Geogenomic segregation and temporal trends of human pathogenic Escherichia coli o157:H7, Washington, USA, 2005-2014

The often-noted and persistent increased incidence of Escherichia coli O157:H7 infections in rural areas is not well understood. We used a cohort of E. coli O157:H7 cases reported in Washington, USA, during 2005–2014, along with phylogenomic characterization of the infecting isolates, to identify geographic segregation of and temporal trends in specific phylogenetic lineages of E. coli O157:H7. Kernel estimation and generalized additive models demonstrated that pathogen lineages were spatially segregated during the period of analysis and identified a focus of segregation spanning multiple, predominantly rural, counties for each of the main clinical lineages, Ib, IIa, and IIb. These results suggest the existence of local reservoirs from which humans are infected. We also noted a secular increase in the proportion of lineage IIa and IIb isolates. Spatial segregation by phylogenetic lineage offers the potential to identify local reservoirs and intervene to prevent continued transmission

Measurement of differential t(t)over-bar production cross sections in the full kinematic range using lepton plus jets events from proton-proton collisions at root s=13 TeV

Measurements of differential and double-differential cross sections of top-quark pair (t (t) over bar) production arc presented in the lepton + jets channels with a single electron or muon and jets in the final state. The analysis combines for the first time signatures of top quarks with low transverse momentum p(T), where the top-quark decay products can be identified as separated jets and isolated leptons, and with high p(T), where the decay products are collimated and overlap. The measurements arc based on proton-proton collision data at is - = 13 TeV collected by the CMS experiment at the LHC, corresponding to an integrated luminosity of 137 fb(-1). The cross sections are presented at the parton and particle levels, where the latter minimizes extrapolations based on theoretical assumptions. Most of the measured differential cross sections are well described by standard model predictions with the exception of some double-differential distributions. The inclusive t (t) over bar production cross section is measured to be sigma(t (t) over bar) = 791 +/- 25 pb, which constitutes the most precise measurement in the lepton + jets channel to date.Peer reviewe

EFFECTS OF JOINT PRODUCT MANAGEMENT STRATEGIES ON E.COLI 0157:H7 AND FEEDLOT PROFITS

The objective of this study was to determine the effect of Escherichia coli 0157:H7 on feedlot profits. Fecal samples from 711 feedlot pens in 73 feedlots in Nebraska, Kansas, Oklahoma, and Texas were tested for E. coli 0157:H7. Average daily gain and feed-to-gain ratios were computed for each feedlot pen, and managers from each feedlot provided information on various feedlot management practices. Cattle performance and E. coli 0157:H7 prevalence are both affected by feedlot management practices. The indirect effect of E. coli 0157:H7 on potential feedlot profits was determined by measuring the effects of management practices on E. coli 0157:H7 levels and cattle performance. Management practices that affect cattle performance were identified using ordinary least squares regressions. A negative binomial regression was used to identify management practices that affect E. coli 0157:H7 prevalence. Certain feedlot management practices were identified that have a joint impact on cattle performance and E. coli 0157:H7 prevalence. Using predatory insects to control flies, controlling for stray dogs, foxes, and coyotes in feed areas, removing manure from pens during finishing, and including tallow in the ration were management strategies associated with higher feedlot profits and lower E. coli 0157:H7 prevalence. Using mobile sprinklers for dust control and including alfalfa or sorghum hay or silage in the ration were associated with lower E. coli 0157:H7 prevalence and lower feedlot profits. Increasing days between cleaning water tanks and restricting movement of horses were associated with higher feedlot profits and higher E. coli 0157:H7 levels. Controlling for stray cats in feed areas and including liquid protein in the ration were associated with lower feedlot profits and higher E. coli 0157:H7 levels. These specific management strategies, which were not robust through a sensitivity analysis, should be interpreted with caution. The general categories of management strategies, however, were robust and consistent with past researchLivestock Production/Industries,

Horizontal transmission of Escherichia coli O157:H7 during cattle housing, survival kinetics in feces and water of Escherichia coli O157:H7 and characterisation of E. coli O157:H7 isolates from cattle faeces and a feedlot environment

