A long-term copper exposure on freshwater ecosystem using lotic mesocosms: Individual and population responses of three-spined sticklebacks (Gasterosteus aculeatus)

Three-spined stickleback (Gasterosteus aculeatus) was used as the highest trophic level predator in an outdoor mesocosm study assessing the effect of environmentally realistic copper concentration (0, 5, 25 and 75 μg L−1) over 18 months of continuous exposure. Condition factor, organosomatic indices (HIS, GSI and SSI) as well as copper bioaccumulation in the liver were measured at 15 days, 2, 4, 6, 10, 14 and 18 months after the beginning of the contamination. Population monitoring was realised after 6 and 18 months of contamination, allowing two reproduction periods to be measured. Results showed that condition factor was affected at medium and high copper concentrations and HSI was sporadically affected in all copper exposure, depending on the sex of the fish. GSI did not show any significant differences and SSI was lowered in the medium and high copper levels. Bioaccumulation was significantly different in males and females and fluctuated with season. A negative correlation was observed between copper bioaccumulation in the liver and fish size and a positive correlation with nominal copper concentration in the water was found. There was a negative correlation between condition factor, organosomatic indices and bioaccumulation in the liver. Population monitoring showed a significantly higher fish mean length after 6 months and a higher abundance after 18 months of exposure at the highest copper level. We conclude that indirect effects such as food and habitat availability or lower predation pressure on eggs and juveniles might have led to higher stickleback population abundances at the highest copper level. This highlights the need to study all the trophic levels when monitoring ecosystem health. Considering the population and the individual responses after 18 months of copper exposure, the NOEC for three-spined sticklebacks was 25 μg L−1 (or 20 μg L−1 if we consider the average effective concentration), with a LOEC of 75 μg L−1 (or 57 μg L−1, AEC)

Alteration of leaf decomposition in copper-contaminated freshwater mesocosms

The influence of copper on leaf litter decomposition was examined in experimental streams. Controls and three levels of contamination (5, 25, and 75 microg/L) were tested in triplicate in 20-m-long mesocosms. Equal quantities of alder, maple, and oak leaves were enclosed in mesh bags and placed in the upper and lower mesocosm sections that exhibited different habitat characteristics (shallow with pebble substrate or deep with fine substrate and macrophytes, respectively). Decomposition rate in the upper section of the 75 micro.g/L mesocosms was significantly reduced to 28% of control values. No significant reductions in the decomposition rate were seen in the lower section. No differences in the leaf-associated mycelial biomass and sporulation rates were observed between treatments, but they were consistently higher in the upper sections. The aquatic hyphomycete community was not affected by the contamination. The abundances of total leaf-associated macroinvertebrates and the dominant shredder (Gammarus pulex) were considerably reduced in the upper sections at 75 microg/L. Therefore, the reduced leaf decomposition probably resulted from a reduction in the abundance of macroinvertebrate detritivores. In addition, local variations in physical and biological characteristics of the habitat along the mesocosms significantly influenced the effects of copper on leaf-associated decomposers

Atypical Distant Metastasis of Breast Malignant Phyllodes Tumors: A Case Report and Literature Review

Malignant phyllodes tumors (MPT) are rare breast neoplasms. Preoperative diagnosis is often challenging due to the unspecific clinical, radiological, and histological characteristics of the tumor. Dissemination pathways are local with chest wall invasion, regional with lymph nodes metastasis, and distant, hematogenous, mostly to the lungs, bones, and brain. Distant metastasis (DM) can be synchronous or appear months to years after the diagnosis and initial management. The current report describes the case of a 57-year-old woman presenting with a giant/neglected MPT of the breast, with no DM at initial staging, treated by radical modified mastectomy. Motor disorders due to medullar compression by a paravertebral mass appeared at short follow-up, also treated surgically. The patient died from several DM of rapid evolution. To our knowledge, this is the only case described of MPT with metastases to soft tissue causing medullar compression. We present a literature review on unusual metastatic localizations of MPT

