4 research outputs found
Population genetic data for 17 Y STR markers from Benghazi (East Libya)
The seventeen Y-STR loci included in the AmpF‘STR1 YfilerTM PCR Amplification kit (DYS19, DYS389I,DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS438, DYS439, DYS437, DYS448, DYS458,DYS456, DYS635, and Y-GATA-H4) were used to type a sample population of 238 males from eastern Libya (Benghazi region). Of 238 observed haplotypes, 214 were unique (90%) and 24 (10%) were found more than once. The 17 loci gave a discriminating power of 0.999. DYS458 showed the highest diversity as a single-locus marker (0.73). Allelic frequencies and gene diversities for each Y-STR locus were determined. The high haplotype diversity and discrimination capacity (0.996) demonstrate the utility of
these loci for human identification in forensic applications. Comparative analysis with Y-STR datasets of
relevant populations and submission of the haplotypes to the Y-STR Haplotype Reference Database (YHRD) was undertaken
Toward Male Individualization with Rapidly Mutating Y-Chromosomal Short Tandem Repeats
Peer reviewe
Toward Male Individualization with Rapidly Mutating Y-Chromosomal Short Tandem Repeats
Relevant for various areas of human genetics, Y-chromosomal short tandem repeats (Y-STRs) are commonly used for testing close paternal relationships among individuals and populations, and for male lineage identification. However, even the widely used 17-loci Yfiler set cannot resolve individuals and populations completely. Here, 52 centers generated quality-controlled data of 13 rapidly mutating (RM) Y-STRs in 14,644 related and unrelated males from 111 worldwide populations. Strikingly, >99% of the 12,272 unrelated males were completely individualized. Haplotype diversity was extremely high (global: 0.9999985, regional: 0.99836-0.9999988). Haplotype sharing between populations was almost absent except for six (0.05%) of the 12,156 haplotypes. Haplotype sharing within populations was generally rare (0.8% nonunique haplotypes), significantly lower in urban (0.9%) than rural (2.1%) and highest in endogamous groups (14.3%). Analysi
Toward Male Individualization with Rapidly Mutating Y-Chromosomal Short Tandem Repeats
Relevant for various areas of human genetics,
Y-chromosomal short tandem repeats (Y-STRs) are com-
monly used for testing close paternal relationships among
individuals and populations, and for male lineage iden-
tification. However, even the widely used 17-loci Yfiler
set cannot resolve individuals and populations completely.
Here, 52 centers generated quality-controlled data of 13
rapidly mutating (RM) Y-STRs in 14,644 related and
unrelated males from 111 worldwide populations. Strik-
ingly, >99% of the 12,272 unrelated males were com-
pletely individualized. Haplotype diversity was extremely
high (global: 0.9999985, regional: 0.99836\u20130.9999988).
Haplotype sharing between populations was almost ab-
sent except for six (0.05%) of the 12,156 haplotypes.
Haplotype sharing within populations was generally rare
(0.8% nonunique haplotypes), significantly lower in ur-
ban (0.9%) than rural (2.1%) and highest in endogamous
groups (14.3%). Analysis of molecular variance revealed
99.98% of variation within populations, 0.018% among
populations within groups, and 0.002% among groups. Of
the 2,372 newly and 156 previously typed male relative
pairs,29% were differentiated including 27% of the 2,378
father\u2013son pairs. Relative to Yfiler, haplotype diversity
was increased in 86% of the populations tested and over-
all male relative differentiation was raised by 23.5%. Our
study demonstrates the value of RM Y-STRs in identifying
and separating unrelated and related males and provides a
reference database