Population ecology and lifetime reproductive success of dippers Cinclus cinclus

Acidified catchments are known to hold significantly reduced dipper Cinclus cinclus populations throughout the year relative to circum-neutral rivers, although the processes leading to these declines remain unclear. This study considered the population ecology of dippers within the circumneutral River Devon catchment, Central Scotland, and focused primarily on determining the factors influencing survival, breeding probabilities and reproductive success. It aimed to examine the role of spatial variation in 'habitat quality' on the population (and meta-population) dynamics of dippers, based on measures of seasonal and lifetime reproductive success, and the balance between survival and reproduction; in particular, to assess if the reduced reproductive success of dippers on acid rivers is likely to lead to population declines. Within the Devon catchment, approximately 81% of all adults survived from spring (March/April) to autumn (September/October), with 65% of these birds surviving from autumn to the following breeding season. Overall, these estimates predicted annual adult survival rates of c.53%, with no significant differences between years. Population density had no detectable effect on adult mortality rates, although juvenile over-winter survival was significantly lower than the adult rate at between 40 and 58%, and negatively related to the total size of the autumn population. There was no evidence of sex differences in juvenile over-winter survival, or any significant influence of weather or river flows on the rates for adults or juveniles. The local post-fledging survival of females was significantly lower than for males, however, apparently reflecting sex differences in post-natal dispersal. On average, less than 6.5% of all eggs laid, or 10.4-14.5% of male and 6.3-9.2% of female fledglings raised within the Devon catchment survived locally to breeding age. Juvenile, although not adult, recapture rates in spring were significantly lower than for birds known to have bred previously and negatively related to spring river flows. This suggested that with recapture dependent on a breeding attempt that was successful at least until laying, either more first year birds failed during the initial stages of nesting or that full breeding was not achieved at age one. The birds fledging the most young, both within a season and over a lifetime, all bred at 'prime' lowland sites characterised by wide, shallow rivers of intermediate gradient, although with less than 10% of all birds attempting to raise a second brood each year, no significant habitat differences were identified in any component of reproductive output measured until fledging. River width, altitude and gradient were all significantly inter-correlated and related to laying date, however, and post-fledging survival was significantly reduced for late fledged young. On average, lowland birds laid earlier than upland breeders, and were significantly more likely to produce autumn 'recruits' due to the enhanced post-fledging survival prospects of their young. This suggested that broad measures of river structure can provide a biologically appropriate classification of habitat quality. The size of the breeding population of dippers within the Devon catchment appeared to be related to the availability of critical resources, most likely food, roost sites and ultimately breeding territories through density-dependent changes in over-winter mortality and recruitment. The relative importance of resource abundance and recruitment levels in determining autumn population densities on acid streams still remained unclear, although reference to published relationships between acidity and reproductive success suggested that with adult survival at the rate estimated for the Devon catchment, many dipper populations are unlikely to produce sufficient recruits to match all adult losses, and may only persist with continued immigration from more productive (circumneutral) catchments elsewhere

An Asymmetric Eclipse Seen toward the Pre-main-sequence Binary System V928 Tau

K2 observations of the weak-lined T Tauri binary V928 Tau A and B show the detection of a single, asymmetric eclipse, which may be due to a previously unknown substellar companion eclipsing one component of the binary with an orbital period >66 days. Over an interval of about 9 hr, one component of the binary dims by around 60%, returning to its normal brightness about 5 hr later. From modeling of the eclipse shape, we find evidence that the eclipsing companion may be surrounded by a disk or a vast ring system. The modeled disk has a radius of 0.9923 ± 0.0005 R*, with an inclination of 56 78 ± 0 03, a tilt of 41 22 ± 0 05, an impact parameter of −0.2506 ± 0.0002 R*, and an opacity of 1.00. The occulting disk must also move at a transverse velocity of 6.637 ± 0.002 R* day⁻¹, which, depending on whether it orbits V928 Tau A or B, corresponds to approximately 73.53 or 69.26 km s⁻¹. A search in ground-based archival data reveals additional dimming events, some of which suggest periodicity, but no unambiguous period associated with the eclipse observed by K2. We present a new epoch of astrometry that is used to further refine the orbit of the binary, presenting a new lower bound of 67 yr, and constraints on the possible orbital periods of the eclipsing companion. The binary is also separated by 18'' (~2250 au) from the lower-mass CFHT-BD-Tau 7, which is likely associated with V928 Tau A and B. We also present new high-dispersion optical spectroscopy that we use to characterize the unresolved stellar binary

Interaction effects on common measures of sensitivity:Choice of measure, type I error, and power

Here we use simulation to assess previously unaddressed problems in the assessment of statistical interactions in detection and recognition tasks. The proportion of hits and false-alarms made by an observer on such tasks is affected by both their sensitivity and bias, and numerous measures have been developed to separate out these two factors. Each of these measures makes different assumptions regarding the underlying process and different predictions as to how false-alarm and hit rates should covary. Previous simulations have shown that choice of an inappropriate measure can lead to inflated type I error rates, or reduced power, for main effects, provided there are differences in response bias between the conditions being compared. Interaction effects pose a particular problem in this context. We show that spurious interaction effects in analysis of variance can be produced, or true interactions missed, even in the absence of variation in bias. Additional simulations show that variation in bias complicates patterns of type I error and power further. This under-appreciated fact has the potential to greatly distort the assessment of interactions in detection and recognition experiments. We discuss steps researchers can take to mitigate their chances of making an error