Using plant litter decomposition as an indicator of ecosystem response to soil contamination

The inventory and remediation of contaminated sites have emerged as top environmental priorities worldwide. A large body of evidence has accumulated to show how soil contamination affects biological communities and ecological processes. This knowledge has yet to be used for the development of indicators of soil quality that are meaningful to end-users and are easy to implement in soil quality assessment schemes. In this study, we used quantifiable measures of litter decomposition, a key biophysical process, as indicators of the ecological impact of soil contamination by trace metals and hydrocarbons. We conducted a litterbag experiment with coarse and fine mesh bags to compare highly vs. minimally contaminated sites within eight locations representative of a wide array of environmental conditions and types of pollution. Contrary to the common assumption that soil contamination hampers soil functions, idiosyncratic responses were detected for litter decomposition rate and decomposer activity metrics. A negative relationship between detritivore and microbial responses to soil contamination indicates that wherever the activity of one group of decomposers is reduced, increase in activity of the other group may ensure litter decomposition to proceed at rate similar or higher than baseline rate. This finding may indicate that compensatory dynamics in soil communities is important in determining ecosystem stability against chemical stressors. As litter decomposition may inform on the capacity of terrestrial ecosystems to cope with soil contamination, it may be a useful complement to chemical soil analyses in routine soil quality assessment schemes

Development of an Anthropomorphic Phantom of the Axillary Region for Microwave Imaging Assessment

We produced an anatomically and dielectrically realistic phantom of the axillary region to enable the experimental assessment of Axillary Lymph Node (ALN) imaging using microwave imaging technology. We segmented a thoracic Computed Tomography (CT) scan and created a computer-aided designed file containing the anatomical configuration of the axillary region. The phantom comprises five 3D-printed parts representing the main tissues of interest of the axillary region for the purpose of microwave imaging: fat, muscle, bone, ALNs, and lung. The phantom allows the experimental assessment of multiple anatomical configurations, by including ALNs of different size, shape, and number in several locations. Except for the bone mimicking organ, which is made of solid conductive polymer, we 3D-printed cavities to represent the fat, muscle, ALN, and lung and filled them with appropriate tissue-mimicking liquids. Existing studies about complex permittivity of ALNs have reported limitations. To address these, we measured the complex permittivity of both human and animal lymph nodes using the standard open-ended coaxial-probe technique, over the 0.5 GHz-8.5 GHz frequency band, thus extending current knowledge on dielectric properties of ALNs. Lastly, we numerically evaluated the effect of the polymer which constitutes the cavities of the phantom and compared it to the realistic axillary region. The results showed a maximum difference of 7 dB at 4 GHz in the electric field magnitude coupled to the tissues and a maximum of 10 dB difference in the ALN response. Our results showed that the phantom is a good representation of the axillary region and a viable tool for pre-clinical assessment of microwave imaging technology.info:eu-repo/semantics/publishedVersio

Rapid thermal processing of CuInSe2 electroplated precursors for CuIn(S,Se)2-based thin film solar cells

International audienceDuring the elaboration of standard CISEL™cells, electroplated CuInSe2 precursors undergo a rapid thermal processing (RTP) in a sulfur-containing atmosphere to promote grain growth and enable sulfurization of the precursor. The aim of this work is to show how structural and morphological properties of the CuIn(S,Se)2-based solar cells can be modified with RTP parameters, namely temperature, heating rate, and sulfur addition. X-ray diffractograms show that the preferential (112) orientation of the electrodeposited CuInSe2 precursor is maintained after annealing but the coefficient of crystallographic texture can be modified with specific RTP parameters. It is also shown that the quantity of sulfur incorporated in the chalcopyrite lattice can be controlled and reaches almost pure CuInS2 according to the sulfur quantity used during the RTP. Another effect of the RTP annealing is to form a Mo(S,Se)2 layer which can lead to a quasi-ohmic contact between the molybdenum and the absorber. The properties of the Mo(S,Se)2 buffer layer are also studied according to the process parameters and an increase of the annealing temperature or of the sulfur concentration tends to increase the thickness of this laye

CANABIC: CANnabis and Adolescents: effect of a Brief Intervention on their Consumption - study protocol for a randomized controlled trial.

