opinion article, we present a novel model of MIR that integrates different aspects of the induced resistance phenomenon. We propose that MIR is a cumulative effect of direct plant responses to mycorrhizal infection and indirect immune responses to ISR-eliciting rhizobacteria in the mycorrhizosphere
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