9 research outputs found
Porphyrin–Coumarin Dyads: Investigation of Photophysical Properties and DNA Interactions
Two
new nonconjugated porphyrin–coumarin dyads with different orientations
with respect to donor–acceptor entities and their zinc complexes
were synthesized. Single-crystal structures of the free-base porphyrin–coumarin
dyads were successfully resolved. The absorption spectra of the dyads
were linear combinations of the spectra of their corresponding monomers,
indicating a negligible electronic communication between the coumarin
and porphyrin moieties. However, the fluorescence emission of the
coumarin entity in all of the dyads was quenched significantly compared
to that of pristine coumarin, and this effect was attributed to intramolecular
energy transfer from the coumarin to the porphyrin. The energy transfer
kinetics from the coumarin to the porphyrin was shown to be fast (<i>k</i><sub>Förster</sub> = 1.13 × 10<sup>13</sup> s<sup>–1</sup> for the ortho-isomer and 5.13 × 10<sup>11</sup> s<sup>–1</sup> for the para-isomer in DMF) and efficient
(transfer efficiency ca. 96–97%). Transient absorption studies
showed that the excited state decay process (S<sub>2</sub> →
S<sub>1</sub>*, S<sub>1</sub>* → S<sub>1</sub>, S<sub>1</sub> → S<sub>0</sub>, and S<sub>1</sub> → T<sub>1</sub>) of the para-isomer was faster than that of the ortho-isomer in
DMF. All of the synthesized dyads were tested for their interactions
with ct-DNA and photocleavage activity toward PBR322-DNA. The results
revealed that all of the dyads interacted with ct-DNA via only an
external groove-binding mode; the binding constants were calculated
to be 3.24 × 10<sup>5</sup> (<b>3a</b>), 3.05 × 10<sup>5</sup> (<b>3b</b>), 3.04 × 10<sup>5</sup> (<b>4a</b>), and 4.88 × 10<sup>5</sup> (<b>4b</b>), and the photocleavage
activity was in the order <b>4b</b> < <b>3b</b> < <b>4a</b> < <b>3a</b>. Furthermore, only the zinc complexes
of the porphyrin–coumarin dyads could be absorbed by tumor
cells (A549). These complexes were mainly localized in the cytoplasm,
exhibited red fluorescence, and showed low cytotoxicity toward all
of the tumor cell lines tested. The results showed that these zinc
complexes of the porphyrin–coumarin dyads have potential applications
in fluorescence imaging