74 research outputs found
Elimination of Hepatitis C Virus from Hepatocytes by a Selective Activation of Therapeutic Molecules
To eliminate hepatitis C virus (HCV) from infected hepatocytes, we generated two therapeutic molecules specifically activated in cells infected with HCV. A dominant active mutant of interferon (IFN) regulatory factor 7 (IRF7) and a negative regulator of HCV replication, VAP-C (Vesicle-associated membrane protein-associated protein subtype C), were fused with the C-terminal region of IPS-1 (IFNÎČ promoter stimulator-1), which includes an HCV protease cleavage site that was modified to be localized on the ER membrane, and designated cIRF7 and cVAP-C, respectively. In cells expressing the HCV protease, cIRF7 was cleaved and the processed fragment was migrated into the nucleus, where it activated various IFN promoters, including promoters of IFNα6, IFNÎČ, and IFN stimulated response element. Activation of the IFN promoters and suppression of viral RNA replication were observed in the HCV replicon cells and in cells infected with the JFH1 strain of HCV (HCVcc) by expression of cIRF7. Suppression of viral RNA replication was observed even in the IFN-resistant replicon cells by the expression of cIRF7. Expression of the cVAP-C also resulted in suppression of HCV replication in both the replicon and HCVcc infected cells. These results suggest that delivery of the therapeutic molecules into the liver of hepatitis C patients, followed by selective activation of the molecules in HCV-infected hepatocytes, is a feasible method for eliminating HCV
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ć ±æäŸăŻïŒç€ŸäŒæŽ»ćăă§ăăć Žă§èĄăăăšăæçă§ăăăăšăç€șćăăă.The purpose of this study was to countermeasure to living alone elderly when disaster occurs. A total of 258 elderly residents participates. A questionnaire included the following scales and items: Disaster shelter. Recognition a formal consultation. Take advantage of a formal consultation. Take advantage number of adviser. Recognition a countermeasure. In life a pleasure. Emotional support. Self Esteem scale. The more elderly, living alone elderly than living with family elderly. The living alone elderly did not know that Fukuoka-city hall and town office is daily living consultation. The urban area to progress in disaster countermeasures, attention to live-alone elderly, whose are provided emotional support, have in life a pleasure, have a place of social activities.ćèè«æ = Original articl
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ObjectiveïŒThis study aimed to clarify the relationship between sleep and subjective health condition by focusing on disaster-affected workers six months after the Kumamoto earthquake.MethodsïŒThe study population comprised 676 workers at small and medium-sized companies located in Kamimashikigun, Kumamoto Prefecture, where damage after the Kumamoto earthquake was severe. Participants were sent a questionnaire with items related to basic characteristics ïŒsex, age, and family membersïŒ, damage to home after the earthquake, subjective symptoms after the earthquake, injury from the earthquakes, changes in income, subjective wellbeing, changes in workload after the earthquake, Athens Insomnia ScaleïŒAISïŒ, Impact of Event Scale-RevisedïŒIES-RïŒ, General Health QuestionnaireïŒGHQ 28ïŒ, and health related QOL Short-Form Health SurveyïŒSF-8ïŒ. Responses were collected from 456 people at 13 companiesïŒ68.1% response rateïŒ. Of these, 438 responsesïŒ96.1%ïŒ were valid. This study was approved by the ethical review committee of Fukuoka University School of Medicine.ResultsïŒThe participants included 334 males and 104 females with an average age of 43.3 years. Two groups were created based on Abbreviated Injury ScaleïŒAISïŒscoresïŒ279 participants were in the insomnia group and 159 participants were in the non-insomnia group. Compared with that in the non-insomnia group, the patients in the insomnia group were old, had poor subjective health status, and were more likely to have subjective symptoms and injury after the earthquake. The study found a significant positive correlation with intrusion and hyperarousal symptoms in the IES-R subscales. In the SF-8 responses, there were significant negative correlations with BPïŒBodily PainïŒ, GHïŒGeneral HealthïŒ, VTïŒVitalityïŒ, SFïŒSocial FunctioningïŒ, REïŒRole EmotionalïŒ, MHïŒMental HealthïŒ, and MCSïŒMental Component SummaryïŒ. In a logistic regression analysis of sleeping condition in Athens Insomnia ScaleïŒAISïŒ, BPORïŒ 0.942, CIïŒ 0.907-0.978and avoidance symptomsORïŒ0.881, CIïŒ0.799-0.971improved sleeping condition, whereas hyperarousal symptomsORïŒ1.553, CIïŒ1.324-1.823, social life functionORïŒ0.954, CIïŒ0.916-0.994, and daily role functionïŒmentalïŒïŒ»ORïŒ0.916, CIïŒ0.866-0.97were negatively related with good sleeping condition.DiscussionïŒThe Kumamoto earthquake influenced the sleep quality for most disaster-affected workers, with approximately 60% reporting insomnia. Because workers with insomnia may exhibit hidden subjective symptoms or suffer from mental stress, long-term preventive systems should be developed and established by companies to improve worker health. Longitudinal studies are required to further examine factors related to sleep condition and outcomes of support systems.ćèè«æ = Original articl