End of project reportTeagasc acknowledges with gratitude the support of European Union Structural Funds (EAGGF) in financing this research projectEscherichia coli O157:H7 can cause severe illness and in some cases leading to death. Cattle are the main reservoir with transmission to humans occurring through contamination of food or the environment. Improved understanding of the survival and transmission and survival of E. coli O157:H7 on the farm is essential for developing future controls of this pathogen. This study showed that transmission of E. coli O157:H7 can occur rapidly in groups of housed cattle, with contamination of the pens and hides occurring in 24 hrs. The inoculation dose for cattle is lower than previously reported. Ingestion of bacteria from the hide through social grooming is important for pathogen transmission in housed cattle along with faecal contamination of the environment. Sampling hide will improve the estimation of prevalence of E. coli O157:H7 in pens

The metabolic enzyme AdhE controls the virulence of <i>Escherichia coli</i> O157:H7

Classical studies have focused on the role that individual regulators play in controlling virulence gene expression. An emerging theme, however, is that bacterial metabolism also plays a key role in this process. Our previous work identified a series of proteins that were implicated in the regulation of virulence. One of these proteins was AdhE, a bi-functional acetaldehyde-CoA dehydrogenase and alcohol dehydrogenase. Deletion of its gene (adhE) resulted in elevated levels of extracellular acetate and a stark pleiotropic phenotype: strong suppression of the Type Three Secretion System (T3SS) and overexpression of non-functional flagella. Correspondingly, the adhE mutant bound poorly to host cells and was unable to swim. Furthermore, the mutant was significantly less virulent than its parent when tested in vivo, which supports the hypothesis that attachment and motility are central to the colonization process. The molecular basis by which AdhE affects virulence gene regulation was found to be multifactorial, involving acetate-stimulated transcription of flagella expression and post-transcriptional regulation of the T3SS through Hfq. Our study reveals fascinating insights into the links between bacterial physiology, the expression of virulence genes, and the underlying molecular mechanism mechanisms by which these processes are regulated

Microarray-based screening of differentially expressed genes of E. coli O157:H7 Sakai during preharvest survival on butterhead lettuce

Numerous outbreaks of Escherichia coli O157:H7 have been linked to the consumption of leafy vegetables. However, up to the present, little has been known about E. coli O157:H7's adaptive responses to survival on actively growing (and thus responsive) plants. In this study, whole genome transcriptional profiles were generated from E. coli O157:H7 cells (isolate Sakai, stx-) one hour and two days after inoculation on the leaves of growing butterhead lettuce, and compared with an inoculum control. A total of 273 genes of E. coli O157:H7 Sakai (5.04% of the whole genome) were significantly induced or repressed by at least two-fold (p < 0.01) in at least one of the analyzed time points in comparison with the control. Several E. coli O157:H7 genes associated with oxidative stress and antimicrobial resistance were upregulated, including the iron-sulfur cluster and the multiple antibiotic resistance (mar) operon, whereas the Shiga toxin virulence genes were downregulated. Nearly 40% of the genes with significantly different expression were poorly characterized genes or genes with unknown functions. These genes are of special interest for future research as they may play an important role in the pathogens' adaptation to a lifestyle on plants. In conclusion, these findings suggest that the pathogen actively interacts with the plant environment by adapting its metabolism and responding to oxidative stress

CHARACTERIZATION AND GENOMIC ANALYSIS OF TWO ESCHERICHIA COLI O157:H7 BACTERIOPHAGES ISOLATED FROM PIGEONS

Enterohemorrhagic Escherichia coli, also known as EHEC, is a subset of Shiga toxin-producing E. coli (STEC), and it has recently been identified as one of the principal foodborne pathogens. E. coli O157:H7 is the most important serotype of STEC for its role in causing foodborne illnesses. E. coli O157:H7 could cause various gastroenteritis symptoms such as diarrhea, hemolytic uremic syndrome, hemorrhagic colitis, and thrombotic thrombocytopenic purpura and may cause death. Elimination of E. coli O157:H7 during food processing and storage is a possible solution. Bacteriophages have a significant impact on bacterial populations in nature due to their ability to lyse their bacterial host. All E. coli O157:H7 phages that have been previously announced were found in ruminants or swine. Here, I studied the characterization and genomic analysis of two lytic E. coli O157:H7 bacteriophages isolated from feaces of wild pigeon; UAE MI- 01 and Ec_MI-02. This is the first time to report E. coli O157:H7 phages from birds feaces. UAE_MI-01 belonged to the family Siphoviridae in order Caudovirales. UAE_MI-01 had a with a burst size of almost 100 plaque-forming units (PFU) per host cell after a latent period of 20 min and an adsorption rate constant (K) of 1.25 × 10−7 mL/min. UAE_MI-01 was found stable at a wide range of temperature, pH and some of the common laboratory disinfectants. The 44,281 bp-long genomes of the phage had an average GC content of 54.7%. On the other hand, Ec_MI-02 belonged to genus Tequatrovirus in the order Caudovirales. The K value of Ec_MI-02 was found to be 1.55 × 10−8 mL/min. The latent period was almost 50 min with burst size of almost 10 PFU /host cell. Ec_MI-02 was stable at a wide range of pH, and temperature and was resistant to ethanol 70%. Its genome was made of 266 genes with total 165454 bp and average GC content of 35.5%. It is worthy of note that wherever the phage is present, the host cell must be present. Thus, if the bacteriophage of E. coli O157:H7 is present in the feaces of a wild bird, then the E. coli O157:H7, is most probably present in wild birds. The current study provides additional evidence that wild birds could also be a good natural reservoir for bacteriophages and could be good candidates for phage therapy. Studying the genetic makeup of bacteriophages that infect human pathogens is crucial for ensuring their safe usage in the food industry

