71 research outputs found
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
Prokaryotic type II CRISPR-Cas systems can be adapted to enable targeted genome modifications across a range of eukaryotes.1–7. Here we engineer this system to enable RNA-guided genome regulation in human cells by tethering transcriptional activation domains either directly to a nuclease-null Cas9 protein or to an aptamer-modified single guide RNA (sgRNA). Using this functionality we developed a novel transcriptional activation–based assay to determine the landscape of off-target binding of sgRNA:Cas9 complexes and compared it with the off-target activity of transcription activator–like (TAL) effector proteins8, 9. Our results reveal that specificity profiles are sgRNA dependent, and that sgRNA:Cas9 complexes and 18-mer TAL effector proteins can potentially tolerate 1–3 and 1–2 target mismatches, respectively. By engineering a requirement for cooperativity through offset nicking for genome editing or through multiple synergistic sgRNAs for robust transcriptional activation, we suggest methods to mitigate off-target phenomena. Our results expand the versatility of the sgRNA:Cas9 tool and highlight the critical need to engineer improved specificity
Observation of WZ Production
We report the first observation of the associated production of a W boson and
a Z boson. This result is based on 1.1 fb-1 of integrated luminosity from ppbar
collisions at sqrt{s} = 1.96 TeV collected with the CDF II detector at the
Fermilab Tevatron. We observe 16 WZ candidates passing our event selection with
an expected background of 2.7 +/- 0.4 events. A fit to the missing transverse
energy distribution indicates an excess of events compared to the background
expectation corresponding to a significance equivalent to six standard
deviations. The measured cross section is sigma(ppbar -> WZ) =
5.0^{+1.8}_{-1.6} pb, consistent with the standard model expectation.Comment: 7 pages, 3 figures. Submitted to Phys. Rev. Let
Measurement of the Top-Quark Mass in All-Hadronic Decays in p pbar Collisions at CDF II
We present a measurement of the top-quark mass, , in the
all-hadronic decay channel . The analysis is performed using 310 pb of
=1.96 TeV collisions collected with the CDF II detector
using a multi-jet trigger. The mass measurement is based on an event-by-event
likelihood which depends on both the sample purity and the value of the
top-quark mass, using 90 possible jet-to-parton assignments in the six-jet
final state. The joint likelihood of 290 selected events yields a value of
=177.1 4.9 (stat.) 4.7 (syst.) GeV/.Comment: 7 pages, 2 figures and 1 table, Submitted to Phys. Rev. Let
Analysis of the Quantum Numbers of the X(3872) Particle
We present an analysis of angular distributions and correlations of the
X(3872) particle in the exclusive decay mode X(3872)->J/psi pi+ pi- with
J/psi->mu+ mu-. We use 780 pb -1 of data from ppbar collisions at sqrt{s} =
1.96 TeV collected with the CDF II detector at the Fermilab Tevatron. We derive
constraints on spin, parity, and charge conjugation parity of the X(3872)
particle by comparing measured angular distributions of the decay products with
predictions for different JPC hypotheses. The assignments JPC = 1++ and 2-+ are
the only ones consistent with the data.Comment: update to journal versio
Observation of Bs-Bsbar Oscillations
We report the observation of Bs-Bsbar oscillations from a time-dependent
measurement of the Bs-Bsbar oscillation frequency Delta ms. Using a data sample
of 1 fb^-1 of p-pbar collisions at sqrt{s}=1.96 TeV collected with the CDF II
detector at the Fermilab Tevatron, we find signals of 5600 fully reconstructed
hadronic Bs decays, 3100 partially reconstructed hadronic Bs decays, and 61500
partially reconstructed semileptonic Bs decays. We measure the probability as a
function of proper decay time that the Bs decays with the same, or opposite,
flavor as the flavor at production, and we find a signal for Bs-Bsbar
oscillations. The probability that random fluctuations could produce a
comparable signal is 8 X 10^-8, which exceeds 5 sigma significance. We measure
Delta ms = 17.77 +- 0.10 (stat) +- 0.07 (syst) ps^-1
and extract
|Vtd/Vts| = 0.2060 +- 0.0007 (exp) + 0.0081 - 0.0060 (theor).Comment: 9 pages, 5 figures, submitted to Physical Review Letter
Measurement of the Ratio of Branching Fractions B(D0 -> K+ pi-)/B(D0 -> K- pi+) using the CDF II Detector
We present a measurement of R_B, the ratio of the branching fraction for the
rare decay D0 -> K+ pi- to that for the Cabibbo-favored decay D0 -> K- pi+.
