Indoor Cats, Scratching, and the Debate over Declawing: When Normal Pet Behavior Becomes a Problem

When pet animals share our living spaces, their needs and natural behaviors sometimes are at odds with the varying standards for household appearance, sanitation, and polite social life that Americans have established over time. How pet owners have resolved these issues provides insight into their changing ideas about the role of animals in their households and suggests how much, or how little, people may actually know about the biological behaviors and psychological needs of the creatures they care for. This essay examines one particular issue associated with the problem of sharing spaces: declawing pet cats as a common solution to avoid destructive scratching. This is a volatile issue and has generated much emotional debate. It pits loving cat owners who see such surgery as an act that breaches the trust of responsible pet care for their feline companions against loving cat owners who see the surgery as an act that strengthens their bond with their feline companions. It divides those in the animal welfare and veterinary community as well, where many opinions are believed to be the right opinion. The authors wish to stress that they enjoy the companionship of pet animals in their homes; pointing out the complexities and contradictions in living with pet cats is intended to acknowledge the historical, socially constructed, and changeable character of pet keeping and to encourage people involved in companion animal welfare work to consider why some practices can be promoted or simply tolerated, while others are problematic

Indoor Cats, Scratching, and the Debate over Declawing: When Normal Pet Behavior Becomes a Problem

When pet animals share our living spaces, their needs and natural behaviors sometimes are at odds with the varying standards for household appearance, sanitation, and polite social life that Americans have established over time. How pet owners have resolved these issues provides insight into their changing ideas about the role of animals in their households and suggests how much, or how little, people may actually know about the biological behaviors and psychological needs of the creatures they care for. This essay examines one particular issue associated with the problem of sharing spaces: declawing pet cats as a common solution to avoid destructive scratching. This is a volatile issue and has generated much emotional debate. It pits loving cat owners who see such surgery as an act that breaches the trust of responsible pet care for their feline companions against loving cat owners who see the surgery as an act that strengthens their bond with their feline companions. It divides those in the animal welfare and veterinary community as well, where many opinions are believed to be the right opinion. The authors wish to stress that they enjoy the companionship of pet animals in their homes; pointing out the complexities and contradictions in living with pet cats is intended to acknowledge the historical, socially constructed, and changeable character of pet keeping and to encourage people involved in companion animal welfare work to consider why some practices can be promoted or simply tolerated, while others are problematic

A proposed metric for assessing the measurement quality of individual microarrays

BACKGROUND: High-density microarray technology is increasingly applied to study gene expression levels on a large scale. Microarray experiments rely on several critical steps that may introduce error and uncertainty in analyses. These steps include mRNA sample extraction, amplification and labeling, hybridization, and scanning. In some cases this may be manifested as systematic spatial variation on the surface of microarray in which expression measurements within an individual array may vary as a function of geographic position on the array surface. RESULTS: We hypothesized that an index of the degree of spatiality of gene expression measurements associated with their physical geographic locations on an array could indicate the summary of the physical reliability of the microarray. We introduced a novel way to formulate this index using a statistical analysis tool. Our approach regressed gene expression intensity measurements on a polynomial response surface of the microarray's Cartesian coordinates. We demonstrated this method using a fixed model and presented results from real and simulated datasets. CONCLUSION: We demonstrated the potential of such a quantitative metric for assessing the reliability of individual arrays. Moreover, we showed that this procedure can be incorporated into laboratory practice as a means to set quality control specifications and as a tool to determine whether an array has sufficient quality to be retained in terms of spatial correlation of gene expression measurements

Sources of variation in Affymetrix microarray experiments

BACKGROUND: A typical microarray experiment has many sources of variation which can be attributed to biological and technical causes. Identifying sources of variation and assessing their magnitude, among other factors, are important for optimal experimental design. The objectives of this study were: (1) to estimate relative magnitudes of different sources of variation and (2) to evaluate agreement between biological and technical replicates. RESULTS: We performed a microarray experiment using a total of 24 Affymetrix GeneChip(® )arrays. The study included 4(th )mammary gland samples from eight 21-day-old Sprague Dawley CD female rats exposed to genistein (soy isoflavone). RNA samples from each rat were split to assess variation arising at labeling and hybridization steps. A general linear model was used to estimate variance components. Pearson correlations were computed to evaluate agreement between technical and biological replicates. CONCLUSION: The greatest source of variation was biological variation, followed by residual error, and finally variation due to labeling when *.cel files were processed with dChip and RMA image processing algorithms. When MAS 5.0 or GCRMA-EB were used, the greatest source of variation was residual error, followed by biology and labeling. Correlations between technical replicates were consistently higher than between biological replicates

Project FIT: Rationale, design and baseline characteristics of a school- and community-based intervention to address physical activity and healthy eating among low-income elementary school children

