412 research outputs found
A rapid method for isolation of total nucleic acids from Aspergillus nidulans
With the development of molecular biology techniques and their application to the analysis of cellular events, the isolation of total nucleic acids in Aspergillus nidulans for Southern and Northern hybridization has become routine
Lethality or improved growth as a result of interaction in DNA repair double mutants of Aspergillus
Genetic analysis recently identified nine new DNA repair genes of Aspergillus nidulans, musK - musS, which map on several different chromosomes (Käfer 1994, preceding article in this Newsletter). Such mus mutants are sensitive to certain chemical mutagens, but not sensitive or only slightly sensitive to UV and gamma-radiation. To identify epistatic interactions with members of the 4 Uvs groups, double mutants strains were isolated (Käfer and Chae 1994 Curr. Genet. 25:223-232). However, some mus;uvs double mutants could not be analyzed because they grew too poorly or were lethal (showing synthetic enhancement in gene interaction ; Guarente 1993 Trends Genet. 9:362-366). The opposite effect was also found; namely interaction which led to improved recovery and growth (or rescue ) as documented here
Pre-rehabilitation sense of coherence as a predictor of symptom change after rehabilitation
Sense of coherence (SOC) constitutes the key component of salutogenesis theory. It reflects individuals' confidence that their environment is comprehensible and manageable and that their lives are meaningful. Research demonstrates a strong cross-sectional relationship between SOC and mental health. However, little is known about SOC's temporal stability and its potential to predict changes in psychopathological symptom severity in different settings. The goal of the current study was to address this gap. The sample of the two-wave longitudinal study consists of 294 patients receiving inpatient psychotherapeutic (and psychopharmacological) treatment for various psychological disorders at a German psychosomatic rehabilitation clinic. SOC (Antonovsky, Social Science & Medicine, 1993, 36, 725-733) and outcome measures (i.e., general mental health problems, depression and anxiety symptoms) were assessed within two days of arrival and at the end of rehabilitation (week 5/6). SOC was significantly enhanced after treatment whereas psychopathological symptoms were significantly reduced. Regression analyses revealed that pre-treatment SOC was a significant negative predictor of post-treatment symptom severity for all outcome measures even after controlling for pre-treatment symptoms. The current findings provide first evidence that SOC is a significant unique predictor of symptom change. Future studies need to further investigate longitudinal associations between SOC and mental health outcomes in different settings
Influence of steps on the tilting and adsorption dynamics of ordered Pn films on vicinal Ag(111) surfaces
Here we present a structural study of pentacene (Pn) thin films on vicinal
Ag(111) surfaces by He atom diffraction measurements and density functional
theory (DFT) calculations supplemented with van der Waals (vdW) interactions.
Our He atom diffraction results suggest initial adsorption at the step edges
evidenced by initial slow specular reflection intensity decay rate as a
function of Pn deposition time. In parallel with the experimental findings, our
DFT+vdW calculations predict the step edges as the most stable adsorption site
on the surface. An isolated molecule adsorbs as tilted on the step edge with a
binding energy of 1.4 eV. In addition, a complete monolayer (ML) with
pentacenes flat on the terraces and tilted only at the step edges is found to
be more stable than one with all lying flat or tilted molecules, which in turn
influences multilayers. Hence our results suggest that step edges can trap Pn
molecules and act as nucleation sites for the growth of ordered thin films with
a crystal structure similar to that of bulk Pn.Comment: 4 pages, 4 figures, 1 tabl
Chronic y-secretase inhibition reduces amyloid plaque-associated instability of pre- and postsynaptic structures
The loss of synapses is a strong histological correlate of the cognitive decline in Alzheimer’s disease (AD). Amyloid bpeptide (Ab), a cleavage product of the amyloid precursor protein (APP), exerts detrimental effects on synapses, a process thought to be causally related to the cognitive deficits in AD. Here, we used in vivo two-photon microscopy to characterize the dynamics of axonal boutons and dendritic spines in APP/Presenilin 1 (APPswe/PS1L166P)–green fluorescent protein (GFP) transgenic mice. Time-lapse imaging over 4 weeks revealed a pronounced, concerted instability of pre- and postsynaptic structures within the vicinity of amyloid plaques. Treatment with a novel sulfonamide-type g-secretase inhibitor (GSI) attenuated the formation and growth of new plaques and, most importantly, led to a normalization of the enhanced dynamics of synaptic structures close to plaques. GSI treatment did neither affect spines and boutons distant from plaques in amyloid precursor protein/presenilin 1-GFP (APPPS1-GFP) nor those in GFP-control mice, suggesting no obvious neuropathological side effects of the drug
Mechanical Stress Inference for Two Dimensional Cell Arrays
Many morphogenetic processes involve mechanical rearrangement of epithelial
tissues that is driven by precisely regulated cytoskeletal forces and cell
