Experimental determination of the static equivalent pressures of gas phase detonations in pipes and comparison with numerical models

In order to determine the effective load of gas phase detonations on pipe walls ("static equivalent pressure"), comprehensive experiments have been conducted in 48.3×2.6 and 114.3×3.6 pipes (outer diameter [mm] x wall thickness [mm]), in which deflagrative explosions of stoichiometric C2H4/O2/N2-mixtures at 20 °C underwent the transition to detonation. Initial pressures were chosen high enough to produce detonation pressures that caused significant bulging of the pipe walls. All 8 different pressure scenarios that can be distinguished for gas phase detonations in pipes were addressed by the experiments, even the extremely rare case of having the deflagration to detonation transition occurring within about 1 pipe diameter a head of blind fla nge which yields the la rgest static equivalent pressure of all scenarios. By these tests it was possible to (1) validate the predictions of recently developed numerical models for predicting the structural response of the pipe wall and to (2) determine the static equivalent pressure of gas phase detonations in pipes even for those detonative pressure scenarios for which a reliable pressure/space/time profile required as input for the numerical models is at present not yet available. Once the static equivalent pressure is known, the well-established pressure vessel design guidelines, which can only cope with static loads, can be applied for detonation pressure proof pipe design in all those cases where the detonation speed is not close to the propagation speed of the flexural waves in the pipe. Furthermore, preliminary information was obtained about which of the 8 detonative pressure scenarios only depends on the Chapman-Jouguet pressure ratio of the involved mixture and which scenarios will also depend on other characteristic parameters of the involved mixture (difference between initial temperature and auto ignition temperature, ignition delay time). Copyright © 2013 by ASME

A. 1. Teilvorh.: Untersuchung von erster und zweiter Lage von 3He und 4He auf Graphit (mit Adsorptionsisothermen und Neutronenstreuung). B. 2. Teilvorh.: Der Einfluss von Substratsymmetrie auf Phasendiagramme von physisorbierten Adsorbaten Schlussbericht. Zeitraum: 01.10.84-31.12.85

TIB: D.Dt.F./AC 1000 (25,24) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Intrahepatic myeloid-cell aggregates enable local proliferation of CD8⁺ T cells and successful immunotherapy against chronic viral liver infection.

Chronic infection is difficult to overcome because of exhaustion or depletion of cytotoxic effector CD8+ T cells (cytotoxic T lymphoytes (CTLs)). Here we report that signaling via Toll-like receptors (TLRs) induced intrahepatic aggregates of myeloid cells that enabled the population expansion of CTLs (iMATEs: 'intrahepatic myeloid-cell aggregates for T cell population expansion') without causing immunopathology. In the liver, CTL proliferation was restricted to iMATEs that were composed of inflammatory monocyte-derived CD11b+ cells. Signaling via tumor-necrosis factor (TNF) caused iMATE formation that facilitated costimulation dependent on the receptor OX40 for expansion of the CTL population. The iMATEs arose during acute viral infection but were absent during chronic viral infection, yet they were still induced by TLR signaling. Such hepatic expansion of the CTL population controlled chronic viral infection of the liver after vaccination with DNA. Thus, iMATEs are dynamic structures that overcome regulatory cues that limit the population expansion of CTLs during chronic infection and can be used in new therapeutic vaccination strategies

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion