Ecological Characteristics of Streams in the Barrenlands near Lac de Gras, N.W.T., Canada

We examined spatiotemporal variation in the physical, chemical, and biological characteristics of pristine streams that represent a range of conditions near Lac de Gras in the Barrenlands region of the Northwest Territories, Canada. Principal component analysis organized streams into four groups on the basis of seven physical characteristics. Despite broad differences among groups in physical characteristics, variation in chemical and biological characteristics was generally not large, with only pH and coarse particulate organic matter differing among the four groups. Nevertheless, several chemical and biological variables were correlated with physical characteristics, particularly measures of stream size (bankfull width and depth, drainage area, and stream discharge). Annual variability in climate affected stream temperature and discharge and influenced several biotic characteristics, particularly the growth of young-of-the-year arctic grayling (Thymallus arcticus). Barrenland streams share basic characteristics of Alaskan tundra streams, as a result of similar climatic regimes. Key differences between the two areas, however, appear related to the lake-outlet nature of the Barrenland streams, which may contribute to higher growth of young-of-the-year arctic grayling than would be expected from regional climate.On a étudié la variation spatio-temporelle dans les caractéristiques physiques, chimiques et biologiques de cours d'eau vierges qui témoignent d'une gamme de conditions près du lac de Gras dans la région des Barrenlands, dans les Territoires du Nord-Ouest, au Canada. L'analyse des composantes principales a divisé les cours d'eau en quatre groupes, d'après sept caractéristiques physiques. En dépit de différences marquées dans ces dernières entre les groupes, la variation dans les attributs chimiques et biologiques n'était généralement pas très grande, avec seulement le pH et les grosses particules organiques qui montraient des différences entre les quatre groupes. Plusieurs variables chimiques et biologiques étaient néanmoins corrélées avec les attributs physiques, en particulier les mesures de la taille des cours d'eau (largeur et profondeur du débordement, aire de drainage et débit). La variabilité annuelle du climat avait une incidence sur la température et le débit des cours d'eau et elle influençait plusieurs caractéristiques biotiques, en particulier la croissance des jeunes de l'année chez l'ombre arctique. Les cours d'eau des Barrenlands ont en commun les caractéristiques fondamentales des cours d'eau de la toundra alaskienne, vu qu'ils sont soumis à des régimes climatiques similaires. Les différences majeures entre les deux régions semblent cependant liées au fait que la décharge des cours d'eau des Barrenlands se fait dans un lac, ce qui pourrait contribuer à une croissance des jeunes de l'année chez l'ombre arctique plus forte que le climat régional ne le laisserait croire

COVID‑19 and SARS‑CoV‑2 host cell entry mediators: Expression profiling of TMRSS4 in health and disease

Copyright: © Katopodis et al. Severe acute respiratory syndrome (SARS) coronavirus‑2 (SARS‑CoV‑2), the causative viral agent for the ongoing COVID‑19 pandemic, enters its host cells primarily via the binding of the SARS‑CoV‑2 spike (S) proteins to the angiotensin‑converting enzyme 2 (ACE2). A number of other cell entry mediators have also been identified, including neuropilin‑1 (NRP1) and transmembrane protease serine 2 (TMPRSS2). More recently, it has been demonstrated that transmembrane protease serine 4 (TMPRSS4) along with TMPRSS2 activate the SARS‑CoV‑2 S proteins, and enhance the viral infection of human small intestinal enterocytes. To date, a systematic analysis of TMPRSS4 in health and disease is lacking. In the present study, using in silico tools, the gene expression and genetic alteration of TMPRSS4 were analysed across numerous tumours and compared to controls. The observations were also expanded to the level of the central nervous system (CNS). The findings revealed that TMPRSS4 was overexpressed in 11 types of cancer, including lung adenocarcinoma, lung squamous cell carcinoma, cervical squamous cell carcinoma, thyroid carcinoma, ovarian cancer, cancer of the rectum, pancreatic cancer, colon and stomach adenocarcinoma, uterine carcinosarcoma and uterine corpus endometrial carcinoma, whilst it was significantly downregulated in kidney carcinomas, acute myeloid leukaemia, skin cutaneous melanoma and testicular germ cell tumours. Finally, a high TMPRSS4 expression was documented in the olfactory tubercle, paraolfactory gyrus and frontal operculum, all brain regions which are associated with the sense of smell and taste. Collectively, these data suggest that TMPRSS4 may play a role in COVID‑19 symptomatology as another SARS‑CoV‑2 host cell entry mediator responsible for the tropism of this coronavirus both in the periphery and the CNS

