Ten millennia of hepatitis B virus evolution

Hepatitis B virus (HBV) has been infecting humans for millennia and remains a global health problem, but its past diversity and dispersal routes are largely unknown. We generated HBV genomic data from 137 Eurasians and Native Americans dated between ~10,500 and ~400 years ago. We date the most recent common ancestor of all HBV lineages to between ~20,000 and 12,000 years ago, with the virus present in European and South American hunter-gatherers during the early Holocene. After the European Neolithic transition, Mesolithic HBV strains were replaced by a lineage likely disseminated by early farmers that prevailed throughout western Eurasia for ~4000 years, declining around the end of the 2nd millennium BCE. The only remnant of this prehistoric HBV diversity is the rare genotype G, which appears to have reemerged during the HIV pandemic

The population history of northeastern Siberia since the Pleistocene.

Northeastern Siberia has been inhabited by humans for more than 40,000 years but its deep population history remains poorly understood. Here we investigate the late Pleistocene population history of northeastern Siberia through analyses of 34 newly recovered ancient genomes that date to between 31,000 and 600 years ago. We document complex population dynamics during this period, including at least three major migration events: an initial peopling by a previously unknown Palaeolithic population of 'Ancient North Siberians' who are distantly related to early West Eurasian hunter-gatherers; the arrival of East Asian-related peoples, which gave rise to 'Ancient Palaeo-Siberians' who are closely related to contemporary communities from far-northeastern Siberia (such as the Koryaks), as well as Native Americans; and a Holocene migration of other East Asian-related peoples, who we name 'Neo-Siberians', and from whom many contemporary Siberians are descended. Each of these population expansions largely replaced the earlier inhabitants, and ultimately generated the mosaic genetic make-up of contemporary peoples who inhabit a vast area across northern Eurasia and the Americas

Ten millennia of hepatitis B virus evolution

Hepatitis B virus (HBV) has been infecting humans for millennia and remains a global health problem, but its past diversity and dispersal routes are largely unknown. We generated HBV genomic data from 137 Eurasians and Native Americans dated between similar to 10,500 and similar to 400 years ago. We date the most recent common ancestor of all HBV lineages to between similar to 20,000 and 12,000 years ago, with the virus present in European and South American hunter-gatherers during the early Holocene. After the European Neolithic transition, Mesolithic HBV strains were replaced by a lineage likely disseminated by early farmers that prevailed throughout western Eurasia for similar to 4000 years, declining around the end of the 2nd millennium BCE. The only remnant of this prehistoric HBV diversity is the rare genotype G, which appears to have reemerged during the HIV pandemic.Molecular Technology and Informatics for Personalised Medicine and Healt

Ancient DNA reveals prehistoric gene-flow from Siberia in the complex human population history of north east Europe

North East Europe harbors a high diversity of cultures and languages, suggesting a complex genetic history. Archaeological, anthropological, and genetic research has revealed a series of influences from Western and Eastern Eurasia in the past. While genetic data from modern-day populations is commonly used to make inferences about their origins and past migrations, ancient DNA provides a powerful test of such hypotheses by giving a snapshot of the past genetic diversity. In order to better understand the dynamics that have shaped the gene pool of North East Europeans, we generated and analyzed 34 mitochondrial genotypes from the skeletal remains of three archaeological sites in northwest Russia. These sites were dated to the Mesolithic and the Early Metal Age (7,500 and 3,500 uncalibrated years Before Present). We applied a suite of population genetic analyses (principal component analysis, genetic distance mapping, haplotype sharing analyses) and compared past demographic models through coalescent simulations using Bayesian Serial SimCoal and Approximate Bayesian Computation. Comparisons of genetic data from ancient and modern-day populations revealed significant changes in the mitochondrial makeup of North East Europeans through time. Mesolithic foragers showed high frequencies and diversity of haplogroups U (U2e, U4, U5a), a pattern observed previously in European hunter-gatherers from Iberia to Scandinavia. In contrast, the presence of mitochondrial DNA haplogroups C, D, and Z in Early Metal Age individuals suggested discontinuity with Mesolithic hunter-gatherers and genetic influx from central/eastern Siberia. We identified remarkable genetic dissimilarities between prehistoric and modern-day North East Europeans/Saami, which suggests an important role of post-Mesolithic migrations from Western Europe and subsequent population replacement/extinctions. This work demonstrates how ancient DNA can improve our understanding of human population movements across Eurasia. It contributes to the description of the spatio-temporal distribution of mitochondrial diversity and will be of significance for future reconstructions of the history of Europeans.Clio Der Sarkissian, Oleg Balanovsky, Guido Brandt, Valery Khartanovich, Alexandra Buzhilova, Sergey Koshel, Valery Zaporozhchenko, Detlef Gronenborn, Vyacheslav Moiseyev, Eugen Kolpakov, Vladimir Shumkin, Kurt W. Alt, Elena Balanovska, Alan Cooper, Wolfgang Haak, the Genographic Consortiu