<p>Abstract</p> <p>Background</p> <p>This paper describes Project FIT, a collaboration between the public school system, local health systems, physicians, neighborhood associations, businesses, faith-based leaders, community agencies and university researchers to develop a multi-faceted approach to promote physical activity and healthy eating toward the general goal of preventing and reducing childhood obesity among children in Grand Rapids, MI, USA.</p> <p>Methods/design</p> <p>There are four overall components to Project FIT: school, community, social marketing, and school staff wellness - all that focus on: 1) increasing access to safe and affordable physical activity and nutrition education opportunities in the schools and surrounding neighborhoods; 2) improving the affordability and availability of nutritious food in the neighborhoods surrounding the schools; 3) improving the knowledge, self-efficacy, attitudes and behaviors regarding nutrition and physical activity among school staff, parents and students; 4) impacting the 'culture' of the schools and neighborhoods to incorporate healthful values; and 5) encouraging dialogue among all community partners to leverage existing programs and introduce new ones.</p> <p>Discussion</p> <p>At baseline, there was generally low physical activity (70% do not meet recommendation of 60 minutes per day), excessive screen time (75% do not meet recommendation of < 2 hours per day), and low intake of vegetables and whole grains and high intake of sugar-sweetened beverages, French fries and chips and desserts as well as a high prevalence of overweight and obesity (48.5% including 6% with severe obesity) among low income, primarily Hispanic and African American 3^rd-5^thgrade children (n = 403).</p> <p>Trial registration</p> <p><b>ClinicalTrials.gov <a href="http://www.clinicaltrials.gov/ct2/show/NCT01385046">NCT01385046</a></b></p

EarthFinder Probe Mission Concept Study: Characterizing nearby stellar exoplanet systems with Earth-mass analogs for future direct imaging

EarthFinder is a NASA Astrophysics Probe mission concept selected for study as input to the 2020 Astrophysics National Academies Decadal Survey. The EarthFinder concept is based on a dramatic shift in our understanding of how PRV measurements should be made. We propose a new paradigm which brings the high precision, high cadence domain of transit photometry as demonstrated by Kepler and TESS to the challenges of PRV measurements at the cm/s level. This new paradigm takes advantage of: 1) broad wavelength coverage from the UV to NIR which is only possible from space to minimize the effects of stellar activity; 2) extremely compact, highly stable, highly efficient spectrometers (R>150,000) which require the diffraction-limited imaging possible only from space over a broad wavelength range; 3) the revolution in laser-based wavelength standards to ensure cm/s precision over many years; 4) a high cadence observing program which minimizes sampling-induced period aliases; 5) exploiting the absolute flux stability from space for continuum normalization for unprecedented line-by-line analysis not possible from the ground; and 6) focusing on the bright stars which will be the targets of future imaging missions so that EarthFinder can use a ~1.5 m telescope.Comment: NASA Probe Mission concept white paper for 2020 Astrophysics National Academies Decadal Surve

EarthFinder Probe Mission Concept Study: Characterizing nearby stellar exoplanet systems with Earth-mass analogs for future direct imaging

EarthFinder is a NASA Astrophysics Probe mission concept selected for study as input to the 2020 Astrophysics National Academies Decadal Survey. The EarthFinder concept is based on a dramatic shift in our understanding of how PRV measurements should be made. We propose a new paradigm which brings the high precision, high cadence domain of transit photometry as demonstrated by Kepler and TESS to the challenges of PRV measurements at the cm/s level. This new paradigm takes advantage of: 1) broad wavelength coverage from the UV to NIR which is only possible from space to minimize the effects of stellar activity; 2) extremely compact, highly stable, highly efficient spectrometers (R>150,000) which require the diffraction-limited imaging possible only from space over a broad wavelength range; 3) the revolution in laser-based wavelength standards to ensure cm/s precision over many years; 4) a high cadence observing program which minimizes sampling-induced period aliases; 5) exploiting the absolute flux stability from space for continuum normalization for unprecedented line-by-line analysis not possible from the ground; and 6) focusing on the bright stars which will be the targets of future imaging missions so that EarthFinder can use a ~1.5 m telescope

The Seventeenth Data Release of the Sloan Digital Sky Surveys: Complete Release of MaNGA, MaStar and APOGEE-2 Data

This paper documents the seventeenth data release (DR17) from the Sloan Digital Sky Surveys; the fifth and final release from the fourth phase (SDSS-IV). DR17 contains the complete release of the Mapping Nearby Galaxies at Apache Point Observatory (MaNGA) survey, which reached its goal of surveying over 10,000 nearby galaxies. The complete release of the MaNGA Stellar Library (MaStar) accompanies this data, providing observations of almost 30,000 stars through the MaNGA instrument during bright time. DR17 also contains the complete release of the Apache Point Observatory Galactic Evolution Experiment 2 (APOGEE-2) survey which publicly releases infra-red spectra of over 650,000 stars. The main sample from the Extended Baryon Oscillation Spectroscopic Survey (eBOSS), as well as the sub-survey Time Domain Spectroscopic Survey (TDSS) data were fully released in DR16. New single-fiber optical spectroscopy released in DR17 is from the SPectroscipic IDentification of ERosita Survey (SPIDERS) sub-survey and the eBOSS-RM program. Along with the primary data sets, DR17 includes 25 new or updated Value Added Catalogs (VACs). This paper concludes the release of SDSS-IV survey data. SDSS continues into its fifth phase with observations already underway for the Milky Way Mapper (MWM), Local Volume Mapper (LVM) and Black Hole Mapper (BHM) surveys