Protective Efficacy against Respiratory Syncytial Virus following Murine Neonatal Immunization with BBG2Na Vaccine: Influence of Adjuvants and Maternal Antibodies

Alum-adsorbed BBG2Na, a recombinant vaccine derived in part from the respiratory syncytial virus (RSV) subgroup A G protein, induced moderate antibody titers after 1 immunization in 1-week-old mice but conferred complete lung protection upon RSV challenge. The anti-BBG2Na IgG1-IgG2a neonatal isotype profile was suggestive of dominant Th2 responses compared with those in adults. Formulation of BBG2Na with a Th1-driving adjuvant efficiently shifted neonatal responses toward a more balanced and adultlike IgG1-IgG2a profile without compromising its protective efficacy. BBG2Na-induced protective immunity was maintained even after early life immunization in the presence of high titers of maternal antibodies. Under these conditions, the protective efficacy (86%-100%) reflected the high capacity of the nonglycosylated G2Na immunogen to escape inhibition by RSV-A—induced maternal antibodies. Thus, immunization with BBG2Na protected against viral challenge despite neonatal immunologic immaturity and the presence of maternal antibodies, two major obstacles to neonatal RSV vaccine developmen

The Respiratory Syncytial Virus G Protein Conserved Domain Induces a Persistent and Protective Antibody Response in Rodents

Respiratory syncytial virus (RSV) is an important cause of severe upper and lower respiratory disease in infants and in the elderly. There are 2 main RSV subtypes A and B. A recombinant vaccine was designed based on the central domain of the RSV-A attachment G protein which we had previously named G2Na (aa130–230). Here we evaluated immunogenicity, persistence of antibody (Ab) response and protective efficacy induced in rodents by: (i) G2Na fused to DT (Diphtheria toxin) fragments in cotton rats. DT fusion did not potentiate neutralizing Ab responses against RSV-A or cross-reactivity to RSV-B. (ii) G2Nb (aa130–230 of the RSV-B G protein) either fused to, or admixed with G2Na. G2Nb did not induce RSV-B-reactive Ab responses. (iii) G2Na at low doses. Two injections of 3 µg G2Na in Alum were sufficient to induce protective immune responses in mouse lungs, preventing RSV-A and greatly reducing RSV-B infections. In cotton rats, G2Na-induced RSV-reactive Ab and protective immunity against RSV-A challenge that persisted for at least 24 weeks. (iv) injecting RSV primed mice with a single dose of G2Na/Alum or G2Na/PLGA [poly(D,L-lactide-co-glycolide]. Despite the presence of pre-existing RSV-specific Abs, these formulations effectively boosted anti-RSV Ab titres and increased Ab titres persisted for at least 21 weeks. Affinity maturation of these Abs increased from day 28 to day 148. These data indicate that G2Na has potential as a component of an RSV vaccine formulation

The Grizzly, March 20, 1987

Pledging Population Plummets • Resident Fulbright Scholar to Lecture on Latin America • Board Makes Promotions • Letter: Help is on the Way Writes Jamison • Upcoming Meistersinger and Chamber Singer Concert • Our Town Student Actor Profiles • Senior Symp. Surges Ahead • Women\u27s Lax Set to Defend Title on Wednesday • Bear Baseball Rides 5-1 Florida Trip into Collegeville • Track Opens • Young Softball Team Begins New Season •Swimmin\u27 Women Medal Winners • Infant Women\u27s Running Program Soars Over the MAC • Former Olympic Player to Fill Soccer Assistant Slot •Sieracki Steps Down after Seven Years • Deep Purple Releases Blue Light LPhttps://digitalcommons.ursinus.edu/grizzlynews/1185/thumbnail.jp

The Grizzly, May 1, 1987

Will Ursinus Make the Grade? • Commuters Recognized • Student Apathy a Farce • Beatles Now Available on Disc • Dougy is King to Sig Rho • Notes: Summer Tennis Strategies Offered; Continuing Ed. Becomes Assertive; Physical Education Day: May 4th; Band and Jazz Ensemble to Perform • Lacrosse Shoots for 4th Title; Bingaman Breaks Another Record • MAC\u27s Return to Ursinus; Bears Look to Usurp Crown • Golf Ties Record at 15-1: Klee, Ignatowicz Lead Bears to MAC Fifth • Trout Tourney Results • The Men Looking For Glory • Baseball Season Ends on Down Note • Kulp Driven to Excellence • Softball Denied Playoffs • Women Netters .500 • Dolman Leaves with Warm Feelings • Renovations at Myrin • Zucker Retires to Further Musical Interests • Multi-talented Symons Ends Forty Year Career at Ursinus • Page Closes the Book at Ursinushttps://digitalcommons.ursinus.edu/grizzlynews/1189/thumbnail.jp

Management for sustainable cephalopod fisheries in Europe: review and recommendations

