Remarks to the ecology of the boreo-montane polypore Amylocystis lapponica based on data from the Czech Republic and Poland

In the Czech Republic, the rare polypore Amylocystis lapponica continuously occurs in the Boubínský prales virgin forest (southern Bohemia: Šumava Mts.) where it has been documented for more than 60 years. Similarly, in Poland it has been known only from the Puszcza Białowieska virgin forest (northeastern Poland) for more than 50 years. Generally, it is considered a species of boreal coniferous forests of northern Europe (taiga) and montane coniferous forests in Central and Southern Europe. However, the data from the Czech Republic, Slovakia and western Ukraine show that it also grows in mixed montane forests composed mainly of Fagus sylvatica, Picea abies and Abies alba. In Poland, the locality is situated in a lowland forest consisting mostly of Carpinus betulus, Quercus robur and Tilia cordata. In Central Europe, A. lapponica occurs only in virgin forest refuges with the following habitat conditions: vegetation continuity (never cut), natural tree species composition, multi-aged structure, rich presence of dead wood in various stages of decay, relatively large area of the virgin forest surrounded by near-natural forests, stable, cold and humid meso- and microclimate. Consequently, A. lapponica may be considered an indicator of long-term vegetation continuity and stable habitat conditions

Cyphelloporia bialoviesensis (Fungi, Agaricales)—a new genus and species for a giant cyphelloid fungus from Białowieża virgin forest in Poland

Karasiński, Dariusz, Nagy, László G., Szarkándi, János Gergö, Dvořák, Daniel, Kolařík, Miroslav, Holec, Jan (2023): Cyphelloporia bialoviesensis (Fungi, Agaricales)-a new genus and species for a giant cyphelloid fungus from Białowieża virgin forest in Poland. Phytotaxa 589 (2): 119-136, DOI: 10.11646/phytotaxa.589.2.3, URL: http://dx.doi.org/10.11646/phytotaxa.589.2.

Organelle trafficking of chimeric ribozymes and genetic manipulation of mitochondria

With the expansion of the RNA world, antisense strategies have become widespread to manipulate nuclear gene expression but organelle genetic systems have remained aside. The present work opens the field to mitochondria. We demonstrate that customized RNAs expressed from a nuclear transgene and driven by a transfer RNA-like (tRNA-like) moiety are taken up by mitochondria in plant cells. The process appears to follow the natural tRNA import specificity, suggesting that translocation indeed occurs through the regular tRNA uptake pathway. Upon validation of the strategy with a reporter sequence, we developed a chimeric catalytic RNA composed of a specially designed trans-cleaving hammerhead ribozyme and a tRNA mimic. Organelle import of the chimeric ribozyme and specific target cleavage within mitochondria were demonstrated in transgenic tobacco cell cultures and Arabidopsis thaliana plants, providing the first directed knockdown of a mitochondrial RNA in a multicellular eukaryote. Further observations point to mitochondrial messenger RNA control mechanisms related to the plant developmental stage and culture conditions. Transformation of mitochondria is only accessible in yeast and in the unicellular alga Chlamydomonas. Based on the widespread tRNA import pathway, our data thus make a breakthrough for direct investigation and manipulation of mitochondrial genetics

A unified phylogeny-based nomenclature for histone variants

Histone variants are non-allelic protein isoforms that play key roles in diversifying chromatin structure. The known number of such variants has greatly increased in recent years, but the lack of naming conventions for them has led to a variety of naming styles, multiple synonyms and misleading homographs that obscure variant relationships and complicate database searches. We propose here a unified nomenclature for variants of all five classes of histones that uses consistent but flexible naming conventions to produce names that are informative and readily searchable. The nomenclature builds on historical usage and incorporates phylogenetic relationships, which are strong predictors of structure and function. A key feature is the consistent use of punctuation to represent phylogenetic divergence, making explicit the relationships among variant subtypes that have previously been implicit or unclear. We recommend that by default new histone variants be named with organism-specific paralog-number suffixes that lack phylogenetic implication, while letter suffixes be reserved for structurally distinct clades of variants. For clarity and searchability, we encourage the use of descriptors that are separate from the phylogeny-based variant name to indicate developmental and other properties of variants that may be independent of structure

RELATION OF STAGE AND STORYTELLING

The bachelor thesis deals with stage setting of academic performance Maryso! Evo! Dom! Author analyses the evolution of this stage setting from conceptual preparation to final rehearsing phase. The thesis is also considered on various aspects of stage setting. Author is dealing with relation of stage and actor, stage and theme and also stage and storytelling. In conclusion it is hinting at the issue of transferring the performance to another theatre

Comparison of Communist Successor Parties in Poland and Hungary

The thesis deals with the issue of transformation of communist parties and their subsequent establishment to a democratic world. In theoretic part of the thesis the author follows upon the concept of communist successor party itself, but also examines the differences between communist regimes and strategies of the very transformation. The thesis puts a target in defining the factors and events that direct political heading of those parties. As the subject of investigation the two states and their communist parties were chosen - Poland and Hungary. In those two states particularly the reform wing of the parties gained very prominent position and took control over the democratization and transformation process. After the fall of communism those reform elites became the representatives of the successor parties and started ideological diverging from the extreme left. They began the process of social - democratization, thanks to which those parties had not only survived but also returned to a government shortly. At the final end of the thesis it watches the problematic of the integration of those parties to the European politic structure

Director And Dramatic Text

Diplomová práce se zabývá vztahem režiséra a divadelního textu v jeho různých podobách. V teoretické části vymezuje základní teatrologické pojmy, které se vztahují k části praktické. V té analyzuje tři inscenace a pomocí metody komparace zkoumá na základě několika kritérií úlohu režiséra během přípravy a inscenování jednotlivých forem dramatického textu