Minimum and Maximum Limit to Number of Myosin II Motors Participating in an Ensemble Motility

Extensive research on centrifugal compressors has been planned to define diffuser stall limits for a group of stages characterized by low blade-outlet-width-to-impeller-radius-ratio. Very little data is available on this centrifugal compressor family, especially for the last stage configuration. In addition, the most important stall diffuser prediction criteria barely cover this machine type. Many experimental tests have been planned to investigate several geometry variations. A simulated stage with a backward channel upstream, a 2D impeller with a vaneless diffuser and a constant cross section volute downstream constitute the basic geometry. Several diffuser geometries with different widths, pinch shapes, diffusion ratios were tested. Test results and conclusions are shown in the paper in terms of critical diffuser inlet flow angles, flow coefficients at stall inception and stage working ranges. The main task of the present work is to increase the knowledge and the amount of available data to characterize rotating stall phenomena, in particular for very narrow stages

Bayesian Network Analysis of Lysine Biosynthesis Pathway in Rice

Lysine is the first limiting essential amino acid in rice because it is present in the lowest quantity compared to all the other amino acids. Amino acids are the building block of proteins and play an essential role in maintaining the human body’s healthy functioning. Rice is a staple food for more than half of the global population; thus, increasing the lysine content in rice will help improve global health. In this paper, we studied the lysine biosynthesis pathway in rice (Oryza sativa) to identify the regulators of the lysine reporter gene LYSA (LOC_Os02g24354). Genetically intervening at the regulators has the potential to increase the overall lysine content in rice. We modeled the lysine biosynthesis pathway in rice seedlings under normal and saline (NaCl) stress conditions using Bayesian networks. We estimated the model parameters using experimental data and identified the gene DAPF(LOC_Os12g37960) as a positive regulator of the lysine reporter gene LYSA under both normal and saline stress conditions. Based on this analysis, we conclude that the gene DAPF is a potent candidate for genetic intervention. Upregulating DAPF using methods such as CRISPR-Cas9 gene editing strategy has the potential to upregulate the lysine reporter gene LYSA and increase the overall lysine content in rice