Completion of Hepatitis C Virus Replication Cycle in Heterokaryons Excludes Dominant Restrictions in Human Non-liver and Mouse Liver Cell Lines
Hepatitis C virus (HCV) is hepatotropic and only infects humans and chimpanzees. Consequently, an immunocompetent small animal model is lacking. The restricted tropism of HCV likely reflects specific host factor requirements. We investigated if dominant restriction factors expressed in non-liver or non-human cell lines inhibit HCV propagation thus rendering these cells non-permissive. To this end we explored if HCV completes its replication cycle in heterokaryons between human liver cell lines and non-permissive cell lines from human non-liver or mouse liver origin. Despite functional viral pattern recognition pathways and responsiveness to interferon, virus production was observed in all fused cells and was only ablated when cells were treated with exogenous interferon. These results exclude that constitutive or virus-induced expression of dominant restriction factors prevents propagation of HCV in these cell types, which has important implications for HCV tissue and species tropism. In turn, these data strongly advocate transgenic approaches of crucial human HCV cofactors to establish an immunocompetent small animal model
Open Data from the Third Observing Run of LIGO, Virgo, KAGRA, and GEO
The global network of gravitational-wave observatories now includes five detectors, namely LIGO Hanford, LIGO Livingston, Virgo, KAGRA, and GEO 600. These detectors collected data during their third observing run, O3, composed of three phases: O3a starting in 2019 April and lasting six months, O3b starting in 2019 November and lasting five months, and O3GK starting in 2020 April and lasting two weeks. In this paper we describe these data and various other science products that can be freely accessed through the Gravitational Wave Open Science Center at https://gwosc.org. The main data set, consisting of the gravitational-wave strain time series that contains the astrophysical signals, is released together with supporting data useful for their analysis and documentation, tutorials, as well as analysis software packages
Open data from the third observing run of LIGO, Virgo, KAGRA and GEO
The global network of gravitational-wave observatories now includes five
detectors, namely LIGO Hanford, LIGO Livingston, Virgo, KAGRA, and GEO 600.
These detectors collected data during their third observing run, O3, composed
of three phases: O3a starting in April of 2019 and lasting six months, O3b
starting in November of 2019 and lasting five months, and O3GK starting in
April of 2020 and lasting 2 weeks. In this paper we describe these data and
various other science products that can be freely accessed through the
Gravitational Wave Open Science Center at https://gwosc.org. The main dataset,
consisting of the gravitational-wave strain time series that contains the
astrophysical signals, is released together with supporting data useful for
their analysis and documentation, tutorials, as well as analysis software
packages.Comment: 27 pages, 3 figure
Open data from the third observing run of LIGO, Virgo, KAGRA, and GEO
The global network of gravitational-wave observatories now includes five detectors, namely LIGO Hanford, LIGO Livingston, Virgo, KAGRA, and GEO 600. These detectors collected data during their third observing run, O3, composed of three phases: O3a starting in 2019 April and lasting six months, O3b starting in 2019 November and lasting five months, and O3GK starting in 2020 April and lasting two weeks. In this paper we describe these data and various other science products that can be freely accessed through the Gravitational Wave Open Science Center at https://gwosc.org. The main data set, consisting of the gravitational-wave strain time series that contains the astrophysical signals, is released together with supporting data useful for their analysis and documentation, tutorials, as well as analysis software packages
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