Tryptophan requirements and the effects of supplemental tryptophan on growth performance, plasma metabolites, and meat quality in nursery, growing, and finishing pigs

This research was conducted to estimate the true digestible Trp (dTrp) requirements in nursery, growing, and late finishing pigs and the effects of supplemental Trp on physiology, behavior, and meat quality. Five experiments were conducted to estimate the dTrp requirement in nursery pigs. Using broken-line regression analysis, dTrp requirements were 0.21, 0.20, and 0.18% for Phase I (5.2 to 7.3 kg), II (6.3 to 10.2 kg), and III (10.3 to 15.7) nursery pigs. In addition, four experiments were conducted to estimate the dTrp requirements in growing and finishing pigs. Using broken-line regression analysis, the dTrp requirement of pigs weighing 30.9, 51.3, and 74.6 to 104.5 kg was 0.167, 0.134, and 0.102%, respectively. An experiment also was conducted to determine the ratio of Trp:Lys and Thr:Lys in diets for nursery pigs (7.1 to 15.6 kg BW). The treatments were arranged in a 3 x 3 factorial with three ratios of true digestible Thr:Lys (0.55, 0.60, or 0.65) and three ratios of true digestible Trp:Lys (0.145, 0.170, or 0.195). Overall, optimal performance was in pigs fed the true digestible Trp:Lys ratio of 0.195 at Thr:Lys ratios 0.60 and 0.65. These results indicate that dietary levels of Trp above 0.19% may be needed to maximize growth performance in diets containing wheat and barley. Four experiments were conducted to determine the effects of supplemental Trp on meat quality and plasma and salivary cortisol and plasma lactate in growing pigs. Pigs fed the diet with supplemental Trp had lower (P \u3c 0.01) mean plasma cortisol and lactate (P \u3c 0.07) concentration than pigs fed the basal diet. Meat quality effects varied, but overall, results indicated that Trp had no positive effect on meat quality. Lastly, a study was conducted to evaluate the effects of Trp on growth, behavior, intestinal morphology, and brain and plasma metabolites subsequent to weaning and mixing. Cortisol was decreased (P \u3c 0.07) after mixing in pigs fed Trp. Brain metabolites also were increased (P \u3c 0.09) by Trp. Tryptophan supplementation has varied effects on growth performance, behavior, physiology, and meat quality in pigs

Introduction to the SalSA, a saltdome shower array as a GZK neutrino observatory

The observed spectrum of ultra-high energy cosmic rays virtually guarantees the presence of ultra-high energy neutrinos due to their interaction with the cosmic microwave background. Every one of these neutrinos will point back to its source and, unlike cosmic rays, will arrive at the Earth unattenuated, from sources perhaps as distant as z=20. The neutrino telescopes currently under construction, should discover a handful of these events, probably too few for detailed study. In this talk I will describe how an array of VHF and UHF antennas embedded in a large salt dome, SalSA (Saltdome Shower Array) promises to yield a teraton detector (\u3e 500 km3-sr) for contained neutrino events with energies above 1017 eV. Our simulations show that such a detector may observe several hundreds of these neutrinos over its lifetime. Our simulations also show how such interactions will provide high energy physicists with an energy frontier for weak interactions an order-of-magnitude larger than that of the LHC. The flavor ID capalities of SALSA, combined with the extreme L/E of these neutrinos, will provide a window on neutrino oscillations and decay times eight orders of magnitude higher than laboratory experiments. In addition to the latest simulation results, we describe progress on detectors and site selection. © World Scientific Publishing Company

Blunted nutrient-response pathways in adipose tissue following high fat meals in men with metabolic syndrome: A randomized postprandial transcriptomic study

BACKGROUND: Excessive adipose tissue is central to disease burden posed by the Metabolic Syndrome (MetS). Whilst much is known of the altered transcriptomic regulation of adipose tissue under fasting conditions, little is known of the responses to high-fat meals. METHODS: Nineteen middle-aged males (mean ± SD 52.0 ± 4.6 years), consumed two isocaloric high-fat, predominately dairy-based or soy-based, breakfast meals. Abdominal subcutaneous adipose biopsies were collected after overnight fast (0 h) and 4 h following each meal. Global gene expression profiling was performed by microarray (Illumina Human WG-6 v3). RESULTS: In the fasted state, 13 genes were differently expressed between control and MetS adipose tissue (≥1.2 fold-difference, p < 0.05). In response to the meals, the control participants had widespread increases in genes related to cellular nutrient responses (≥1.2 fold-change, p < 0.05; 2444 & 2367 genes; dairy & soy, respectively). There was blunted response in the MetS group (≥1.2 fold-change, p < 0.05; 332 & 336 genes; dairy & soy, respectively). CONCLUSIONS: In middle-aged males with MetS, a widespread suppression of the subcutaneous adipose tissue nutrient responsive gene expression suggests an inflexibility in the transcriptomic responsiveness to both high-fat meals

Results of the ICTuS 2 Trial (Intravascular Cooling in the Treatment of Stroke 2)

Background and purposeTherapeutic hypothermia is a potent neuroprotectant approved for cerebral protection after neonatal hypoxia-ischemia and cardiac arrest. Therapeutic hypothermia for acute ischemic stroke is safe and feasible in pilot trials. We designed a study protocol to provide safer, faster therapeutic hypothermia in stroke patients.MethodsSafety procedures and 4°C saline infusions for faster cooling were added to the ICTuS trial (Intravascular Cooling in the Treatment of Stroke) protocol. A femoral venous intravascular cooling catheter after intravenous recombinant tissue-type plasminogen activator in eligible patients provided 24 hours cooling followed by a 12-hour rewarm. Serial safety assessments and imaging were performed. The primary end point was 3-month modified Rankin score 0,1.ResultsOf the intended 1600 subjects, 120 were enrolled before the study was stopped. Randomly, 63 were to receive hypothermia plus antishivering treatment and 57 normothermia. Compared with previous studies, cooling rates were improved with a cold saline bolus, without fluid overload. The intention-to-treat primary outcome of 90-day modified Rankin Score 0,1 occurred in 33% hypothermia and 38% normothermia subjects, odds ratio (95% confidence interval) of 0.81 (0.36-1.85). Serious adverse events occurred equally. Mortality was 15.9% hypothermia and 8.8% normothermia subjects, odds ratio (95% confidence interval) of 1.95 (0.56-7.79). Pneumonia occurred in 19% hypothermia versus 10.5% in normothermia subjects, odds ratio (95% confidence interval) of 1.99 (0.63-6.98).ConclusionsIntravascular therapeutic hypothermia was confirmed to be safe and feasible in recombinant tissue-type plasminogen activator-treated acute ischemic stroke patients. Protocol changes designed to reduce pneumonia risk appeared to fail, although the sample is small.Clinical trial registrationURL: http://www.clinicaltrials.gov. Unique identifier: NCT01123161