Genetic Identification of a Network of Factors that Functionally Interact with the Nucleosome Remodeling ATPase ISWI

Nucleosome remodeling and covalent modifications of histones play fundamental roles in chromatin structure and function. However, much remains to be learned about how the action of ATP-dependent chromatin remodeling factors and histone-modifying enzymes is coordinated to modulate chromatin organization and transcription. The evolutionarily conserved ATP-dependent chromatin-remodeling factor ISWI plays essential roles in chromosome organization, DNA replication, and transcription regulation. To gain insight into regulation and mechanism of action of ISWI, we conducted an unbiased genetic screen to identify factors with which it interacts in vivo. We found that ISWI interacts with a network of factors that escaped detection in previous biochemical analyses, including the Sin3A gene. The Sin3A protein and the histone deacetylase Rpd3 are part of a conserved histone deacetylase complex involved in transcriptional repression. ISWI and the Sin3A/Rpd3 complex co-localize at specific chromosome domains. Loss of ISWI activity causes a reduction in the binding of the Sin3A/Rpd3 complex to chromatin. Biochemical analysis showed that the ISWI physically interacts with the histone deacetylase activity of the Sin3A/Rpd3 complex. Consistent with these findings, the acetylation of histone H4 is altered when ISWI activity is perturbed in vivo. These findings suggest that ISWI associates with the Sin3A/Rpd3 complex to support its function in vivo

Variation in the Glucose Transporter gene <i>SLC2A2 </i>is associated with glycaemic response to metformin

Metformin is the first-line antidiabetic drug with over 100 million users worldwide, yet its mechanism of action remains unclear1. Here the Metformin Genetics (MetGen) Consortium reports a three-stage genome-wide association study (GWAS), consisting of 13,123 participants of different ancestries. The C allele of rs8192675 in the intron of SLC2A2, which encodes the facilitated glucose transporter GLUT2, was associated with a 0.17% (P = 6.6 × 10−14) greater metformin-induced reduction in hemoglobin A1c (HbA1c) in 10,577 participants of European ancestry. rs8192675 was the top cis expression quantitative trait locus (cis-eQTL) for SLC2A2 in 1,226 human liver samples, suggesting a key role for hepatic GLUT2 in regulation of metformin action. Among obese individuals, C-allele homozygotes at rs8192675 had a 0.33% (3.6 mmol/mol) greater absolute HbA1c reduction than T-allele homozygotes. This was about half the effect seen with the addition of a DPP-4 inhibitor, and equated to a dose difference of 550 mg of metformin, suggesting rs8192675 as a potential biomarker for stratified medicine

The First Post-Kepler Brightness Dips of KIC 8462852

We present a photometric detection of the first brightness dips of the unique variable star KIC 8462852 since the end of the Kepler space mission in 2013 May. Our regular photometric surveillance started in October 2015, and a sequence of dipping began in 2017 May continuing on through the end of 2017, when the star was no longer visible from Earth. We distinguish four main 1-2.5% dips, named "Elsie," "Celeste," "Skara Brae," and "Angkor", which persist on timescales from several days to weeks. Our main results so far are: (i) there are no apparent changes of the stellar spectrum or polarization during the dips; (ii) the multiband photometry of the dips shows differential reddening favoring non-grey extinction. Therefore, our data are inconsistent with dip models that invoke optically thick material, but rather they are in-line with predictions for an occulter consisting primarily of ordinary dust, where much of the material must be optically thin with a size scale <<1um, and may also be consistent with models invoking variations intrinsic to the stellar photosphere. Notably, our data do not place constraints on the color of the longer-term "secular" dimming, which may be caused by independent processes, or probe different regimes of a single process

Species-specific regulation of angiogenesis by glucocorticoids reveals contrasting effects on inflammatory and angiogenic pathways

<div><p>Glucocorticoids are potent inhibitors of angiogenesis in the rodent <i>in vivo</i> and <i>in vitro</i> but the mechanism by which this occurs has not been determined. Administration of glucocorticoids is used to treat a number of conditions in horses but the angiogenic response of equine vessels to glucocorticoids and, therefore, the potential role of glucocorticoids in pathogenesis and treatment of equine disease, is unknown. This study addressed the hypothesis that glucocorticoids would be angiostatic both in equine and murine blood vessels.The mouse aortic ring model of angiogenesis was adapted to assess the effects of cortisol in equine vessels. Vessel rings were cultured under basal conditions or exposed to: foetal bovine serum (FBS; 3%); cortisol (600 nM), cortisol (600nM) plus FBS (3%), cortisol (600nM) plus either the glucocorticoid receptor antagonist RU486 or the mineralocorticoid receptor antagonist spironolactone. In murine aortae cortisol inhibited and FBS stimulated new vessel growth. In contrast, in equine blood vessels FBS alone had no effect but cortisol alone, or in combination with FBS, dramatically increased new vessel growth compared with controls. This effect was blocked by glucocorticoid receptor antagonism but not by mineralocorticoid antagonism. The transcriptomes of murine and equine angiogenesis demonstrated cortisol-induced down-regulation of inflammatory pathways in both species but up-regulation of pro-angiogenic pathways selectively in the horse. Genes up-regulated in the horse and down-regulated in mice were associated with the extracellular matrix. These data call into question our understanding of glucocorticoids as angiostatic in every species and may be of clinical relevance in the horse.</p></div