International audienceBACKGROUND: Cannabis is the most consumed illegal substance in France. General practitioners (GPs) are the health professionals who are most consulted by adolescents. Brief intervention (BI) is a promising care initiative for the consumption of cannabis, and could be a tool for GPs in caring for adolescents who consume cannabis. The aim of the CANABIC study is to measure the impact of a BI carried out by a GP on the consumption of cannabis by adolescents of 15 to 25 years of age. METHODS: A randomized clustered controlled trial, stratified over three areas (Auvergne, Languedoc-Roussillon, and Rhone-Alps), comparing an intervention group, which carries out the BI in consultation, and a control group, which ensures routine medical care. The main assessment criterion is the consumption of cannabis by amount of joints per month, at 12 months. The amount necessary to highlight a significant difference between the two groups of 30 % of consumption at 12 months is 250 patients (50 GPs, 5 patients per GP; risk alpha = 5 %; power = 90 %; intra-cluster correlation coefficient rho = 0.2; Hawthorne effect = 15 %; lost to follow-up rates for GPs = 10 % and for patients = 20 %). This plan is replicated for the three areas, and therefore a total of 750 patients are expected.The secondary criteria for judgment are the associated consumption of tobacco and alcohol, the perception of the consequences of consumption, and the driving of a vehicle following consumption. DISCUSSION: Research about BI for young cannabis users is underway. The aim of the CANABIC study is to validate a BI suited to adolescents who consume cannabis, which may be performed in the general practice. This would provide a tool for their treatment by a GP, which could be widely distributed during initial or further medical training.Trial registration: CANABIC is a randomized clustered trial (NCT01433692, registered 2011 Sept 12), PHRC funded: Clinical Research Hospital Program (Governmental Fund, Health Ministry). Date first patient randomized: March 2012

Spatially resolved stellar, dust and gas properties of the post-interacting Whirlpool Galaxy system

Using infrared imaging from the Herschel Space Observatory, observed as part of the VNGS, we investigate the spatially resolved dust properties of the interacting Whirlpool galaxy system (NGC 5194 and NGC 5195), on physical scales of 1 kpc. Spectral energy distribution modelling of the new infrared images in combination with archival optical, near- through mid-infrared images confirms that both galaxies underwent a burst of star formation ~370-480 Myr ago and provides spatially resolved maps of the stellar and dust mass surface densities. The resulting average dust-to-stellar mass ratios are comparable to other spiral and spheroidal galaxies studied with Herschel, with NGC 5194 at log M(dust)/M(star)= -2.5+/-0.2 and NGC 5195 at log M(dust)/M(star)= -3.5+/-0.3. The dust-to-stellar mass ratio is constant across NGC 5194 suggesting the stellar and dust components are coupled. In contrast, the mass ratio increases with radius in NGC 5195 with decreasing stellar mass density. Archival mass surface density maps of the neutral and molecular hydrogen gas are also folded into our analysis. The gas-to-dust mass ratio, 94+/-17, is relatively constant across NGC 5194. Somewhat surprisingly, we find the dust in NGC 5195 is heated by a strong interstellar radiation field, over 20 times that of the ISRF in the Milky Way, resulting in relatively high characteristic dust temperatures (~30 K). This post-starburst galaxy contains a substantial amount of low-density molecular gas and displays a gas-to-dust ratio (73+/-35) similar to spiral galaxies. It is unclear why the dust in NGC 5195 is heated to such high temperatures as there is no star formation in the galaxy and its active galactic nucleus is 5-10 times less luminous than the one in NGC 5194, which exhibits only a modest enhancement in the amplitude of its ISRF.Comment: 26 pages, 24 figures, Accepted for publication in Ap