Charge conjugate decays are implicitly included. A signal of 2005 +/- 104
events for the decay D0 -> K+ pi- is obtained using the CDF II detector at the
Fermilab Tevatron collider. The data set corresponds to an integrated
luminosity of 0.35 1/fb produced in p-bar/p collisions at sqrt{s}=1.96 TeV.
Assuming no mixing, we find R_B = [ 4.05 +/- 0.21 (stat) +/- 0.11 (syst) ] x
10(-3). This measurement is consistent with the world average, and comparable
in accuracy with the best measurements from other experiments.Comment: 7 pages, 3 figure
Combined Tevatron upper limit on gg->H->W+W- and constraints on the Higgs boson mass in fourth-generation fermion models
Report number: FERMILAB-PUB-10-125-EWe combine results from searches by the CDF and D0 collaborations for a standard model Higgs boson (H) in the process gg->H->W+W- in p=pbar collisions at the Fermilab Tevatron Collider at sqrt{s}=1.96 TeV. With 4.8 fb-1 of integrated luminosity analyzed at CDF and 5.4 fb-1 at D0, the 95% Confidence Level upper limit on \sigma(gg->H) x B(H->W+W-) is 1.75 pb at m_H=120 GeV, 0.38 pb at m_H=165 GeV, and 0.83 pb at m_H=200 GeV. Assuming the presence of a fourth sequential generation of fermions with large masses, we exclude at the 95% Confidence Level a standard-model-like Higgs boson with a mass between 131 and 204 GeV.We combine results from searches by the CDF and D0 collaborations for a standard model Higgs boson (H) in the process gg→H→W+W- in pp̅ collisions at the Fermilab Tevatron Collider at √s=1.96 TeV. With 4.8 fb-1 of integrated luminosity analyzed at CDF and 5.4 fb-1 at D0, the 95% confidence level upper limit on σ(gg→H)×B(H→W+W-) is 1.75 pb at mH=120 GeV, 0.38 pb at mH=165 GeV, and 0.83 pb at mH=200 GeV. Assuming the presence of a fourth sequential generation of fermions with large masses, we exclude at the 95% confidence level a standard-model-like Higgs boson with a mass between 131 and 204 GeV.Peer reviewe
Measurement of the B+ production cross section in p-pbar collisions at sqrt(s) = 1960 GeV
We present a new measurement of the meson differential cross section
at GeV. The data correspond to an integrated
luminosity of 739pb collected with the upgraded CDF detector (CDF II) at
the Fermilab Tevatron collider. candidates are reconstructed through the
decay , with . The integrated cross
section for producing mesons with GeV/c and is
measured to be bComment: 28 pages, 3 tables, 10 figures Submitted to Phys. Rev.
Search for Wprime Boson Decaying to Electron-Neutrino Pairs in pbarp Collisions at sqrt{s}=1.96 TeV
We present the results of a search for Wprime boson decaying to
electron-neutrino pairs in pbarp collisions at a center-of-mass energy of 1.96
TeV, using a data sample corresponding to 205 pb^-1 of integrated luminosity
collected by the CDF II detector at Fermilab. We observe no evidence for this
decay mode and set limits on the production cross section times branching
fraction, assuming the neutrinos from Wprime boson decays to be light. If we
assume the manifest left-right symmetric model, we exclude a Wprime boson with
mass less than 788 GeV/c^2 at the 95% confidence level.Comment: submitted to Physical Review D Rapid Communcatio
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