Cephalopod International Advisory Council Conference, Cephalopods in the Anthropocene: Multiple Challenges in a Changing Ocean, April 2-8, 2022, Sesimbra, PortugalAlthough cephalopod fisheries are of world-wide importance, in Europe catching cephalopods is managed only in small-scale fisheries, at national level, and few stocks are formally assessed. Because cephalopod are not quota species under the EU’s Common Fisheries Policy, there is currently no requirement for assessment or management at European level. Given increasing interest in targeting cephalopods in Europe, there is a risk that they will be fished unsustainably. Although there have been recent review papers on progress in stock assessment and fishery forecasting for commercially fished cephalopods there has been no recent review of cephalopod fishery management. We aim to fill this gap, with a particular focus on European cephalopod fisheries.We review potential barriers to sustainable fishing and reasons why management of cephalopod fisheries differs from that for finfish fisheries, e.g. due to the high inherent volatility and the possibly cyclic nature of year-to-year variation in cephalopod abundance, reflecting their short lifespan, rapid growth and high sensitivity to environmental conditions. We review fishery management approaches in important cephalopod fisheries worldwide (e.g. in the USA, Japan, Falklands, South Africa, Australia and Russia) and current management of small-scale cephalopod fisheries in Europe. We identify knowledge gaps and limitations to current monitoring programmes and stock assessments and discuss the options available for cephalopod fishery management in Europe, considering the suitability or otherwise of catch and effort limits, use of closed areas and seasons, restrictions on sizes caught and types of fishing gear, and the ole of market-based sustainability pathwaysN

Novel loci for childhood body mass index and shared heritability with adult cardiometabolic traits

The genetic background of childhood body mass index (BMI), and the extent to which the well-known associations of childhood BMI with adult diseases are explained by shared genetic factors, are largely unknown. We performed a genome-wide association study meta-analysis of BMI in 61,111 children aged between 2 and 10 years. Twenty-five independent loci reached genome-wide significance in the combined discovery and replication analyses. Two of these, located nearNEDD4LandSLC45A3, have not previously been reported in relation to either childhood or adult BMI. Positive genetic correlations of childhood BMI with birth weight and adult BMI, waist-to-hip ratio, diastolic blood pressure and type 2 diabetes were detected (R(g)ranging from 0.11 to 0.76, P-values Author summary Although twin studies have shown that body mass index (BMI) is highly heritable, many common genetic variants involved in the development of BMI have not yet been identified, especially in children. We studied associations of more than 40 million genetic variants with childhood BMI in 61,111 children aged between 2 and 10 years. We identified 25 genetic variants that were associated with childhood BMI. Two of these have not been implicated for BMI previously, located close to the genesNEDD4LandSLC45A3. We also show that the genetic background of childhood BMI overlaps with that of birth weight, adult BMI, waist-to-hip-ratio, diastolic blood pressure, type 2 diabetes, and age at menarche. Our results suggest that the biological processes underlying childhood BMI largely overlap with those underlying adult BMI. However, the overlap is not complete. Additionally, the genetic backgrounds of childhood BMI and other cardio-metabolic phenotypes are overlapping. This may mean that the associations of childhood BMI and later cardio-metabolic outcomes are partially explained by shared genetics, but it could also be explained by the strong association of childhood BMI with adult BMI.Peer reviewe

New loci associated with birth weight identify genetic links between intrauterine growth and adult height and metabolism.

Birth weight within the normal range is associated with a variety of adult-onset diseases, but the mechanisms behind these associations are poorly understood. Previous genome-wide association studies of birth weight identified a variant in the ADCY5 gene associated both with birth weight and type 2 diabetes and a second variant, near CCNL1, with no obvious link to adult traits. In an expanded genome-wide association meta-analysis and follow-up study of birth weight (of up to 69,308 individuals of European descent from 43 studies), we have now extended the number of loci associated at genome-wide significance to 7, accounting for a similar proportion of variance as maternal smoking. Five of the loci are known to be associated with other phenotypes: ADCY5 and CDKAL1 with type 2 diabetes, ADRB1 with adult blood pressure and HMGA2 and LCORL with adult height. Our findings highlight genetic links between fetal growth and postnatal growth and metabolism

Sensory Communication

Contains table of contents for Section 2, an introduction and reports on twelve research projects.National Institutes of Health Grant R01 DC00117National Institutes of Health Grant R01 DC02032National Institutes of Health/National Institute of Deafness and Other Communication Disorders Grant 2 R01 DC00126National Institutes of Health Grant 2 R01 DC00270National Institutes of Health Contract N01 DC-5-2107National Institutes of Health Grant 2 R01 DC00100U.S. Navy - Office of Naval Research Grant N61339-96-K-0002U.S. Navy - Office of Naval Research Grant N61339-96-K-0003U.S. Navy - Office of Naval Research Grant N00014-97-1-0635U.S. Navy - Office of Naval Research Grant N00014-97-1-0655U.S. Navy - Office of Naval Research Subcontract 40167U.S. Navy - Office of Naval Research Grant N00014-96-1-0379U.S. Air Force - Office of Scientific Research Grant F49620-96-1-0202National Institutes of Health Grant RO1 NS33778Massachusetts General Hospital, Center for Innovative Minimally Invasive Therapy Research Fellowship Gran

Sensory Communication

Contains table of contents for Section 2, an introduction and reports on fourteen research projects.National Institutes of Health Grant RO1 DC00117National Institutes of Health Grant RO1 DC02032National Institutes of Health/National Institute on Deafness and Other Communication Disorders Grant R01 DC00126National Institutes of Health Grant R01 DC00270National Institutes of Health Contract N01 DC52107U.S. Navy - Office of Naval Research/Naval Air Warfare Center Contract N61339-95-K-0014U.S. Navy - Office of Naval Research/Naval Air Warfare Center Contract N61339-96-K-0003U.S. Navy - Office of Naval Research Grant N00014-96-1-0379U.S. Air Force - Office of Scientific Research Grant F49620-95-1-0176U.S. Air Force - Office of Scientific Research Grant F49620-96-1-0202U.S. Navy - Office of Naval Research Subcontract 40167U.S. Navy - Office of Naval Research/Naval Air Warfare Center Contract N61339-96-K-0002National Institutes of Health Grant R01-NS33778U.S. Navy - Office of Naval Research Grant N00014-92-J-184