Ten millennia of hepatitis B virus evolution

Hepatitis B virus (HBV) has been infecting humans for millennia and remains a global health problem, but its past diversity and dispersal routes are largely unknown. We generated HBV genomic data from 137 Eurasians and Native Americans dated between ~10,500 and ~400 years ago. We date the most recent common ancestor of all HBV lineages to between ~20,000 and 12,000 years ago, with the virus present in European and South American hunter-gatherers during the early Holocene. After the European Neolithic transition, Mesolithic HBV strains were replaced by a lineage likely disseminated by early farmers that prevailed throughout western Eurasia for ~4000 years, declining around the end of the 2nd millennium BCE. The only remnant of this prehistoric HBV diversity is the rare genotype G, which appears to have reemerged during the HIV pandemic

ПРИМЕНЕНИЕ ЛЕВОСИМЕНДАНА В ПЕРИОПЕРАЦИОННОМ ПЕРИОДЕ У БОЛЬНЫХ С ОСТРЫМ КОРОНАРНЫМ СИНДРОМОМ ПРИ АОРТОКОРОНАРНОМ ШУНТИРОВАНИИ

Цель. Оценка эффективности инотропного эффекта левосимендана при выполнении аортокоронарного шунтирования (АКШ). Материалы и методы. У 85 пациентов по поводу острого коронарного синдрома (ОКС) выполнено АКШ. У 40 больных (1–я группа) использовали локальный протокол анестезии и интенсивной терапии, у 45 (2–я группа) – дополнительно вводили левосимендан. Определяли ударный и сердечный индекс (УИ, СИ), фракцию выброса (ФВ) левого желудочка (ЛЖ), уровень КФК–МВ, TnI, NT–proBNP, лактата, доставку, потребление и коэффициент экстракции кислорода, дозу добутамина. Результаты. После перевода пациентов в отделение интенсивной терапии (ОИТ) во 2–й группе достоверно увеличивались ФВЛЖ, УИ, СИ. Применение левосимендана позволяло поддерживать доставку кислорода на исходном уровне. Влияние левосимендана на уровень маркеров повреждения миокарда (МПМ) отмечали только на этапе перевода больных из ОИТ. Выводы. Применение левосимендана позволило уменьшить дозу добутамина, улучшить коронарное кровообращение на фоне оптимизации гемодинамики, улучшить кислородный бюджет, оказывает замедленное влияние на уровень МПМ

Integrated stable isotopic and radiocarbon analyses of Neolithic and bronze age hunter-gatherers from the Little Sea and Upper Lena micro- regions, Cis-Baikal, Siberia

The Lake Baikal region of southern Siberia has a rich mortuary record that has provided the most comprehensive isotopic database for palaeodietary studies of north-temperate hunter-gatherers in the world, permitting more detailed reconstructions and finer-grained research questions than are usually possible. Building on previous work, this study contributes new δ13C, δ15N, and AMS radiocarbon dating results from the cemeteries of Verkholensk (n = 44) in the Upper Lena River micro-region and Ulan-Khada (n = 19) in the Little Sea micro-region. Our results reveal that the Late Neolithic (LN, 5570–4600 cal BP) individuals at Verkholensk exhibit higher δ15N values than in the Early Bronze Age (EBA, 4600–3700 cal BP), suggesting a shift to a more terrestrial diet, possibly in response to climate-induced environmental changes. In addition, EBA individuals at Verkholensk differ in both δ13C and δ15N from those at the nearby site of Obkhoi, suggesting territorial divisions at a surprisingly small scale, although there is a diachronic component that needs to be considered, highlighting the need for additional work on freshwater reservoir corrections for the Upper Lena micro-region. The Ulan-Khada EBA results are consistent with the ‘Game-Fish’ and ‘Game-Fish-Seal’ dietary patterns previously identified in the Little Sea micro-region. The now substantial Little Sea micro-region EBA dataset—— allows for more subtle differences in diet to be identified, namely that EBA females with Game-Fish-Seal diets for the whole of the Little Sea sample display significantly lower mean δ13C values than their male counterparts, providing some of the first evidence for sex-based dietary distinctions in Lake Baikal. A small number of δ13C and/or δ15N outliers were identified at both Verkholensk and Ulan-Khada that may support previous suggestions of individual mobility between the Upper Lena and Little Sea micro-regions. Exploratory use of δ18O isotopes in bone collagen offers a novel line of support for this scenario, confirming a number of independently identified outliers.</p