Fusidic acid and clindamycin resistance in community-associated, methicillin-resistant Staphylococcus aureus infections in children of Central Greece

<p>Abstract</p> <p>Introduction</p> <p>In Greece, fusidic acid and clindamycin are commonly used for the empiric therapy of suspected staphylococcal infections.</p> <p>Methods</p> <p>The medical records of children examined at the outpatient clinics or admitted to the pediatric wards of the University General Hospital of Larissa, Central Greece, with community-associated staphylococcal infections from January 2003 to December 2009 were reviewed.</p> <p>Results</p> <p>Of 309 children (0-14 years old), 21 (6.8%) had invasive infections and 288 (93.2%) skin and soft tissue infections (SSTIs). Thirty-five patients were ≤30 days of age. The proportion of staphylococcal infections caused by a community-associated methicillin-resistant <it>Staphylococcus aureus </it>(CA-MRSA) isolate increased from 51.5% (69 of 134) in 2003-2006 to 63.4% (111 of 175) in 2007-2009 (<it>P </it>= 0.037). Among the CA-MRSA isolates, 88.9% were resistant to fusidic acid, 77.6% to tetracycline, and 21.1% to clindamycin. Clindamycin resistance increased from 0% (2003) to 31.2% (2009) among the CA-MRSA isolates (<it>P </it>= 0.011). Over the 7-year period, an increase in multidrug-resistant CA-MRSA isolates was observed (<it>P </it>= 0.004). One hundred and thirty-one (93.6%) of the 140 tested MRSA isolates were Panton-Valentine leukocidin-positive. Multilocus sequence typing of 72 CA-MRSA isolates revealed that they belonged to ST80 (n = 61), ST30 (n = 6), ST377 (n = 3), ST22 (n = 1), and ST152 (n = 1). Resistance to fusidic acid was observed in ST80 (58/61), ST30 (1/6), and ST22 (1/1) isolates.</p> <p>Conclusion</p> <p>In areas with high rate of infections caused by multidrug-resistant CA-MRSA isolates, predominantly belonging to the European ST80 clone, fusidic acid and clindamycin should be used cautiously as empiric therapy in patients with suspected severe staphylococcal infections.</p

Failure to complete adjuvant chemotherapy is associated with adverse survival in stage III colon cancer patients

Two recent North American studies have shown that completion of 5-fluorouracil (5FU)-based adjuvant chemotherapy is a major prognostic factor for the survival of elderly stage III colon cancer patients. The aim of the present study was to confirm this finding in a population-based series from Australia. The study cohort comprised 851 stage III colon cancer patients treated by surgery alone and 461 who initiated the Mayo chemotherapy regime. One-third of patients who initiated chemotherapy failed to complete more than three cycles of treatment. Independent predictors for failure to complete were treatment in district or rural hospitals, low socioeconomic index and treatment by a low-volume surgeon. Patients who failed to complete chemotherapy showed worse cancer-specific survival compared not only to those who completed treatment (HR=2.24; 95% confidence interval (CI) (1.66–3.03), P<0.001) but also to those treated by surgery alone (HR=1.37; 95% CI (1.09–1.72), P=0.008). The current and previous studies demonstrate the importance of completing adjuvant 5-FU-based chemotherapy for colon cancer. Further prospective studies are required to identify better the physiological and socioeconomic factors responsible for failure to complete chemotherapy so that appropriate improvements in health service delivery can be made