adhesion. The mechanical state of the cell and intercellular adhesion are not
only the targets of regulation, but are themselves likely signals that
coordinate developmental process. Yet, because it is difficult to directly
measure mechanical stress {\it in vivo} on sub-cellular scale, little is
understood about the role of mechanics of development. Here we present an
alternative approach which takes advantage of the recent progress in live
imaging of morphogenetic processes and uses computational analysis of high
resolution images of epithelial tissues to infer relative magnitude of forces
acting within and between cells. We model intracellular stress in terms of bulk
pressure and interfacial tension, allowing these parameters to vary from cell
to cell and from interface to interface. Assuming that epithelial cell layers
are close to mechanical equilibrium, we use the observed geometry of the two
dimensional cell array to infer interfacial tensions and intracellular
pressures. Here we present the mathematical formulation of the proposed
Mechanical Inverse method and apply it to the analysis of epithelial cell
layers observed at the onset of ventral furrow formation in the {\it
Drosophila} embryo and in the process of hair-cell determination in the avian
cochlea. The analysis reveals mechanical anisotropy in the former process and
mechanical heterogeneity, correlated with cell differentiation, in the latter
process. The method opens a way for quantitative and detailed experimental
tests of models of cell and tissue mechanics
Monitoring of the operating parameters of the KATRIN Windowless Gaseous Tritium Source
The Karlsruhe Tritium Neutrino (KATRIN) experiment will measure the absolute
mass scale of neutrinos with a sensitivity of \m_{\nu} = 200 meV/c by
high-precision spectroscopy close to the tritium beta-decay endpoint at 18.6
keV. Its Windowless Gaseous Tritium Source (WGTS) is a beta-decay source of
high intensity (/s) and stability, where high-purity molecular tritium
at 30 K is circulated in a closed loop with a yearly throughput of 10 kg. To
limit systematic effects the column density of the source has to be stabilised
at the 0.1% level. This requires extensive sensor instrumentation and dedicated
control and monitoring systems for parameters such as the beam tube
temperature, injection pressure, gas composition and others. Here we give an
overview of these systems including a dedicated Laser-Raman system as well as
several beta-decay activity monitors. We also report on results of the WGTS
demonstrator and other large-scale test experiments giving proof-of-principle
that all parameters relevant to the systematics can be controlled and monitored
on the 0.1% level or better. As a result of these works, the WGTS systematics
can be controlled within stringent margins, enabling the KATRIN experiment to
explore the neutrino mass scale with the design sensitivity.Comment: 32 pages, 13 figures. modification to title, typos correcte
Measurement of Semileptonic Branching Fractions of B Mesons to Narrow D** States
Using the data accumulated in 2002-2004 with the DO detector in
proton-antiproton collisions at the Fermilab Tevatron collider with
centre-of-mass energy 1.96 TeV, the branching fractions of the decays B ->
\bar{D}_1^0(2420) \mu^+ \nu_\mu X and B -> \bar{D}_2^{*0}(2460) \mu^+ \nu_\mu X
and their ratio have been measured: BR(\bar{b}->B) \cdot BR(B-> \bar{D}_1^0
\mu^+ \nu_\mu X) \cdot BR(\bar{D}_1^0 -> D*- pi+) =
(0.087+-0.007(stat)+-0.014(syst))%; BR(\bar{b}->B)\cdot BR(B->D_2^{*0} \mu^+
\nu_\mu X) \cdot BR(\bar{D}_2^{*0} -> D*- \pi^+) =
(0.035+-0.007(stat)+-0.008(syst))%; and (BR(B -> \bar{D}_2^{*0} \mu^+ \nu_\mu
X)BR(D2*0->D*- pi+)) / (BR(B -> \bar{D}_1^{0} \mu^+ \nu_\mu X)\cdot
BR(\bar{D}_1^{0}->D*- \pi^+)) = 0.39+-0.09(stat)+-0.12(syst), where the charge
conjugated states are always implied.Comment: submitted to Phys. Rev. Let
Search for Large Extra Spatial Dimensions in Dimuon Production with the D0 Detector
We present the results of a search for the effects of large extra spatial
dimensions in collisions at 1.96 TeV in events
containing a pair of energetic muons. The data correspond to 246 \ipb of
integrated luminosity collected by the \D0 experiment at the Fermilab Tevatron
Collider. Good agreement with the expected background was found, yielding no
evidence for large extra dimensions. We set 95% C.L. lower limits on the
fundamental Planck scale between 0.85 TeV and 1.27 TeV within several
formalisms. These are the most stringent limits achieved in the dimuon channel
to date.Comment: 8 pages, 3 figures, 1 table. Published in Phys. Rev. Lett. Minor
changes in v2 to match the published versio
Measurement of the Collins and Sivers asymmetries on transversely polarised protons
The Collins and Sivers asymmetries for charged hadrons produced in deeply
inelastic scattering on transversely polarised protons have been extracted from
the data collected in 2007 with the CERN SPS muon beam tuned at 160 GeV/c. At
large values of the Bjorken x variable non-zero Collins asymmetries are
observed both for positive and negative hadrons while the Sivers asymmetry for
positive hadrons is slightly positive over almost all the measured x range.
These results nicely support the present theoretical interpretation of these
asymmetries, in terms of leading-twist quark distribution and fragmentation
functions.Comment: 9 Pages, 5 figure
- …