The production and development of H7 Influenza virus pseudotypes for the study of humoral responses against avian viruses

In recent years, high pathogenicity avian influenza (HPAI) virus, H5N1, low pathogenicity avian influenza (LPAI) virus, H9N2, and both HPAI and LPAI H7 viruses have proved devastating for the affected economies reliant on poultry industry, and have posed serious public health concerns. These viruses have repeatedly caused zoonotic disease in humans, raising concerns of a potential influenza pandemic. Despite the focus on the HPAI H5N1 outbreak in 1997 some H7 strains have also shown to be occasionally adaptable to infecting humans. Therefore, applying knowledge of the H5 virus evolution and spread to the development of sensitive serological methods is likely to improve our ability to understand and respond to the emergence of other HPAI and LPAI viruses, present within the avian populations, with the potential to infect humans and other species. In the present study we describe the construction and production of lentiviral pseudotypes bearing envelope glycoproteins of LPAI and HPAI H7 avian influenza viruses, which have been responsible for several outbreaks in the past decade. The H7 pseudotypes were evaluated in pseudotype-based neutralization (pp-NT) assays in order to detect and quantify the presence of neutralizing antibodies in avian sera, which were confirmed H7 positive by inhibition of haemagglutination (HI) test. Overall, our results substantiate influenza virus pseudotype neutralization as a robust tool for influenza sero-surveillance

Crystal structure of 1,1'-bis[1,7-dicarba-<i>closo</i>-dodecaborane(11)]

In the title compound, C4H22B20, the two {1,7-closo-C2B10H11} cages are linked across a centre of inversion, with C—C = 1.5401 (16) Å. The position of the second non-linking cage C atom was established unambiguously by geometric and crystallographic methods and there is no evidence of C/B disorder

Effect of \u3ci\u3eLactobacillus acidophilus\u3c/i\u3e Strain N P51 on\u3ci\u3e Escherichia coli \u3c/i\u3e0157:H7 Fecal Shedding and Finishing Performance in Beef Feedlot Cattle

A 2-year study was conducted during the summer months (May to September) to test the effectiveness of feeding Lactobacillus acidophilus strain NP51 on the proportion of cattle shedding Escherichia coli 0157:H7 in the feces and evaluate the effect of the treatment on finishing performance. Steers (n = 448) were assigned randomly to pens, and pens of cattle were assigned randomly to NP5 1 supplementation or no supplementation (control). NP5 1 products were mixed with water and applied as the feed was mixed daily in treatment-designated trucks at the rate of l09 CFU per steer. Fecal samples were collected (n = 3,360) from the rectum from each animal every 3 weeks, and E. coli 0157:H7 was isolated by standard procedures, using selective enrichment, immunomagnetic separation, and PCR confirmation. The outcome variable was the recovery of E. coli 0157:H7 from feces, and was modeled using logistic regression accounting for year, repeated measures of pens of cattle, and block. No significant differences were detected for gain, intakes, or feed efficiency of control or NP51-fed steers. The probability for cattle to shed E. coli 0157:H7 varied significantly between 2002 and 2003 (P = 0.004). In 2002 and 2003, the probability for NP5 1-treated steers to shed E. coli 0157:H7 over the test periods was 13 and 21 %, respectively, compared with 21 and 28% among controls. Over the 2 years, NP51-treated steers were 35% less likely to shed E. coli 0157: H7 than were steers in untreated pens (odds ratio = 0.58, P = 0.008). This study is consistent with previous reports that feeding NP51 is effective in reducing E. coli 0157:H7 fecal shedding in feedlot cattle