Optical terabit transmitter and receiver based on passive polymer and InP technology for high-speed optical connectivity between datacenters

We demonstrate the hybrid integration of a multi-format tunable transmitter and a coherent optical receiver based on optical polymers and InP electronics and photonics for next generation metro and core optical networks. The transmitter comprises an array of two InP Mach-Zehnder modulators (MZMs) with 42 GHz bandwidth and two passive PolyBoards at the back- and front-end of the device. The back-end PolyBoard integrates an InP gain chip, a Bragg grating and a phase section on the polymer substrate capable of 22 nm wavelength tunability inside the C-band and optical waveguides that guide the light to the inputs of the two InP MZMs. The front-end PolyBoard provides the optical waveguides for combing the In-phase and Quadrature-phase modulated signals via an integrated thermo-optic phase shifter for applying the pi/2 phase-shift at the lower arm and a 3-dB optical coupler at the output. Two InP-double heterojunction bipolar transistor (InP-DHBT) 3-bit power digital-to-analog converters (DACs) are hybridly integrated at either side of the MZM array chip in order to drive the IQ transmitter with QPSK, 16-QAM and 64-QAM encoded signals. The coherent receiver is based on the other side on a PolyBoard, which integrates an InP gain chip and a monolithic Bragg grating for the formation of the local oscillator laser, and a monolithic 90° optical hybrid. This PolyBoard is further integrated with a 4-fold InP photodiode array chip with more than 80 GHz bandwidth and two high-speed InP-DHBT transimpedance amplifiers (TIAs) with automatic gain control. The transmitter and the receiver have been experimentally evaluated at 25Gbaud over 100 km for mQAM modulation showing bit-error-rate (BER) performance performance below FEC limit

Description and performance of track and primary-vertex reconstruction with the CMS tracker

A description is provided of the software algorithms developed for the CMS tracker both for reconstructing charged-particle trajectories in proton-proton interactions and for using the resulting tracks to estimate the positions of the LHC luminous region and individual primary-interaction vertices. Despite the very hostile environment at the LHC, the performance obtained with these algorithms is found to be excellent. For tbar t events under typical 2011 pileup conditions, the average track-reconstruction efficiency for promptly-produced charged particles with transverse momenta of pT > 0.9GeV is 94% for pseudorapidities of |η| < 0.9 and 85% for 0.9 < |η| < 2.5. The inefficiency is caused mainly by hadrons that undergo nuclear interactions in the tracker material. For isolated muons, the corresponding efficiencies are essentially 100%. For isolated muons of pT = 100GeV emitted at |η| < 1.4, the resolutions are approximately 2.8% in pT, and respectively, 10μm and 30μm in the transverse and longitudinal impact parameters. The position resolution achieved for reconstructed primary vertices that correspond to interesting pp collisions is 10–12μm in each of the three spatial dimensions. The tracking and vertexing software is fast and flexible, and easily adaptable to other functions, such as fast tracking for the trigger, or dedicated tracking for electrons that takes into account bremsstrahlung

Alignment of the CMS tracker with LHC and cosmic ray data

© CERN 2014 for the benefit of the CMS collaboration, published under the terms of the Creative Commons Attribution 3.0 License by IOP Publishing Ltd and Sissa Medialab srl. Any further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation and DOI.The central component of the CMS detector is the largest silicon tracker ever built. The precise alignment of this complex device is a formidable challenge, and only achievable with a significant extension of the technologies routinely used for tracking detectors in the past. This article describes the full-scale alignment procedure as it is used during LHC operations. Among the specific features of the method are the simultaneous determination of up to 200 000 alignment parameters with tracks, the measurement of individual sensor curvature parameters, the control of systematic misalignment effects, and the implementation of the whole procedure in a multi-processor environment for high execution speed. Overall, the achieved statistical accuracy on the module alignment is found to be significantly better than 10μm